LOCUS       V005613                 4911 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V005613
VERSION     V005613
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4911)
  AUTHORS   Gao H, Xu X.
  TITLE     pHB518
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4911)
  AUTHORS   Gao H, Xu X.
  TITLE     unpublished
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 4911)
  AUTHORS   Gao H, Xu X.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JUN-2007) Institute of Hydrobiology, Chinese Academy
            of Science, #7 Donghu South Rd, Wuchang, Wuhan, Hubei 430072, China
REFERENCE   4  (bases 1 to 4911)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 4911)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 3; type: "Journal Article"; journalName: "Submitted
            (20-JUN-2007) Institute of Hydrobiology, Chinese Academy of Science,
            #7 Donghu South Rd, Wuchang, Wuhan, Hubei 430072, China"
            SGRef: number: 4; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4911
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    106..127
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        142..172
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    180..196
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     204..220
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             445..1176
                     /gene="ermC"
                     /label="rRNA adenine N-6-methyltransferase"
                     /note="rRNA adenine N-6-methyltransferase from
                     Staphylococcus aureus. Accession#: P02979"
     CDS             complement(1317..1973)
                     /label="CmR"
                     /note="chloramphenicol acetyltransferase"
     promoter        complement(1974..2076)
                     /label="cat promoter"
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     CDS             complement(2267..3079)
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
     primer_bind     complement(3226..3242)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        3716..3820
                     /label="AmpR promoter"
     rep_origin      4141..4729
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
ORIGIN
        1 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
       61 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
      121 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
      181 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagcttgc
      241 atgcctgcag gcggccgcgg atccccgggt accgagctcg aattcgcgtg ctataattat
      301 actaatttta taaggaggaa aaaatatggg catttttagt atttttgtaa tcagcacagt
      361 tcattatcaa ccaaacaaaa aataagtggt tataatgaat cgttaataag caaaattcat
      421 ataaccaaat taaagagggt tataatgaac gagaaaaata taaaacacag tcaaaacttt
      481 attacttcaa aacataatat agataaaata atgacaaata taagattaaa tgaacatgat
      541 aatatctttg aaatcggctc aggaaaaggc cattttaccc ttgaattagt aaagaggtgt
      601 aatttcgtaa ctgccattga aatagaccat aaattatgca aaactacaga aaataaactt
      661 gttgatcacg ataatttcca agttttaaac aaggatatat tgcagtttaa atttcctaaa
      721 aaccaatcct ataaaatata tggtaatata ccttataaca taagtacgga tataatacgc
      781 aaaattgttt ttgatagtat agctaatgag atttatttaa tcgtggaata cgggtttgct
      841 aaaagattat taaatacaaa acgctcattg gcattacttt taatggcaga agttgatatt
      901 tctatattaa gtatggttcc aagagaatat tttcatccta aacctaaagt gaatagctca
      961 cttatcagat taagtagaaa aaaatcaaga atatcacaca aagataaaca aaagtataat
     1021 tatttcgtta tgaaatgggt taacaaagaa tacaagaaaa tatttacaaa aaatcaattt
     1081 aacaattcct taaaacatgc aggaattgac gatttaaaca atattagctt tgaacaattc
     1141 ttatctcttt tcaatagcta taaattattt aataagtaag ttaagggatg caggtcgact
     1201 ctagggggat ccgacggtaa gtctagtcga ctttctgcca ttcatccgct tattatcact
     1261 tattcaggcg tagcaccagg cgtttaaggg caccaataac tgccttaaaa aaattacgcc
     1321 ccgccctgcc actcatcgca gtactgttgt aattcattaa gcattctgcc gacatggaag
     1381 ccatcacaaa cggcatgatg aacctgaatc gccagcggca tcagcacctt gtcgccttgc
     1441 gtataatatt tgcccatggt gaaaacgggg gcgaagaagt tgtccatatt ggccacgttt
     1501 aaatcaaaac tggtgaaact cacccaggga ttggctgaga cgaaaaacat attctcaata
     1561 aaccctttag ggaaataggc caggttttca ccgtaacacg ccacatcttg cgaatatatg
     1621 tgtagaaact gccggaaatc gtcgtggtat tcactccaga gcgatgaaaa cgtttcagtt
     1681 tgctcatgga aaacggtgta acaagggtga acactatccc atatcaccag ctcaccgtct
     1741 ttcattgcca tacggaattc cggatgagca ttcatcaggc gggcaagaat gtgaataaag
     1801 gccggataaa acttgtgctt atttttcttt acggtcttta aaaaggccgt aatatccagc
     1861 tgaacggtct ggttataggt acattgagca actgactgaa atgcctcaaa atgttcttta
     1921 cgatgccatt gggatatatc aacggtggta tatccagtga tttttttctc cattttagct
     1981 tccttagctc ctgaaaatct cgataactca aaaaatacgc ccggtagtga tcttatttca
     2041 ttatggtgaa agttggaacc tcttacgtgc cgatcaacgt ctcattttcg ccaaaagttg
     2101 gcccagggct tcccggtatc aacagggaca ccaggattta tttattctgc gaagtgatct
     2161 tccgtcacag gtatttgtcg acttgacata cagtcggtac ccaagggtcc cgtcaagtca
     2221 gcgtaatgct ctgccagtgt tacaaccaat taaccaattc tgattagaaa aactcatcga
     2281 gcatcaaatg aaactgcaat ttattcatat caggattatc aataccatat ttttgaaaaa
     2341 gccgtttctg taatgaagga gaaaactcac cgaggcagtt ccataggatg gcaagatcct
     2401 ggtatcggtc tgcgattccg actcgtccaa catcaataca acctattaat ttcccctcgt
     2461 caaaaataag gttatcaagt gagaaatcac catgagtgac gactgaatcc ggtgagaatg
     2521 gcaaaagctt atgcatttct ttccagactt gttcaacagg ccagccatta cgctcgtcat
     2581 caaaatcact cgcatcaacc aaaccgttat tcattcgtga ttgcgcctga gcgagacgaa
     2641 atacgcgatc gctgttaaaa ggacaattac aaacaggaat cgaatgcaac cggcgcagga
     2701 acactgccag cgcatcaaca atattttcac ctgaatcagg atattcttct aatacctgga
     2761 atgctgtttt cccggggatc gcagtggtga gtaaccatgc atcatcagga gtacggataa
     2821 aatgcttgat ggtcggaaga ggcataaatt ccgtcagcca gtttagtctg accatctcat
     2881 ctgtaacatc attggcaacg ctacctttgc catgtttcag aaacaactct ggcgcatcgg
     2941 gcttcccata caatcgatag attgtcgcac ctgattgccc gacattatcg cgagcccatt
     3001 tatacccata taaatcagca tccatgttgg aatttaatcg cggcctcgag caagacgttt
     3061 cccgttgaat atggctcata acaccccttg tattactgtt tatgtaagca gacagtttta
     3121 ttgttcatga tgatatattt ttatcttgtg caatgtaaca tcagagattt tgagacacaa
     3181 cgtggctttc gggacctctg ggtacctgca ggcggccgcg aattcactgg ccgtcgtttt
     3241 acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc
     3301 ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt
     3361 gcgcagcctg aatggcgaat ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg
     3421 tatttcacac cgcatatggt gcactctcag tacaatctgc tctgatgccg catagttaag
     3481 ccagccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc
     3541 atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc
     3601 gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa
     3661 tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg
     3721 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     3781 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
     3841 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
     3901 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
     3961 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
     4021 gagcactttt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     4081 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     4141 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     4201 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     4261 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     4321 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     4381 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     4441 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     4501 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     4561 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     4621 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     4681 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     4741 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
     4801 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
     4861 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag a
//
