LOCUS       .                      13790 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     promoter        1..204
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     protein_bind    206..224
                     /label="tet operator"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     CDS             471..887
                     /label="gag (truncated)"
                     /note="truncated Moloney murine leukemia virus (MMLV) gag
                     gene lacking the start codon"
     CDS             2070..2090
                     /label="SV40 NLS"
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     CDS             2103..5345
                     /label="NmdCas9"
                     /note="catalytically dead mutant of the Cas9 endonuclease
                     from the Neisseria meningitidis Type II CRISPR/Cas system"
     CDS             5364..5384
                     /label="SV40 NLS"
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     CDS             5397..6104
                     /label="mCherry"
                     /note="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
     misc_feature    7578..8166
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     LTR             8241..8474
                     /label="3' LTR (ΔU3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        8679..8783
                     /label="AmpR promoter"
     CDS             8784..9641
                     /label="AmpR"
                     /note="β-lactamase"
     protein_bind    10814..10835
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        10850..10880
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    10888..10904
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
     primer_bind     10912..10928
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     LTR             11189..11822
                     /label="3' LTR"
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    11869..11994
                     /label="HIV-1 Ψ"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    12491..12724
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             12909..12953
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             13102..13143
                     /gene="Protein Tat"
                     /label="Protein Tat"
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
     misc_feature    13246..13363
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     enhancer        13487..13790
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
      181 tgggaggtct atataagcag agctctccct atcagtgata gagatctccc tatcagtgat
      241 agagatcgtc gacgagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc
      301 tgttttgacc tccatagaag acaccgggac cgatccagcc taccggttaa ggtgaacgcg
      361 tgcaggctgg cgccaccatg gccatgttca tacctcttat cttcctccca cagctcctgg
      421 gcaacgtgct ggtctgtgtg ctggcccatc actttggcaa agaattcatg ggccagactg
      481 ttaccactcc cttaagtttg accttaggtc actggaaaga tgtcgagcgg atcgctcaca
      541 accagtcggt agatgtcaag aagagacgtt gggttacctt ctgctctgca gaatggccaa
      601 cctttaacgt cggatggccg cgagacggca cctttaaccg agacctcatc acccaggtta
      661 agatcaaggt cttttcacct ggcccgcatg gacacccaga ccaggtcccc tacatcgtga
      721 cctgggaagc cttggctttt gacccccctc cctgggtcaa gccctttgta caccctaagc
      781 ctccgcctcc tcttcctcca tccgccccgt ctctccccct tgaacctcct cgttcgaccc
      841 cgcctcgatc ctccctttat ccagccctca ctccttctct aggcgccaaa cctaaacctc
      901 aagttctttc tgacagtggg gggccgctca tcgacctact tacagaagac cccccgcctt
      961 atagggaccc aagaccaccc ccttccgaca gggacggaaa tggtggagaa gcgacccctg
     1021 cgggagaggc accggacccc tccccaatgg catctcgcct acgtgggaga cgggagcccc
     1081 ctgtggccga ctccactacc tcgcaggcat tccccctccg cgcaggagga aacggacagc
     1141 ttcaatactg gccgttctcc tcttctgacc tttacaactg gaaaaataat aacccttctt
     1201 tttctgaaga tccaggtaaa ctgacagctc tgatcgagtc tgttctcatc acccatcagc
     1261 ccacctggga cgactgtcag cagctgttgg ggactctgct gaccggagaa gaaaaacaac
     1321 gggtgctctt agaggctaga aaggcggtgc ggggcgatga tgggcgcccc actcaactgc
     1381 ccaatgaagt cgatgccgct tttcccctcg agcgcccaga ctgggattac accacccagg
     1441 caggtaggaa ccacctagtc cactatcgcc agttgctcct agcgggtctc caaaacgcgg
     1501 gcagaagccc caccaatttg gccaaggtaa aaggaataac acaagggccc aatgagtctc
     1561 cctcggcctt cctagagaga cttaaggaag cctatcgcag gtacactcct tatgaccctg
     1621 aggacccagg gcaagaaact aatgtgtcta tgtctttcat ttggcagtct gccccagaca
     1681 ttgggagaaa gttagagagg ttagaagatt taaaaaacaa gacgcttgga gatttggtta
     1741 gagaggcaga aaagatcttt aataaacgag aaaccccgga agaaagagag gaacgtatca
     1801 ggagagaaac agaggaaaaa gaagaacgcc gtaggacaga ggatgagcag aaagagaaag
     1861 aaagagatcg taggagacat agagagatga gcaagctatt ggccactgtc gttagtggac
     1921 agaaacagga tagacaggga ggagaacgaa ggaggtccca actcgatcgc gaccagtgtg
     1981 cctactgcaa agaaaagggg cactgggcta aagattgtcc caagaaacca cgaggacctc
     2041 ggggaccaag accgcaaccg gtcgccaccc ctaaaaagaa acgcaaggtc gaattcggaa
     2101 gcgccgcctt caagcccaac cccatcaact acatcctggg cctggccatc ggcatcgcca
     2161 gcgtgggctg ggccatggtg gagatcgacg aggacgagaa ccccatctgc ctgatcgacc
     2221 tgggtgtgcg cgtgttcgag cgcgctgagg tgcccaagac tggtgacagt ctggctatgg
     2281 ctcgccggct tgctcgctct gttcggcgcc ttactcgccg gcgcgctcac cgccttctgc
     2341 gcgctcgccg cctgctgaag cgcgagggtg tgctgcaggc tgccgacttc gacgagaacg
     2401 gcctgatcaa gagcctgccc aacactcctt ggcagctgcg cgctgccgct ctggaccgca
     2461 agctgactcc tctggagtgg agcgccgtgc tgctgcacct gatcaagcac cgcggctacc
     2521 tgagccagcg caagaacgag ggcgagaccg ccgacaagga gctgggtgct ctgctgaagg
     2581 gcgtggccga caacgcccac gccctgcaga ctggtgactt ccgcactcct gctgagctgg
     2641 ccctgaacaa gttcgagaag gagagcggcc acatccgcaa ccagcgcggc gactacagcc
     2701 acaccttcag ccgcaaggac ctgcaggccg agctgatcct gctgttcgag aagcagaagg
     2761 agttcggcaa cccccacgtg agcggcggcc tgaaggaggg catcgagacc ctgctgatga
     2821 cccagcgccc cgccctgagc ggcgacgccg tgcagaagat gctgggccac tgcaccttcg
     2881 agccagccga gcccaaggcc gccaagaaca cctacaccgc cgagcgcttc atctggctga
     2941 ccaagctgaa caacctgcgc atcctggagc agggcagcga gcgccccctg accgacaccg
     3001 agcgcgccac cctgatggac gagccctacc gcaagagcaa gctgacctac gcccaggccc
     3061 gcaagctgct gggtctggag gacaccgcct tcttcaaggg cctgcgctac ggcaaggaca
     3121 acgccgaggc cagcaccctg atggagatga aggcctacca cgccatcagc cgcgccctgg
     3181 agaaggaggg cctgaaggac aagaagagtc ctctgaacct gagccccgag ctgcaggacg
     3241 agatcggcac cgccttcagc ctgttcaaga ccgacgagga catcaccggc cgcctgaagg
     3301 accgcatcca gcccgagatc ctggaggccc tgctgaagca catcagcttc gacaagttcg
     3361 tgcagatcag cctgaaggcc ctgcgccgca tcgtgcccct gatggagcag ggcaagcgct
     3421 acgacgaggc ctgcgccgag atctacggcg accactacgg caagaagaac accgaggaga
     3481 agatctacct gcctcctatc cccgccgacg agatccgcaa ccccgtggtg ctgcgcgccc
     3541 tgagccaggc ccgcaaggtg atcaacggcg tggtgcgccg ctacggcagc cccgcccgca
     3601 tccacatcga gaccgcccgc gaggtgggca agagcttcaa ggaccgcaag gagatcgaga
     3661 agcgccagga ggagaaccgc aaggaccgcg agaaggccgc cgccaagttc cgcgagtact
     3721 tccccaactt cgtgggcgag cccaagagca aggacatcct gaagctgcgc ctgtacgagc
     3781 agcagcacgg caagtgcctg tacagcggca aggagatcaa cctgggccgc ctgaacgaga
     3841 agggctacgt ggagatcgcc gctgccctgc ccttcagccg cacctgggac gacagcttca
     3901 acaacaaggt gctggtgctg ggcagcgagg ctcagaacaa gggcaaccag accccctacg
     3961 agtacttcaa cggcaaggac aacagccgcg agtggcagga gttcaaggcc cgcgtggaga
     4021 ccagccgctt cccccgcagc aagaagcagc gcatcctgct gcagaagttc gacgaggacg
     4081 gcttcaagga gcgcaacctg aacgacaccc gctacgtgaa ccgcttcctg tgccagttcg
     4141 tggccgaccg catgcgcctg accggcaagg gcaagaagcg cgtgttcgcc agcaacggcc
     4201 agatcaccaa cctgctgcgc ggcttctggg gcctgcgcaa ggtgcgcgcc gagaacgacc
     4261 gccaccacgc cctggacgcc gtggtggtgg cctgcagcac cgtggccatg cagcagaaga
     4321 tcacccgctt cgtgcgctac aaggagatga acgccttcga cggtaaaacc atcgacaagg
     4381 agaccggcga ggtgctgcac cagaagaccc acttccccca gccctgggag ttcttcgccc
     4441 aggaggtgat gatccgcgtg ttcggcaagc ccgacggcaa gcccgagttc gaggaggccg
     4501 acacccccga gaagctgcgc accctgctgg ccgagaagct gagcagccgc cctgaggccg
     4561 tgcacgagta cgtgactcct ctgttcgtga gccgcgcccc caaccgcaag atgagcggtc
     4621 agggtcacat ggagaccgtg aagagcgcca agcgcctgga cgagggcgtg agcgtgctgc
     4681 gcgtgcccct gacccagctg aagctgaagg acctggagaa gatggtgaac cgcgagcgcg
     4741 agcccaagct gtacgaggcc ctgaaggccc gcctggaggc ccacaaggac gaccccgcca
     4801 aggccttcgc cgagcccttc tacaagtacg acaaggccgg caaccgcacc cagcaggtga
     4861 aggccgtgcg cgtggagcag gtgcagaaga ccggcgtgtg ggtgcgcaac cacaacggca
     4921 tcgccgacaa cgccaccatg gtgcgcgtgg acgtgttcga gaagggcgac aagtactacc
     4981 tggtgcccat ctacagctgg caggtggcca agggcatcct gcccgaccgc gccgtggtgc
     5041 agggcaagga cgaggaggac tggcagctga tcgacgacag cttcaacttc aagttcagcc
     5101 tgcaccccaa cgacctggtg gaggtgatca ccaagaaggc ccgcatgttc ggctacttcg
     5161 ccagctgcca ccgcggcacc ggcaacatca acatccgcat ccacgacctg gaccacaaga
     5221 tcggcaagaa cggcatcctg gagggcatcg gcgtgaagac cgccctgagc ttccagaagt
     5281 accagatcga cgagctgggc aaggagatcc gcccctgccg cctgaagaag cgccctcctg
     5341 tgcgcggctc tactagtggc tctcccaaga agaagaggaa ggtaggatcc ggaagtatgg
     5401 tgagcaaggg cgaggaggat aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc
     5461 acatggaggg ctccgtgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc
     5521 cctacgaggg cacccagacc gccaagctga aggtgaccaa gggtggcccc ctgcccttcg
     5581 cctgggacat cctgtcccct cagttcatgt acggctccaa ggcctacgtg aagcaccccg
     5641 ccgacatccc cgactacttg aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga
     5701 tgaacttcga ggacggcggc gtggtgaccg tgacccagga ctcctcactg caggacggcg
     5761 agttcatcta caaggtgaag ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc
     5821 agaagaagac catgggctgg gaggcctcct ccgagcggat gtaccccgag gacggcgccc
     5881 tgaagggcga gatcaagcag aggctgaagc tgaaggacgg cggccactac gacgctgagg
     5941 tcaagaccac ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac aacgtcaaca
     6001 tcaagttgga catcacctcc cacaacgagg actacaccat cgtggaacag tacgaacgcg
     6061 ctgagggccg ccactccacc ggcggcatgg acgagctgta caagggtagc atcgatggta
     6121 gcatggtgag caagggcgag gaggataaca tggccatcat caaggagttc atgcgcttca
     6181 aggtgcacat ggagggctcc gtgaacggcc acgagttcga gatcgagggc gagggcgagg
     6241 gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggt ggccccctgc
     6301 ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc tacgtgaagc
     6361 accccgccga catccccgac tacttgaagc tgtccttccc cgagggcttc aagtgggagc
     6421 gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc tcactgcagg
     6481 acggcgagtt catctacaag gtgaagctgc gcggcaccaa cttcccctcc gacggccccg
     6541 taatgcagaa gaagaccatg ggctgggagg cctcctccga gcggatgtac cccgaggacg
     6601 gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa ggacggcggc cactacgacg
     6661 ctgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc gcctacaacg
     6721 tcaacatcaa gttggacatc acctcccaca acgaggacta caccatcgtg gaacagtacg
     6781 aacgcgctga gggccgccac tccaccggcg gcatggacga gctgtacaag tctggcggcc
     6841 gctctggcat ggtgagcaag ggcgaggagg ataacatggc catcatcaag gagttcatgc
     6901 gcttcaaggt gcacatggag ggctccgtga acggccacga gttcgagatc gagggcgagg
     6961 gcgagggccg cccctacgag ggcacccaga ccgccaagct gaaggtgacc aagggtggcc
     7021 ccctgccctt cgcctgggac atcctgtccc ctcagttcat gtacggctcc aaggcctacg
     7081 tgaagcaccc cgccgacatc cccgactact tgaagctgtc cttccccgag ggcttcaagt
     7141 gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac cgtgacccag gactcctcac
     7201 tgcaggacgg cgagttcatc tacaaggtga agctgcgcgg caccaacttc ccctccgacg
     7261 gccccgtaat gcagaagaag accatgggct gggaggcctc ctccgagcgg atgtaccccg
     7321 aggacggcgc cctgaagggc gagatcaagc agaggctgaa gctgaaggac ggcggccact
     7381 acgacgctga ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg cccggcgcct
     7441 acaacgtcaa catcaagttg gacatcacct cccacaacga ggactacacc atcgtggaac
     7501 agtacgaacg cgctgagggc cgccactcca ccggcggcat ggacgagctg tacaagctcg
     7561 agtagggtaa tctagataat caacctctgg attacaaaat ttgtgaaaga ttgactggta
     7621 ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc
     7681 atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt
     7741 ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg
     7801 ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt
     7861 tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct
     7921 ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt
     7981 cctttccttg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct
     8041 acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc
     8101 ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct
     8161 ccccgcctga gatcctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt
     8221 tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac aagatctgct
     8281 ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
     8341 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
     8401 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
     8461 gaaaatctct agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa
     8521 gaaatgaata tcagagagtg agaggcccgg gttaattaag gaaagggcta gatcattctt
     8581 gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg
     8641 tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
     8701 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     8761 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
     8821 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
     8881 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
     8941 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
     9001 tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca
     9061 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
     9121 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
     9181 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
     9241 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
     9301 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
     9361 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
     9421 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
     9481 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
     9541 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
     9601 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
     9661 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     9721 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     9781 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     9841 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     9901 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     9961 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
    10021 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
    10081 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
    10141 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
    10201 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
    10261 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
    10321 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
    10381 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
    10441 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
    10501 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
    10561 agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt
    10621 ggccgattca ttaatgcagc aagctcatgg ctgactaatt ttttttattt atgcagaggc
    10681 cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
    10741 aggcttttgc aaaaagctcc ccgtggcacg acaggtttcc cgactggaaa gcgggcagtg
    10801 agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta
    10861 tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca
    10921 gctatgacat gattacgaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
    10981 gtttgtccaa actcatcaat gtatcttatc atgtctggat caactggata actcaagcta
    11041 accaaaatca tcccaaactt cccaccccat accctattac cactgccaat tacctgtggt
    11101 ttcatttact ctaaacctgt gattcctctg aattattttc attttaaaga aattgtattt
    11161 gttaaatatg tactacaaac ttagtagttg gaagggctaa ttcactccca aagaagacaa
    11221 gatatccttg atctgtggat ctaccacaca caaggctact tccctgatta gcagaactac
    11281 acaccagggc caggggtcag atatccactg acctttggat ggtgctacaa gctagtacca
    11341 gttgagccag ataaggtaga agaggccaat aaaggagaga acaccagctt gttacaccct
    11401 gtgagcctgc atgggatgga tgacccggag agagaagtgt tagagtggag gtttgacagc
    11461 cgcctagcat ttcatcacgt ggcccgagag ctgcatccgg agtacttcaa gaactgctga
    11521 tatcgagctt gctacaaggg actttccgct ggggactttc cagggaggcg tggcctgggc
    11581 gggactgggg agtggcgagc cctcagatcc tgcatataag cagctgcttt ttgcctgtac
    11641 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
    11701 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
    11761 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
    11821 cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc
    11881 aggactcggc ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg
    11941 ccaaaaattt tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt
    12001 aagcggggga gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa
    12061 aaatataaat taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat
    12121 cctggcctgt tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc
    12181 cttcagacag gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt
    12241 gtgcatcaaa ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag
    12301 caaaacaaaa gtaagaccac cgcacagcaa gcggccggcc gctgatcttc agacctggag
    12361 gaggagatat gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg
    12421 aaccattagg agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag
    12481 cagtgggaat aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg
    12541 cagcgtcaat gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc
    12601 agaacaattt gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg
    12661 gcatcaagca gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc
    12721 tcctggggat ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg
    12781 ctagttggag taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg
    12841 acagagaaat taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc
    12901 agcaagaaaa gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt
    12961 ggtttaacat aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct
    13021 tggtaggttt aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat
    13081 attcaccatt atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag
    13141 gaatagaaga agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat
    13201 ctcgacggta tcgccgaatt gacaaatggc agtattcatc cacaatttta aaagaaaagg
    13261 ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca
    13321 aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga
    13381 cagcagagat ccagtttgga ctagcgatgc tagctgatgc gggccctagt tattaatagt
    13441 aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta
    13501 cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga
    13561 cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt
    13621 tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta
    13681 ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg
    13741 actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg
//
