LOCUS       V013902                 9975 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V013902
VERSION     V013902
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9975)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..9975
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label="3' LTR"
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1303..1536
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             1721..1765
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             1914..1955
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     misc_feature    2058..2175
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     promoter        2224..3405
                     /label="EF-1-alpha promoter"
                     /note="strong constitutive promoter for human elongation
                     factor EF-1-alpha"
     CDS             3419..5068
                     /label="luciferase"
                     /note="firefly luciferase"
     misc_feature    5074..5652
                     /label="IRES2"
                     /note="internal ribosome entry site (IRES) of the
                     encephalomyocarditis virus (EMCV)"
     CDS             5656..6348
                     /label="ZsGreen1"
                     /note="Zoanthus green fluorescent protein"
     misc_feature    6365..6953
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     LTR             7028..7261
                     /label="3' LTR (Delta-U3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        7466..7570
                     /label="AmpR promoter"
     CDS             7571..8428
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      8602..9190
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    9601..9622
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        9637..9667
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt
     2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
     2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
     2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
     2521 ttacttccac gcccctggct gcagtacgtg attcttgatc ccgagcttcg ggttggaagt
     2581 gggtgggaga gttcgaggcc ttgcgcttaa ggagcccctt cgcctcgtgc ttgagttgag
     2641 gcctggcctg ggcgctgggg ccgccgcgtg cgaatctggt ggcaccttcg cgcctgtctc
     2701 gctgctttcg ataagtctct agccatttaa aatttttgat gacctgctgc gacgcttttt
     2761 ttctggcaag atagtcttgt aaatgcgggc caagatctgc acactggtat ttcggttttt
     2821 ggggccgcgg gcggcgacgg ggcccgtgcg tcccagcgca catgttcggc gaggcggggc
     2881 ctgcgagcgc ggccaccgag aatcggacgg gggtagtctc aagctggccg gcctgctctg
     2941 gtgcctggcc tcgcgccgcc gtgtatcgcc ccgccctggg cggcaaggct ggcccggtcg
     3001 gcaccagttg cgtgagcgga aagatggccg cttcccggcc ctgctgcagg gagctcaaaa
     3061 tggaggacgc ggcgctcggg agagcgggcg ggtgagtcac ccacacaaag gaaaagggcc
     3121 tttccgtcct cagccgtcgc ttcatgtgac tccacggagt accgggcgcc gtccaggcac
     3181 ctcgattagt tctcgagctt ttggagtacg tcgtctttag gttgggggga ggggttttat
     3241 gcgatggagt ttccccacac tgagtgggtg gagactgaag ttaggccagc ttggcacttg
     3301 atgtaattct ccttggaatt tgcccttttt gagtttggat cttggttcat tctcaagcct
     3361 cagacagtgg ttcaaagttt ttttcttcca tttcaggtgt cgtgaagcgg ccgccaccat
     3421 ggaagatgcc aaaaacatta agaagggccc agcgccattc tacccactcg aagacgggac
     3481 cgccggcgag cagctgcaca aagccatgaa gcgctacgcc ctggtgcccg gcaccatcgc
     3541 ctttaccgac gcacatatcg aggtggacat tacctacgcc gagtacttcg agatgagcgt
     3601 tcggctggca gaagctatga agcgctatgg gctgaataca aaccatcgga tcgtggtgtg
     3661 cagcgagaat agcttgcagt tcttcatgcc cgtgttgggt gccctgttca tcggtgtggc
     3721 tgtggcccca gctaacgaca tctacaacga gcgcgagctg ctgaacagca tgggcatcag
     3781 ccagcccacc gtcgtattcg tgagcaagaa agggctgcaa aagatcctca acgtgcaaaa
     3841 gaagctaccg atcatacaaa agatcatcat catggatagc aagaccgact accagggctt
     3901 ccaaagcatg tacaccttcg tgacttccca tttgccaccc ggcttcaacg agtacgactt
     3961 cgtgcccgag agcttcgacc gggacaaaac catcgccctg atcatgaaca gtagtggcag
     4021 taccggattg cccaagggcg tagccctacc gcaccgcacc gcttgtgtcc gattcagtca
     4081 tgcccgcgac cccatcttcg gcaaccagat catccccgac accgctatcc tcagcgtggt
     4141 gccatttcac cacggcttcg gcatgttcac cacgctgggc tacttgatct gcggctttcg
     4201 ggtcgtgctc atgtaccgct tcgaggagga gctattcttg cgcagcttgc aagactataa
     4261 gattcaatct gccctgctgg tgcccacact atttagcttc ttcgctaaga gcactctcat
     4321 cgacaagtac gacctaagca acttgcacga gatcgccagc ggcggggcgc cgctcagcaa
     4381 ggaggtaggt gaggccgtgg ccaaacgctt ccacctacca ggcatccgcc agggctacgg
     4441 cctgacagaa acaaccagcg ccattctgat cacccccgaa ggggacgaca agcctggcgc
     4501 agtaggcaag gtggtgccct tcttcgaggc taaggtggtg gacttggaca ccggtaagac
     4561 actgggtgtg aaccagcgcg gcgagctgtg cgtccgtggc cccatgatca tgagcggcta
     4621 cgttaacaac cccgaggcta caaacgctct catcgacaag gacggctggc tgcacagcgg
     4681 cgacatcgcc tactgggacg aggacgagca cttcttcatc gtggaccggc tgaagagcct
     4741 gatcaaatac aagggctacc aggtagcccc agccgaactg gagagcatcc tgctgcaaca
     4801 ccccaacatc ttcgacgccg gggtcgccgg cctgcccgac gacgatgccg gcgagctgcc
     4861 cgccgcagtc gtcgtgctgg aacacggtaa aaccatgacc gagaaggaga tcgtggacta
     4921 tgtggccagc caggttacaa ccgccaagaa gctgcgcggt ggtgttgtgt tcgtggacga
     4981 ggtgcctaaa ggactgaccg gcaagttgga cgcccgcaag atccgcgaga ttctcattaa
     5041 ggccaagaag ggcggcaaga tcgccgtgta aggatccctc ccccccccct aacgttactg
     5101 gccgaagccg cttggaataa ggccggtgtg cgtttgtcta tatgttattt tccaccatat
     5161 tgccgtcttt tggcaatgtg agggcccgga aacctggccc tgtcttcttg acgagcattc
     5221 ctaggggtct ttcccctctc gccaaaggaa tgcaaggtct gttgaatgtc gtgaaggaag
     5281 cagttcctct ggaagcttct tgaagacaaa caacgtctgt agcgaccctt tgcaggcagc
     5341 ggaacccccc acctggcgac aggtgcctct gcggccaaaa gccacgtgta taagatacac
     5401 ctgcaaaggc ggcacaaccc cagtgccacg ttgtgagttg gatagttgtg gaaagagtca
     5461 aatggctctc ctcaagcgta ttcaacaagg ggctgaagga tgcccagaag gtaccccatt
     5521 gtatgggatc tgatctgggg cctcggtgca catgctttac atgtgtttag tcgaggttaa
     5581 aaaaacgtct aggccccccg aaccacgggg acgtggtttt cctttgaaaa acacgatgat
     5641 aatatggcca cacatatggc ccagtccaag cacggcctga ccaaggagat gaccatgaag
     5701 taccgcatgg agggctgcgt ggacggccac aagttcgtga tcaccggcga gggcatcggc
     5761 taccccttca agggcaagca ggccatcaac ctgtgcgtgg tggagggcgg ccccttgccc
     5821 ttcgccgagg acatcttgtc cgccgccttc atgtacggca accgcgtgtt caccgagtac
     5881 ccccaggaca tcgtcgacta cttcaagaac tcctgccccg ccggctacac ctgggaccgc
     5941 tccttcctgt tcgaggacgg cgccgtgtgc atctgcaacg ccgacatcac cgtgagcgtg
     6001 gaggagaact gcatgtacca cgagtccaag ttctacggcg tgaacttccc cgccgacggc
     6061 cccgtgatga agaagatgac cgacaactgg gagccctcct gcgagaagat catccccgtg
     6121 cccaagcagg gcatcttgaa gggcgacgtg agcatgtacc tgctgctgaa ggacggtggc
     6181 cgcttgcgct gccagttcga caccgtgtac aaggccaagt ccgtgccccg caagatgccc
     6241 gactggcact tcatccagca caagctgacc cgcgaggacc gcagcgacgc caagaaccag
     6301 aagtggcacc tgaccgagca cgccatcgcc tccggctccg ccttgccctg aatcgataga
     6361 tcctaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt
     6421 tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc
     6481 ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga
     6541 gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc
     6601 cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct
     6661 ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg
     6721 gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct
     6781 gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc
     6841 cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg
     6901 tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcctgagat
     6961 cctttaagac caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag
     7021 gggggactgg aagggctaat tcactcccaa cgaagacaag atctgctttt tgcttgtact
     7081 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
     7141 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
     7201 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
     7261 agtagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca
     7321 gagagtgaga ggcccgggtt aattaaggaa agggctagat cattcttgaa gacgaaaggg
     7381 cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc
     7441 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     7501 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     7561 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
     7621 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
     7681 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
     7741 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
     7801 ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
     7861 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
     7921 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
     7981 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
     8041 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
     8101 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
     8161 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
     8221 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
     8281 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
     8341 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
     8401 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     8461 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     8521 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     8581 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     8641 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     8701 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     8761 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     8821 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     8881 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     8941 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     9001 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     9061 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     9121 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     9181 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     9241 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     9301 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
     9361 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
     9421 atgcagcaag ctcatggctg actaattttt tttatttatg cagaggccga ggccgcctcg
     9481 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa
     9541 aagctccccg tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat
     9601 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
     9661 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgacatgat
     9721 tacgaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
     9781 catcaatgta tcttatcatg tctggatcaa ctggataact caagctaacc aaaatcatcc
     9841 caaacttccc accccatacc ctattaccac tgccaattac ctgtggtttc atttactcta
     9901 aacctgtgat tcctctgaat tattttcatt ttaaagaaat tgtatttgtt aaatatgtac
     9961 tacaaactta gtagt
//
