LOCUS       40924_24273       10974 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pHC71, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10974)
  AUTHORS   Chou H-H., Berthet J, Marx CJ.
  TITLE     Growth rate modulates the selective advantage of recurrent 
            transposition mutations arising from experimental populations grown 
            in a metal-limited environment
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10974)
  AUTHORS   Chou H-H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2008) Organismic and Evolutionary Biology, Harvard
            University, 16 Divinity Avenue, Biolab 3079, Cambridge, MA 02138, 
            USA
REFERENCE   3  (bases 1 to 10974)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10974)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (15-OCT-2008) Organismic and Evolutionary Biology, Harvard 
            University, 16 Divinity Avenue, Biolab 3079, Cambridge, MA 02138, 
            USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10974
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      3..712
                     /label=oriV
                     /note="incP origin of replication"
     rep_origin      1105..1693
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    1981..2002
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2017..2047
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2055..2071
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2079..2095
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(2133..2864)
                     /codon_start=1
                     /gene="icuB"
                     /product="IcuB"
                     /label=icuB
                     /protein_id="ACR44971.1"
                     /translation="MRRTVILTALLAFTAPAAAHDLWLDPAGNGVQILYGHPHEPELPS
                     AGKLMSLTAYEPSGAVALNAKLQTGAMPALKAAHQGDALFAAAYDNGYWVRLSDGGYRN
                     ASKRMLPQADKSLWSVKFAKAVSGPTAPWDKIVGQPLEIVPLEEPAAASGQIRVRVLFE
                     GRPLDGASVVATDGVNFKSEADQARATTDADGVAVVSLRSAGPQVLGVAHRVSPSQTPA
                     LADADSYGATLAFTVTDPKSN"
     gene            complement(2133..2864)
                     /gene="icuB"
                     /label=icuB
     CDS             complement(2861..4975)
                     /codon_start=1
                     /gene="icuA"
                     /product="IcuA"
                     /label=icuA
                     /protein_id="ACR44972.1"
                     /translation="MSPLLTRLLASASALGVSTLPSHALDPTAVELEELQVTAQGGSAN
                     SVAAVAGPAANLKTQISPSVLALPGNSTVIDAAEIARLPVQSYGDLFRPVAGFDVSNYG
                     QGGVGYGIALRGFSDAEHGRDVAVFIDGVPINEVSSIHTPNYVDLNPLIPETIRSISIV
                     RGPFSVEAGDANLGGSIFIETKRAEPFSTVGASGGSFGTWRGLATFSQIGPGIQPFLAY
                     EGYGTGGYRENGDLGRYNAFNKVTIPLDLGSSLSLRVQMYGTGFGQPGYLERDRVRAGL
                     VSPRAATNITDGGGKTLQTFVANYVNGLPDDELSTLLYASHDVFSRFSDFGAGQRGQVE
                     ERETVGGRIRKVWTTSLFDVLPTQVLVGGDWRSDALDVNTGPSVARTFTARTLDLDLSQ
                     HNLAAYAQVQVKPAPWLKLTGGARFDQFFYDVRNNLDPALSPKVSPNVVSPKGGAALTP
                     FDWLELFANYGQGFRSPSASSELTNNPRLTPLTIESTEGGGRLVFDRFSLLGTVFFTDI
                     ANEVFQPAPGLPVQNLGKSRRDGFEIEAKVLAYREGLDRVALFANYSGVSARLLNGAPS
                     IFIPNVPAYLATVGAEFDVAAPAALLPGGRLTGAAYLSYIGAKPLTEDGVLHSSPYPRV
                     SGRIAYVHQSGWTTYGQATWYPGDRTSESIFNFGSSVSASAAEIVTSPQPRLTLLAGIT
                     SSFATGAPLR"
     gene            complement(2861..4975)
                     /gene="icuA"
                     /label=icuA
     promoter        complement(5003..5031)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     terminator      complement(5145..5172)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     terminator      complement(5264..5350)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     primer_bind     complement(5532..5548)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(5993..6634)
                     /codon_start=1
                     /label=TetR
                     /note="tetracycline resistance regulatory protein"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKG"
     CDS             complement(7415..8227)
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(8902..10047)
                     /codon_start=1
                     /label=trfA
                     /note="trans-acting replication protein that binds to and 
                     activates oriV"
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(10319..10690)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSL*AYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            complement(10723..10832)
                     /direction=LEFT
                     /label=oriT
                     /note="incP origin of transfer"
ORIGIN
        1 gaccctttcc gacgctcacc gggctggttg ccctcgccgc tgggctggcg gccgtctatg
       61 gccctgcaaa cgcgccagaa acgccgtcga agccgtgtgc gagacaccgc ggccgccggc
      121 gttgtggata cctcgcggaa aacttggccc tcactgacag atgaggggcg gacgttgaca
      181 cttgaggggc cgactcaccc ggcgcggcgt tgacagatga ggggcaggct cgatttcggc
      241 cggcgacgtg gagctggcca gcctcgcaaa tcggcgaaaa cgcctgattt tacgcgagtt
      301 tcccacagat gatgtggaca agcctgggga taagtgccct gcggtattga cacttgaggg
      361 gcgcgactac tgacagatga ggggcgcgat ccttgacact tgaggggcag agtgctgaca
      421 gatgaggggc gcacctattg acatttgagg ggctgtccac aggcagaaaa tccagcattt
      481 gcaagggttt ccgcccgttt ttcggccacc gctaacctgt cttttaacct gcttttaaac
      541 caatatttat aaaccttgtt tttaaccagg gctgcgccct gtgcgcgtga ccgcgcacgc
      601 cgaagggggg tgccccccct tctcgaaccc tcccggcccg ctaacgcggg cctcccatcc
      661 ccccaggggc tgcgcccctc ggccgcgaac ggcctcaccc caaaaatggc agccaagctg
      721 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
      781 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
      841 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
      901 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
      961 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     1021 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     1081 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     1141 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     1201 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt
     1261 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     1321 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     1381 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     1441 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     1501 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     1561 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     1621 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     1681 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     1741 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
     1801 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
     1861 cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca
     1921 ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat
     1981 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
     2041 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga
     2101 ttacgccaag cttgcatgcc taggctagcc gctcagttgg acttcgggtc ggtcacggtg
     2161 aaggcgagcg tcgcgccgta gctgtccgca tcggccagcg ccggcgtctg cgaggggctc
     2221 acgcggtgcg ccacgccgag cacctgggga ccggcactgc gcagcgagac gaccgccacg
     2281 ccgtccgcat ccgtcgtggc gcgggcctga tccgcctcgc tcttgaagtt cacgccgtcg
     2341 gtggcgacca cgctggcgcc gtcgagcgga cggccctcga acagcacccg cacccggatc
     2401 tggcccgaag ccgcggccgg ctcctccagc gggacgatct ccagcggttg gccgacgatc
     2461 ttgtcccacg gggccgtcgg gccggatacg gccttggcga acttcaccga ccagaggctc
     2521 ttgtcggcct gcggcagcat ccgcttgctg gcgttgcggt agccgccgtc ggacaagcgc
     2581 acccagtagc cgttgtcgta ggccgcggcg aacagcgcgt cgccctgatg cgcggctttg
     2641 agcgcgggca ttgcgccggt ctgcagcttg gcattcaggg ccaccgcgcc ggacggctcg
     2701 taggcggtca ggctcatcag cttgccagca ctcggcagtt ccggctcgtg gggatgcccg
     2761 tagaggatct gcacgccgtt gccggccggg tcgagccaga ggtcgtgggc ggcggccggt
     2821 gcggtgaagg cgagcagcgc ggtgaggatg acggttcgtc tcatcgaaga ggtgctcctg
     2881 ttgcaaagct ggaggtgatg ccggccagca gcgtcaggcg cggctgcggg ctggtgacga
     2941 tctcggcggc cgaggcagag accgacgagc cgaagttgaa gatgctctcg ctcgtgcggt
     3001 cgccgggata ccaggtggcc tgcccgtagg tcgtccagcc gctctggtgg acgtaggcga
     3061 tccggccgct gacgcgcgga tagggcgagg aatgcaatac gccgtcctcg gtcaacggct
     3121 tggcaccgat gtaggacagg taggccgcac cggtcaggcg cccgccgggc aacagggcgg
     3181 ccggcgcggc gacgtcgaat tcggcgccga cggtggcaag atacgccggc acgttgggga
     3241 tgaagatgga tggcgcgccg ttgagcagtc gggccgagac gcccgaatag ttggcgaaca
     3301 acgccacgcg gtcgaggcct tcacgatagg ccagcacctt cgcctcgatt tcgaagccgt
     3361 cgcggcggga cttgcccagg ttctgcacgg gcaggccggg tgcgggctgg aagacctcgt
     3421 tggcgatgtc ggtgaagaac accgtgccga ggagactgaa ccggtcgaat acgaggcggc
     3481 cgccgccctc cgtactctcg atggtcagcg gagtgaggcg gggattgttc gtcaattcgc
     3541 tcgaagcgga agggctgcgg aagccctggc cgtaattggc gaacagctcc agccagtcga
     3601 agggcgtgag cgccgccccg cccttggggg agaccacgtt ggggctgacc ttgggcgaca
     3661 gggccgggtc gagattgttg cgcacgtcgt agaagaactg gtcgaagcgg gcgccgccgg
     3721 tcagcttcag ccagggcgcc ggcttgacct gcacctgcgc gtaggccgcg aggttgtgct
     3781 gcgagagatc gagatcgagg gtgcgtgcgg tgaacgtgcg cgcgaccgag gggccggtgt
     3841 tcacgtcgag cgcgtcgctc cgccaatcgc cgccgacgag gacttgggtc ggcagcacgt
     3901 cgaacaggct cgtcgtccag accttgcgga tgcggccacc cacggtctcc cgttcctcga
     3961 cctgtccgcg ctggccggcg ccgaaatccg agaagcggct gaacacgtcg tggctggcgt
     4021 agagaagcgt cgacagctcg tcgtctggaa gcccgttgac gtagttggcc acgaaggtct
     4081 gcagggtctt gccgccgcca tcggtgatgt tggtcgcggc ccgcggtgag acgagaccgg
     4141 cgcgcacgcg gtcgcgctcc aggtagcccg gctgcccgaa ccccgtgccg tacatctgca
     4201 cccgcagcga caggctggag ccgagatcga gggggatggt gaccttgttg aaggcgttgt
     4261 agcgcccgag atcgccgttc tcgcgatagc ccccggtgcc gtagccctcg taggcgagga
     4321 agggctggat ccccgggccg atctggctga aggtggcaag cccccgccac gtaccgaacg
     4381 agccgcccga tgcgccgacg gtcgagaagg gctcggcccg cttggtctcg atgaagatcg
     4441 agccgccgag attggcgtcg cccgcttcga ccgagaacgg gccgcgcacg atgctgatgg
     4501 agcggatggt ctccgggatc agcggattga gatcgacgta gttcggggtg tggatcgagg
     4561 agacctcgtt gatcggcacc ccgtcgatga aaacggcgac gtcgcgaccg tgctcggcat
     4621 cgctgaagcc gcgcagggcg atgccgtatc cgaccccgcc ctgcccgtaa ttcgagacat
     4681 cgaagcctgc cacgggacgg aagaggtcgc cgtagctttg caccggcagg cgcgcgatct
     4741 ccgcggcatc gatcaccgtg ctgttgcccg gcagggccag gaccgatggt gagatctggg
     4801 tcttcaggtt cgcggccggt cccgcaaccg ccgcaacgga atttgcggaa ccgccctgcg
     4861 cggtgacctg tagttcctcc agctcaactg cggtcggatc gagcgcatgg ctcggaagcg
     4921 tactcacgcc gagtgcgctg gccgatgcga gcaggcgcgt cagaagcggc gacattcggg
     4981 gtctctccct ggtacctcca cacattatac gagccgatga ttaattgtca acagctcatt
     5041 tcagagaatt ccctgctttc ctgatgcaaa aacgaggcta gtttaccgta tctgtggggg
     5101 gatggcttgt agatatgacg acaggaagag tttgtagaaa cgcaaaaagg ccatccgtca
     5161 ggatggcctt ctgcttaatt tgatgcctgg cagtttatgg cgggcgtcct gcccgccacc
     5221 ctccgggccg ttgcttcgca acgttcaaat ccgctcccgg cggatttgtc ctactcagga
     5281 gagcgttcac cgacaaacaa cagataaaac gaaaggccca gtctttcgac tgagcctttc
     5341 gttttatttg atgcctggca gttccctact ctcgcatggg gagaccccac actaccatcg
     5401 gcgctacggc gtttcacttc tgagttcggc atggggtcag gtgggaccac cgcgctactg
     5461 ccgccaggca aattctgttt tatcagaccg cttctgcgtt ctgatttaat ctgtatcagg
     5521 ctgaaaaatt cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc
     5581 caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc
     5641 cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggcg cctgatgcgg
     5701 tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatggtgcac tctcagtaca
     5761 atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg
     5821 ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg
     5881 agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc
     5941 gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gcaccctttc
     6001 tcggtccttc aacgttcctg acaacgagcc tccttttcgc caatccatcg acaatcaccg
     6061 cgagtccctg ctcgaacgct gcgtccggac cggcttcgtc gaaggcgtct atcgcggccc
     6121 gcaacagcgg cgagagcgga gcctgttcaa cggtgccgcc gcgctcgccg gcatcgctgt
     6181 cgccggcctg ctcctcaagc acggccccaa cagtgaagta gctgattgtc atcagcgcat
     6241 tgacggcgtc cccggccgaa aaacccgcct cgcagaggaa gcgaagctgc gcgtcggccg
     6301 tttccatctg cggtgcgccc ggtcgcgtgc cggcatggat gcgcgcgcca tcgcggtagg
     6361 cgagcagcgc ctgcctgaag ctgcgggcat tcccgatcag aaatgagcgc cagtcgtcgt
     6421 cggctctcgg caccgaatgc gtatgattct ccgccagcat ggcttcggcc agtgcgtcga
     6481 gcagcgcccg cttgttcctg aagtgccagt aaagcgccgg ctgctgaacc cccaaccgtt
     6541 ccgccagttt gcgtgtcgtc agaccgtcta cgccgacctc gttcaacagg tccagggcgg
     6601 cacggatcac tgtattcggc tgcaactttg tcatgattga cactttatca ctgataaaca
     6661 taatatgtcc accaacttat cagtgataaa gaatccgcgc gttcaatcgg accagcggag
     6721 gctggtccgg aggccagacg tgaaacccaa catacccctg atcgtaattc tgagcactgt
     6781 cgcgctcgac gctgtcggca tcggcctgat tatgccggtg ctgccgggcc tcctgcgcga
     6841 tctggttcac tcgaacgacg tcaccgccca ctatggcatt ctgctggcgc tgtatgcgtt
     6901 ggtgcaattt gcctgcgcac ctgtgctggg cgcgctgtcg gatcgtttcg ggcggcggcc
     6961 aatcttgctc gtctcgctgg ccggcgccac tgtcgactac gccatcatgg cgacagcgcc
     7021 tttcctttgg gttctctata tcgggcggat cgtggccggc atcaccgggg cgactggggc
     7081 ggtagccggc gcttatattg ccgatgacct gcaggggggg gggggcgctg aggtctgcct
     7141 cgtgaagaag gtgttgctga ctcataccag gcctgaatcg ccccatcatc cagccagaaa
     7201 gtgagggagc cacggttgat gagagctttg ttgtaggtgg accagttggt gattttgaac
     7261 ttttgctttg ccacggaacg gtctgcgttg tcgggaagat gcgtgatctg atccttcaac
     7321 tcagcaaaag ttcgatttat tcaacaaagc cgccgtcccg tcaagtcagc gtaatgctct
     7381 gccagtgtta caaccaatta accaattctg attagaaaaa ctcatcgagc atcaaatgaa
     7441 actgcaattt attcatatca ggattatcaa taccatattt ttgaaaaagc cgtttctgta
     7501 atgaaggaga aaactcaccg aggcagttcc ataggatggc aagatcctgg tatcggtctg
     7561 cgattccgac tcgtccaaca tcaatacaac ctattaattt cccctcgtca aaaataaggt
     7621 tatcaagtga gaaatcacca tgagtgacga ctgaatccgg tgagaatggc aaaagcttat
     7681 gcatttcttt ccagacttgt tcaacaggcc agccattacg ctcgtcatca aaatcactcg
     7741 catcaaccaa accgttattc attcgtgatt gcgcctgagc gagacgaaat acgcgatcgc
     7801 tgttaaaagg acaattacaa acaggaatcg aatgcaaccg gcgcaggaac actgccagcg
     7861 catcaacaat attttcacct gaatcaggat attcttctaa tacctggaat gctgttttcc
     7921 cggggatcgc agtggtgagt aaccatgcat catcaggagt acggataaaa tgcttgatgg
     7981 tcggaagagg cataaattcc gtcagccagt ttagtctgac catctcatct gtaacatcat
     8041 tggcaacgct acctttgcca tgtttcagaa acaactctgg cgcatcgggc ttcccataca
     8101 atcgatagat tgtcgcacct gattgcccga cattatcgcg agcccattta tacccatata
     8161 aatcagcatc catgttggaa tttaatcgcg gcctcgagca agacgtttcc cgttgaatat
     8221 ggctcataac accccttgta ttactgttta tgtaagcaga cagttttatt gttcatgatg
     8281 atatattttt atcttgtgca atgtaacatc agagattttg agacacaacg tggctttccc
     8341 ccccccccct gcaggtccga cacggggatg gatggcgttc ccgatcatgg tcctgcttgc
     8401 ttcgggtggc atcggaatgc cggcgctgca agcaatgttg tccaggcagg tggatgagga
     8461 acgtcagggg cagctgcaag gctcactggc ggcgctcacc agcctgacct cgatcgtcgg
     8521 acccctcctc ttcacggcga tctatgcggc ttctataaca acgtggaacg ggtgggcatg
     8581 gattgcaggc gctgccctct acttgctctg cctgccggcg ctgcgtcgcg ggctttggag
     8641 cggcgcaggg caacgagccg atcgctgatc gtggaaacga taggcctatg ccatgcgggt
     8701 caaggcgact tccggcaagc tatacgcgcc ctagaattgt caattttaat cctctgttta
     8761 tcggcagttc gtagagcgcg ccgtgcgtcc cgagcgatac tgagcgaagc aagtgcgtcg
     8821 agcagtgccc gcttgttcct gaaatgccag taaagcgctg gctgctgaac ccccagccgg
     8881 aactgacccc acaaggccct agcgtttgca atgcaccagg tcatcattga cccaggcgtg
     8941 ttccaccagg ccgctgcctc gcaactcttc gcaggcttcg ccgacctgct cgcgccactt
     9001 cttcacgcgg gtggaatccg atccgcacat gaggcggaag gtttccagct tgagcgggta
     9061 cggctcccgg tgcgagctga aatagtcgaa catccgtcgg gccgtcggcg acagcttgcg
     9121 gtacttctcc catatgaatt tcgtgtagtg gtcgccagca aacagcacga cgatttcctc
     9181 gtcgatcagg acctggcaac gggacgtttt cttgccacgg tccaggacgc ggaagcggtg
     9241 cagcagcgac accgattcca ggtgcccaac gcggtcggac gtgaagccca tcgccgtcgc
     9301 ctgtaggcgc gacaggcatt cctcggcctt cgtgtaatac cggccattga tcgaccagcc
     9361 caggtcctgg caaagctcgt agaacgtgaa ggtgatcggc tcgccgatag gggtgcgctt
     9421 cgcgtactcc aacacctgct gccacaccag ttcgtcatcg tcggcccgca gctcgacgcc
     9481 ggtgtaggtg atcttcacgt ccttgttgac gtggaaaatg accttgtttt gcagcgcctc
     9541 gcgcgggatt ttcttgttgc gcgtggtgaa cagggcagag cgggccgtgt cgtttggcat
     9601 cgctcgcatc gtgtccggcc acggcgcaat atcgaacaag gaaagctgca tttccttgat
     9661 ctgctgcttc gtgtgtttca gcaacgcggc ctgcttggcc tcgctgacct gttttgccag
     9721 gtcctcgccg gcggtttttc gcttcttggt cgtcatagtt cctcgcgtgt cgatggtcat
     9781 cgacttcgcc aaacctgccg cctcctgttc gagacgacgc gaacgctcca cggcggccga
     9841 tggcgcgggc agggcagggg gagccagttg cacgctgtcg cgctcgatct tggccgtagc
     9901 ttgctggacc atcgagccga cggactggaa ggtttcgcgg ggcgcacgca tgacggtgcg
     9961 gcttgcgatg gtttcggcat cctcggcgga aaaccccgcg tcgatcagtt cttgcctgta
    10021 tgccttccgg tcaaacgtcc gattcattca ccctccttgc gggattgccc cgactcacgc
    10081 cggggcaatg tgcccttatt cctgatttga cccgcctggt gccttggtgt ccagataatc
    10141 caccttatcg gcaatgaagt cggtcccgta gaccgtctgg ccgtccttct cgtacttggt
    10201 attccgaatc ttgccctgca cgaataccag ctccgcgaag tcgctcttct tgatggagcg
    10261 catggggacg tgcttggcaa tcacgcgcac cccccggccg ttttagcggc taaaaaagtc
    10321 atggctctgc cctcgggcgg accacgccca tcatgacctt gccaagctcg tcctgcttct
    10381 cttcgatctt cgccagcagg gcgaggatcg tggcatcacc gaaccgcgcc gtgcgcgggt
    10441 cgtcggtgag ccagagtttc agcaggccgc ccaggcggcc caggtcgcca ttgatgcggg
    10501 ccagctcgcg gacgtgctca tagtccacga cgcccgtgat tttgtagccc tggccgacgg
    10561 ccagcaggta ggcctacagg ctcatgccgg ccgccgccgc cttttcctca atcgctcttc
    10621 gttcgtctgg aaggcagtac accttgatag gtgggctgcc cttcctggtt ggcttggttt
    10681 catcagccat ccgcttgccc tcatctgtta cgccggcggt agccggccag cctcgcagag
    10741 caggattccc gttgagcacc gccaggtgcg aataagggac agtgaagaag gaacacccgc
    10801 tcgcgggtgg gcctacttca cctatcctgc ccggctgacg ccgttggata caccaaggaa
    10861 agtctacacg aaccctttgg caaaatcctg tatatcgtgc gaaaaaggat ggatataccg
    10921 aaaaaatcgc tataatgacc ccgaagcagg gttatgcagc ggaaaagatc cgtc
//
