LOCUS       Exported                6603 bp DNA     circular SYN 01-NOV-2024
DEFINITION  Exported.
ACCESSION   V012859
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6603)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 6603)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6603
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1545..1563
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    1576..1683
                     /label=MCS
                     /note="pBluescript multiple cloning site"
     promoter        complement(1692..1710)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1720..1736)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      2082..2168
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      2260..2287
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     CDS             complement(2729..3376)
                     /gene="cat"
                     /label=Chloramphenicol acetyltransferase
                     /note="Chloramphenicol acetyltransferase from
                     Staphylococcus aureus. Accession#: P00485"
     CDS             complement(5338..6069)
                     /gene="ermC"
                     /label=rRNA adenine N-6-methyltransferase
                     /note="rRNA adenine N-6-methyltransferase from
                     Staphylococcus aureus. Accession#: P02979"
     rep_origin      6338..6603
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
       61 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
      121 gtgagcattg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
      181 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
      241 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
      301 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
      361 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
      421 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
      481 agtcagtgag cgaggaagcg gaagaagctt actttattgt tttgccattt taaaaggttc
      541 aaataaaata tttttcataa aattatcaga tgttttttaa aaaatgaaat aaaagaagaa
      601 aaaaatagaa aatacactaa cccaaagaag cgcgtaatat cgagggttta agagacaata
      661 agaaaaaaag attgaaaaat gacattaaat tcttgacagg gagagatagg tttgatagaa
      721 tataatagtt gtcgcgagag acgacgttga agcgcgacaa gctagaccat tgaaaactga
      781 ataaagaaga atgactcatg tgatgtagca atacatcaaa aatctgtcaa tcaataatga
      841 aagacaagcc atcaattcgg tgacttaaat actttatttg agagtttgat cctctagagt
      901 cgacctgcag gctttaacgt aggcaaagct cagggtagac tttgaatgga cagaaacatg
      961 acatatctct tgaaaggatg attgtggtgg tgaaaacaga taaaatctcc tcctgaatac
     1021 agtaaatcac attcaggagg agatacagga ggagataaaa ttgtttaaac aaatagacga
     1081 aaattatctg cgaaaagagc actttcacca ttatatgacg ttaacccgat gctcatatag
     1141 cttggtgatc aatctagaca tcacgaaatt gcatgcaata ttaaaagaaa aaaagctgaa
     1201 agtatatcct gtgcaaattt atttgttagc aagagctgtg caaaaaattc ctgagtttcg
     1261 gatggatcaa gtgaacgatg aacttggtta ctgggagatt ctccatccta gttatacgat
     1321 tctaaataaa gaaacaaaga cgttttcaag tatttggacg ccttttgatg aaaactttgc
     1381 tcagttttat aaaagctgtg tagccgatat tgaaacattt agcaaaagca gcaacctatt
     1441 tccgaaacct catatgccag aaaacatgtt caatatttca agtctaccgt ggattgattt
     1501 tacttctttt aaccttaatg tatctacaga tgaagctagc gcgcaattaa ccctcactaa
     1561 agggaacaaa agctggagct ccaccgcggt ggcggccgct ctagaactag tggatccccc
     1621 gggctgcagg aattcgatat caagcttatc gataccgtcg acctcgaggg ggggcccggt
     1681 acccaattcg ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg
     1741 tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt
     1801 cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag
     1861 cctgaattag agatcaattc cctgttttgg cggatgagag aagattttca gcctgataca
     1921 gattaaatca gaacgcagaa gcggtctgat aaaacagaat ttgcctggcg gcagtagcgc
     1981 ggtggtccca cctgacccca tgccgaactc agaagtgaaa cgccgtagcg ccgatggtag
     2041 tgtggggtct ccccatgcga gagtagggaa ctgccaggca tcaaataaaa cgaaaggctc
     2101 agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctcctgagta
     2161 ggacaaatcc gccgggagcg gatttgaacg ttgcgaagca acggcccgga gggtggcggg
     2221 caggacgccc gccataaact gccaggcatc aaattaagca gaaggccatc ctgacggatg
     2281 gcctttttgc gtttctacaa actcttcctg tcgtcatatc tacaagccat ccccccacag
     2341 atacggtaaa ctagcctcgt ttttgcatca ggaaagcagg gaattgatct gatgggtaga
     2401 accttgggct ttgcccaaac ccaccaacgg atggccgttg gattccaaca ttcaatcccc
     2461 ccaacccccc gcatctccgg ggggctccct cgccggttgg cgtcaaacaa acgagtttgt
     2521 ttgagtgctg aaagggttcg ggtagcactg agcgagtcaa ctccttaatc ctccctacgg
     2581 tccggttacc gttgactcac gccataccca tcgaatggcg aatggcggat ctggagctgt
     2641 aatataaaaa ccttcttcaa ctaacggggc aggttagtga cattagaaaa ccgactgtaa
     2701 aaagtacagt cggcattatc tcatattata aaagccagtc attaggccta tctgacaatt
     2761 cctgaataga gttcataaac aatcctgcat gataaccatc acaaacagaa tgatgtacct
     2821 gtaaagatag cggtaaatat attgaattac ctttattaat gaattttcct gctgtaataa
     2881 tgggtagaag gtaattacta ttattattga tatttaagtt aaacccagta aatgaagtcc
     2941 atggaataat agaaagagaa aaagcatttt caggtatagg tgttttggga aacaatttcc
     3001 ccgaaccatt atatttctct acatcagaaa ggtataaatc ataaaactct ttgaagtcat
     3061 tctttacagg agtccaaata ccagagaatg ttttagatac accatcaaaa attgtataaa
     3121 gtggctctaa cttatcccaa taacctaact ctccgtcgct attgtaacca gttctaaaag
     3181 ctgtatttga gtttatcacc cttgtcacta agaaaataaa tgcagggtaa aatttatatc
     3241 cttcttgttt tatgtttcgg tataaaacac taatatcaat ttctgtggtt atactaaaag
     3301 tcgtttgttg gttcaaataa tgattaaata tctcttttct cttccaattg tctaaatcaa
     3361 ttttattaaa gttcatttga tatgcctcct aaatttttat ctaaagtgaa tttaggaggc
     3421 ttacttgtct gctttcttca ttagaatcaa tcctttttta aaagtcaata ttactgtaac
     3481 ataaatatat attttaaaaa tatcccactt tatccaattt tcgtttgttg aactaatggg
     3541 tgctttagtt gaagaataaa gaccacatta aaaaatgtgg tcttttgtgt ttttttaaag
     3601 gatttgagcg tagcgaaaaa tccttttctt tcttatcttg ataataaggg taactattgc
     3661 cgcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcataagcc
     3721 ggtctttttc gattttggcg aagccgaaat cgggtctttt cttatcttga tactatatag
     3781 aaacaacatc atttttcaaa attaggtcaa agccttgtgt atcaagggtt tgatggttct
     3841 ttgacaggta aaaactcctt ctgctattat taaggtgtcg aatcaaaata atagaatgct
     3901 agagaactag ctcagaagga gtttttttgt tgatttattc atctgaaaat gattatagca
     3961 tcctcgaaga taaaaccgca acaggtaaaa agcgggattg gaaggggaaa aagagacgga
     4021 cgaacctcat ggcggagcat tacgaagcgt tagagagtaa gattggggca ccttactatg
     4081 gcaaaaaggc tgaaaaacta attagttgtg cagagtatct ttcgtttaag agagacccgg
     4141 agacgggcaa gttaaaactg tatcaagccc atttttgtaa agtgaggtta tgtccgatgt
     4201 gtgcgtggcg caggtcgtta aaaattgctt atcacaataa gttgatcgta gaggaagcca
     4261 atagacagta cggctgcgga tggatttttc tcacgctgac gattcgaaat gtaaagggag
     4321 aacggctgaa gccacaaatt tctgcgatga tggaaggctt taggaaactg ttccagtaca
     4381 aaaaagtaaa aacttcggtt cttggatttt tcagagcttt agagattacc aaaaatcatg
     4441 aagaagatac atatcatcct cattttcatg tgttgatacc agtaaggaaa aattattttg
     4501 ggaaaaacta tattaagcag gcggagtgga cgagcctttg gaaaaaggcg atgaaattgg
     4561 attacactcc aattgtcgat attcgtcgag tgaaaggtaa agctaagatt gacgctgaac
     4621 agattgaaaa cgatgtgcgg aacgcaatga tggagcaaaa agctgttctc gaaatctcta
     4681 aatatccggt taaggatacg gatgttgtgc gcggtaataa ggtgactgaa gacaatctga
     4741 acacggtgct ttacttggat gatgcgttgg cagctcgaag gttaattgga tacggtggca
     4801 ttttgaagga gatacataaa gagctgaatc ttggtgatgc ggaggacggc gatctggtca
     4861 agattgagga agaagatgac gaggttgcaa atggtgcatt tgaggttatg gcttattggc
     4921 atcctggcat taaaaattac ataatcaaat aaaaaaagca gacctttaga aggcctgctt
     4981 ttttaactaa cccatttgta ttgtgttgaa atatgttttg tatggtgcac tctcagtaca
     5041 atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg
     5101 ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg
     5161 agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc
     5221 gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gcgattcaca
     5281 aaaaataggc acacgaaaaa caagttaagg gatgcagttt atgcatccct taacttactt
     5341 attaaataat ttatagctat tgaaaagaga taagaattgt tcaaagctaa tattgtttaa
     5401 atcgtcaatt cctgcatgtt ttaaggaatt gttaaattga ttttttgtaa atattttctt
     5461 gtattctttg ttaacccatt tcataacgaa ataattatac ttttgtttat ctttgtgtga
     5521 tattcttgat ttttttctac ttaatctgat aagtgagcta ttcactttag gtttaggatg
     5581 aaaatattct cttggaacca tacttaatat agaaatatca acttctgcca ttaaaagtaa
     5641 tgccaatgag cgttttgtat ttaataatct tttagcaaac ccgtattcca cgattaaata
     5701 aatctcatta gctatactat caaaaacaat tttgcgtatt atatccgtac ttatgttata
     5761 aggtatatta ccatatattt tataggattg gtttttagga aatttaaact gcaatatatc
     5821 cttgtttaaa acttggaaat tatcgtgatc aacaagttta ttttctgtag ttttgcataa
     5881 tttatggtct atttcaatgg cagttacgaa attacacctc tttactaatt caagggtaaa
     5941 atggcctttt cctgagccga tttcaaagat attatcatgt tcatttaatc ttatatttgt
     6001 cattatttta tctatattat gttttgaagt aataaagttt tgactgtgtt ttatattttt
     6061 ctcgttcatt ataaccctct ttaatttggt tatatgaatt ttgcttatta acgattcatt
     6121 ataaccactt attttttgtt tggttgataa tgaactgtgc tgattacaaa aatactaaaa
     6181 atgcccatat tttttcctcc ttataaaatt agtataatta tagcacgaaa aggatctagg
     6241 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     6301 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     6361 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     6421 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     6481 ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     6541 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     6601 tta
//
