LOCUS       pH6HTC_His6HaloT        3984 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Vector with a traditional MCS, for bacterial or cell-free expression
            of a protein with a cleavable C-terminal HaloTag(R)-6xHis dual tag.
ACCESSION   .
VERSION     .
KEYWORDS    pH6HTC His6HaloTag T7
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3984)
  AUTHORS   Promega
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 3984)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3984
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        21..39
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    61..129
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     CDS             139..159
                     /label=TEV site
                     /note="tobacco etch virus (TEV) protease recognition and 
                     cleavage site"
     CDS             172..1059
                     /label=HaloTag(R)
                     /note="modified bacterial dehalogenase that forms covalent
                     bonds with chloroalkane derivatives"
     CDS             1060..1077
                     /label=6xHis
                     /note="6xHis affinity tag"
     terminator      1102..1149
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        1378..1482
                     /label=AmpR promoter
     CDS             1483..2340
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      complement(2501..3089)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    3205..3488
                     /label=cer region
                     /note="ColE1-derived recombination site that helps to
                     maintain plasmids as monomers"
     terminator      3718..3804
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      3896..3923
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
ORIGIN
        1 ggatctcgat cccgcgaaat taatacgact cactataggg tattttaact ttactaagga
       61 gcgatcgctt ccgaattcct accgcggata tctagatttg ggcccaattc ctgcaggcga
      121 gctctcgagc caaccactga ggatctgtac tttcagagcg ataacgatgg atccgaaatc
      181 ggtactggct ttccattcga cccccattat gtggaagtcc tgggcgagcg catgcactac
      241 gtcgatgttg gtccgcgcga tggcacccct gtgctgttcc tgcacggtaa cccgacctcc
      301 tcctacgtgt ggcgcaacat catcccgcat gttgcaccga cccatcgctg cattgctcca
      361 gacctgatcg gtatgggcaa atccgacaaa ccagacctgg gttatttctt cgacgaccac
      421 gtccgcttca tggatgcctt catcgaagcc ctgggtctgg aagaggtcgt cctggtcatt
      481 cacgactggg gctccgctct gggtttccac tgggccaagc gcaatccaga gcgcgtcaaa
      541 ggtattgcat ttatggagtt catccgccct atcccgacct gggacgaatg gccagaattt
      601 gcccgcgaga ccttccaggc cttccgcacc accgacgtcg gccgcaagct gatcatcgat
      661 cagaacgttt ttatcgaggg tacgctgccg atgggtgtcg tccgcccgct gactgaagtc
      721 gagatggacc attaccgcga gccgttcctg aatcctgttg accgcgagcc actgtggcgc
      781 ttcccaaacg agctgccaat cgccggtgag ccagcgaaca tcgtcgcgct ggtcgaagaa
      841 tacatggact ggctgcacca gtcccctgtc ccgaagctgc tgttctgggg caccccaggc
      901 gttctgatcc caccggccga agccgctcgc ctggccaaaa gcctgcctaa ctgcaaggct
      961 gtggacatcg gcccgggtct gaatctgctg caagaagaca acccggacct gatcggcagc
     1021 gagatcgcgc gctggctgtc tactctggag atttccggtc atcatcacca tcaccactaa
     1081 tagtcaaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg
     1141 ggttttttgc tgaaaggagg aactatatcc ggttcgcttg ctgtccataa aaccgcccag
     1201 tctagctatc gccatgtaag cccactgcaa gctacctgct ttctctttgc gcttgcgttt
     1261 tcccttgtcc agatagccca gtagctgaca ttcatccggg gtcagcaccg tttctgcgga
     1321 ctggctttct acgtaatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc
     1381 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     1441 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
     1501 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
     1561 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
     1621 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
     1681 atgagcactt tcaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
     1741 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
     1801 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
     1861 atgagtgata acactgcggc caacttactt ctgacaacta tcggaggacc gaaggagcta
     1921 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
     1981 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
     2041 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
     2101 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
     2161 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
     2221 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
     2281 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
     2341 taattcgaaa tgaccgacca agcgacgccc aaccggtatc agctcactca aaggcggtaa
     2401 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     2461 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     2521 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     2581 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     2641 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     2701 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     2761 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     2821 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     2881 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     2941 ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     3001 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     3061 agattacgcg cagaaaaaaa ggatttcaag aagatccttt gatcttttct acggggtctg
     3121 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     3181 tcttcaccta gatcctttta tagtccggaa atacaggaac gcacgctgga tggcccttcg
     3241 ctgggatggt gaaaccatga aaaatggcag cttcagtgga ttaagtgggg gtaatgtggc
     3301 ctgtaccctc tggttgcata ggtattcata cggttaaaat ttatcaggcg cgattgcggc
     3361 agtttttcgg gtggtttgtt gccattttta cctgtctgct gccgtgatcg cgctgaacgc
     3421 gttttagcgg tgcgtacaat taagggatta tggtaaatcc acttactgtc tgccctcgta
     3481 gccatcgaga taaaccgcag tactccggcc acgatgcgtc cggcgtagag gatcgagatc
     3541 ttttcagcct gatacagatt aaatcagaac gcagaagcgg tctgataaaa cagaatttgc
     3601 ctggcggcag tagcgcggtg gtcccacctg accccatgcc gaactcagaa gtgaaacgcc
     3661 gtagcgccga tggtagtgtg gggtctcccc atgcgagagt agggaactgc caggcatcaa
     3721 ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg
     3781 aacgctctcc tgagtaggac aaatccgccg ggagcggatt tgaacgttgc gaagcaacgg
     3841 cccggagggt ggcgggcagg acgcccgcca taaactgcca ggcatcaaat taagcagaag
     3901 gccatcctga cggatggcct ttttgcgttt ctacaaactc ttttgtttat ttttctaaat
     3961 acattcaaat atgtatccgc tcat
//
