LOCUS       V014822                 8011 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V014822
VERSION     V014822
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8011)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..8011
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             364..1386
                     /label="HygR"
                     /note="aminoglycoside phosphotransferase from E. coli"
     misc_feature    1403..1991
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     LTR             2066..2299
                     /label="3' LTR (Delta-U3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        2504..2608
                     /label="AmpR promoter"
     CDS             2609..3466
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      3640..4228
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    4639..4660
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        4675..4705
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    4713..4729
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4737..4753
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     LTR             5014..5647
                     /label="3' LTR"
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    5694..5819
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    6316..6549
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             6734..6778
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             6927..6968
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     misc_feature    7071..7188
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     enhancer        7240..7543
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7544..7747
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     promoter        7855..8011
                     /label="PGK promoter"
                     /note="mouse phosphoglycerate kinase 1 promoter"
ORIGIN
        1 accttctact cctcccctag tcaggaagtt cccccccgcc ccgcagctcg cgtcgtgcag
       61 gacgtgacaa atggaagtag cacgtctcac tagtctcgtg cagatggaca gcaccgctga
      121 gcaatggaag cgggtaggcc tttggggcag cggccaatag cagctttgct ccttcgcttt
      181 ctgggctcag aggctgggaa ggggtgggtc cgggggcggg ctcaggggcg ggctcagggg
      241 cggggcgggc gcccgaaggt cctccggagg cccggcattc tgcacgcttc aaaagcgcac
      301 gtctgccgcg ctgttctcct cttcctcatc tccgggcctt tcgacctgca gcccaagctt
      361 accatgaaaa agcctgaact caccgcgacg tctgtcgaga agtttctgat cgaaaagttc
      421 gacagcgtct ccgacctgat gcagctctcg gagggcgaag aatctcgtgc tttcagcttc
      481 gatgtaggag ggcgtggata tgtcctgcgg gtaaatagct gcgccgatgg tttctacaaa
      541 gatcgttatg tttatcggca ctttgcatcg gccgcgctcc cgattccgga agtgcttgac
      601 attggggaat tcagcgagag cctgacctat tgcatctccc gccgtgcaca gggtgtcacg
      661 ttgcaagacc tgcctgaaac cgaactgccc gctgttctgc agccggtcgc ggaggccatg
      721 gatgcgatcg ctgcggccga tcttagccag acgagcgggt tcggcccatt cggaccgcaa
      781 ggaatcggtc aatacactac atggcgtgat ttcatatgcg cgattgctga tccccatgtg
      841 tatcactggc aaactgtgat ggacgacacc gtcagtgcgt ccgtcgcgca ggctctcgat
      901 gagctgatgc tttgggccga ggactgcccc gaagtccggc acctcgtgca cgcggatttc
      961 ggctccaaca atgtcctgac ggacaatggc cgcataacag cggtcattga ctggagcgag
     1021 gcgatgttcg gggattccca atacgaggtc gccaacatct tcttctggag gccgtggttg
     1081 gcttgtatgg agcagcagac gcgctacttc gagcggaggc atccggagct tgcaggatcg
     1141 ccgcggctcc gggcgtatat gctccgcatt ggtcttgacc aactctatca gagcttggtt
     1201 gacggcaatt tcgatgatgc agcttgggcg cagggtcgat gcgacgcaat cgtccgatcc
     1261 ggagccggga ctgtcgggcg tacacaaatc gcccgcagaa gcgcggccgt ctggaccgat
     1321 ggctgtgtag aagtactcgc cgatagtgga aaccgacgcc ccagcactcg tccgagggca
     1381 aaggaatgaa tcgatagatc ctaatcaacc tctggattac aaaatttgtg aaagattgac
     1441 tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt
     1501 gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt
     1561 gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt
     1621 gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg
     1681 gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg
     1741 ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc
     1801 atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt
     1861 ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc
     1921 tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc
     1981 cgcctccccg cctgagatcc tttaagacca atgacttaca aggcagctgt agatcttagc
     2041 cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat
     2101 ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct
     2161 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta
     2221 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca
     2281 gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta tttataactt
     2341 gcaaagaaat gaatatcaga gagtgagagg cccgggttaa ttaaggaaag ggctagatca
     2401 ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat
     2461 aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
     2521 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     2581 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
     2641 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
     2701 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
     2761 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
     2821 agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg
     2881 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
     2941 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
     3001 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
     3061 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
     3121 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
     3181 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
     3241 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
     3301 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
     3361 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     3421 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     3481 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     3541 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     3601 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     3661 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     3721 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     3781 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     3841 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     3901 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     3961 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     4021 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     4081 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     4141 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     4201 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     4261 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     4321 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     4381 cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc
     4441 gcgttggccg attcattaat gcagcaagct catggctgac taattttttt tatttatgca
     4501 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga
     4561 ggcctaggct tttgcaaaaa gctccccgtg gcacgacagg tttcccgact ggaaagcggg
     4621 cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca
     4681 ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg
     4741 aaacagctat gacatgatta cgaatttcac aaataaagca tttttttcac tgcattctag
     4801 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatcaact ggataactca
     4861 agctaaccaa aatcatccca aacttcccac cccataccct attaccactg ccaattacct
     4921 gtggtttcat ttactctaaa cctgtgattc ctctgaatta ttttcatttt aaagaaattg
     4981 tatttgttaa atatgtacta caaacttagt agttggaagg gctaattcac tcccaaagaa
     5041 gacaagatat ccttgatctg tggatctacc acacacaagg ctacttccct gattagcaga
     5101 actacacacc agggccaggg gtcagatatc cactgacctt tggatggtgc tacaagctag
     5161 taccagttga gccagataag gtagaagagg ccaataaagg agagaacacc agcttgttac
     5221 accctgtgag cctgcatggg atggatgacc cggagagaga agtgttagag tggaggtttg
     5281 acagccgcct agcatttcat cacgtggccc gagagctgca tccggagtac ttcaagaact
     5341 gctgatatcg agcttgctac aagggacttt ccgctgggga ctttccaggg aggcgtggcc
     5401 tgggcgggac tggggagtgg cgagccctca gatcctgcat ataagcagct gctttttgcc
     5461 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
     5521 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
     5581 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
     5641 tctagcagtg gcgcccgaac agggacttga aagcgaaagg gaaaccagag gagctctctc
     5701 gacgcaggac tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg cgactggtga
     5761 gtacgccaaa aattttgact agcggaggct agaaggagag agatgggtgc gagagcgtca
     5821 gtattaagcg ggggagaatt agatcgcgat gggaaaaaat tcggttaagg ccagggggaa
     5881 agaaaaaata taaattaaaa catatagtat gggcaagcag ggagctagaa cgattcgcag
     5941 ttaatcctgg cctgttagaa acatcagaag gctgtagaca aatactggga cagctacaac
     6001 catcccttca gacaggatca gaagaactta gatcattata taatacagta gcaaccctct
     6061 attgtgtgca tcaaaggata gagataaaag acaccaagga agctttagac aagatagagg
     6121 aagagcaaaa caaaagtaag accaccgcac agcaagcggc cggccgctga tcttcagacc
     6181 tggaggagga gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa
     6241 aattgaacca ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa
     6301 aagagcagtg ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat
     6361 gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca
     6421 gcagcagaac aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt
     6481 ctggggcatc aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca
     6541 acagctcctg gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg
     6601 gaatgctagt tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga
     6661 gtgggacaga gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca
     6721 aaaccagcaa gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg
     6781 gaattggttt aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg
     6841 aggcttggta ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca
     6901 gggatattca ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc
     6961 cgaaggaata gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa
     7021 cggatctcga cggtatcgcc gaattcacaa atggcagtat tcatccacaa ttttaaaaga
     7081 aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac
     7141 atacaaacta aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac
     7201 agggacagca gagatccagt ttggactagt ggagttccgc gttacataac ttacggtaaa
     7261 tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt
     7321 tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta
     7381 aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt
     7441 caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc
     7501 tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca
     7561 gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat
     7621 tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa
     7681 caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag
     7741 cagagctcgt ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct
     7801 ccatagaaga caccggcggc cgcaccggtc tgcagctagc tcgagaattc taccgggtag
     7861 gggaggcgct tttcccaagg cagtctggag catgcgcttt agcagccccg ctgggcactt
     7921 ggcgctacac aagtggcctc tggcctcgca cacattccac atccaccggt aggcgccaac
     7981 cggctccgtt ctttggtggc cccttcgcgc c
//
