LOCUS       40924_24223       10467 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pHC47, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10467)
  AUTHORS   Chou H-H., Berthet J, Marx CJ.
  TITLE     Growth rate modulates the selective advantage of recurrent 
            transposition mutations arising from experimental populations grown 
            in a metal-limited environment
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10467)
  AUTHORS   Chou H-H.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2008) Organismic and Evolutionary Biology, Harvard
            University, 16 Divinity Avenue, Biolab 3079, Cambridge, MA 02138, 
            USA
REFERENCE   3  (bases 1 to 10467)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10467)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (14-OCT-2008) Organismic and Evolutionary Biology, Harvard 
            University, 16 Divinity Avenue, Biolab 3079, Cambridge, MA 02138, 
            USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10467
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      3..712
                     /label=oriV
                     /note="incP origin of replication"
     rep_origin      1105..1693
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    1981..2002
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2017..2047
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2055..2071
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2079..2095
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(2123..2836)
                     /codon_start=1
                     /label=GFPuv
                     /note="GFP variant optimized for excitation by UV light"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     misc_feature    complement(2920..4538)
                     /label=ISMex4
                     /note="ISMex4"
     terminator      complement(4638..4665)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     terminator      complement(4757..4843)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     primer_bind     complement(5025..5041)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(5486..6127)
                     /codon_start=1
                     /label=TetR
                     /note="tetracycline resistance regulatory protein"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKG"
     CDS             complement(6908..7720)
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(8395..9540)
                     /codon_start=1
                     /label=trfA
                     /note="trans-acting replication protein that binds to and 
                     activates oriV"
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(9812..10183)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSL*AYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            complement(10216..10325)
                     /direction=LEFT
                     /label=oriT
                     /note="incP origin of transfer"
ORIGIN
        1 gaccctttcc gacgctcacc gggctggttg ccctcgccgc tgggctggcg gccgtctatg
       61 gccctgcaaa cgcgccagaa acgccgtcga agccgtgtgc gagacaccgc ggccgccggc
      121 gttgtggata cctcgcggaa aacttggccc tcactgacag atgaggggcg gacgttgaca
      181 cttgaggggc cgactcaccc ggcgcggcgt tgacagatga ggggcaggct cgatttcggc
      241 cggcgacgtg gagctggcca gcctcgcaaa tcggcgaaaa cgcctgattt tacgcgagtt
      301 tcccacagat gatgtggaca agcctgggga taagtgccct gcggtattga cacttgaggg
      361 gcgcgactac tgacagatga ggggcgcgat ccttgacact tgaggggcag agtgctgaca
      421 gatgaggggc gcacctattg acatttgagg ggctgtccac aggcagaaaa tccagcattt
      481 gcaagggttt ccgcccgttt ttcggccacc gctaacctgt cttttaacct gcttttaaac
      541 caatatttat aaaccttgtt tttaaccagg gctgcgccct gtgcgcgtga ccgcgcacgc
      601 cgaagggggg tgccccccct tctcgaaccc tcccggcccg ctaacgcggg cctcccatcc
      661 ccccaggggc tgcgcccctc ggccgcgaac ggcctcaccc caaaaatggc agccaagctg
      721 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
      781 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
      841 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
      901 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
      961 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     1021 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     1081 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     1141 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     1201 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt
     1261 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     1321 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     1381 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     1441 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     1501 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     1561 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     1621 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     1681 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     1741 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
     1801 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
     1861 cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca
     1921 ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat
     1981 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
     2041 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga
     2101 ttacgccaag cttgcatgct tatttgtaga gctcatccat gccatgtgta atcccagcag
     2161 cagttacaaa ctcaagaagg accatgtggt cacgcttttc gttgggatct ttcgaaaggg
     2221 cagattgtgt cgacaggtaa tggttgtctg gtaaaaggac agggccatcg ccaattggag
     2281 tattttgttg ataatggtct gctagttgaa cggatccatc ttcaatgttg tggcgaattt
     2341 tgaagttagc tttgattcca ttcttttgtt tgtctgccgt gatgtataca ttgtgtgagt
     2401 tatagttgta ctcgagtttg tgtccgagaa tgtttccatc ttctttaaaa tcaatacctt
     2461 ttaactcgat acgattaaca agggtatcac cttcaaactt gacttcagca cgcgtcttgt
     2521 agttcccgtc atctttgaaa gatatagtgc gttcctgtac ataaccttcg ggcatggcac
     2581 tcttgaaaaa gtcatgccgt ttcatatgat ccggataacg ggaaaagcat tgaacaccat
     2641 aagagaaagt agtgacaagt gttggccatg gaacaggtag ttttccagta gtgcaaataa
     2701 atttaagggt aagttttccg tatgttgcat caccttcacc ctctccactg acagaaaatt
     2761 tgtgcccatt aacatcacca tctaattcaa caagaattgg gacaactcca gtgaaaagtt
     2821 cttctccttt actcattcgg ggtctctccc tgctagcggg cccggatccg atatcttaag
     2881 atcttccgtg cagtttaagc cgaattgatc cccgggtacc ttattcacga gggcagtgga
     2941 tctgtggtgg cgagcgtagc gtaaagcatt gatgtggctt agggattggg tgctgacgcc
     3001 aagtcctcag accagatcga gcccgccacg cccgccatgt ccgatcctac gtgtctgccg
     3061 ttcgcgtttc catcggttcg aggcaagaag ctcacagctg ctttcgacgg cggacgcctg
     3121 acctcggacg ggggcgtcct gttgctcgct caggctgccc ggcggatgca gatcggtgag
     3181 aagctcgccg cggtgatccc cgaccggcgt gacccgagcc ggatcgtgca tcctctgccc
     3241 gagatcctgc tggcgcgcat cctggccatc gcctgcgggt acgaggatgc cgacgacctc
     3301 gatcacctgc gcgtcgatcc cgccttcaag ctcgcctgtg gtcgcctacc cgacagcgga
     3361 cgcgacttga tgagccagcc caccgtctcg cggctggaga acacgccggg cctgcgtgac
     3421 ctgatccggc tcggacgggt gctggtcgac ctctactgcg ccagctacgc cgcgccaccg
     3481 gcggctgtca cgctggatat cgacgacacc tgcgacgtgg tccatggcca gcagcaactg
     3541 tcgctgttca acgcccacca cgacgaacgc tgctttctcc ccatccacgt ctacgacacc
     3601 gccacctcgc gcccggtggc gatgatcctg cggccgggca agacgccttc gggtcgcgaa
     3661 gtccggggcc atctgcgtcg tctggtccgg gcgatccgcc gacactggcc gacgaccgcg
     3721 atcacgatcc gtggcgacgg ccattacggg cggcccgaag cgatggactg gtgcgaggac
     3781 aacgacgtcg cttacgtctt cggcctgacc ggcaccaagt cgctcaccgc caaggtcgag
     3841 ccgaccgccg atcacatccg ggtcgagcgg gccctcagca acacagatgc cgtgcgcggc
     3901 tttgctgaga ccacgcacgc agccaaatcc tggcggcagc accgccgcgt cgccgcccgc
     3961 atcgaggcga cccgccttgg cctcgacatc cgctacgtgg tcacgaacat cgccaccggt
     4021 acgcccgaat ggctctacgc cgagctgtac tgcgcccgcg gacaggccga gaacctgatc
     4081 aagctccata agggccagct cgcttccgac cgcacctcct gccggcatcc ggccgccaac
     4141 cagatgcggc tcgtgctgca caccgccgcc tattggctga tgctgaccgt gcgcgacgcc
     4201 atcccggctc cccatcgcct ggccaaggcc gagttcgcga cgatccgcct gcgcctgctc
     4261 aagcttgggg ctcgcatcca cgagaccact gcccgtgttc gtctcgcctt cgccgccgcc
     4321 tgtcccgagg ccgacttgct gcgccatatc gcgggcgcac tcgccccagc gccagcctga
     4381 accacggggc gtgatgcccc atcgacaccg ctcccgtccc tccagcgccg acagcacaac
     4441 cagatccata cgcgtcagga cagacgcacg atcccgctcg ccttcagctc aaaacacgtc
     4501 gccagccaaa ccctggtgaa taaagcgggt tagcagccga attccctgct ttcctgatgc
     4561 aaaaacgagg ctagtttacc gtatctgtgg ggggatggct tgtagatatg acgacaggaa
     4621 gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta atttgatgcc
     4681 tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc gcaacgttca
     4741 aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa
     4801 aacgaaaggc ccagtctttc gactgagcct ttcgttttat ttgatgcctg gcagttccct
     4861 actctcgcat ggggagaccc cacactacca tcggcgctac ggcgtttcac ttctgagttc
     4921 ggcatggggt caggtgggac caccgcgcta ctgccgccag gcaaattctg ttttatcaga
     4981 ccgcttctgc gttctgattt aatctgtatc aggctgaaaa attcactggc cgtcgtttta
     5041 caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc
     5101 cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg
     5161 cgcagcctga atggcgaatg gcgcctgatg cggtattttc tccttacgca tctgtgcggt
     5221 atttcacacc gcatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc
     5281 cagccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca
     5341 tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg
     5401 tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac gcctattttt ataggttaat
     5461 gtcatgataa taatggtttc ttagcaccct ttctcggtcc ttcaacgttc ctgacaacga
     5521 gcctcctttt cgccaatcca tcgacaatca ccgcgagtcc ctgctcgaac gctgcgtccg
     5581 gaccggcttc gtcgaaggcg tctatcgcgg cccgcaacag cggcgagagc ggagcctgtt
     5641 caacggtgcc gccgcgctcg ccggcatcgc tgtcgccggc ctgctcctca agcacggccc
     5701 caacagtgaa gtagctgatt gtcatcagcg cattgacggc gtccccggcc gaaaaacccg
     5761 cctcgcagag gaagcgaagc tgcgcgtcgg ccgtttccat ctgcggtgcg cccggtcgcg
     5821 tgccggcatg gatgcgcgcg ccatcgcggt aggcgagcag cgcctgcctg aagctgcggg
     5881 cattcccgat cagaaatgag cgccagtcgt cgtcggctct cggcaccgaa tgcgtatgat
     5941 tctccgccag catggcttcg gccagtgcgt cgagcagcgc ccgcttgttc ctgaagtgcc
     6001 agtaaagcgc cggctgctga acccccaacc gttccgccag tttgcgtgtc gtcagaccgt
     6061 ctacgccgac ctcgttcaac aggtccaggg cggcacggat cactgtattc ggctgcaact
     6121 ttgtcatgat tgacacttta tcactgataa acataatatg tccaccaact tatcagtgat
     6181 aaagaatccg cgcgttcaat cggaccagcg gaggctggtc cggaggccag acgtgaaacc
     6241 caacataccc ctgatcgtaa ttctgagcac tgtcgcgctc gacgctgtcg gcatcggcct
     6301 gattatgccg gtgctgccgg gcctcctgcg cgatctggtt cactcgaacg acgtcaccgc
     6361 ccactatggc attctgctgg cgctgtatgc gttggtgcaa tttgcctgcg cacctgtgct
     6421 gggcgcgctg tcggatcgtt tcgggcggcg gccaatcttg ctcgtctcgc tggccggcgc
     6481 cactgtcgac tacgccatca tggcgacagc gcctttcctt tgggttctct atatcgggcg
     6541 gatcgtggcc ggcatcaccg gggcgactgg ggcggtagcc ggcgcttata ttgccgatga
     6601 cctgcagggg ggggggggcg ctgaggtctg cctcgtgaag aaggtgttgc tgactcatac
     6661 caggcctgaa tcgccccatc atccagccag aaagtgaggg agccacggtt gatgagagct
     6721 ttgttgtagg tggaccagtt ggtgattttg aacttttgct ttgccacgga acggtctgcg
     6781 ttgtcgggaa gatgcgtgat ctgatccttc aactcagcaa aagttcgatt tattcaacaa
     6841 agccgccgtc ccgtcaagtc agcgtaatgc tctgccagtg ttacaaccaa ttaaccaatt
     6901 ctgattagaa aaactcatcg agcatcaaat gaaactgcaa tttattcata tcaggattat
     6961 caataccata tttttgaaaa agccgtttct gtaatgaagg agaaaactca ccgaggcagt
     7021 tccataggat ggcaagatcc tggtatcggt ctgcgattcc gactcgtcca acatcaatac
     7081 aacctattaa tttcccctcg tcaaaaataa ggttatcaag tgagaaatca ccatgagtga
     7141 cgactgaatc cggtgagaat ggcaaaagct tatgcatttc tttccagact tgttcaacag
     7201 gccagccatt acgctcgtca tcaaaatcac tcgcatcaac caaaccgtta ttcattcgtg
     7261 attgcgcctg agcgagacga aatacgcgat cgctgttaaa aggacaatta caaacaggaa
     7321 tcgaatgcaa ccggcgcagg aacactgcca gcgcatcaac aatattttca cctgaatcag
     7381 gatattcttc taatacctgg aatgctgttt tcccggggat cgcagtggtg agtaaccatg
     7441 catcatcagg agtacggata aaatgcttga tggtcggaag aggcataaat tccgtcagcc
     7501 agtttagtct gaccatctca tctgtaacat cattggcaac gctacctttg ccatgtttca
     7561 gaaacaactc tggcgcatcg ggcttcccat acaatcgata gattgtcgca cctgattgcc
     7621 cgacattatc gcgagcccat ttatacccat ataaatcagc atccatgttg gaatttaatc
     7681 gcggcctcga gcaagacgtt tcccgttgaa tatggctcat aacacccctt gtattactgt
     7741 ttatgtaagc agacagtttt attgttcatg atgatatatt tttatcttgt gcaatgtaac
     7801 atcagagatt ttgagacaca acgtggcttt cccccccccc cctgcaggtc cgacacgggg
     7861 atggatggcg ttcccgatca tggtcctgct tgcttcgggt ggcatcggaa tgccggcgct
     7921 gcaagcaatg ttgtccaggc aggtggatga ggaacgtcag gggcagctgc aaggctcact
     7981 ggcggcgctc accagcctga cctcgatcgt cggacccctc ctcttcacgg cgatctatgc
     8041 ggcttctata acaacgtgga acgggtgggc atggattgca ggcgctgccc tctacttgct
     8101 ctgcctgccg gcgctgcgtc gcgggctttg gagcggcgca gggcaacgag ccgatcgctg
     8161 atcgtggaaa cgataggcct atgccatgcg ggtcaaggcg acttccggca agctatacgc
     8221 gccctagaat tgtcaatttt aatcctctgt ttatcggcag ttcgtagagc gcgccgtgcg
     8281 tcccgagcga tactgagcga agcaagtgcg tcgagcagtg cccgcttgtt cctgaaatgc
     8341 cagtaaagcg ctggctgctg aacccccagc cggaactgac cccacaaggc cctagcgttt
     8401 gcaatgcacc aggtcatcat tgacccaggc gtgttccacc aggccgctgc ctcgcaactc
     8461 ttcgcaggct tcgccgacct gctcgcgcca cttcttcacg cgggtggaat ccgatccgca
     8521 catgaggcgg aaggtttcca gcttgagcgg gtacggctcc cggtgcgagc tgaaatagtc
     8581 gaacatccgt cgggccgtcg gcgacagctt gcggtacttc tcccatatga atttcgtgta
     8641 gtggtcgcca gcaaacagca cgacgatttc ctcgtcgatc aggacctggc aacgggacgt
     8701 tttcttgcca cggtccagga cgcggaagcg gtgcagcagc gacaccgatt ccaggtgccc
     8761 aacgcggtcg gacgtgaagc ccatcgccgt cgcctgtagg cgcgacaggc attcctcggc
     8821 cttcgtgtaa taccggccat tgatcgacca gcccaggtcc tggcaaagct cgtagaacgt
     8881 gaaggtgatc ggctcgccga taggggtgcg cttcgcgtac tccaacacct gctgccacac
     8941 cagttcgtca tcgtcggccc gcagctcgac gccggtgtag gtgatcttca cgtccttgtt
     9001 gacgtggaaa atgaccttgt tttgcagcgc ctcgcgcggg attttcttgt tgcgcgtggt
     9061 gaacagggca gagcgggccg tgtcgtttgg catcgctcgc atcgtgtccg gccacggcgc
     9121 aatatcgaac aaggaaagct gcatttcctt gatctgctgc ttcgtgtgtt tcagcaacgc
     9181 ggcctgcttg gcctcgctga cctgttttgc caggtcctcg ccggcggttt ttcgcttctt
     9241 ggtcgtcata gttcctcgcg tgtcgatggt catcgacttc gccaaacctg ccgcctcctg
     9301 ttcgagacga cgcgaacgct ccacggcggc cgatggcgcg ggcagggcag ggggagccag
     9361 ttgcacgctg tcgcgctcga tcttggccgt agcttgctgg accatcgagc cgacggactg
     9421 gaaggtttcg cggggcgcac gcatgacggt gcggcttgcg atggtttcgg catcctcggc
     9481 ggaaaacccc gcgtcgatca gttcttgcct gtatgccttc cggtcaaacg tccgattcat
     9541 tcaccctcct tgcgggattg ccccgactca cgccggggca atgtgccctt attcctgatt
     9601 tgacccgcct ggtgccttgg tgtccagata atccacctta tcggcaatga agtcggtccc
     9661 gtagaccgtc tggccgtcct tctcgtactt ggtattccga atcttgccct gcacgaatac
     9721 cagctccgcg aagtcgctct tcttgatgga gcgcatgggg acgtgcttgg caatcacgcg
     9781 caccccccgg ccgttttagc ggctaaaaaa gtcatggctc tgccctcggg cggaccacgc
     9841 ccatcatgac cttgccaagc tcgtcctgct tctcttcgat cttcgccagc agggcgagga
     9901 tcgtggcatc accgaaccgc gccgtgcgcg ggtcgtcggt gagccagagt ttcagcaggc
     9961 cgcccaggcg gcccaggtcg ccattgatgc gggccagctc gcggacgtgc tcatagtcca
    10021 cgacgcccgt gattttgtag ccctggccga cggccagcag gtaggcctac aggctcatgc
    10081 cggccgccgc cgccttttcc tcaatcgctc ttcgttcgtc tggaaggcag tacaccttga
    10141 taggtgggct gcccttcctg gttggcttgg tttcatcagc catccgcttg ccctcatctg
    10201 ttacgccggc ggtagccggc cagcctcgca gagcaggatt cccgttgagc accgccaggt
    10261 gcgaataagg gacagtgaag aaggaacacc cgctcgcggg tgggcctact tcacctatcc
    10321 tgcccggctg acgccgttgg atacaccaag gaaagtctac acgaaccctt tggcaaaatc
    10381 ctgtatatcg tgcgaaaaag gatggatata ccgaaaaaat cgctataatg accccgaagc
    10441 agggttatgc agcggaaaag atccgtc
//
