LOCUS       V013834                 5654 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V013834
VERSION     V013834
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5654)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5654
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1372..1476
                     /label="AmpR promoter"
     CDS             1477..2289
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
     rep_origin      complement(2456..3000)
                     /direction=LEFT
                     /label="p15A ori"
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells
                     that contain a second plasmid with the ColE1 origin."
     CDS             complement(3279..4922)
                     /gene="groEL"
                     /label="Chaperonin GroEL"
                     /note="Chaperonin GroEL from Escherichia coli O8 (strain
                     IAI1). Accession#: B7M8Q4"
     CDS             complement(5161..5214)
                     /label="S loop"
                     /note="GroES chaperone mobile loop that interacts with
                     GroEL"
ORIGIN
        1 atggctgaaa cgcaaaatga tccgctattg ccgggatatt catttaatgc ccatctggtc
       61 gccgggctga cgccaattga agcgaatgga tatctggatt tttttatcga tcgtccgttg
      121 ggcatgaagg gatatattct taacctgacc atccgcggag agggcgtcat taataataat
      181 ggcgagcagt ttgtctgtcg gcctggcgat atattattgt ttccgccggg cgagattcat
      241 cactatggac ggcatccgga cgccagcgag tggtatcacc agtgggttta tttccggcct
      301 cgcgcctact ggcaggagtg gctgacctgg ccgacaatct ttgcccagac aggatttttc
      361 cgcccggacg aggcgcgcca gccgcatttc agcgaactgt tcgggcagat catcagcgcc
      421 gggcaagggg aaggtcgcta ttctgagcta ctggcgatca atctgctgga gcagttgttg
      481 ctcagacgta tggcggtaat taatgagtcg ttgcacccgc cgatggatag ccgtgtgcgc
      541 gatgcctgcc agtatatcag cgaccatctg gcggacagcc attttgatat cgccagcgtc
      601 gcccagcatg tctgcctgtc gccctcccgg ttatcacatc tgttccgcca gcagttaggc
      661 attagcgtat tgagctggcg cgaagatcag cgtatcagcc aggcgaaact tctgcttagc
      721 actacgcgaa tgccgatagc gaccgttggg cgcaatgttg gatttgacga tcagctctat
      781 ttttcgcggg tatttaaaaa atgcaccggg gcaagtccta gcgagttcag ggccggatgt
      841 gaataaaacg gctttgccgc ggccctctca cttccctgtt aagtatcttc ctggcatctt
      901 ccaggaaatc tccgccccgt tcgtaagcca tttccgctcg ccgcagtcga acgaccgagc
      961 gtagcgagtc agtgagcgag gaagcggaat atatcctgta tcacatattc tgctgacgca
     1021 ccggtgcagc cttttttctc ctgccacatg aagcacttca ctgacaccct catcagtgcc
     1081 aacatagtaa gccagtatac actccgctag cgctgatgtc cggcggtgct tttgccgtta
     1141 cgcaccaccc cgtcagtagc tgaacaggag ggacagctga tagaaacaga agccactgga
     1201 gcacctcaaa aacaccatca tacactaaat cagtaagttg gcagcatcac ccgacgcact
     1261 ttgcgccgaa taaatacctg tgacggaaga tcacttcgca gaataaataa atcctggtgt
     1321 ccctgttgat accgggaagc cctgggccaa cttttggcga aaatgagacg tcgcggaacc
     1381 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     1441 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gccatattca acgggaaacg
     1501 tcttgctcta ggccgcgatt aaattccaac atggatgctg atttatatgg gtataaatgg
     1561 gctcgcgata atgtcgggca atcaggtgcg acaatctatc gattgtatgg gaagcccgat
     1621 gcgccagagt tgtttctgaa acatggcaaa ggtagcgttg ccaatgatgt tacagatgag
     1681 atggtcagac taaactggct gacggaattt atgcctcttc cgaccatcaa gcattttatc
     1741 cgtactcctg atgatgcatg gttactcacc actgcgatcc ccgggaaaac agcattccag
     1801 gtattagaag aatatcctga ttcaggtgaa aatattgttg atgcgctggc agtgttcctg
     1861 cgccggttgc attcgattcc tgtttgtaat tgtcctttta acagcgatcg cgtatttcgt
     1921 ctcgctcagg cgcaatcacg aatgaataac ggtttggttg atgcgagtga ttttgatgac
     1981 gagcgtaatg gctggcctgt tgaacaagtc tggaaagaaa tgcataaact tttgccattc
     2041 tcaccggatt cagtcgtcac tcatggtgat ttctcacttg ataaccttat ttttgacgag
     2101 gggaaattaa taggttgtat tgatgttgga cgagtcggaa tcgcagaccg ataccaggat
     2161 cttgccatcc tatggaactg cctcggtgag ttttctcctt cattacagaa acggcttttt
     2221 caaaaatatg gtattgataa tcctgatatg aataaattgc agtttcattt gatgctcgat
     2281 gagtttttct aaactgcgat gagtggcagg gcggggcgta atttttttaa ggcagttatt
     2341 ggtgccctta aacgcctggt gctacgcctg aataagtgat aataagcgga tgaatggcag
     2401 aaattcgaag aatagttacg gcttatgaca tctttgtgga cacatcattc acttttttcc
     2461 ataggctccg cccccctgac aagcatcacg aaatctgacg ctcaaatcag tggtggcgaa
     2521 acccgacagg actataaaga taccaggcgt ttcccctggc ggctccctcg tgcgctctcc
     2581 tgttcctgcc tttcggttta ccggtgtcat tccgctgtta tggccgcgtt tgtctcattc
     2641 cacgcctgac actcagttcc gggtaggcag ttcgctccaa gctggactgt atgcacgaac
     2701 cccccgttca gtccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     2761 aaagacatgc aaaagcacca ctggcagcag ccactggtaa ttgatttaga ggagttagtc
     2821 ttgaagtcat gcgccggtta aggctaaact gaaaggacaa gttttggtga ctgcgctcct
     2881 ccaagccagt tacctcggtt caaagagttg gtagctcaga gaaccttcga aaaaccgccc
     2941 tgcaaggcgg ttttttcgtt ttcagagcaa gagattacgc gcagaccaaa acgatctcaa
     3001 gaagatcatc ttattaatca gataaatatt tctagatttc agtgcaattt atctcttcaa
     3061 atgtagcacc tgaagtcagc cccatacgat ataagttgta attctcatgt ttgacagctt
     3121 atcatcgata agcttggtac cagatctgat atcggcgcct cgaggcgcca ctcgcgtcgt
     3181 ccgtgtctga atcttatact agaaagatga ccaaaagaaa aacccccaga catttctgcc
     3241 cgggggtttg tttatttctg cgaggtgcag ggcaattaca tcatgccgcc catgccaccc
     3301 atgccgccca taccgccagc agcgcctaag tcagctgcat cgtttttcgg caggtcggta
     3361 accatgcatt cggtggtgat catcaggcca gccacagaag ctgcgtactg cagagcagaa
     3421 cgagttactt tggttggatc caggataccc atgtcgatca tgttgccgta ttcttcggtt
     3481 gctgcgttgt aaccgtagtt gccgtcgccg cctttaacgg tgttagcaac aacagacggt
     3541 tcttcgccgc agttcaatac gatctgacgc agcggagctt ccattgcacg cagtgcaact
     3601 ttgataccca cgttctggtc ttcgttctga ccacgcaggt cagccagttt agacgctacg
     3661 cggatcagcg caacaccacc accagcaacc acgccttctt ctaccgcagc acgggtcgcg
     3721 tgcagggcat cttcaacgcg tgcttttttc tctttcattt caacttcggt agcagcaccc
     3781 actttgataa ctgcaacgcc gcctgccagt ttcgctacgc gttcctgcag tttttcacgg
     3841 tcgtagtcag aagttgcttc ttcaatctgc tgacggatct gagcaacacg gccctggatt
     3901 gcagcttctt cacccacgcc atcgatgata gtggtggtgt ctttgttgat cacaacacgt
     3961 ttagcctgac ccaggtcttc cagggttgct ttttccagct ccataccgat ctcttcagag
     4021 atcacggtac cgccagtcag ggttgcgata tcctgcagca tagctttacg acgatcgccg
     4081 aagcccggtg ctttaaccgc agcgactttc acgatgccac gcatggtgtt aacaaccaga
     4141 gttgccagcg cttcgccttc tacatcttca gcgatgatca gcagcggttt gcctgctttg
     4201 gcaacagctt ccagaaccgg cagcatttcg cggatgttgg agattttctt gtcagccagc
     4261 aggatgaacg ggctttccag ttctactgcg ccagtttccg gcttgttgat gaagtaagga
     4321 gacaggtagc cacggtcgaa ctgcatacct tcaaccacgt ccagttcgtc ctgcagaccg
     4381 gtaccgtctt caacggtgat aacgccttct ttaccgactt tgtccatcgc ttcagcgatc
     4441 agtttaccta cggtttcgtc ggagttagcg gagatggtac caacctgagc aatcgcttta
     4501 gagtcagagc atggtacgga cagcgctttc agttcttcaa ctgcagcggt aaccgctttg
     4561 tcgataccac gtttcaggtc catcgggttc atgcccgcag caacagcttt cagaccttca
     4621 gtgatgatag cctgagccag tacggttgca gtggtggtac cgtcgcctgc agcgtcgttt
     4681 gctttagagg caacttcttt caccatctgc gcacccatat tttcgaactt gtcttccagt
     4741 tcgatttcac gagcaacgga aacaccatct ttggtgatgg tcggtgcacc gaaagattta
     4801 tccagaacta cgttacggcc ttttggaccg agggtaactt tcactgcatc tgccagtacg
     4861 tttacgccgc gcagcatttt cacacgagcg tcgttaccga attttacgtc tttagctgcc
     4921 attatcttta ttccttaaat tcgtatgttc agtgtcgtgc gcggattacg cttcaacaat
     4981 tgccagaatg tcgctttcgg acatgatcaa cacttcttca ttgtcgatct tctcagattt
     5041 cacaccgtag ccatcgttga aaataacgat gtcgccaact ttcacatcca gcggcttcac
     5101 ttcgccattt tcaaggatac ggccattgcc gacagccagc acttcgccgc gggtggattt
     5161 agccgctgca gagccggtca gaacgatgcc gccagcagat ttagtttcaa cttctttacg
     5221 cttgacgatc acgcgatcat gcaatggacg aatattcatt gataactctc ctttgagaaa
     5281 gtccgtatct gttatgggtg acgcctcgag ccatggtctt actccatcca gaaaaacagg
     5341 tatggagaaa ttatagagag tcgcggcaaa aaccgtcagg caggatccgc taatcttatg
     5401 gacaaaaatg ctaatgcttt gcaaagtgtg acgctgtgca aatattcaat gtggacattc
     5461 cagccatagt tatagacact tctgttactt aattttatcg cctgaactgt acgcttttgt
     5521 tacaaagcgc ttttcacaag cggggttgat acgtgctttc atcaagcgca aagtcttgcg
     5581 gagacggaag ctctgtcgtc ctggtcgata tggacaattt gtttcttctc tgaacatcgg
     5641 ggggtagaga aatc
//
