LOCUS       40924_24228       10586 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pHC51, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10586)
  AUTHORS   Chou H-H., Berthet J, Marx CJ.
  TITLE     Growth rate modulates the selective advantage of recurrent 
            transposition mutations arising from experimental populations grown 
            in a metal-limited environment
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10586)
  AUTHORS   Chou H-H.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2008) Organismic and Evolutionary Biology, Harvard
            University, 16 Divinity Avenue, Biolab 3079, Cambridge, MA 02138, 
            USA
REFERENCE   3  (bases 1 to 10586)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10586)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (14-OCT-2008) Organismic and Evolutionary Biology, Harvard 
            University, 16 Divinity Avenue, Biolab 3079, Cambridge, MA 02138, 
            USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10586
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      3..712
                     /label=oriV
                     /note="incP origin of replication"
     rep_origin      1105..1693
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    1981..2002
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2017..2047
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2055..2071
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2079..2095
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(2123..2836)
                     /codon_start=1
                     /label=GFPuv
                     /note="GFP variant optimized for excitation by UV light"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     misc_feature    complement(3039..4657)
                     /label=5' upstream region of icuAB.1145
                     /note="5' upstream region of icuAB.1145"
     terminator      complement(4757..4784)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     terminator      complement(4876..4962)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     primer_bind     complement(5144..5160)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(5605..6246)
                     /codon_start=1
                     /label=TetR
                     /note="tetracycline resistance regulatory protein"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKG"
     CDS             complement(7027..7839)
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(8514..9659)
                     /codon_start=1
                     /label=trfA
                     /note="trans-acting replication protein that binds to and 
                     activates oriV"
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(9931..10302)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSL*AYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            complement(10335..10444)
                     /direction=LEFT
                     /label=oriT
                     /note="incP origin of transfer"
ORIGIN
        1 gaccctttcc gacgctcacc gggctggttg ccctcgccgc tgggctggcg gccgtctatg
       61 gccctgcaaa cgcgccagaa acgccgtcga agccgtgtgc gagacaccgc ggccgccggc
      121 gttgtggata cctcgcggaa aacttggccc tcactgacag atgaggggcg gacgttgaca
      181 cttgaggggc cgactcaccc ggcgcggcgt tgacagatga ggggcaggct cgatttcggc
      241 cggcgacgtg gagctggcca gcctcgcaaa tcggcgaaaa cgcctgattt tacgcgagtt
      301 tcccacagat gatgtggaca agcctgggga taagtgccct gcggtattga cacttgaggg
      361 gcgcgactac tgacagatga ggggcgcgat ccttgacact tgaggggcag agtgctgaca
      421 gatgaggggc gcacctattg acatttgagg ggctgtccac aggcagaaaa tccagcattt
      481 gcaagggttt ccgcccgttt ttcggccacc gctaacctgt cttttaacct gcttttaaac
      541 caatatttat aaaccttgtt tttaaccagg gctgcgccct gtgcgcgtga ccgcgcacgc
      601 cgaagggggg tgccccccct tctcgaaccc tcccggcccg ctaacgcggg cctcccatcc
      661 ccccaggggc tgcgcccctc ggccgcgaac ggcctcaccc caaaaatggc agccaagctg
      721 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
      781 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
      841 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
      901 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
      961 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     1021 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     1081 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     1141 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     1201 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt
     1261 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     1321 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     1381 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     1441 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     1501 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     1561 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     1621 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     1681 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     1741 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
     1801 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
     1861 cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca
     1921 ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat
     1981 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
     2041 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga
     2101 ttacgccaag cttgcatgct tatttgtaga gctcatccat gccatgtgta atcccagcag
     2161 cagttacaaa ctcaagaagg accatgtggt cacgcttttc gttgggatct ttcgaaaggg
     2221 cagattgtgt cgacaggtaa tggttgtctg gtaaaaggac agggccatcg ccaattggag
     2281 tattttgttg ataatggtct gctagttgaa cggatccatc ttcaatgttg tggcgaattt
     2341 tgaagttagc tttgattcca ttcttttgtt tgtctgccgt gatgtataca ttgtgtgagt
     2401 tatagttgta ctcgagtttg tgtccgagaa tgtttccatc ttctttaaaa tcaatacctt
     2461 ttaactcgat acgattaaca agggtatcac cttcaaactt gacttcagca cgcgtcttgt
     2521 agttcccgtc atctttgaaa gatatagtgc gttcctgtac ataaccttcg ggcatggcac
     2581 tcttgaaaaa gtcatgccgt ttcatatgat ccggataacg ggaaaagcat tgaacaccat
     2641 aagagaaagt agtgacaagt gttggccatg gaacaggtag ttttccagta gtgcaaataa
     2701 atttaagggt aagttttccg tatgttgcat caccttcacc ctctccactg acagaaaatt
     2761 tgtgcccatt aacatcacca tctaattcaa caagaattgg gacaactcca gtgaaaagtt
     2821 cttctccttt actcattcgg ggtctctccc tgctagcggg cccggatccg atatcttaag
     2881 atcttccgtg cagtttaagc cgaattgatc cccgggtacc cgtgcaacct aggcactcag
     2941 tgatccgggc gctactaaac tgcaaattat gggcaggcac aagataggaa tttttcgtat
     3001 tcgtacgtct catcgagatg ttgcagcctt tagcccgtct tattcacgag ggcagtggat
     3061 ctgtggtggc gagcgtagcg taaagcattg atgtggctta gggattgggt gctgacgcca
     3121 agtcctcaga ccagatcgag cccgccacgc ccgccatgtc cgatcctacg tgtctgccgt
     3181 tcgcgtttcc atcggttcga ggcaagaagc tcacagctgc tttcgacggc ggacgcctga
     3241 cctcggacgg gggcgtcctg ttgctcgctc aggctgcccg gcggatgcag atcggtgaga
     3301 agctcgccgc ggtgatcccc gaccggcgtg acccgagccg gatcgtgcat cctctgcccg
     3361 agatcctgct ggcgcgcatc ctggccatcg cctgcgggta cgaggatgcc gacgacctcg
     3421 atcacctgcg cgtcgatccc gccttcaagc tcgcctgtgg tcgcctaccc gacagcggac
     3481 gcgacttgat gagccagccc accgtctcgc ggctggagaa cacgccgggc ctgcgtgacc
     3541 tgatccggct cggacgggtg ctggtcgacc tctactgcgc cagctacgcc gcgccaccgg
     3601 cggctgtcac gctggatatc gacgacacct gcgacgtggt ccatggccag cagcaactgt
     3661 cgctgttcaa cgcccaccac gacgaacgct gctttctccc catccacgtc tacgacaccg
     3721 ccacctcgcg cccggtggcg atgatcctgc ggccgggcaa gacgccttcg ggtcgcgaag
     3781 tccggggcca tctgcgtcgt ctggtccggg cgatccgccg acactggccg acgaccgcga
     3841 tcacgatccg tggcgacggc cattacgggc ggcccgaagc gatggactgg tgcgaggaca
     3901 acgacgtcgc ttacgtcttc ggcctgaccg gcaccaagtc gctcaccgcc aaggtcgagc
     3961 cgaccgccga tcacatccgg gtcgagcggg ccctcagcaa cacagatgcc gtgcgcggct
     4021 ttgctgagac cacgcacgca gccaaatcct ggcggcagca ccgccgcgtc gccgcccgca
     4081 tcgaggcgac ccgccttggc ctcgacatcc gctacgtggt cacgaacatc gccaccggta
     4141 cgcccgaatg gctctacgcc gagctgtact gcgcccgcgg acaggccgag aacctgatca
     4201 agctccataa gggccagctc gcttccgacc gcacctcctg ccggcatccg gccgccaacc
     4261 agatgcggct cgtgctgcac accgccgcct attggctgat gctgaccgtg cgcgacgcca
     4321 tcccggctcc ccatcgcctg gccaaggccg agttcgcgac gatccgcctg cgcctgctca
     4381 agcttggggc tcgcatccac gagaccactg cccgtgttcg tctcgccttc gccgccgcct
     4441 gtcccgaggc cgacttgctg cgccatatcg cgggcgcact cgccccagcg ccagcctgaa
     4501 ccacggggcg tgatgcccca tcgacaccgc tcccgtccct ccagcgccga cagcacaacc
     4561 agatccatac gcgtcaggac agacgcacga tcccgctcgc cttcagctca aaacacgtcg
     4621 ccagccaaac cctggtgaat aaagcgggtt agcagccgaa ttccctgctt tcctgatgca
     4681 aaaacgaggc tagtttaccg tatctgtggg gggatggctt gtagatatga cgacaggaag
     4741 agtttgtaga aacgcaaaaa ggccatccgt caggatggcc ttctgcttaa tttgatgcct
     4801 ggcagtttat ggcgggcgtc ctgcccgcca ccctccgggc cgttgcttcg caacgttcaa
     4861 atccgctccc ggcggatttg tcctactcag gagagcgttc accgacaaac aacagataaa
     4921 acgaaaggcc cagtctttcg actgagcctt tcgttttatt tgatgcctgg cagttcccta
     4981 ctctcgcatg gggagacccc acactaccat cggcgctacg gcgtttcact tctgagttcg
     5041 gcatggggtc aggtgggacc accgcgctac tgccgccagg caaattctgt tttatcagac
     5101 cgcttctgcg ttctgattta atctgtatca ggctgaaaaa ttcactggcc gtcgttttac
     5161 aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc
     5221 ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc
     5281 gcagcctgaa tggcgaatgg cgcctgatgc ggtattttct ccttacgcat ctgtgcggta
     5341 tttcacaccg catatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc
     5401 agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat
     5461 ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt
     5521 catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg cctattttta taggttaatg
     5581 tcatgataat aatggtttct tagcaccctt tctcggtcct tcaacgttcc tgacaacgag
     5641 cctccttttc gccaatccat cgacaatcac cgcgagtccc tgctcgaacg ctgcgtccgg
     5701 accggcttcg tcgaaggcgt ctatcgcggc ccgcaacagc ggcgagagcg gagcctgttc
     5761 aacggtgccg ccgcgctcgc cggcatcgct gtcgccggcc tgctcctcaa gcacggcccc
     5821 aacagtgaag tagctgattg tcatcagcgc attgacggcg tccccggccg aaaaacccgc
     5881 ctcgcagagg aagcgaagct gcgcgtcggc cgtttccatc tgcggtgcgc ccggtcgcgt
     5941 gccggcatgg atgcgcgcgc catcgcggta ggcgagcagc gcctgcctga agctgcgggc
     6001 attcccgatc agaaatgagc gccagtcgtc gtcggctctc ggcaccgaat gcgtatgatt
     6061 ctccgccagc atggcttcgg ccagtgcgtc gagcagcgcc cgcttgttcc tgaagtgcca
     6121 gtaaagcgcc ggctgctgaa cccccaaccg ttccgccagt ttgcgtgtcg tcagaccgtc
     6181 tacgccgacc tcgttcaaca ggtccagggc ggcacggatc actgtattcg gctgcaactt
     6241 tgtcatgatt gacactttat cactgataaa cataatatgt ccaccaactt atcagtgata
     6301 aagaatccgc gcgttcaatc ggaccagcgg aggctggtcc ggaggccaga cgtgaaaccc
     6361 aacatacccc tgatcgtaat tctgagcact gtcgcgctcg acgctgtcgg catcggcctg
     6421 attatgccgg tgctgccggg cctcctgcgc gatctggttc actcgaacga cgtcaccgcc
     6481 cactatggca ttctgctggc gctgtatgcg ttggtgcaat ttgcctgcgc acctgtgctg
     6541 ggcgcgctgt cggatcgttt cgggcggcgg ccaatcttgc tcgtctcgct ggccggcgcc
     6601 actgtcgact acgccatcat ggcgacagcg cctttccttt gggttctcta tatcgggcgg
     6661 atcgtggccg gcatcaccgg ggcgactggg gcggtagccg gcgcttatat tgccgatgac
     6721 ctgcaggggg gggggggcgc tgaggtctgc ctcgtgaaga aggtgttgct gactcatacc
     6781 aggcctgaat cgccccatca tccagccaga aagtgaggga gccacggttg atgagagctt
     6841 tgttgtaggt ggaccagttg gtgattttga acttttgctt tgccacggaa cggtctgcgt
     6901 tgtcgggaag atgcgtgatc tgatccttca actcagcaaa agttcgattt attcaacaaa
     6961 gccgccgtcc cgtcaagtca gcgtaatgct ctgccagtgt tacaaccaat taaccaattc
     7021 tgattagaaa aactcatcga gcatcaaatg aaactgcaat ttattcatat caggattatc
     7081 aataccatat ttttgaaaaa gccgtttctg taatgaagga gaaaactcac cgaggcagtt
     7141 ccataggatg gcaagatcct ggtatcggtc tgcgattccg actcgtccaa catcaataca
     7201 acctattaat ttcccctcgt caaaaataag gttatcaagt gagaaatcac catgagtgac
     7261 gactgaatcc ggtgagaatg gcaaaagctt atgcatttct ttccagactt gttcaacagg
     7321 ccagccatta cgctcgtcat caaaatcact cgcatcaacc aaaccgttat tcattcgtga
     7381 ttgcgcctga gcgagacgaa atacgcgatc gctgttaaaa ggacaattac aaacaggaat
     7441 cgaatgcaac cggcgcagga acactgccag cgcatcaaca atattttcac ctgaatcagg
     7501 atattcttct aatacctgga atgctgtttt cccggggatc gcagtggtga gtaaccatgc
     7561 atcatcagga gtacggataa aatgcttgat ggtcggaaga ggcataaatt ccgtcagcca
     7621 gtttagtctg accatctcat ctgtaacatc attggcaacg ctacctttgc catgtttcag
     7681 aaacaactct ggcgcatcgg gcttcccata caatcgatag attgtcgcac ctgattgccc
     7741 gacattatcg cgagcccatt tatacccata taaatcagca tccatgttgg aatttaatcg
     7801 cggcctcgag caagacgttt cccgttgaat atggctcata acaccccttg tattactgtt
     7861 tatgtaagca gacagtttta ttgttcatga tgatatattt ttatcttgtg caatgtaaca
     7921 tcagagattt tgagacacaa cgtggctttc cccccccccc ctgcaggtcc gacacgggga
     7981 tggatggcgt tcccgatcat ggtcctgctt gcttcgggtg gcatcggaat gccggcgctg
     8041 caagcaatgt tgtccaggca ggtggatgag gaacgtcagg ggcagctgca aggctcactg
     8101 gcggcgctca ccagcctgac ctcgatcgtc ggacccctcc tcttcacggc gatctatgcg
     8161 gcttctataa caacgtggaa cgggtgggca tggattgcag gcgctgccct ctacttgctc
     8221 tgcctgccgg cgctgcgtcg cgggctttgg agcggcgcag ggcaacgagc cgatcgctga
     8281 tcgtggaaac gataggccta tgccatgcgg gtcaaggcga cttccggcaa gctatacgcg
     8341 ccctagaatt gtcaatttta atcctctgtt tatcggcagt tcgtagagcg cgccgtgcgt
     8401 cccgagcgat actgagcgaa gcaagtgcgt cgagcagtgc ccgcttgttc ctgaaatgcc
     8461 agtaaagcgc tggctgctga acccccagcc ggaactgacc ccacaaggcc ctagcgtttg
     8521 caatgcacca ggtcatcatt gacccaggcg tgttccacca ggccgctgcc tcgcaactct
     8581 tcgcaggctt cgccgacctg ctcgcgccac ttcttcacgc gggtggaatc cgatccgcac
     8641 atgaggcgga aggtttccag cttgagcggg tacggctccc ggtgcgagct gaaatagtcg
     8701 aacatccgtc gggccgtcgg cgacagcttg cggtacttct cccatatgaa tttcgtgtag
     8761 tggtcgccag caaacagcac gacgatttcc tcgtcgatca ggacctggca acgggacgtt
     8821 ttcttgccac ggtccaggac gcggaagcgg tgcagcagcg acaccgattc caggtgccca
     8881 acgcggtcgg acgtgaagcc catcgccgtc gcctgtaggc gcgacaggca ttcctcggcc
     8941 ttcgtgtaat accggccatt gatcgaccag cccaggtcct ggcaaagctc gtagaacgtg
     9001 aaggtgatcg gctcgccgat aggggtgcgc ttcgcgtact ccaacacctg ctgccacacc
     9061 agttcgtcat cgtcggcccg cagctcgacg ccggtgtagg tgatcttcac gtccttgttg
     9121 acgtggaaaa tgaccttgtt ttgcagcgcc tcgcgcggga ttttcttgtt gcgcgtggtg
     9181 aacagggcag agcgggccgt gtcgtttggc atcgctcgca tcgtgtccgg ccacggcgca
     9241 atatcgaaca aggaaagctg catttccttg atctgctgct tcgtgtgttt cagcaacgcg
     9301 gcctgcttgg cctcgctgac ctgttttgcc aggtcctcgc cggcggtttt tcgcttcttg
     9361 gtcgtcatag ttcctcgcgt gtcgatggtc atcgacttcg ccaaacctgc cgcctcctgt
     9421 tcgagacgac gcgaacgctc cacggcggcc gatggcgcgg gcagggcagg gggagccagt
     9481 tgcacgctgt cgcgctcgat cttggccgta gcttgctgga ccatcgagcc gacggactgg
     9541 aaggtttcgc ggggcgcacg catgacggtg cggcttgcga tggtttcggc atcctcggcg
     9601 gaaaaccccg cgtcgatcag ttcttgcctg tatgccttcc ggtcaaacgt ccgattcatt
     9661 caccctcctt gcgggattgc cccgactcac gccggggcaa tgtgccctta ttcctgattt
     9721 gacccgcctg gtgccttggt gtccagataa tccaccttat cggcaatgaa gtcggtcccg
     9781 tagaccgtct ggccgtcctt ctcgtacttg gtattccgaa tcttgccctg cacgaatacc
     9841 agctccgcga agtcgctctt cttgatggag cgcatgggga cgtgcttggc aatcacgcgc
     9901 accccccggc cgttttagcg gctaaaaaag tcatggctct gccctcgggc ggaccacgcc
     9961 catcatgacc ttgccaagct cgtcctgctt ctcttcgatc ttcgccagca gggcgaggat
    10021 cgtggcatca ccgaaccgcg ccgtgcgcgg gtcgtcggtg agccagagtt tcagcaggcc
    10081 gcccaggcgg cccaggtcgc cattgatgcg ggccagctcg cggacgtgct catagtccac
    10141 gacgcccgtg attttgtagc cctggccgac ggccagcagg taggcctaca ggctcatgcc
    10201 ggccgccgcc gccttttcct caatcgctct tcgttcgtct ggaaggcagt acaccttgat
    10261 aggtgggctg cccttcctgg ttggcttggt ttcatcagcc atccgcttgc cctcatctgt
    10321 tacgccggcg gtagccggcc agcctcgcag agcaggattc ccgttgagca ccgccaggtg
    10381 cgaataaggg acagtgaaga aggaacaccc gctcgcgggt gggcctactt cacctatcct
    10441 gcccggctga cgccgttgga tacaccaagg aaagtctaca cgaacccttt ggcaaaatcc
    10501 tgtatatcgt gcgaaaaagg atggatatac cgaaaaaatc gctataatga ccccgaagca
    10561 gggttatgca gcggaaaaga tccgtc
//
