LOCUS       V036101                10684 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V036101
VERSION     V036101
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             1..634
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    681..806
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1303..1536
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1721..1765
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    2058..2175
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     enhancer        2227..2530
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        2531..2734
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     regulatory      2815..2824
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             2824..2895
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags"
                     /transl_table=1
                     /translation="DYKDDDDKDYKDDDDKDYKDDDDK"
                     /label="3xFLAG"
     CDS             2929..5901
                     /label="NEK9(NM_001329237)"
                     /note="NEK9(NM_001329237)"
                     /gene="NEK9"
     promoter        5941..6440
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /label="PGK promoter"
     CDS             6461..7060
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7074..7662
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7737..7970
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     promoter        8175..8279
                     /gene="bla"
                     /label="AmpR promoter"
     CDS             8280..9140
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     rep_origin      9311..9899
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     protein_bind    10310..10331
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     promoter        10346..10376
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    10384..10400
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     primer_bind     10408..10424
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtgga
     2221 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
     2281 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
     2341 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
     2401 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
     2461 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
     2521 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
     2581 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
     2641 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
     2701 gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc agatcgcctg
     2761 gagacgccat ccacgctgtt ttgacctcca tagaagacac cggcggccac gcgtgccacc
     2821 atggattaca aggatgacga cgataaggac tataaggacg atgatgacaa ggactacaaa
     2881 gatgatgacg ataaacgaag cccgggcgga tcgggtttaa acggatccat gtcggtgctg
     2941 ggcgagtacg agcgacactg cgattccatc aactcggact ttgggagcga gtccgggggt
     3001 tgcggggact cgagtccggg gcctagcgcc agtcaggggc cgcgagccgg cggcggcgcg
     3061 gcggagcagg aggaactgca ctacatcccc atccgcgtcc tgggccgcgg cgccttcggg
     3121 gaagccacgc tgtaccgccg caccgaggat gactcactgg ttgtgtggaa ggaagtcgat
     3181 ttgacccggc tgtctgagaa ggaacgtcgt gatgccttga atgagattgt tattctggca
     3241 ctgctgcagc acgacaacat tattgcctac tacaatcact tcatggacaa taccacgctg
     3301 ctgattgagc tggaatattg taatggaggg aacctgtatg acaaaatcct tcgtcagaag
     3361 gacaagttgt ttgaggaaga gatggtggtg tggtacctat ttcagattgt ttcagcagtg
     3421 agctgcatcc ataaagctgg aatccttcat agagatataa agacattaaa tatttttctg
     3481 accaaggcaa acctgataaa acttggagat tatggcctag caaagaaact taattctgag
     3541 tattccatgg ctgagacgct tgtgggaacc ccatattaca tgtctccaga gctctgtcaa
     3601 ggagtaaagt acaatttcaa gtctgatatc tgggcagttg gctgcgtcat ttttgaactg
     3661 cttaccttaa agaggacgtt tgatgctaca aacccactta acctgtgtgt gaagatcgtg
     3721 caaggaattc gggccatgga agttgactct agccagtact ctttggaatt gatccaaatg
     3781 gttcattcgt gccttgacca ggatcctgag cagagaccta ctgcagatga acttctagat
     3841 cgccctcttc tcaggaaacg caggagagag atggaggaaa aagtcactct gcttaatgca
     3901 cctacaaaga gaccaaggtc aagcactgtg actgaagcac ccattgctgt agtaacatca
     3961 cgaaccagtg aagtctatgt ttggggtggt ggaaaatcca ccccccagaa actggatgtt
     4021 atcaagagtg gctgtagtgc ccggcaggtc tgtgcaggga atacccactt tgctgtggtc
     4081 acagtggaga aggaactgta cacttgggtg aacatgcaag gaggcactaa actccatggt
     4141 cagctgggcc atggagacaa agcctcctat cgacagccaa agcatgtgga aaagttgcaa
     4201 ggcaaagcta tccgtcaggt gtcatgtggt gatgatttca ctgtctgtgt gactgatgag
     4261 ggtcagctct atgccttcgg atcagattat tatggctgca tgggggtgga caaagttgct
     4321 ggccctgaag tgctagaacc catgcagctg aacttcttcc tcagcaatcc agtggagcag
     4381 gtctcctgtg gagataatca tgtggtggtt ctgacacgaa acaaggaagt ctattcttgg
     4441 ggctgtggcg aatatggtag gggtagcctt gtctctgtcc acttcttcac aggacgactg
     4501 ggtttggatt cagaagagga ttattataca ccacaaaagg tggatgttcc caaggccttg
     4561 attattgttg cagttcaatg tggctgtgat gggacatttc tgttgaccca gtcaggcaaa
     4621 gtgctggcct gtggactcaa tgaattcaat aagctgggtc tgaatcagtg catgtcggga
     4681 attatcaacc atgaagcata ccatgaagtt ccctacacaa cgtcctttac cttggccaaa
     4741 cagttgtcct tttataagat ccgtaccatt gccccaggca agactcacac agctgctatt
     4801 gatgagcgag gccggctgct gacctttggc tgcaacaagt gtgggcagct gggcgttggg
     4861 aactacaaga agcgtctggg aatcaacctg ttggggggac cccttggtgg gaagcaagtg
     4921 atcagggtct cctgcggtga tgagtttacc attgctgcca ctgatgataa tcacattttt
     4981 gcctggggca atggtggtaa tggccgcctg gcaatgaccc ccacagagag accacatggc
     5041 tctgatatct gtacctcatg gcctcggcct atttttggat ctctgcatca tgtcccggac
     5101 ctgtcttgcc gtggatggca taccattctc atcgttgaga aagtattgaa ttctaagacc
     5161 atccgttcca atagcagtgg cttatccatt ggaactgtgt ttcagagctc tagcccggga
     5221 ggaggcggcg ggggcggcgg tggtgaagaa gaggacagtc agcaggaatc tgaaactcct
     5281 gacccaagtg gaggcttccg aggaacaatg gaagcagacc gaggaatgga aggtttaatc
     5341 agtcccacag aggccatggg gaacagtaat ggggccagca gctcctgtcc tggctggctt
     5401 cgaaaggagc tggaaaatgc agaatttatc cccatgcctg acagcccatc tcctctcagt
     5461 gcagcgtttt cagaatctga gaaagatacc ctgccctatg aagagctgca aggactcaaa
     5521 gtggcctctg aagctccttt ggaacacaaa ccccaagtag aagcctcgtc acctcggctg
     5581 aatcctgcag taacctgtgc tgggaaggga acaccactga ctcctcctgc gtgtgcgtgc
     5641 agctctctgc aggtggaggt tgagagattg cagggtctgg tgttaaagtg tctggctgaa
     5701 caacagaagc tacagcaaga aaacctccag atttttaccc aactgcagaa gttgaacaag
     5761 aaattagaag gagggcagca ggtggggatg cattccaaag gaactcagac agcaaaggaa
     5821 gagatggaaa tggatccaaa gcctgactta gattcagatt cctggtgcct cctgggaaca
     5881 gactcctgta gacccagcct ctagctcgag tctagagggc ccttagtcga gaattctacc
     5941 gggtagggga ggcgcttttc ccaaggcagt ctggagcatg cgctttagca gccccgctgg
     6001 gcacttggcg ctacacaagt ggcctctggc ctcgcacaca ttccacatcc accggtaggc
     6061 gccaaccggc tccgttcttt ggtggcccct tcgcgccacc ttctactcct cccctagtca
     6121 ggaagttccc ccccgccccg cagctcgcgt cgtgcaggac gtgacaaatg gaagtagcac
     6181 gtctcactag tctcgtgcag atggacagca ccgctgagca atggaagcgg gtaggccttt
     6241 ggggcagcgg ccaatagcag ctttgctcct tcgctttctg ggctcagagg ctgggaaggg
     6301 gtgggtccgg gggcgggctc aggggcgggc tcaggggcgg ggcgggcgcc cgaaggtcct
     6361 ccggaggccc ggcattctgc acgcttcaaa agcgcacgtc tgccgcgctg ttctcctctt
     6421 cctcatctcc gggcctttcg acctgcagcc caagcttacc atgaccgagt acaagcccac
     6481 ggtgcgcctc gccacccgcg acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt
     6541 cgccgactac cccgccacgc gccacaccgt cgatccggac cgccacatcg agcgggtcac
     6601 cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc
     6661 ggacgacggc gccgcggtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt
     6721 gttcgccgag atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca
     6781 acagatggaa ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac
     6841 cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg
     6901 agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa
     6961 cctccccttc tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg
     7021 accgcgcacc tggtgcatga cccgcaagcc cggtgcctga atcgatagat cctaatcaac
     7081 ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta
     7141 cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt
     7201 tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg
     7261 ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg
     7321 gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca
     7381 cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca
     7441 ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg ctcgcctgtg
     7501 ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag
     7561 cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc
     7621 gccctcagac gagtcggatc tccctttggg ccgcctcccc gcctgagatc ctttaagacc
     7681 aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga
     7741 agggctaatt cactcccaac gaagacaaga tctgcttttt gcttgtactg ggtctctctg
     7801 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     7861 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     7921 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc
     7981 atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag
     8041 gcccgggtta attaaggaaa gggctagatc attcttgaag acgaaagggc ctcgtgatac
     8101 gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt
     8161 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
     8221 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
     8281 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
     8341 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
     8401 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
     8461 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
     8521 gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
     8581 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
     8641 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
     8701 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
     8761 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
     8821 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
     8881 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
     8941 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
     9001 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
     9061 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
     9121 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
     9181 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     9241 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     9301 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     9361 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     9421 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
     9481 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     9541 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     9601 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     9661 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     9721 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     9781 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     9841 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     9901 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     9961 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
    10021 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
    10081 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagcaagc
    10141 tcatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct
    10201 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctccccgt
    10261 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt
    10321 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg
    10381 gaattgtgag cggataacaa tttcacacag gaaacagcta tgacatgatt acgaatttca
    10441 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
    10501 cttatcatgt ctggatcaac tggataactc aagctaacca aaatcatccc aaacttccca
    10561 ccccataccc tattaccact gccaattacc tgtggtttca tttactctaa acctgtgatt
    10621 cctctgaatt attttcattt taaagaaatt gtatttgtta aatatgtact acaaacttag
    10681 tagt
//
