LOCUS       pGreenII_0000_(N        3294 bp DNA     circular SYN 17-NOV-2023
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    pGreenII 0000 (No LUC)
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3294)
  TITLE     Plasmids to assay for nuclease cleavage in planta
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3294)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
FEATURES             Location/Qualifiers
     source          1..3294
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(30..54)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     159..176
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    293..314
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        329..359
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    367..383
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     391..407
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        428..446
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    459..566
                     /label=MCS
                     /note="pBluescript multiple cloning site"
     promoter        complement(575..593)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(603..619)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    complement(784..806)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA
                     (truncated)"
     rep_origin      complement(933..1368)
                     /direction=LEFT
                     /label=pSa ori
                     /note="origin of replication from bacterial plasmid pSa"
     CDS             1659..2471
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     rep_origin      2645..3233
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gatctcaaac aaacacatac agcgacttag tttacccgcc aatatatcct gtcaaggcct
       61 tcatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
      121 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
      181 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
      241 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga
      301 gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt
      361 gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca
      421 agcgcgcaat taaccctcac taaagggaac aaaagctgga gctccaccgc ggtggcggcc
      481 gctctagaac tagtggatcc cccgggctgc aggaattcga tatcaagctt atcgataccg
      541 tcgacctcga gggggggccc ggtacccaat tcgccctata gtgagtcgta ttacgcgcgc
      601 tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat
      661 cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat
      721 cgcccttccc aacagttgcg cagcctgaat ggcgaatgga aattgtaagc gttaatgtta
      781 acgttacacc acaatatatc ctgccaagat ctaattccgg ggatcggaaa tccagaagcc
      841 cgagaggttg ccgcctttcg ggctttttct ttttcaaaaa aaaaaattta taaaacgatc
      901 tgttgcggcc ggccgccggg ttgtgggcaa aggcgctcga cggtgggcaa ccgcttgcgg
      961 ttgtccacgg gcggagccgg tgcgcgtagc gcattgtcca caagccaagg gcgaccaata
     1021 attgatatat atattcataa ttgaaaagct aattgaacat actacttgct gtaactactt
     1081 gccggagcga ggggtgtttg caagctgttg atctgaaagg gctattagcg ttctcacgtg
     1141 cctttttgat tagcgatttc acgtgacctt attagcgatt tcacgtactc cgattagcga
     1201 tttcacgtac cctgattagc gatttcacgt ggatagtttt tggagcgggc cggaaagccc
     1261 cgtgaatcaa ggctttgcgg ggcattagcg gtttcacgtg gataactacc ctctatccac
     1321 aggcttccgg ggataaaaaa gcccgctcga cggcgggctg ttggatgggg atcgcctgaa
     1381 tcgccccatc atccagccag aaagtgaggg agccacggtt gatgagagct ttgttgtagg
     1441 tggaccagtt ggtgattttg aacttttgct ttgccacgga acggtctgcg ttgtcgggaa
     1501 gatgcgtgat ctgatccttc aactcagcaa aagttcgatt tattcaacaa agccacgttg
     1561 tgtctcaaaa tctctgatgt tacattgcac aagataaaaa tatatcatca tgaacaataa
     1621 aactgtctgc ttacataaac agtaatacaa ggggtgttat gagccatatt caacgggaaa
     1681 cgtcttgctc aaggccgcga ttaaattcca acatggatgc tgatttatat gggtataaat
     1741 gggctcgcga taatgtcggg caatcaggtg cgacaatcta ccgattgtat gggaagcccg
     1801 atgcgccaga gttgtttctg aaacatggca aaggtagcgt tgccaatgat gttacagatg
     1861 agatggtcag actaaactgg ctgacggaat ttatgcctct tccgaccatc aagcatttta
     1921 tccgtactcc tgatgatgca tggttactca ccactgcgat cccagggaaa acagcattcc
     1981 aggtattaga agaatatcct gattcaggtg aaaatattgt tgatgcgctg gcagtgttcc
     2041 tgcgccggtt gcattcgatt cctgtttgta attgtccttt taacagcgat cgcgtatttc
     2101 gtctcgctca ggcgcaatca cgaatgaata acggtttggt tgatgcgagt gattttgatg
     2161 acgagcgtaa tggctggcct gttgaacaag tctggaaaga aatgcataaa cttttgccat
     2221 tctcaccgga ttcagtcgtc actcatggtg atttctcact tgataacctt atttttgacg
     2281 aggggaaatt aataggttgt attgatgttg gacgagtcgg aatcgcagac cgataccagg
     2341 atcttgccat cctatggaac tgcctcggtg agttttctcc ttcattacag aaacggcttt
     2401 ttcaaaaata tggtattgat aatcctgata tgaataaatt gcagtttcat ttgatgctcg
     2461 atgagttttt ctaatcacta gaccaatgtt acacatatat actttagatt gatttaaaac
     2521 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     2581 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     2641 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     2701 taccagcggt ggtttgtttg ccggatcaag agctaccaac tcttcttccg aaggtaactg
     2761 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     2821 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     2881 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     2941 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     3001 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     3061 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     3121 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     3181 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     3241 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atga
//
