LOCUS       V017337                 8003 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V017337
VERSION     V017337
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8003)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..8003
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..399
                     /label="UbC promoter"
                     /note="human ubiquitin C promoter"
     CDS             1146..1172
                     /label="HA"
                     /note="HA (human influenza hemagglutinin) epitope tag"
     CDS             1263..1973
                     /label="superfolder GFP"
                     /note="GFP variant that folds robustly even when fused to
                     poorly folded proteins (Nager et al., 2011)"
     misc_feature    2176..2764
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     LTR             2839..3072
                     /label="3' LTR (Delta-U3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        3277..3381
                     /label="AmpR promoter"
     CDS             3382..4239
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      4413..5001
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    5412..5433
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        5448..5478
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    5486..5502
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5510..5526
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     LTR             5787..6420
                     /label="3' LTR"
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    6467..6592
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    7089..7322
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             7507..7551
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             7700..7741
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     misc_feature    7844..7961
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
ORIGIN
        1 ggcctccgcg ccgggttttg gcgcctcccg cgggcgcccc cctcctcacg gcgagcgctg
       61 ccacgtcaga cgaagggcgc agcgagcgtc ctgatccttc cgcccggacg ctcaggacag
      121 cggcccgctg ctcataagac tcggccttag aaccccagta tcagcagaag gacattttag
      181 gacgggactt gggtgactct agggcactgg ttttctttcc agagagcgga acaggcgagg
      241 aaaagtagtc ccttctcggc gattctgcgg agggatctcc gtggggcggt gaacgccgat
      301 gattatataa ggacgcgccg ggtgtggcac agctagttcc gtcgcagccg ggatttgggt
      361 cgcggttctt gtttgtggat cgctgtgatc gtcacttggc ggccatgggc ccagatattg
      421 ttatgaccca gtccccgtcc tccctgtccg caagcgttgg cgaccgtgtg accattacct
      481 gccgttcctc caccggcgct gtgaccacca gcaactacgc cagctgggtg caggagaagc
      541 cgggcaaact gttcaagggt ctgatcggcg gtaccaataa ccgtgccccg ggtgtgccaa
      601 gccgctttag cggcagcctg atcggtgaca aagctaccct gaccattagc agcctgcagc
      661 cggaggattt cgccacctac ttttgcgcgc tgtggtatag caaccactgg gtgttcggcc
      721 agggtaccaa agtggaactg aagcgtggcg gtgggggttc tggaggtggt gggagtggcg
      781 gtggcggttc tagcggaggg ggttccgagg tgaaactgct ggagagcggt ggcggtctgg
      841 tgcagccggg cggtagcctg aagctgagct gcgccgtgag cggcttcagc ctgaccgact
      901 acggtgtgaa ctgggtgcgt caggccccag gtcgcggtct ggaatggatc ggcgtgattt
      961 ggggcgacgg tattaccgat tataacagcg ccctgaagga ccgttttatc attagcaaag
     1021 ataacggcaa gaacaccgtg tacctgcaga tgagcaaggt gcgcagcgac gataccgcgc
     1081 tgtattattg cgttaccggc ctgttcgact attggggtca aggcaccctg gttaccgtta
     1141 gcagctaccc atacgatgtt ccagattacg ctggtggagg cggaggttct gggggaggag
     1201 gtagtggcgg tggtggttca ggaggcggcg gaagcttgga tccaggtgga ggtggaagcg
     1261 gtagcaaagg agaagaactt ttcactggag ttgtcccaat tcttgttgaa ttagatggtg
     1321 atgttaatgg gcacaaattt tctgtccgtg gagagggtga aggtgatgct acaaacggaa
     1381 aactcaccct taaatttatt tgcactactg gaaaactacc tgttccgtgg ccaacacttg
     1441 tcactactct gacctatggt gttcaatgct tttcccgtta tccggatcac atgaaacggc
     1501 atgacttttt caagagtgcc atgcccgaag gttatgtaca ggaacgcact atatctttca
     1561 aagatgacgg gacctacaag acgcgtgctg aagtcaagtt tgaaggtgat acccttgtta
     1621 atcgtatcga gttaaagggt attgatttta aagaagatgg aaacattctt ggacacaaac
     1681 tcgagtacaa ctttaactca cacaatgtat acatcacggc agacaaacaa aagaatggaa
     1741 tcaaagctaa cttcaaaatt cgccacaacg ttgaagatgg ttccgttcaa ctagcagacc
     1801 attatcaaca aaatactcca attggcgatg gccctgtcct tttaccagac aaccattacc
     1861 tgtcgacaca atctgtcctt tcgaaagatc ccaacgaaaa gcgtgaccac atggtccttc
     1921 ttgagtttgt aactgctgct gggattacac atggcatgga tgagctctac aaaggtggag
     1981 gtcggaccga agagtacaag cttatcctga acggtaaaac cctgaaaggt gaaaccacca
     2041 ccgaagctgt tgacgctgct accgcggaaa aagttttcaa acagtacgct aacgacaacg
     2101 gtgttgacgg tgaatggacc tacgacgacg ctaccaaaac cttcacggta accgcataat
     2161 gaatcgatag atcctaatca acctctggat tacaaaattt gtgaaagatt gactggtatt
     2221 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat
     2281 gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct
     2341 ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct
     2401 gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc
     2461 gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg
     2521 acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc
     2581 tttccttggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac
     2641 gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg
     2701 cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc
     2761 ccgcctgaga tcctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt
     2821 taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa gatctgcttt
     2881 ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac
     2941 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg
     3001 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga
     3061 aaatctctag cagtagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga
     3121 aatgaatatc agagagtgag aggcccgggt taattaagga aagggctaga tcattcttga
     3181 agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt
     3241 tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
     3301 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     3361 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
     3421 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
     3481 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
     3541 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
     3601 ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata
     3661 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
     3721 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
     3781 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
     3841 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
     3901 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact
     3961 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
     4021 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
     4081 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
     4141 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
     4201 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
     4261 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
     4321 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     4381 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     4441 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     4501 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
     4561 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     4621 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     4681 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     4741 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     4801 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     4861 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     4921 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     4981 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     5041 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
     5101 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
     5161 tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg
     5221 ccgattcatt aatgcagcaa gctcatggct gactaatttt ttttatttat gcagaggccg
     5281 aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
     5341 gcttttgcaa aaagctcccc gtggcacgac aggtttcccg actggaaagc gggcagtgag
     5401 cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg
     5461 cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc
     5521 tatgacatga ttacgaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     5581 ttgtccaaac tcatcaatgt atcttatcat gtctggatca actggataac tcaagctaac
     5641 caaaatcatc ccaaacttcc caccccatac cctattacca ctgccaatta cctgtggttt
     5701 catttactct aaacctgtga ttcctctgaa ttattttcat tttaaagaaa ttgtatttgt
     5761 taaatatgta ctacaaactt agtagttgga agggctaatt cactcccaaa gaagacaaga
     5821 tatccttgat ctgtggatct accacacaca aggctacttc cctgattagc agaactacac
     5881 accagggcca ggggtcagat atccactgac ctttggatgg tgctacaagc tagtaccagt
     5941 tgagccagat aaggtagaag aggccaataa aggagagaac accagcttgt tacaccctgt
     6001 gagcctgcat gggatggatg acccggagag agaagtgtta gagtggaggt ttgacagccg
     6061 cctagcattt catcacgtgg cccgagagct gcatccggag tacttcaaga actgctgata
     6121 tcgagcttgc tacaagggac tttccgctgg ggactttcca gggaggcgtg gcctgggcgg
     6181 gactggggag tggcgagccc tcagatcctg catataagca gctgcttttt gcctgtactg
     6241 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
     6301 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
     6361 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
     6421 gtggcgcccg aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag
     6481 gactcggctt gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc
     6541 aaaaattttg actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa
     6601 gcgggggaga attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa
     6661 atataaatta aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc
     6721 tggcctgtta gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct
     6781 tcagacagga tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt
     6841 gcatcaaagg atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca
     6901 aaacaaaagt aagaccaccg cacagcaagc ggccggccgc tgatcttcag acctggagga
     6961 ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa
     7021 ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca
     7081 gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca
     7141 gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag
     7201 aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc
     7261 atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc
     7321 ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct
     7381 agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac
     7441 agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag
     7501 caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg
     7561 tttaacataa caaattggct gtggtatata aaattattca taatgatagt aggaggcttg
     7621 gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat
     7681 tcaccattat cgtttcagac ccacctccca accccgaggg gacccgacag gcccgaagga
     7741 atagaagaag aaggtggaga gagagacaga gacagatcca ttcgattagt gaacggatct
     7801 cgacggtatc gccgaattca caaatggcag tattcatcca caattttaaa agaaaagggg
     7861 ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa
     7921 ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacagggaca
     7981 gcagagatcc agtttggact agt
//
