LOCUS       62056_11985        4742 bp DNA     circular SYN 07-NOV-2020
DEFINITION  Cloning vector pH-iGFP, complete sequence.
ACCESSION   MT219956
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4742)
  AUTHORS   Riedl A, Ruzsics Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAR-2020) Institute of Virology, University Medical 
            Center Freiburg, Hermann-Herder-Str. 11, Freiburg, BW 79104, Germany
REFERENCE   2  (bases 1 to 4742)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (18-MAR-2020) Institute of Virology, University Medical Center 
            Freiburg, Hermann-Herder-Str. 11, Freiburg, BW 79104, Germany"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4742
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      496..718
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     CDS             766..1713
                     /codon_start=1
                     /label=Rep101(Ts)
                     /note="temperature-sensitive version of the RepA protein
                     needed for replication with the pSC101 origin (Armstrong et
                     al., 1984)"
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYVQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMELVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     misc_feature    1786..2372
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     CDS             2373..3089
                     /codon_start=1
                     /label=EGFP
                     /note="enhanced GFP"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     polyA_signal    3215..3336
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        3375..3479
                     /label=AmpR promoter
     CDS             3480..4337
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERSPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 gtacgtccgg agtcgagaca gtaagacggg taagcctgtt gatgataccg ctgccttact
       61 gggtgcatta gccagtctga atgacctgtc acgggataat ccgaagtggt cagactggaa
      121 aatcagaggg caggaactgc tgaacagcaa aaagtcagat aacaccacat agcagacccg
      181 ccataaaacg ccctgagaag cccgtgacgg gcttttcttg tattatgggt agtttccttg
      241 catgaatcca taaaaggcgc ctgtagtgcc atttaccccc attcactgcc agagccgtga
      301 gcgcagcgaa ctgaatgtca cgaaaaagac agcgactcag gtgcctgatg gtcggagaca
      361 aaaggaatat tcagcgattt gcccgagctt gcgagggtgc tacttaagcc tttagggttt
      421 taaggtctgt tttgtagagg agcaaacagc gtttgcgaca tccttttgta atactgcgga
      481 actgactaaa gtagtgagtt atacacaggg ctgggatcta ttctttttat ctttttttat
      541 tctttcttta ttctataaat tataaccact tgaatataaa caaaaaaaac acacaaaggt
      601 ctagcggaat ttacagaggg tctagcagaa tttacaagtt ttccagcaaa ggtctagcag
      661 aatttacaga tacccacaac tcaaaggaaa aggactagta attatcattg actagcccat
      721 ctcaattggt atagtgatta aaatcaccta gaccaattga gatgtatgtc tgaattagtt
      781 gttttcaaag caaatgaact agcgattagt cgctatgact taacggagca tgaaaccaag
      841 ctaattttat gctgtgtggc actactcaac cccacgattg aaaaccctac aaggaaagaa
      901 cggacggtat cgttcactta taaccaatac gttcagatga tgaacatcag tagggaaaat
      961 gcttatggtg tattagctaa agcaaccaga gagctgatga cgagaactgt ggaaatcagg
     1021 aatcctttgg ttaaaggctt tgagattttc cagtggacaa actatgccaa gttctcaagc
     1081 gaaaaattag aattagtttt tagtgaagag atattgcctt atcttttcca gttaaaaaaa
     1141 ttcataaaat ataatctgga acatgttaag tcttttgaaa acaaatactc tatgaggatt
     1201 tatgagtggt tattaaaaga actaacacag aagaaaactc acaaggcaaa tatagagatt
     1261 agccttgatg aatttaagtt catgttaatg cttgaaaata actaccatga gtttaaaagg
     1321 cttaaccaat gggttttgaa accaataagt aaagatttaa acacttacag caatatggaa
     1381 ttggtggttg ataagcgagg ccgcccgact gatacgttga ttttccaagt tgaactagat
     1441 agacaaatgg atctcgtaac cgaacttgag aacaaccaga taaaaatgaa tggtgacaaa
     1501 ataccaacaa ccattacatc agattcctac ctacataacg gactaagaaa aacactacac
     1561 gatgctttaa ctgcaaaaat ccagctcacc agttttgagg caaaattttt gagtgacatg
     1621 caaagtaagt atgatctcaa tggttcgttc tcatggctca cgcaaaaaca acgaaccaca
     1681 ctagagaaca tactggctaa atacggaagg atctgatctc gtacgaagca tgctaatttt
     1741 gtgttactca tgcatgtgtg aattcacacc tcgagtaagg atccgcccct ctccctcccc
     1801 cccccctaac gttactggcc gaagccgctt ggaataaggc cggtgtgcgt ttgtctatat
     1861 gttattttcc accatattgc cgtcttttgg caatgtgagg gcccggaaac ctggccctgt
     1921 cttcttgacg agcattccta ggggtctttc ccctctcgcc aaaggaatgc aaggtctgtt
     1981 gaatgtcgtg aaggaagcag ttcctctgga agcttcttga agacaaacaa cgtctgtagc
     2041 gaccctttgc aggcagcgga accccccacc tggcgacagg tgcctctgcg gccaaaagcc
     2101 acgtgtataa gatacacctg caaaggcggc acaaccccag tgccacgttg tgagttggat
     2161 agttgtggaa agagtcaaat ggctctcctc aagcgtattc aacaaggggc tgaaggatgc
     2221 ccagaaggta ccccattgta tgggatctga tctggggcct cggtgcacat gctttacatg
     2281 tgtttagtcg aggttaaaaa aacgtctagg ccccccgaac cacggggacg tggttttcct
     2341 ttgaaaaaca cgatgataat atggccacaa ccatggtgag caagggcgag gagctgttca
     2401 ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg
     2461 tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca
     2521 ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc
     2581 agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc
     2641 ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc
     2701 gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg
     2761 acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca
     2821 acgtctatat catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc
     2881 acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg
     2941 gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca
     3001 aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga
     3061 tcactctcgg catggacgag ctgtacaagt aaagcggccg cgactctaga tcataatcag
     3121 ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa
     3181 cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg
     3241 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     3301 tagttgtggt ttgtccaaac tcatcaatgt atcttaaatc gaattggtca ggtggcactt
     3361 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
     3421 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
     3481 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
     3541 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
     3601 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
     3661 aagaacgttc tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
     3721 gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
     3781 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
     3841 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
     3901 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
     3961 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
     4021 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
     4081 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
     4141 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
     4201 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
     4261 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
     4321 cactgattaa gcattggtaa ctgtcagacc aagtttaatt aactttaaat aatgccaatt
     4381 atttaaagtt accaactatt ttgtccgccc acaggtaccc tgtgaatgcg caaaccaacc
     4441 cttggcagaa catatccatc gcgtccgcca tctccagcag ccgcacgcgg cgcatctcgg
     4501 gcagcgttgg gtcctggcca cgggtgcgca tgatcgtgct cctgtcgttg aggacccggc
     4561 taggctggcg gggttgcctt actggttagc agaatgaatc accgatacgc gagcgaacgt
     4621 gaagcgactg ctgctgcaaa acgtctgcga cctgagcaac aacatgaatg gtcttcggtt
     4681 tccgtgtttc gtaaagtctg gaaacgcgga agtcagcgcc ctgcaccatt atgttccgga
     4741 ac
//
