LOCUS       V016704                 9159 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V016704
VERSION     V016704
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9159)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..9159
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label="3' LTR"
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1303..1536
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             1721..1765
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             1914..1955
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     misc_feature    2058..2175
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     enhancer        2299..2602
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2603..2806
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     protein_bind    2808..2826
                     /label="tet operator"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     CDS             3343..4053
                     /label="mTagBFP2"
                     /note="enhanced monomeric blue fluorescent protein (Subach
                     et al., 2011)"
     CDS             complement(4819..5511)
                     /label="TagBFP2"
                     /note="Also known as mTagBFP2; improved variant of TagBFP
                     generated by I174A mutation"
     CDS             5518..5538
                     /label="SV40 NLS"
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     misc_feature    5549..6137
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     LTR             6212..6445
                     /label="3' LTR (Delta-U3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        6650..6754
                     /label="AmpR promoter"
     CDS             6755..7612
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      7786..8374
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    8785..8806
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        8821..8851
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    8859..8875
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8883..8899
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttgacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagcgat
     2221 gctagctgat gcgggcccta gttattaata gtaatcaatt acggggtcat tagttcatag
     2281 cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc
     2341 caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg
     2401 gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca
     2461 tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc
     2521 ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt
     2581 attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata
     2641 gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt
     2701 ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca
     2761 aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctctcc ctatcagtga
     2821 tagagatctc cctatcagtg atagagatcg tcgacgagct cgtttagtga accgtcagat
     2881 cgcctggaga cgccatccac gctgttttga cctccataga agacaccggg accgatccag
     2941 cctaccggtg gaagtgggcg cgccaccatg gcttctaact ttactcagtt cgttctcgtc
     3001 gacaatggcg gaactggcga cgtgactgtc gccccaagca acttcgctaa cgggatcgct
     3061 gaatggatca gctctaactc gcgttcacag gcttacaaag taacctgtag cgttcgtcag
     3121 agctctgcgc agaatcgcaa atacaccatc aaagtcgagg tgcctaaagg cgcctggcgt
     3181 tcgtacttaa atatggaact aaccattcca attttcgcca cgaattccga ctgcgagctt
     3241 attgttaagg caatgcaagg tctcctaaaa gatggaaacc cgattccctc agcaatcgca
     3301 gcaaactccg gcatctacac tagtggctct ggatccggaa gtatggtgtc taagggcgaa
     3361 gagctgatta aggagaacat gcacatgaag ctgtacatgg agggcaccgt ggacaaccat
     3421 cacttcaagt gcacatccga gggcgaaggc aagccctacg agggcaccca gaccatgaga
     3481 atcaaggtgg tcgagggcgg ccctctcccc ttcgccttcg acatcctggc tactagcttc
     3541 ctctacggca gcaagacctt catcaaccac acccagggca tccccgactt cttcaagcag
     3601 tccttccctg agggcttcac atgggagaga gtcaccacat acgaagacgg gggcgtgctg
     3661 accgctaccc aggacaccag cctccaggac ggctgcctca tctacaacgt caagatcaga
     3721 ggggtgaact tcacatccaa cggccctgtg atgcagaaga aaacactcgg ctgggaggcc
     3781 ttcaccgaga cgctgtaccc cgctgacggc ggcctggaag gcagaaacga catggccctg
     3841 aagctcgtgg gcgggagcca tctgatcgca aacgccaaga ccacatatag atccaagaaa
     3901 cccgctaaga acctcaagat gcctggcgtc tactatgtgg actacagact ggaaagaatc
     3961 aaggaggcca acaacgaaac ctacgtcgag cagcacgagg tggcagtggc cagatactgc
     4021 gacctcccta gcaaactggg gcacaagctt aattctggcg gccgctctgg catggtgtct
     4081 aagggcgaag agctgattaa ggagaacatg cacatgaagc tgtacatgga gggcaccgtg
     4141 gacaaccatc acttcaagtg cacatccgag ggcgaaggca agccctacga gggcacccag
     4201 accatgagaa tcaaggtggt cgagggcggc cctctcccct tcgccttcga catcctggct
     4261 actagcttcc tctacggcag caagaccttc atcaaccaca cccagggcat ccccgacttc
     4321 ttcaagcagt ccttccctga gggcttcaca tgggagagag tcaccacata cgaagacggg
     4381 ggcgtgctga ccgctaccca ggacaccagc ctccaggacg gctgcctcat ctacaacgtc
     4441 aagatcagag gggtgaactt cacatccaac ggccctgtga tgcagaagaa aacactcggc
     4501 tgggaggcct tcaccgagac gctgtacccc gctgacggcg gcctggaagg cagaaacgac
     4561 atggccctga agctcgtggg cgggagccat ctgatcgcaa acgccaagac cacatataga
     4621 tccaagaaac ccgctaagaa cctcaagatg cctggcgtct actatgtgga ctacagactg
     4681 gaaagaatca aggaggccaa caacgaaacc tacgtcgagc agcacgaggt ggcagtggcc
     4741 agatactgcg acctccctag caaactgggg cacaagctta atggtagcat cgatggtagc
     4801 atggtgtcta agggcgaaga gctgattaag gagaacatgc acatgaagct gtacatggag
     4861 ggcaccgtgg acaaccatca cttcaagtgc acatccgagg gcgaaggcaa gccctacgag
     4921 ggcacccaga ccatgagaat caaggtggtc gagggcggcc ctctcccctt cgccttcgac
     4981 atcctggcta ctagcttcct ctacggcagc aagaccttca tcaaccacac ccagggcatc
     5041 cccgacttct tcaagcagtc cttccctgag ggcttcacat gggagagagt caccacatac
     5101 gaagacgggg gcgtgctgac cgctacccag gacaccagcc tccaggacgg ctgcctcatc
     5161 tacaacgtca agatcagagg ggtgaacttc acatccaacg gccctgtgat gcagaagaaa
     5221 acactcggct gggaggcctt caccgagacg ctgtaccccg ctgacggcgg cctggaaggc
     5281 agaaacgaca tggccctgaa gctcgtgggc gggagccatc tgatcgcaaa cgccaagacc
     5341 acatatagat ccaagaaacc cgctaagaac ctcaagatgc ctggcgtcta ctatgtggac
     5401 tacagactgg aaagaatcaa ggaggccaac aacgaaacct acgtcgagca gcacgaggtg
     5461 gcagtggcca gatactgcga cctccctagc aaactggggc acaagcttaa tctcgagcca
     5521 aagaaaaagc ggaaagtgta atctagataa tcaacctctg gattacaaaa tttgtgaaag
     5581 attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat
     5641 gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc
     5701 ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg
     5761 cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct
     5821 ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct
     5881 tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg
     5941 gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac
     6001 gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct
     6061 gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct
     6121 ttgggccgcc tccccgcctg agatccttta agaccaatga cttacaaggc agctgtagat
     6181 cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga
     6241 caagatctgc tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag
     6301 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     6361 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     6421 tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc atcttattat tcagtattta
     6481 taacttgcaa agaaatgaat atcagagagt gagaggcccg ggttaattaa ggaaagggct
     6541 agatcattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat
     6601 gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     6661 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     6721 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
     6781 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
     6841 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
     6901 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
     6961 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact
     7021 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
     7081 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
     7141 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
     7201 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
     7261 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
     7321 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
     7381 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
     7441 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
     7501 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
     7561 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
     7621 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     7681 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     7741 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     7801 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     7861 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     7921 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     7981 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     8041 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     8101 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     8161 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     8221 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     8281 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     8341 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     8401 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     8461 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
     8521 cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct
     8581 ccccgcgcgt tggccgattc attaatgcag caagctcatg gctgactaat tttttttatt
     8641 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt
     8701 tttggaggcc taggcttttg caaaaagctc cccgtggcac gacaggtttc ccgactggaa
     8761 agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc
     8821 tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca
     8881 cacaggaaac agctatgaca tgattacgaa tttcacaaat aaagcatttt tttcactgca
     8941 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga tcaactggat
     9001 aactcaagct aaccaaaatc atcccaaact tcccacccca taccctatta ccactgccaa
     9061 ttacctgtgg tttcatttac tctaaacctg tgattcctct gaattatttt cattttaaag
     9121 aaattgtatt tgttaaatat gtactacaaa cttagtagt
//
