LOCUS       Exported                3338 bp DNA     circular SYN 03-SEP-2024
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3338)
  AUTHORS   caoheibi
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 3338)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 3338)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3338
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    534..556
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA
                     (truncated)"
     promoter        628..973
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     misc_feature    983..1090
                     /label=MCS
                     /note="pBluescript multiple cloning site"
     polyA_signal    1144..1320
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    1330..1354
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      complement(1445..2033)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2207..3019)
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     rep_origin      3310..3338
                     /label=pSa ori
                     /note="origin of replication from bacterial plasmid pSa"
ORIGIN
        1 tttttatccc cggaagcctg tggatagagg gtagttatcc acgtgaaacc gctaatgccc
       61 cgcaaagcct tgattcacgg ggctttccgg cccgctccaa aaactatcca cgtgaaatcg
      121 ctaatcaggg tacgtgaaat cgctaatcgg agtacgtgaa atcgctaata aggtcacgtg
      181 aaatcgctaa tcaaaaaggc acgtgagaac gctaatagcc ctttcagatc aacagcttgc
      241 aaacacccct cgctccggca agtagttaca gcaagtagta tgttcaatta gcttttcaat
      301 tatgaatata tatatcaatt attggtcgcc cttggcttgt ggacaatgcg ctacgcgcac
      361 cggctccgcc cgtggacaac cgcaagcggt tgcccaccgt cgagcgcctt tgcccacaac
      421 ccggcggccg gccgcaacag atcgttttat aaattttttt ttttgaaaaa gaaaaagccc
      481 gaaaggcggc aacctctcgg gcttctggat ttccgatccc cggaattaga tcttggcagg
      541 atatattgtg gtgtaacgtt atcagcttgg tacgtacccc tactccaaaa atgtcaaaga
      601 tacagtctca gaagaccaaa gggctattga gacttttcaa caaagggtaa tttcgggaaa
      661 cctcctcgga ttccattgcc cagctatctg tcacttcatc gaaaggacag tagaaaagga
      721 aggtggctcc tacaaatgcc atcattgcga taaaggaaag gctatcattc aagatgcctc
      781 tgccgacagt ggtcccaaag atggaccccc acccacgagg agcatcgtgg aaaaagaaga
      841 cgttccaacc acgtcttcaa agcaagtgga ttgatgtgac atctccactg acgtaaggga
      901 tgacgcacaa tcccactatc cttcgcaaga cccttcctct atataaggaa gttcatttca
      961 tttggagagg acagcccaag ctgagctcca ccgcggtggc ggccgctcta gaactagtgg
     1021 atcccccggg ctgcaggaat tcgatatcaa gcttatcgat accgtcgacc tcgagggggg
     1081 gcccggtacc aattcggtac gctgaaatca ccagtctctc tctacaaatc tatctctctc
     1141 tattttctcc ataaataatg tgtgagtagt ttcccgataa gggaaattag ggttcttata
     1201 gggtttcgct catgtgttga gcatataaga aacccttagt atgtatttgt atttgtaaaa
     1261 tacttctatc aataaaattt ctaattccta aaaccaaaat ccagtactaa aatccagatc
     1321 cactagcctt gacaggatat attggcgggt aaactaagtc gctgtatgtg tttgtttgag
     1381 atctcatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     1441 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     1501 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     1561 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     1621 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     1681 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     1741 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     1801 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     1861 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
     1921 ttaccttcgg aagaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     1981 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     2041 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     2101 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     2161 ttaaatcaat ctaaagtata tatgtgtaac attggtctag tgattagaaa aactcatcga
     2221 gcatcaaatg aaactgcaat ttattcatat caggattatc aataccatat ttttgaaaaa
     2281 gccgtttctg taatgaagga gaaaactcac cgaggcagtt ccataggatg gcaagatcct
     2341 ggtatcggtc tgcgattccg actcgtccaa catcaataca acctattaat ttcccctcgt
     2401 caaaaataag gttatcaagt gagaaatcac catgagtgac gactgaatcc ggtgagaatg
     2461 gcaaaagttt atgcatttct ttccagactt gttcaacagg ccagccatta cgctcgtcat
     2521 caaaatcact cgcatcaacc aaaccgttat tcattcgtga ttgcgcctga gcgagacgaa
     2581 atacgcgatc gctgttaaaa ggacaattac aaacaggaat cgaatgcaac cggcgcagga
     2641 acactgccag cgcatcaaca atattttcac ctgaatcagg atattcttct aatacctgga
     2701 atgctgtttt ccctgggatc gcagtggtga gtaaccatgc atcatcagga gtacggataa
     2761 aatgcttgat ggtcggaaga ggcataaatt ccgtcagcca gtttagtctg accatctcat
     2821 ctgtaacatc attggcaacg ctacctttgc catgtttcag aaacaactct ggcgcatcgg
     2881 gcttcccata caatcggtag attgtcgcac ctgattgccc gacattatcg cgagcccatt
     2941 tatacccata taaatcagca tccatgttgg aatttaatcg cggccttgag caagacgttt
     3001 cccgttgaat atggctcata acaccccttg tattactgtt tatgtaagca gacagtttta
     3061 ttgttcatga tgatatattt ttatcttgtg caatgtaaca tcagagattt tgagacacaa
     3121 cgtggctttg ttgaataaat cgaacttttg ctgagttgaa ggatcagatc acgcatcttc
     3181 ccgacaacgc agaccgttcc gtggcaaagc aaaagttcaa aatcaccaac tggtccacct
     3241 acaacaaagc tctcatcaac cgtggctccc tcactttctg gctggatgat ggggcgattc
     3301 aggcgatccc catccaacag cccgccgtcg agcgggct
//
