LOCUS       V016805                10186 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V016805
VERSION     V016805
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 10186)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..10186
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             173..1129
                     /label="mSox2"
                     /note="Mus musculus transcription factor SOX-2 gene.
                     Belongs to the SRY-related HMG-box (SOX) family of
                     transcription factors, which is involved in the regulation
                     of embryonic development and in the determination of cell
                     fate"
     CDS             1205..2653
                     /label="mKlf4"
                     /note="Mouse Kruppel-like factor (Klf4) gene. Belongs to
                     the relatively large family of SP1-like transcription
                     factors and is involved in the regulation of proliferation,
                     differentiation, apoptosis and somatic cell reprogramming."
     CDS             2738..4099
                     /label="mMyc"
                     /note="Mus musculus myc proto-oncogene. Belongs to
                     myelocytomatosis (Myc) family of transcription factors.
                     Plays a role in cell cycle procession, apotosis and
                     cellular transformation"
     misc_feature    4116..4704
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     LTR             4852..5032
                     /label="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        5165..5269
                     /label="AmpR promoter"
     CDS             5270..6127
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      6301..6889
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    7300..7321
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        7336..7366
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     LTR             7675..8308
                     /label="3' LTR"
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    8355..8480
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    8977..9210
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             9395..9439
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             9588..9629
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     misc_feature    9732..9849
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     protein_bind    9925..9943
                     /label="tet operator"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10009..10027
                     /label="tet operator"
                     /note="bacterial operator O2 for the tetR and tetA genes"
ORIGIN
        1 tgatagagaa aagtgaaagt cgagctcggt acccgggtcg aggtaggcat gtacggtggg
       61 aggcctatat aagcagagct cgtttagtga accgtcagat cgcctggaga cgccatccac
      121 gctgttttga cctccataga agacaccggg accgatccag cctgcggccg ccatgtataa
      181 catgatggag acggagctga agccgccggg cccgcagcaa gcttcggggg gcggcggcgg
      241 aggaggcaac gccacggcgg cggcaaccgg cggcaaccag aagaacagcc cggaccgcgt
      301 caagaggccc atgaacgcct tcatggtatg gtcccggggg cagcggcgta agatggccca
      361 ggagaacccc aagatgcaca actcggagat cagcaagcgc ctgggcgcgg agtggaaact
      421 tttgtccgag accgagaagc ggccgttcat cgacgaggcc aagcggctgc gcgctctgca
      481 catgaaggag cacccggatt ataaataccg gccgcggcgg aaaaccaaga cgctcatgaa
      541 gaaggataag tacacgcttc ccggaggctt gctggccccc ggcgggaaca gcatggcgag
      601 cggggttggg gtgggcgccg gcctgggtgc gggcgtgaac cagcgcatgg acagctacgc
      661 gcacatgaac ggctggagca acggcagcta cagcatgatg caggagcagc tgggctaccc
      721 gcagcacccg ggcctcaacg ctcacggcgc ggcacagatg caaccgatgc accgctacga
      781 cgtcagcgcc ctgcagtaca actccatgac cagctcgcag acctacatga acggctcgcc
      841 cacctacagc atgtcctact cgcagcaggg cacccccggt atggcgctgg gctccatggg
      901 ctctgtggtc aagtccgagg ccagctccag cccccccgtg gttacctctt cctcccactc
      961 cagggcgccc tgccaggccg gggacctccg ggacatgatc agcatgtacc tccccggcgc
     1021 cgaggtgccg gagcccgctg cgcccagtag actgcacatg gcccagcact accagagcgg
     1081 cccggtgccc ggcacggcca ttaacggcac actgcccctg tcgcacatgg catgcggctc
     1141 cggcgagggc aggggaagtc ttctaacatg cggggacgtg gaggaaaatc ccggcccact
     1201 cgagatgagg cagccacctg gcgagtctga catggctgtc agcgacgctc tgctcccgtc
     1261 cttctccacg ttcgcgtccg gcccggcggg aagggagaag acactgcgtc cagcaggtgc
     1321 cccgactaac cgttggcgtg aggaactctc tcacatgaag cgacttcccc cacttcccgg
     1381 ccgcccctac gacctggcgg cgacggtggc cacagacctg gagagtggcg gagctggtgc
     1441 agcttgcagc agtaacaacc cggccctcct agcccggagg gagaccgagg agttcaacga
     1501 cctcctggac ctagacttta tcctttccaa ctcgctaacc caccaggaat cggtggccgc
     1561 caccgtgacc acctcggcgt cagcttcatc ctcgtcttcc ccagcgagca gcggccctgc
     1621 cagcgcgccc tccacctgca gcttcagcta tccgatccgg gccgggggtg acccgggcgt
     1681 ggctgccagc aacacaggtg gagggctcct ctacagccga gaatctgcgc cacctcccac
     1741 ggcccccttc aacctggcgg acatcaatga cgtgagcccc tcgggcggct tcgtggctga
     1801 gctcctgcgg ccggagttgg acccagtata cattccgcca cagcagcctc agccgccagg
     1861 tggcgggctg atgggcaagt ttgtgctgaa ggcgtctctg accacccctg gcagcgagta
     1921 cagcagccct tcggtcatca gtgttagcaa aggaagccca gacggcagcc accccgtggt
     1981 agtggcgccc tacagcggtg gcccgccgcg catgtgcccc aagattaagc aagaggcggt
     2041 cccgtcctgc acggtcagcc ggtccctaga ggcccatttg agcgctggac cccagctcag
     2101 caacggccac cggcccaaca cacacgactt ccccctgggg cggcagctcc ccaccaggac
     2161 tacccctaca ctgagtcccg aggaactgct gaacagcagg gactgtcacc ctggcctgcc
     2221 tcttccccca ggattccatc cccatccggg gcccaactac cctcctttcc tgccagacca
     2281 gatgcagtca caagtcccct ctctccatta tcaagagctc atgccaccgg gttcctgcct
     2341 gccagaggag cccaagccaa agaggggaag aaggtcgtgg ccccggaaaa gaacagccac
     2401 ccacacttgt gactatgcag gctgtggcaa aacctatacc aagagttctc atctcaaggc
     2461 acacctgcga actcacacag gcgagaaacc ttaccactgt gactgggacg gctgtgggtg
     2521 gaaattcgcc cgctccgatg aactgaccag gcactaccgc aaacacacag ggcaccggcc
     2581 ctttcagtgc cagaagtgcg acagggcctt ttccaggtcg gaccaccttg ccttacacat
     2641 gaagaggcac tttctcgagg gctcgggcca gtgtactaat tatgctctct tgaaattggc
     2701 tggagatgtt gagagcaacc caggtcccgg cgcgccactg gatttccttt gggcgttgga
     2761 aaccccgcag acagccacga cgatgcccct caacgtgaac ttcaccaaca ggaactatga
     2821 cctcgactac gactccgtac agccctattt catctgcgac gaggaagaga atttctatca
     2881 ccagcaacag cagagcgagc tgcagccgcc cgcgcccagt gaggatatct ggaagaaatt
     2941 cgagctgctt cccaccccgc ccctgtcccc gagccgccgc tccgggctct gctctccatc
     3001 ctatgttgcg gtcgctacgt ccttctcccc aagggaagac gatgacggcg gcggtggcaa
     3061 cttctccacc gccgatcagc tggagatgat gaccgagtta cttggaggag acatggtgaa
     3121 ccagagcttc atctgcgatc ctgacgacga gaccttcatc aagaacatca tcatccagga
     3181 ctgtatgtgg agcggtttct cagccgctgc caagctggtc tcggagaagc tggcctccta
     3241 ccaggctgcg cgcaaagaca gcaccagcct gagccccgcc cgcgggcaca gcgtctgctc
     3301 cacctccagc ctgtacctgc aggacctcac cgccgccgcg tccgagtgca ttgacccctc
     3361 agtggtcttt ccctacccgc tcaacgacag cagctcgccc aaatcctgta cctcgtccga
     3421 ttccacggcc ttctctcctt cctcggactc gctgctgtcc tccgagtcct ccccacgggc
     3481 cagccctgag cccctagtgc tgcatgagga gacaccgccc accaccagca gcgactctga
     3541 agaagagcaa gaagatgagg aagaaattga tgtggtgtct gtggagaaga ggcaaacccc
     3601 tgccaagagg tcggagtcgg gctcatctcc atcccgaggc cacagcaaac ctccgcacag
     3661 cccactggtc ctcaagaggt gccacgtctc cactcaccag cacaactacg ccgcaccccc
     3721 ctccacaagg aaggactatc cagctgccaa gagggccaag ttggacagtg gcagggtcct
     3781 gaagcagatc agcaacaacc gcaagtgctc cagccccagg tcctcagaca cggaggaaaa
     3841 cgacaagagg cggacacaca acgtcttgga acgtcagagg aggaacgagc tgaagcgcag
     3901 cttttttgcc ctgcgtgacc agatccctga attggaaaac aacgaaaagg cccccaaggt
     3961 agtgatcctc aaaaaagcca ccgcctacat cctgtccatt caagcagacg agcacaagct
     4021 cacctctgaa aaggacttat tgaggaaacg acgagaacag ttgaaacaca aactcgaaca
     4081 gcttcgaaac tctggtgcat aaatcgatag atcctaatca acctctggat tacaaaattt
     4141 gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg
     4201 ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt
     4261 ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg
     4321 tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc
     4381 agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg
     4441 cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt
     4501 tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc tggattctgc
     4561 gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg
     4621 gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga
     4681 tctccctttg ggccgcctcc ccgcctggta cctttaagac caatgactta caaggcagct
     4741 gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa
     4801 cgaagacaag atcacctgca ggacaggcgc gccctgcttt ttgcttgtac tgggtctctc
     4861 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
     4921 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
     4981 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cacccgggcg
     5041 attaaggaaa gggctagatc attcttgaag acgaaagggc ctcgtgatac gcctattttt
     5101 ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa
     5161 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     5221 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
     5281 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
     5341 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
     5401 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
     5461 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc
     5521 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
     5581 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
     5641 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
     5701 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
     5761 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
     5821 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
     5881 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
     5941 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
     6001 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     6061 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     6121 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
     6181 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     6241 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     6301 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     6361 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     6421 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     6481 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     6541 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     6601 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     6661 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     6721 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     6781 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     6841 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     6901 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
     6961 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
     7021 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
     7081 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagcaagc tcatggctga
     7141 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
     7201 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctccccgt ggcacgacag
     7261 gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca
     7321 ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag
     7381 cggataacaa tttcacacag gaaacagcta tgacatgatt acgaatttca caaataaagc
     7441 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
     7501 ctggatcaac tggataactc aagctaacca aaatcatccc aaacttccca ccccataccc
     7561 tattaccact gccaattacc tgtggtttca tttactctaa acctgtgatt cctctgaatt
     7621 attttcattt taaagaaatt gtatttgtta aatatgtact acaaacttag tagttggaag
     7681 ggctaattca ctcccaaaga agacaagata tccttgatct gtggatctac cacacacaag
     7741 gctacttccc tgattagcag aactacacac cagggccagg ggtcagatat ccactgacct
     7801 ttggatggtg ctacaagcta gtaccagttg agccagataa ggtagaagag gccaataaag
     7861 gagagaacac cagcttgtta caccctgtga gcctgcatgg gatggatgac ccggagagag
     7921 aagtgttaga gtggaggttt gacagccgcc tagcatttca tcacgtggcc cgagagctgc
     7981 atccggagta cttcaagaac tgctgatatc gagcttgcta caagggactt tccgctgggg
     8041 actttccagg gaggcgtggc ctgggcggga ctggggagtg gcgagccctc agatcctgca
     8101 tataagcagc tgctttttgc ctgtactggg tctctctggt tagaccagat ctgagcctgg
     8161 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
     8221 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
     8281 ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag
     8341 ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag
     8401 gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc tagaaggaga
     8461 gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga tgggaaaaaa
     8521 ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta tgggcaagca
     8581 gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa ggctgtagac
     8641 aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt agatcattat
     8701 ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa gacaccaagg
     8761 aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg
     8821 ccggccgctg atcttcagac ctggaggagg agatatgagg gacaattgga gaagtgaatt
     8881 atataaatat aaagtagtaa aaattgaacc attaggagta gcacccacca aggcaaagag
     8941 aagagtggtg cagagagaaa aaagagcagt gggaatagga gctttgttcc ttgggttctt
     9001 gggagcagca ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac aggccagaca
     9061 attattgtct ggtatagtgc agcagcagaa caatttgctg agggctattg aggcgcaaca
     9121 gcatctgttg caactcacag tctggggcat caagcagctc caggcaagaa tcctggctgt
     9181 ggaaagatac ctaaaggatc aacagctcct ggggatttgg ggttgctctg gaaaactcat
     9241 ttgcaccact gctgtgcctt ggaatgctag ttggagtaat aaatctctgg aacagatttg
     9301 gaatcacacg acctggatgg agtgggacag agaaattaac aattacacaa gcttaataca
     9361 ctccttaatt gaagaatcgc aaaaccagca agaaaagaat gaacaagaat tattggaatt
     9421 agataaatgg gcaagtttgt ggaattggtt taacataaca aattggctgt ggtatataaa
     9481 attattcata atgatagtag gaggcttggt aggtttaaga atagtttttg ctgtactttc
     9541 tatagtgaat agagttaggc agggatattc accattatcg tttcagaccc acctcccaac
     9601 cccgagggga cccgacaggc ccgaaggaat agaagaagaa ggtggagaga gagacagaga
     9661 cagatccatt cgattagtga acggatctcg acggtatcgc cgaattcaca aatggcagta
     9721 ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata
     9781 gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaaatt
     9841 caaaattttc gggtttatta cagggacagc agagatccag tttggactag tccacaccct
     9901 aactgacaca ctcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagtt
     9961 taccactccc tatcagtgat agagaaaagt gaaagtcgag tttaccactc cctatcagtg
    10021 atagagaaaa gtgaaagtcg agtttaccac tccctatcag tgatagagaa aagtgaaagt
    10081 cgagtttacc actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta
    10141 tcagtgatag agaaaagtga aagtcgagtt taccactccc tatcag
//
