LOCUS V020316 9788 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V020316 VERSION V020316 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers LTR 1..634 /note="3' long terminal repeat (LTR) from HIV-1" /label="3' LTR" misc_feature 681..806 /note="packaging signal of human immunodeficiency virus type 1" /label="HIV-1 Ψ" misc_feature 1303..1536 /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /label="RRE" CDS 1721..1765 /codon_start=1 /note="recognized by the 2H10 single-chain llama nanobody" /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /transl_table=1 /translation="KNEQELLELDKWASL" /label="gp41 peptide" misc_feature 2058..2175 /note="central polypurine tract and central termination sequence of HIV-1" /label="cPPT/CTS" enhancer 2227..2530 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 2531..2734 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 2853..3560 /codon_start=1 /note="mammalian codon-optimized" /product="monomeric derivative of DsRed fluorescent protein (Shaner et al., 2004)" /transl_table=1 /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA EGRHSTGGMDELYK" /label="mCherry" CDS 3576..4937 /label="Otud1(NM_027715)" /note="Otud1(NM_027715)" /gene="Otud1" CDS 4956..5027 /codon_start=1 /product="three tandem FLAG® epitope tags" /transl_table=1 /translation="DYKDDDDKDYKDDDDKDYKDDDDK" /label="3xFLAG" promoter 5045..5544 /note="mouse phosphoglycerate kinase 1 promoter" /label="PGK promoter" CDS 5565..6164 /codon_start=1 /gene="pac from Streptomyces alboniger" /note="confers resistance to puromycin" /product="puromycin N-acetyltransferase" /transl_table=1 /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*" /label="PuroR" misc_feature 6178..6766 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" LTR 6841..7074 /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" /label="3' LTR (ΔU3)" promoter 7279..7383 /gene="bla" /label="AmpR promoter" CDS 7384..8244 /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" rep_origin 8415..9003 /direction=RIGHT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" protein_bind 9414..9435 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" promoter 9450..9480 /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 9488..9504 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" primer_bind 9512..9528 /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" ORIGIN 1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg 2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa 2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca 2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtgga 2221 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg 2281 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg 2341 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca 2401 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc 2461 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc 2521 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc 2581 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa 2641 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag 2701 gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaccgtca gatcgcctgg 2761 agacgccatc cacgctgttt tgacctccat agaagacacc ggcggccacg cgtcgaagcc 2821 cgggcggatc gggtttaaac ggatccgcca ccatggtgag caagggcgag gaggataaca 2881 tggccatcat caaggagttc atgcgcttca aggtgcacat ggagggctcc gtgaacggcc 2941 acgagttcga gatcgagggc gagggcgagg gccgccccta cgagggcacc cagaccgcca 3001 agctgaaggt gaccaagggt ggccccctgc ccttcgcctg ggacatcctg tcccctcagt 3061 tcatgtacgg ctccaaggcc tacgtgaagc accccgccga catccccgac tacttgaagc 3121 tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg 3181 tgaccgtgac ccaggactcc tccctgcagg acggcgagtt catctacaag gtgaagctgc 3241 gcggcaccaa cttcccctcc gacggccccg taatgcagaa gaagaccatg ggctgggagg 3301 cctcctccga gcggatgtac cccgaggacg gcgccctgaa gggcgagatc aagcagaggc 3361 tgaagctgaa ggacggcggc cactacgacg ctgaggtcaa gaccacctac aaggccaaga 3421 agcccgtgca gctgcccggc gcctacaacg tcaacatcaa gttggacatc acctcccaca 3481 acgaggacta caccatcgtg gaacagtacg aacgcgccga gggccgccac tccaccggcg 3541 gcatggacga gctgtacaag ggcggcggag ggagtatgca gctctacagc agcgtgtgca 3601 cccattaccc tgcgggcacg ccggggccca cggccgccgc cccgcctgcc accgccgccg 3661 ccgccttcaa ggtctccttg cagtcagcca gccccgccgc cgcggccccg gagccggata 3721 ccggagagcg cccacccgcc gcggccaccg aaccacgcga agccgccgcc gccgctgcca 3781 tgcccgcctt ctctgcctgc ttcgagcggt ccgggtccgc cgccgccccg ccgggcgcgt 3841 gcagcaagcc gccgctgccg ccgcacttca cgtccacggc gcacatcgcc gtgcgggccc 3901 tgggcgccga gcgcctcctg ctgccgccac cctcagcccc ctcgccgccg cgtcgcggtt 3961 cctctgcctg gctgctggaa gagctgctcc ggcccgacga gcccgcggcc ccgaacgccg 4021 tccgcgacgc tccggacagg aacttccgac tgagcgagca ccgccaggcc ctggcagcct 4081 cccagcaccg ggcccctgca ccggcccccg tcggcccgga gcccggcgcc ggcccgggct 4141 ccggcccgtg gggtgaagag cgccgggcgg agcgcagttc ccggggttgg gaccgggcga 4201 gcggtcgcag cgacgcctcc gggtctgacg cgctccgacg gcaagatccc gaagccgagg 4261 ctcacccggt tccggcaccc gctcggagca gcggcgagcc ggcccagaac ggcgaaggcg 4321 aagccgtagg cacgtcccgg gcggatccca gggacgagaa gctagccctg tacctggccg 4381 aggtggagag gcaggacaag tacctgaggc agagaaacaa gtaccgattc cacatcatcc 4441 ccgacggcaa ctgcctctac cgagctgtca gcaagactgt gtacggggac cagagcctgc 4501 accgggagct gagggagcag accgtgcact acatcgcgga ccacctggac cacttcagcc 4561 cactcattga gggcgacgtg ggggagttta ttatcgctgc tgcccaagat ggggcgtggg 4621 ccggatatcc cgaattgctg gccatggggc agatgctgaa tgtgaatata cacttaacta 4681 ctggagggag gctggagagc cccaccgtgt ctaccatgat ccactatctg ggcccagagg 4741 attctctacg gcccagtatt tggctcagtt ggctcagcaa tggacactat gatgctgttt 4801 ttgaccactc ctatcctaac ccagagtatg acaattggtg caagcaaacc cagatacaga 4861 aaaaacgcga tgaagaactt gccaagtcca tggccatatc cctgtccaaa atgtatattg 4921 aacaaaatgc atgctctctc gagtctagag ggcccgatta caaggatgac gacgataagg 4981 attacaagga tgacgacgat aaggattaca aggatgacga cgataagtag tcgagaattc 5041 taccgggtag gggaggcgct tttcccaagg cagtctggag catgcgcttt agcagccccg 5101 ctgggcactt ggcgctacac aagtggcctc tggcctcgca cacattccac atccaccggt 5161 aggcgccaac cggctccgtt ctttggtggc cccttcgcgc caccttctac tcctccccta 5221 gtcaggaagt tcccccccgc cccgcagctc gcgtcgtgca ggacgtgaca aatggaagta 5281 gcacgtctca ctagtctcgt gcagatggac agcaccgctg agcaatggaa gcgggtaggc 5341 ctttggggca gcggccaata gcagctttgc tccttcgctt tctgggctca gaggctggga 5401 aggggtgggt ccgggggcgg gctcaggggc gggctcaggg gcggggcggg cgcccgaagg 5461 tcctccggag gcccggcatt ctgcacgctt caaaagcgca cgtctgccgc gctgttctcc 5521 tcttcctcat ctccgggcct ttcgacctgc agcccaagct taccatgacc gagtacaagc 5581 ccacggtgcg cctcgccacc cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg 5641 cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg 5701 tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg 5761 tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg 5821 cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc 5881 agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg 5941 ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc 6001 ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc 6061 gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg 6121 aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgaatcgat agatcctaat 6181 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct 6241 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg 6301 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg 6361 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt 6421 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt 6481 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg 6541 ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc 6601 tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat 6661 ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc 6721 cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcctga gatcctttaa 6781 gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac 6841 tggaagggct aattcactcc caacgaagac aagatctgct ttttgcttgt actgggtctc 6901 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 6961 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 7021 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta 7081 gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg 7141 agaggcccgg gttaattaag gaaagggcta gatcattctt gaagacgaaa gggcctcgtg 7201 atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc 7261 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat 7321 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag 7381 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt 7441 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt 7501 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc 7561 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta 7621 tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac 7681 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa 7741 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg 7801 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc 7861 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg 7921 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta 7981 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg 8041 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg 8101 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc 8161 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt 8221 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt 8281 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc 8341 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag 8401 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa 8461 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg 8521 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag 8581 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg 8641 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga 8701 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc 8761 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc 8821 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga 8881 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt 8941 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg 9001 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac 9061 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga 9121 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg 9181 gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc 9241 aagctcatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg 9301 agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc 9361 ccgtggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg 9421 agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg 9481 tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacat gattacgaat 9541 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat 9601 gtatcttatc atgtctggat caactggata actcaagcta accaaaatca tcccaaactt 9661 cccaccccat accctattac cactgccaat tacctgtggt ttcatttact ctaaacctgt 9721 gattcctctg aattattttc attttaaaga aattgtattt gttaaatatg tactacaaac 9781 ttagtagt //