LOCUS       40924_24124        3661 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pHB168-66, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3661)
  AUTHORS   Xu X.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JUN-2007) Institute of Hydrobiology, Chinese Academy 
            of Science, #7 Donghu South Rd, Wuchang, Wuhan, Hubei 430072, China
REFERENCE   2  (bases 1 to 3661)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 3661)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (19-JUN-2007) Institute of Hydrobiology, Chinese Academy of Science,
            #7 Donghu South Rd, Wuchang, Wuhan, Hubei 430072, China"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3661
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             535..1347
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1440..1456)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(1464..1480)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1488..1518)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1533..1554)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(1842..2430)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2604..3461)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3462..3566)
                     /label=AmpR promoter
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaagggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acccagaggt
      421 cccgaaagcc acgttgtgtc tcaaaatctc tgatgttaca ttgcacaaga taaaaatata
      481 tcatcatgaa caataaaact gtctgcttac ataaacagta atacaagggg tgttatgagc
      541 catattcaac gggaaacgtc ttgctcgagg ccgcgattaa attccaacat ggatgctgat
      601 ttatatgggt ataaatgggc tcgcgataat gtcgggcaat caggtgcgac aatctatcga
      661 ttgtatggga agcccgatgc gccagagttg tttctgaaac atggcaaagg tagcgttgcc
      721 aatgatgtta cagatgagat ggtcagacta aactggctga cggaatttat gcctcttccg
      781 accatcaagc attttatccg tactcctgat gatgcatggt tactcaccac tgcgatcccc
      841 gggaaaacag cattccaggt attagaagaa tatcctgatt caggtgaaaa tattgttgat
      901 gcgctggcag tgttcctgcg ccggttgcat tcgattcctg tttgtaattg tccttttaac
      961 agcgatcgcg tatttcgtct cgctcaggcg caatcacgaa tgaataacgg tttggttgat
     1021 gcgagtgatt ttgatgacga gcgtaatggc tggcctgttg aacaagtctg gaaagaaatg
     1081 cataagcttt tgccattctc accggattca gtcgtcactc atggtgattt ctcacttgat
     1141 aaccttattt ttgacgaggg gaaattaata ggttgtattg atgttggacg agtcggaatc
     1201 gcagaccgat accaggatct tgccatccta tggaactgcc tcggtgagtt ttctccttca
     1261 ttacagaaac ggctttttca aaaatatggt attgataatc ctgatatgaa taaattgcag
     1321 tttcatttga tgctcgatga gtttttctaa tcagaattgg ttaattggtt gtaacactgg
     1381 cagagcatta cgctgacttg acgggaccct tgggtaccga gctcgaattc gtaatcatgg
     1441 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
     1501 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
     1561 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgga ttaatgaatc
     1621 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
     1681 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     1741 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     1801 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     1861 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     1921 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     1981 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcaatgc
     2041 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     2101 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     2161 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     2221 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     2281 aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     2341 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     2401 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     2461 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     2521 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     2581 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     2641 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     2701 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     2761 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     2821 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     2881 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     2941 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     3001 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     3061 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     3121 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     3181 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     3241 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     3301 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     3361 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     3421 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     3481 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     3541 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa
     3601 gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt
     3661 c
//
