LOCUS       pH6HTN_His6HaloT        4014 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Vector with a traditional MCS, for bacterial or cell-free expression
            of a protein with a cleavable N-terminal 6xHis-HaloTag(R) dual tag.
ACCESSION   .
VERSION     .
KEYWORDS    pH6HTN His6HaloTag T7
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4014)
  AUTHORS   Promega
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 4014)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4014
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        21..39
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             70..72
                     /codon_start=1
                     /product="start codon"
                     /label=start codon
                     /note="ATG"
                     /translation="M"
     CDS             76..93
                     /label=6xHis
                     /note="6xHis affinity tag"
     CDS             94..981
                     /label=HaloTag(R)
                     /note="modified bacterial dehalogenase that forms covalent
                     bonds with chloroalkane derivatives"
     CDS             994..1014
                     /label=TEV site
                     /note="tobacco etch virus (TEV) protease recognition and 
                     cleavage site"
     misc_feature    1021..1096
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     terminator      1132..1179
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        1408..1512
                     /label=AmpR promoter
     CDS             1513..2370
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      complement(2531..3119)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    3235..3518
                     /label=cer region
                     /note="ColE1-derived recombination site that helps to
                     maintain plasmids as monomers"
     terminator      3748..3834
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      3926..3953
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
ORIGIN
        1 ggatctcgat cccgcgaaat taatacgact cactataggg tattttaact ttactaagga
       61 gaattcatca tgaaacatca tcaccatcac cacgcagaaa tcggtactgg ctttccattc
      121 gacccccatt atgtggaagt cctgggcgag cgcatgcact acgtcgatgt tggtccgcgc
      181 gatggcaccc ctgtgctgtt cctgcacggt aacccgacct cctcctacgt gtggcgcaac
      241 atcatcccgc atgttgcacc gacccatcgc tgcattgctc cagacctgat cggtatgggc
      301 aaatccgaca aaccagacct gggttatttc ttcgacgacc acgtccgctt catggatgcc
      361 ttcatcgaag ccctgggtct ggaagaggtc gtcctggtca ttcacgactg gggctccgct
      421 ctgggtttcc actgggccaa gcgcaatcca gagcgcgtca aaggtattgc atttatggag
      481 ttcatccgcc ctatcccgac ctgggacgaa tggccagaat ttgcccgcga gaccttccag
      541 gccttccgca ccaccgacgt cggccgcaag ctgatcatcg atcagaacgt ttttatcgag
      601 ggtacgctgc cgatgggtgt cgtccgcccg ctgactgaag tcgagatgga ccattaccgc
      661 gagccgttcc tgaatcctgt tgaccgcgag ccactgtggc gcttcccaaa cgagctgcca
      721 atcgccggtg agccagcgaa catcgtcgcg ctggtcgaag aatacatgga ctggctgcac
      781 cagtcccctg tcccgaagct gctgttctgg ggcaccccag gcgttctgat cccaccggcc
      841 gaagccgctc gcctggccaa aagcctgcct aactgcaagg ctgtggacat cggcccgggt
      901 ctgaatctgc tgcaagaaga caacccggac ctgatcggca gcgagatcgc gcgctggctg
      961 tcgacgctcg agatttccgg cgagccaacc actgaggatc tgtactttca gagcgataac
     1021 gcgatcgctt ccgaattcag agctcaaccg cggatatcta gatttgggcc caattcctgc
     1081 aggattttgc ggccgcttgc tgagttggct gctgccaccg ctgagcaata actagcataa
     1141 ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc
     1201 ggttcgcttg ctgtccataa aaccgcccag tctagctatc gccatgtaag cccactgcaa
     1261 gctacctgct ttctctttgc gcttgcgttt tcccttgtcc agatagccca gtagctgaca
     1321 ttcatccggg gtcagcaccg tttctgcgga ctggctttct acgtaatggt ttcttagacg
     1381 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     1441 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     1501 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
     1561 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
     1621 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
     1681 agttttcgcc ccgaagaacg ttttccaatg atgagcactt tcaaagttct gctatgtggc
     1741 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct
     1801 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
     1861 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
     1921 ctgacaacta tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
     1981 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
     2041 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
     2101 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
     2161 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
     2221 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
     2281 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
     2341 gagataggtg cctcactgat taagcattgg taattcgaaa tgaccgacca agcgacgccc
     2401 aaccggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     2461 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     2521 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     2581 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     2641 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     2701 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     2761 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     2821 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     2881 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     2941 agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga
     3001 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     3061 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatttcaag
     3121 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     3181 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta tagtccggaa
     3241 atacaggaac gcacgctgga tggcccttcg ctgggatggt gaaaccatga aaaatggcag
     3301 cttcagtgga ttaagtgggg gtaatgtggc ctgtaccctc tggttgcata ggtattcata
     3361 cggttaaaat ttatcaggcg cgattgcggc agtttttcgg gtggtttgtt gccattttta
     3421 cctgtctgct gccgtgatcg cgctgaacgc gttttagcgg tgcgtacaat taagggatta
     3481 tggtaaatcc acttactgtc tgccctcgta gccatcgaga taaaccgcag tactccggcc
     3541 acgatgcgtc cggcgtagag gatcgagatc ttttcagcct gatacagatt aaatcagaac
     3601 gcagaagcgg tctgataaaa cagaatttgc ctggcggcag tagcgcggtg gtcccacctg
     3661 accccatgcc gaactcagaa gtgaaacgcc gtagcgccga tggtagtgtg gggtctcccc
     3721 atgcgagagt agggaactgc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg
     3781 gcctttcgtt ttatctgttg tttgtcggtg aacgctctcc tgagtaggac aaatccgccg
     3841 ggagcggatt tgaacgttgc gaagcaacgg cccggagggt ggcgggcagg acgcccgcca
     3901 taaactgcca ggcatcaaat taagcagaag gccatcctga cggatggcct ttttgcgttt
     3961 ctacaaactc ttttgtttat ttttctaaat acattcaaat atgtatccgc tcat
//
