LOCUS       62056_12000        6303 bp DNA     circular SYN 26-MAY-2020
DEFINITION  Cloning vector pH-TEVp, complete sequence.
ACCESSION   MN996837
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6303)
  AUTHORS   Han X, Yang J, Zeng F, Weng J, Zhang Y, Peng Q, Shen L, Ding S, Liu 
            K, Gao Y.
  TITLE     Programmable Synthetic Protein Circuits for the Identification and 
            Suppression of Hepatocellular Carcinoma
  JOURNAL   Mol Ther Oncolytics 17, 70-82 (2020)
  PUBMED    32322664
REFERENCE   2  (bases 1 to 6303)
  AUTHORS   Liu K, Yang J, Ding S.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2020) Second Department of Hepatobiliary Surgery, 
            Zhujiang Hospital, No. 253, Industrial Avenue, Haizhu District, 
            Guangzhou, Guangdong 510245, China
REFERENCE   3  (bases 1 to 6303)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Ther 
            Oncolytics 17, 70-82 (2020)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (27-JAN-2020) Second Department of Hepatobiliary Surgery, Zhujiang 
            Hospital, No. 253, Industrial Avenue, Haizhu District, Guangzhou, 
            Guangdong 510245, China"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6303
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             866..1612
                     /codon_start=1
                     /transl_table=11
                     /product="TEVp"
                     /label=TEVp
                     /protein_id="QJX60180.1"
                     /translation="MGRSGESLFKGPRDYNPISSTICHLTNESDGHTTSLYGIGFGPFI
                     ITNKHLFRRNNGTLLVQSLHGVFKVKNTTTLQQHLIDGRDMIIIRMPKDFPPFPQKLKF
                     REPQREERICLVTTNFQTGGSGSKSMSSMVSDTSCTFPSSDGIFWKHWIQTKDGQCGSP
                     LVSTRDGFIVGIHSASNFTNTNNYFTSVPKNFMELLTNQEAQQWVSGWRLNADSVLWGG
                     HKVFMVKPEEPFQPVKEATQLMNGSA"
     polyA_signal    1660..1884
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      1930..2358
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        2372..2701
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             2768..3802
                     /label=HygR
                     /note="hygromycin B phosphotransferase"
     polyA_signal    3979..4112
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4149..4165)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(4173..4189)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4197..4227)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(4242..4263)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(4551..5136)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5310..6167)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(6168..6272)
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc
      841 gctaggaatt cgcggccgcg ccaccatggg cagatctggg gaatcgctgt ttaaagggcc
      901 acgggattac aatcctattt catccactat atgccatctg accaatgaat cagatggcca
      961 tacaacttcc ctatacggaa tcggatttgg accatttatc atcacgaata agcatctgtt
     1021 tcgccggaat aacggaactc tcctggtgca gagcctccat ggtgtgttta aggttaaaaa
     1081 cacaacaaca ttgcagcaac acctcattga cggccgggat atgatcataa tccgtatgcc
     1141 caaagatttc cctccatttc ctcagaagct caaatttcgt gagccacaga gggaagaacg
     1201 gatatgtctg gtcaccacca atttccagac cggaggcagt ggatctaaat ccatgagcag
     1261 catggtgagc gatacctctt gtacctttcc cagttctgat ggtatattct ggaaacactg
     1321 gattcagaca aaggacggac aatgcggatc tcctcttgtt tcgacgaggg atgggttcat
     1381 cgttggcatt cactccgcat ccaacttcac caacacaaac aactacttca cgtccgtgcc
     1441 taagaatttc atggaacttc ttacgaatca agaagcccag cagtgggttt ctggatggcg
     1501 cctcaacgcc gacagtgtgt tgtggggcgg acacaaagtg tttatggtga aaccagagga
     1561 accgttccag ccagtgaagg aggccacaca attaatgaac ggatccgcgt aaccgctcga
     1621 gaattgttgt tgttgtttaa acccgctgat cagcctcgac tgtgccttct agttgccagc
     1681 catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg
     1741 tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc
     1801 tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg
     1861 ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctctaggg
     1921 ggtatcccca cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
     1981 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct
     2041 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt
     2101 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac
     2161 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct
     2221 ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt
     2281 ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac
     2341 aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc
     2401 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg
     2461 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     2521 agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
     2581 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
     2641 tgcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa
     2701 aaagctcccg ggagcttgta tatccatttt cggatctgat caagagacag gatgaggatc
     2761 gtttcgcatg aaaaagcctg aactcaccgc gacgtctgtc gagaagtttc tgatcgaaaa
     2821 gttcgacagc gtctccgacc tgatgcagct ctcggagggc gaagaatctc gtgctttcag
     2881 cttcgatgta ggagggcgtg gatatgtcct gcgggtaaat agctgcgccg atggtttcta
     2941 caaagatcgt tatgtttatc ggcactttgc atcggccgcg ctcccgattc cggaagtgct
     3001 tgacattggg gaattcagcg agagcctgac ctattgcatc tcccgccgtg cacagggtgt
     3061 cacgttgcaa gacctgcctg aaaccgaact gcccgctgtt ctgcagccgg tcgcggaggc
     3121 catggatgcg atcgctgcgg ccgatcttag ccagacgagc gggttcggcc cattcggacc
     3181 gcaaggaatc ggtcaataca ctacatggcg tgatttcata tgcgcgattg ctgatcccca
     3241 tgtgtatcac tggcaaactg tgatggacga caccgtcagt gcgtccgtcg cgcaggctct
     3301 cgatgagctg atgctttggg ccgaggactg ccccgaagtc cggcacctcg tgcacgcgga
     3361 tttcggctcc aacaatgtcc tgacggacaa tggccgcata acagcggtca ttgactggag
     3421 cgaggcgatg ttcggggatt cccaatacga ggtcgccaac atcttcttct ggaggccgtg
     3481 gttggcttgt atggagcagc agacgcgcta cttcgagcgg aggcatccgg agcttgcagg
     3541 atcgccgcgg ctccgggcgt atatgctccg cattggtctt gaccaactct atcagagctt
     3601 ggttgacggc aatttcgatg atgcagcttg ggcgcagggt cgatgcgacg caatcgtccg
     3661 atccggagcc gggactgtcg ggcgtacaca aatcgcccgc agaagcgcgg ccgtctggac
     3721 cgatggctgt gtagaagtac tcgccgatag tggaaaccga cgccccagca ctcgtccgga
     3781 tcgggagatg ggggaggcta actgagcggg actctggggt tcgaaatgac cgaccaagcg
     3841 acgcccaacc tgccatcacg agatttcgat tccaccgccg ccttctatga aaggttgggc
     3901 ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg
     3961 gagttcttcg cccaccccaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     4021 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     4081 aaactcatca atgtatctta tcatgtctgt ataccgtcga cctctagcta gagcttggcg
     4141 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
     4201 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
     4261 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
     4321 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
     4381 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     4441 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     4501 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     4561 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     4621 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     4681 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     4741 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     4801 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     4861 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     4921 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     4981 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     5041 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggttt ttttgtttgc
     5101 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     5161 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     5221 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     5281 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     5341 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     5401 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     5461 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     5521 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     5581 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     5641 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     5701 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     5761 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     5821 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     5881 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     5941 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     6001 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     6061 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     6121 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     6181 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     6241 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
     6301 gtc
//
