LOCUS       62056_12195        5102 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5102)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5102
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_signal     190..340
                     /label=Ad5 Psi
                     /note="packaging signal for adenovirus serotype 5"
     promoter        484..724
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA"
     enhancer        853..1156
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1157..1360
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             1482..2198
                     /codon_start=1
                     /label=EGFP
                     /note="enhanced GFP"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     polyA_signal    2225..2359
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(2379..2412)
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(2434..2450)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2458..2474)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2482..2512)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2527..2548)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(2836..3424)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3598..4455)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4456..4560)
                     /label=AmpR promoter
     repeat_region   5102
                     /label=ITR
                     /note="inverted terminal repeat of human adenovirus
                     serotype 5"
ORIGIN
        1 ttcatcaata atatacctta ttttggattg aagccaatat gataatgagg gggtggagtt
       61 tgtgacgtgg cgcggggcgt gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg
      121 atgttgcaag tgtggcggaa cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg
      181 tgtgcgccgg tgtacacagg aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt
      241 aaatttgggc gtaaccgagt aagatttggc cattttcgcg ggaaaactga ataagaggaa
      301 gtgaaatctg aataattttg tgttactcat agcgcgtaat atttgtctag ggccgcgggg
      361 actttgaccg tttacgtgga gactcgccca ggtgtttttc tcaggtgttt tccgcgttcc
      421 gggtcaaagt tggcgtttta ttattatagt cagntctaga ctcgagcggc cgcccccttc
      481 accgagggcc tatttcccat gattccttca tatttgcata tacgatacaa ggctgttaga
      541 gagataattg gaattaattt gactgtaaac acaaagatat tagtacaaaa tacgtgacgt
      601 agaaagtaat aatttcttgg gtagtttgca gttttaaaat tatgttttaa aatggactat
      661 catatgctta ccgtaacttg aaagtatttc gatttcttgg ctttatatat cttgtggaaa
      721 ggacgaaaca ccggtccgca gaattcggat ccattaggcg gccgcgtgga taaccgtatt
      781 accgccatgc attagttatt aatagtaatc aattacgggg tcattagttc atagcccata
      841 tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga
      901 cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt
      961 ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt
     1021 gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca
     1081 ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt
     1141 catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt
     1201 tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca
     1261 ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg
     1321 cggtaggcgt gtacggtggg aggtctatat aagcagagct ggtttagtga accgtcagat
     1381 ccgctagcac gcgtccaggt accatggata tcgccactaa cttctccctg ttgaaacaag
     1441 caggggatgt cgaagagaat cccgggccac cggtcgccac catggtgagc aagggcgagg
     1501 agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca
     1561 agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt
     1621 tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct
     1681 acggcgtgca gtgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt
     1741 ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact
     1801 acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga
     1861 agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca
     1921 acagccacaa cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca
     1981 agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca
     2041 cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtccg
     2101 ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg
     2161 ccgccgggat cactctcggc atggacgagc tgtacaagta agcttgagct cgtcgacttc
     2221 gagcaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     2281 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     2341 atcttatcat gtctggatcg tctagcatcg aagatccaat aacttcgtat agcatacatt
     2401 atacgaagtt ataagtagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg
     2461 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
     2521 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
     2581 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     2641 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     2701 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     2761 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     2821 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     2881 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     2941 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     3001 tctcccttcg ggaagcgtgg cgctttctca atgctcacgc tgtaggtatc tcagttcggt
     3061 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     3121 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     3181 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     3241 cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct
     3301 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     3361 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     3421 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     3481 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     3541 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     3601 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     3661 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     3721 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     3781 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     3841 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     3901 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     3961 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     4021 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     4081 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     4141 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     4201 cccggcgtca acacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     4261 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     4321 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     4381 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     4441 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
     4501 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
     4561 cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac
     4621 ctataaaaat aggcgtatca ctctaggcaa aatagcaccc tcccgctcca gaacaacata
     4681 cagcgcttca cagcggcagc ctaacagtca gccttaccag taaaaaagaa aacctattaa
     4741 aaaaacacca ctcgacacgg caccagctca atcagtcaca gtgtaaaaaa gggccaagtg
     4801 cagagcgagt atatatagga ctaaaaaatg acgtaacggt taaagtccac aaaaaacacc
     4861 cagaaaaccg cacgcgaacc tacgcccaga aacgaaagcc aaaaaaccca caacttcctc
     4921 aaatcgtcac ttccgttttc ccacgttacg taacttccca ttttaagaaa actacaattc
     4981 ccaacacata caagttactc cgccctaaaa cctacgtcac ccgccccgtt cccacgcccc
     5041 gcgccacgtc acaaactcca ccccctcatt atcatattgg cttcaatcca aaataaggta
     5101 ta
//
