LOCUS       40924_24263       10253 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pHC69, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10253)
  AUTHORS   Chou H-H., Berthet J, Marx CJ.
  TITLE     Growth rate modulates the selective advantage of recurrent 
            transposition mutations arising from experimental populations grown 
            in a metal-limited environment
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10253)
  AUTHORS   Chou H-H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2008) Organismic and Evolutionary Biology, Harvard
            University, 16 Divinity Avenue, Biolab 3079, Cambridge, MA 02138, 
            USA
REFERENCE   3  (bases 1 to 10253)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10253)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (15-OCT-2008) Organismic and Evolutionary Biology, Harvard 
            University, 16 Divinity Avenue, Biolab 3079, Cambridge, MA 02138, 
            USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10253
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      3..712
                     /label=oriV
                     /note="incP origin of replication"
     rep_origin      1105..1693
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    1981..2002
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2017..2047
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2055..2071
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2079..2095
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(2140..4254)
                     /codon_start=1
                     /gene="icuA"
                     /product="IcuA"
                     /label=icuA
                     /protein_id="ACR44965.1"
                     /translation="MSPLLTRLLASASALGVSTLPSHALDPTAVELEELQVTAQGGSAN
                     SVAAVAGPAANLKTQISPSVLALPGNSTVIDAAEIARLPVQSYGDLFRPVAGFDVSNYG
                     QGGVGYGIALRGFSDAEHGRDVAVFIDGVPINEVSSIHTPNYVDLNPLIPETIRSISIV
                     RGPFSVEAGDANLGGSIFIETKRAEPFSTVGASGGSFGTWRGLATFSQIGPGIQPFLAY
                     EGYGTGGYRENGDLGRYNAFNKVTIPLDLGSSLSLRVQMYGTGFGQPGYLERDRVRAGL
                     VSPRAATNITDGGGKTLQTFVANYVNGLPDDELSTLLYASHDVFSRFSDFGAGQRGQVE
                     ERETVGGRIRKVWTTSLFDVLPTQVLVGGDWRSDALDVNTGPSVARTFTARTLDLDLSQ
                     HNLAAYAQVQVKPAPWLKLTGGARFDQFFYDVRNNLDPALSPKVSPNVVSPKGGAALTP
                     FDWLELFANYGQGFRSPSASSELTNNPRLTPLTIESTEGGGRLVFDRFSLLGTVFFTDI
                     ANEVFQPAPGLPVQNLGKSRRDGFEIEAKVLAYREGLDRVALFANYSGVSARLLNGAPS
                     IFIPNVPAYLATVGAEFDVAAPAALLPGGRLTGAAYLSYIGAKPLTEDGVLHSSPYPRV
                     SGRIAYVHQSGWTTYGQATWYPGDRTSESIFNFGSSVSASAAEIVTSPQPRLTLLAGIT
                     SSFATGAPLR"
     gene            complement(2140..4254)
                     /gene="icuA"
                     /label=icuA
     promoter        complement(4282..4310)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     terminator      complement(4424..4451)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     terminator      complement(4543..4629)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     primer_bind     complement(4811..4827)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(5272..5913)
                     /codon_start=1
                     /label=TetR
                     /note="tetracycline resistance regulatory protein"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKG"
     CDS             complement(6694..7506)
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(8181..9326)
                     /codon_start=1
                     /label=trfA
                     /note="trans-acting replication protein that binds to and 
                     activates oriV"
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(9598..9969)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSL*AYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            complement(10002..10111)
                     /direction=LEFT
                     /label=oriT
                     /note="incP origin of transfer"
ORIGIN
        1 gaccctttcc gacgctcacc gggctggttg ccctcgccgc tgggctggcg gccgtctatg
       61 gccctgcaaa cgcgccagaa acgccgtcga agccgtgtgc gagacaccgc ggccgccggc
      121 gttgtggata cctcgcggaa aacttggccc tcactgacag atgaggggcg gacgttgaca
      181 cttgaggggc cgactcaccc ggcgcggcgt tgacagatga ggggcaggct cgatttcggc
      241 cggcgacgtg gagctggcca gcctcgcaaa tcggcgaaaa cgcctgattt tacgcgagtt
      301 tcccacagat gatgtggaca agcctgggga taagtgccct gcggtattga cacttgaggg
      361 gcgcgactac tgacagatga ggggcgcgat ccttgacact tgaggggcag agtgctgaca
      421 gatgaggggc gcacctattg acatttgagg ggctgtccac aggcagaaaa tccagcattt
      481 gcaagggttt ccgcccgttt ttcggccacc gctaacctgt cttttaacct gcttttaaac
      541 caatatttat aaaccttgtt tttaaccagg gctgcgccct gtgcgcgtga ccgcgcacgc
      601 cgaagggggg tgccccccct tctcgaaccc tcccggcccg ctaacgcggg cctcccatcc
      661 ccccaggggc tgcgcccctc ggccgcgaac ggcctcaccc caaaaatggc agccaagctg
      721 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
      781 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
      841 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
      901 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
      961 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     1021 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     1081 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     1141 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     1201 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt
     1261 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     1321 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     1381 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     1441 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     1501 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     1561 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     1621 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     1681 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     1741 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
     1801 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
     1861 cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca
     1921 ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat
     1981 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
     2041 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga
     2101 ttacgccaag cttgcatgcc taggctagca cggttcgtct catcgaagag gtgctcctgt
     2161 tgcaaagctg gaggtgatgc cggccagcag cgtcaggcgc ggctgcgggc tggtgacgat
     2221 ctcggcggcc gaggcagaga ccgacgagcc gaagttgaag atgctctcgc tcgtgcggtc
     2281 gccgggatac caggtggcct gcccgtaggt cgtccagccg ctctggtgga cgtaggcgat
     2341 ccggccgctg acgcgcggat agggcgagga atgcaatacg ccgtcctcgg tcaacggctt
     2401 ggcaccgatg taggacaggt aggccgcacc ggtcaggcgc ccgccgggca acagggcggc
     2461 cggcgcggcg acgtcgaatt cggcgccgac ggtggcaaga tacgccggca cgttggggat
     2521 gaagatggat ggcgcgccgt tgagcagtcg ggccgagacg cccgaatagt tggcgaacaa
     2581 cgccacgcgg tcgaggcctt cacgataggc cagcaccttc gcctcgattt cgaagccgtc
     2641 gcggcgggac ttgcccaggt tctgcacggg caggccgggt gcgggctgga agacctcgtt
     2701 ggcgatgtcg gtgaagaaca ccgtgccgag gagactgaac cggtcgaata cgaggcggcc
     2761 gccgccctcc gtactctcga tggtcagcgg agtgaggcgg ggattgttcg tcaattcgct
     2821 cgaagcggaa gggctgcgga agccctggcc gtaattggcg aacagctcca gccagtcgaa
     2881 gggcgtgagc gccgccccgc ccttggggga gaccacgttg gggctgacct tgggcgacag
     2941 ggccgggtcg agattgttgc gcacgtcgta gaagaactgg tcgaagcggg cgccgccggt
     3001 cagcttcagc cagggcgccg gcttgacctg cacctgcgcg taggccgcga ggttgtgctg
     3061 cgagagatcg agatcgaggg tgcgtgcggt gaacgtgcgc gcgaccgagg ggccggtgtt
     3121 cacgtcgagc gcgtcgctcc gccaatcgcc gccgacgagg acttgggtcg gcagcacgtc
     3181 gaacaggctc gtcgtccaga ccttgcggat gcggccaccc acggtctccc gttcctcgac
     3241 ctgtccgcgc tggccggcgc cgaaatccga gaagcggctg aacacgtcgt ggctggcgta
     3301 gagaagcgtc gacagctcgt cgtctggaag cccgttgacg tagttggcca cgaaggtctg
     3361 cagggtcttg ccgccgccat cggtgatgtt ggtcgcggcc cgcggtgaga cgagaccggc
     3421 gcgcacgcgg tcgcgctcca ggtagcccgg ctgcccgaac cccgtgccgt acatctgcac
     3481 ccgcagcgac aggctggagc cgagatcgag ggggatggtg accttgttga aggcgttgta
     3541 gcgcccgaga tcgccgttct cgcgatagcc cccggtgccg tagccctcgt aggcgaggaa
     3601 gggctggatc cccgggccga tctggctgaa ggtggcaagc ccccgccacg taccgaacga
     3661 gccgcccgat gcgccgacgg tcgagaaggg ctcggcccgc ttggtctcga tgaagatcga
     3721 gccgccgaga ttggcgtcgc ccgcttcgac cgagaacggg ccgcgcacga tgctgatgga
     3781 gcggatggtc tccgggatca gcggattgag atcgacgtag ttcggggtgt ggatcgagga
     3841 gacctcgttg atcggcaccc cgtcgatgaa aacggcgacg tcgcgaccgt gctcggcatc
     3901 gctgaagccg cgcagggcga tgccgtatcc gaccccgccc tgcccgtaat tcgagacatc
     3961 gaagcctgcc acgggacgga agaggtcgcc gtagctttgc accggcaggc gcgcgatctc
     4021 cgcggcatcg atcaccgtgc tgttgcccgg cagggccagg accgatggtg agatctgggt
     4081 cttcaggttc gcggccggtc ccgcaaccgc cgcaacggaa tttgcggaac cgccctgcgc
     4141 ggtgacctgt agttcctcca gctcaactgc ggtcggatcg agcgcatggc tcggaagcgt
     4201 actcacgccg agtgcgctgg ccgatgcgag caggcgcgtc agaagcggcg acattcgggg
     4261 tctctccctg gtacctccac acattatacg agccgatgat taattgtcaa cagctcattt
     4321 cagagaattc cctgctttcc tgatgcaaaa acgaggctag tttaccgtat ctgtgggggg
     4381 atggcttgta gatatgacga caggaagagt ttgtagaaac gcaaaaaggc catccgtcag
     4441 gatggccttc tgcttaattt gatgcctggc agtttatggc gggcgtcctg cccgccaccc
     4501 tccgggccgt tgcttcgcaa cgttcaaatc cgctcccggc ggatttgtcc tactcaggag
     4561 agcgttcacc gacaaacaac agataaaacg aaaggcccag tctttcgact gagcctttcg
     4621 ttttatttga tgcctggcag ttccctactc tcgcatgggg agaccccaca ctaccatcgg
     4681 cgctacggcg tttcacttct gagttcggca tggggtcagg tgggaccacc gcgctactgc
     4741 cgccaggcaa attctgtttt atcagaccgc ttctgcgttc tgatttaatc tgtatcaggc
     4801 tgaaaaattc actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc
     4861 aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc
     4921 gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggcgc ctgatgcggt
     4981 attttctcct tacgcatctg tgcggtattt cacaccgcat atggtgcact ctcagtacaa
     5041 tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc
     5101 cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga
     5161 gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacga aagggcctcg
     5221 tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag caccctttct
     5281 cggtccttca acgttcctga caacgagcct ccttttcgcc aatccatcga caatcaccgc
     5341 gagtccctgc tcgaacgctg cgtccggacc ggcttcgtcg aaggcgtcta tcgcggcccg
     5401 caacagcggc gagagcggag cctgttcaac ggtgccgccg cgctcgccgg catcgctgtc
     5461 gccggcctgc tcctcaagca cggccccaac agtgaagtag ctgattgtca tcagcgcatt
     5521 gacggcgtcc ccggccgaaa aacccgcctc gcagaggaag cgaagctgcg cgtcggccgt
     5581 ttccatctgc ggtgcgcccg gtcgcgtgcc ggcatggatg cgcgcgccat cgcggtaggc
     5641 gagcagcgcc tgcctgaagc tgcgggcatt cccgatcaga aatgagcgcc agtcgtcgtc
     5701 ggctctcggc accgaatgcg tatgattctc cgccagcatg gcttcggcca gtgcgtcgag
     5761 cagcgcccgc ttgttcctga agtgccagta aagcgccggc tgctgaaccc ccaaccgttc
     5821 cgccagtttg cgtgtcgtca gaccgtctac gccgacctcg ttcaacaggt ccagggcggc
     5881 acggatcact gtattcggct gcaactttgt catgattgac actttatcac tgataaacat
     5941 aatatgtcca ccaacttatc agtgataaag aatccgcgcg ttcaatcgga ccagcggagg
     6001 ctggtccgga ggccagacgt gaaacccaac atacccctga tcgtaattct gagcactgtc
     6061 gcgctcgacg ctgtcggcat cggcctgatt atgccggtgc tgccgggcct cctgcgcgat
     6121 ctggttcact cgaacgacgt caccgcccac tatggcattc tgctggcgct gtatgcgttg
     6181 gtgcaatttg cctgcgcacc tgtgctgggc gcgctgtcgg atcgtttcgg gcggcggcca
     6241 atcttgctcg tctcgctggc cggcgccact gtcgactacg ccatcatggc gacagcgcct
     6301 ttcctttggg ttctctatat cgggcggatc gtggccggca tcaccggggc gactggggcg
     6361 gtagccggcg cttatattgc cgatgacctg cagggggggg ggggcgctga ggtctgcctc
     6421 gtgaagaagg tgttgctgac tcataccagg cctgaatcgc cccatcatcc agccagaaag
     6481 tgagggagcc acggttgatg agagctttgt tgtaggtgga ccagttggtg attttgaact
     6541 tttgctttgc cacggaacgg tctgcgttgt cgggaagatg cgtgatctga tccttcaact
     6601 cagcaaaagt tcgatttatt caacaaagcc gccgtcccgt caagtcagcg taatgctctg
     6661 ccagtgttac aaccaattaa ccaattctga ttagaaaaac tcatcgagca tcaaatgaaa
     6721 ctgcaattta ttcatatcag gattatcaat accatatttt tgaaaaagcc gtttctgtaa
     6781 tgaaggagaa aactcaccga ggcagttcca taggatggca agatcctggt atcggtctgc
     6841 gattccgact cgtccaacat caatacaacc tattaatttc ccctcgtcaa aaataaggtt
     6901 atcaagtgag aaatcaccat gagtgacgac tgaatccggt gagaatggca aaagcttatg
     6961 catttctttc cagacttgtt caacaggcca gccattacgc tcgtcatcaa aatcactcgc
     7021 atcaaccaaa ccgttattca ttcgtgattg cgcctgagcg agacgaaata cgcgatcgct
     7081 gttaaaagga caattacaaa caggaatcga atgcaaccgg cgcaggaaca ctgccagcgc
     7141 atcaacaata ttttcacctg aatcaggata ttcttctaat acctggaatg ctgttttccc
     7201 ggggatcgca gtggtgagta accatgcatc atcaggagta cggataaaat gcttgatggt
     7261 cggaagaggc ataaattccg tcagccagtt tagtctgacc atctcatctg taacatcatt
     7321 ggcaacgcta cctttgccat gtttcagaaa caactctggc gcatcgggct tcccatacaa
     7381 tcgatagatt gtcgcacctg attgcccgac attatcgcga gcccatttat acccatataa
     7441 atcagcatcc atgttggaat ttaatcgcgg cctcgagcaa gacgtttccc gttgaatatg
     7501 gctcataaca ccccttgtat tactgtttat gtaagcagac agttttattg ttcatgatga
     7561 tatattttta tcttgtgcaa tgtaacatca gagattttga gacacaacgt ggctttcccc
     7621 cccccccctg caggtccgac acggggatgg atggcgttcc cgatcatggt cctgcttgct
     7681 tcgggtggca tcggaatgcc ggcgctgcaa gcaatgttgt ccaggcaggt ggatgaggaa
     7741 cgtcaggggc agctgcaagg ctcactggcg gcgctcacca gcctgacctc gatcgtcgga
     7801 cccctcctct tcacggcgat ctatgcggct tctataacaa cgtggaacgg gtgggcatgg
     7861 attgcaggcg ctgccctcta cttgctctgc ctgccggcgc tgcgtcgcgg gctttggagc
     7921 ggcgcagggc aacgagccga tcgctgatcg tggaaacgat aggcctatgc catgcgggtc
     7981 aaggcgactt ccggcaagct atacgcgccc tagaattgtc aattttaatc ctctgtttat
     8041 cggcagttcg tagagcgcgc cgtgcgtccc gagcgatact gagcgaagca agtgcgtcga
     8101 gcagtgcccg cttgttcctg aaatgccagt aaagcgctgg ctgctgaacc cccagccgga
     8161 actgacccca caaggcccta gcgtttgcaa tgcaccaggt catcattgac ccaggcgtgt
     8221 tccaccaggc cgctgcctcg caactcttcg caggcttcgc cgacctgctc gcgccacttc
     8281 ttcacgcggg tggaatccga tccgcacatg aggcggaagg tttccagctt gagcgggtac
     8341 ggctcccggt gcgagctgaa atagtcgaac atccgtcggg ccgtcggcga cagcttgcgg
     8401 tacttctccc atatgaattt cgtgtagtgg tcgccagcaa acagcacgac gatttcctcg
     8461 tcgatcagga cctggcaacg ggacgttttc ttgccacggt ccaggacgcg gaagcggtgc
     8521 agcagcgaca ccgattccag gtgcccaacg cggtcggacg tgaagcccat cgccgtcgcc
     8581 tgtaggcgcg acaggcattc ctcggccttc gtgtaatacc ggccattgat cgaccagccc
     8641 aggtcctggc aaagctcgta gaacgtgaag gtgatcggct cgccgatagg ggtgcgcttc
     8701 gcgtactcca acacctgctg ccacaccagt tcgtcatcgt cggcccgcag ctcgacgccg
     8761 gtgtaggtga tcttcacgtc cttgttgacg tggaaaatga ccttgttttg cagcgcctcg
     8821 cgcgggattt tcttgttgcg cgtggtgaac agggcagagc gggccgtgtc gtttggcatc
     8881 gctcgcatcg tgtccggcca cggcgcaata tcgaacaagg aaagctgcat ttccttgatc
     8941 tgctgcttcg tgtgtttcag caacgcggcc tgcttggcct cgctgacctg ttttgccagg
     9001 tcctcgccgg cggtttttcg cttcttggtc gtcatagttc ctcgcgtgtc gatggtcatc
     9061 gacttcgcca aacctgccgc ctcctgttcg agacgacgcg aacgctccac ggcggccgat
     9121 ggcgcgggca gggcaggggg agccagttgc acgctgtcgc gctcgatctt ggccgtagct
     9181 tgctggacca tcgagccgac ggactggaag gtttcgcggg gcgcacgcat gacggtgcgg
     9241 cttgcgatgg tttcggcatc ctcggcggaa aaccccgcgt cgatcagttc ttgcctgtat
     9301 gccttccggt caaacgtccg attcattcac cctccttgcg ggattgcccc gactcacgcc
     9361 ggggcaatgt gcccttattc ctgatttgac ccgcctggtg ccttggtgtc cagataatcc
     9421 accttatcgg caatgaagtc ggtcccgtag accgtctggc cgtccttctc gtacttggta
     9481 ttccgaatct tgccctgcac gaataccagc tccgcgaagt cgctcttctt gatggagcgc
     9541 atggggacgt gcttggcaat cacgcgcacc ccccggccgt tttagcggct aaaaaagtca
     9601 tggctctgcc ctcgggcgga ccacgcccat catgaccttg ccaagctcgt cctgcttctc
     9661 ttcgatcttc gccagcaggg cgaggatcgt ggcatcaccg aaccgcgccg tgcgcgggtc
     9721 gtcggtgagc cagagtttca gcaggccgcc caggcggccc aggtcgccat tgatgcgggc
     9781 cagctcgcgg acgtgctcat agtccacgac gcccgtgatt ttgtagccct ggccgacggc
     9841 cagcaggtag gcctacaggc tcatgccggc cgccgccgcc ttttcctcaa tcgctcttcg
     9901 ttcgtctgga aggcagtaca ccttgatagg tgggctgccc ttcctggttg gcttggtttc
     9961 atcagccatc cgcttgccct catctgttac gccggcggta gccggccagc ctcgcagagc
    10021 aggattcccg ttgagcaccg ccaggtgcga ataagggaca gtgaagaagg aacacccgct
    10081 cgcgggtggg cctacttcac ctatcctgcc cggctgacgc cgttggatac accaaggaaa
    10141 gtctacacga accctttggc aaaatcctgt atatcgtgcg aaaaaggatg gatataccga
    10201 aaaaatcgct ataatgaccc cgaagcaggg ttatgcagcg gaaaagatcc gtc
//
