LOCUS       40924_22627       12920 bp DNA     circular SYN 13-MAY-2021
DEFINITION  R2D2: Expresses a fusion of auxin-sensitive DII to nuclear 3xVenus 
            and auxin-insensitive mDII to nuclear tdTomato, both from the 
            Arabidopsis RPS5A promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12920)
  AUTHORS   Liao CY, Smet W, Brunoud G, Yoshida S, Vernoux T, Weijers D
  TITLE     Reporters for sensitive and quantitative measurement of auxin 
            response.
  JOURNAL   Nat Methods. 2015 Mar;12(3):207-10. doi: 10.1038/nmeth.3279. Epub 
            2015 Feb 2.
  PUBMED    25643149
REFERENCE   2  (bases 1 to 12920)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 12920)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.3279"; journalName: "Nat Methods"; date: "2015-03"; 
            volume: "12"; issue: "3"; pages: "207-10"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..12920
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(1721..1739)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1752..1776
                     /label=attB1
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1912..1932
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1933..3360
                     /codon_start=1
                     /label=tdTomato
                     /note="tandem dimeric (pseudo-monomeric) derivative of
                     DsRed (Shaner et al., 2004)"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     promoter        complement(5352..5370)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    5383..5407
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5543..5563
                     /codon_start=1
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /translation="PKKKRKV"
     CDS             5594..6310
                     /codon_start=1
                     /label=Venus
                     /note="yellow fluorescent protein (YFP) with fast and
                     efficient maturation (Nagai et al., 2002)"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             6329..6349
                     /codon_start=1
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /translation="PKKKRKV"
     CDS             6380..7096
                     /codon_start=1
                     /product="yellow fluorescent protein (YFP) with fast and
                     efficient maturation (Nagai et al., 2002)"
                     /label=Venus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             7130..7849
                     /codon_start=1
                     /product="yellow fluorescent protein (YFP) with fast and
                     efficient maturation (Nagai et al., 2002)"
                     /label=Venus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     promoter        complement(8133..8151)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(8172..8188)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8172..8188)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8185..8207)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8196..8212
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(8220..8250)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(8265..8286)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     primer_bind     complement(8403..8420)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    8525..8549
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      complement(8640..9228)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(9402..10214)
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     rep_origin      10505..10940
                     /label=pSa ori
                     /note="origin of replication from bacterial plasmid pSa"
     misc_feature    11067..11089
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA
                     (truncated)"
     polyA_signal    11097..11273
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(11672..12232)
                     /codon_start=1
                     /label=DHFR
                     /note="mouse dihydrofolate reductase"
                     /translation="MVRPLNCIVAVSQNMGIGKNGDRPWPPLRNEFKYFQRMTTTSSVE
                     GKQNLVIMGRKTWFSIPEKNRPLKDRINIVLSRELKEPPRGAHFLAKSLDDALRLIEQP
                     ELASKVDMVWIVGGSSVYQEAMNQPGHLRLFVTRIMQEFESDTFFPEIDLGKYKLLPEY
                     PGVLSEVQEEKGIKYKFEVYEKKD"
     promoter        complement(12265..12610)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     12828..12844
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        12854..12872
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 tagttggaat aggatttccc gggccataat cgtgagtaga tatattactc aacttttgat
       61 tcgctatttg cagtgcacct gtggcgttca tcacatcttt tgtggcactg tttgcactgg
      121 tcattgctat tacaaaggac cttcctgatg ttgaaggaga tcgaaagtaa gtaactgcac
      181 gcataaccat tttctttccg ctctttggct caatccattt gacagtcaaa gacaatgttt
      241 aaccagctcc gtttgatata ttgtctttat gtgtttgttc aagcatgttt agttaatcat
      301 gcctttgatt gatcttgaat aggttccaaa tatcaaccct ggcaacaaaa cttggagtga
      361 gaaacattgc attcctcggt tctggacttc tgctagtaaa ttatgcttca gccatatcac
      421 tagctttcta catgcctcag gtgaattcat ctatttccgt cttaactatt tcggttaatt
      481 aaagcacgaa caccattact gcatgtagaa gcttgataaa ctatcgccac caatttattt
      541 ttgttgcgat attgttactt tcctcagtat gcagctttga aaagaccaac cctcttatcc
      601 tttaacaatg aacaggtttt tagaggtagc ttgatgattc ctgcacatgt gatcttggct
      661 tcaggcttaa ttttccaggt aaagcattat gagatactct tatatctctt acatactttt
      721 gagataatgc acaagaactt cataactata tgctttagtt tctgcatttg acactgccaa
      781 attcattaat ctctaatatc tttgttgttg atctttggta gacatgggta ctagaaaaag
      841 caaactacac caaggtaaaa tacttttgta caaacataaa ctcgttatca cggaacatca
      901 atggagtgta tatctaacgg agtgtagaaa catttgatta ttgcaggaag ctatctcagg
      961 atattatcgg tttatatgga atctcttcta cgcagagtat ctgttattcc ccttcctcta
     1021 gctttcaatt tcatggtgag gatatgcagt tttctttgta tatcattctt cttcttcttt
     1081 gtagcttgga gtcaaaatcg gttccttcat gtacatacat caaggatatg tccttctgaa
     1141 tttttatatc ttgcaataaa aatgcttgta ccaattgaaa caccagcttt ttgagttcta
     1201 tgatcactga cttggttcta accaaaaaaa aaaatgttta atttacatat ctaaaagtag
     1261 gtttagggaa acctaaacag taaaatattt gtatattatt cgaatttcac tcatcataaa
     1321 aacttaaatt gcaccataaa attttgtttt actattaatg atgtaatttg tgtaacttaa
     1381 gataaaaata atattccgta agttaaccgg ctaaaaccac gtataaacca gggaacctgt
     1441 taaaccggtt ctttactgga taaagaaatg aaagcccatg tagacagctc cattagagcc
     1501 caaaccctaa atttctcatc tatataaaag gagtgacatt agggtttttg ttcgtcctct
     1561 taaagcttct cgttttctct gccgtctctc tcattcgcgc gacgcaaacg atcttcaggt
     1621 gatcttcttt ctccaaatcc tctctcataa ctctgatttc gtacttgtgt atttgagctc
     1681 acgctctgtt tctctcacca cagccgggta cccaattcgc cctatagtga gtcgtattac
     1741 aattcactgg cccaagtttg tacaaaaaag caggctatga aacaaaaaag ctcgaccaaa
     1801 gaaacatcat tcctttccaa taacagggtt gaggtagctc cagtggtggg atggctgccg
     1861 gtgagatcat cccggagagg gaaatccaac tcaatactga agcttatggc tccaaagaag
     1921 aagagaaagg tcatggtgag caagggcgag gaggtcatca aagagttcat gcgcttcaag
     1981 gtgcgcatgg agggctccat gaacggccac gagttcgaga tcgagggcga gggcgagggc
     2041 cgcccctacg agggcaccca gaccgccaag ctgaaggtga ccaagggcgg ccccctgccc
     2101 ttcgcctggg acatcctgtc cccccagttc atgtacggct ccaaggcgta cgtgaagcac
     2161 cccgccgaca tccccgatta caagaagctg tccttccccg agggcttcaa gtgggagcgc
     2221 gtgatgaact tcgaggacgg cggtctggtg accgtgaccc aggactcctc cctgcaggac
     2281 ggcacgctga tctacaaggt gaagatgcgc ggcaccaact tcccccccga cggccccgta
     2341 atgcagaaga agaccatggg ctgggaggcc tccaccgagc gcctgtaccc ccgcgacggc
     2401 gtgctgaagg gcgagatcca ccaggccctg aagctgaagg acggcggcca ctacctggtg
     2461 gagttcaaga ccatctacat ggccaagaag cccgtgcaac tgcccggcta ctactacgtg
     2521 gacaccaagc tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgag
     2581 cgctccgagg gccgccacca cctgttcctg gggcatggca ccggcagcac cggcagcggc
     2641 agctccggca ccgcctcctc cgaggacaac aacatggccg tcatcaaaga gttcatgcgc
     2701 ttcaaggtgc gcatggaggg ctccatgaac ggccacgagt tcgagatcga gggcgagggc
     2761 gagggccgcc cctacgaggg cacccagacc gccaagctga aggtgaccaa gggcggcccc
     2821 ctgcccttcg cctgggacat cctgtccccc cagttcatgt acggctccaa ggcgtacgtg
     2881 aagcaccccg ccgacatccc cgattacaag aagctgtcct tccccgaggg cttcaagtgg
     2941 gagcgcgtga tgaacttcga ggacggcggt ctggtgaccg tgacccagga ctcctccctg
     3001 caggacggca cgctgatcta caaggtgaag atgcgcggca ccaacttccc ccccgacggc
     3061 cccgtaatgc agaagaagac catgggctgg gaggcctcca ccgagcgcct gtacccccgc
     3121 gacggcgtgc tgaagggcga gatccaccag gccctgaagc tgaaggacgg cggccactac
     3181 ctggtggagt tcaagaccat ctacatggcc aagaagcccg tgcaactgcc cggctactac
     3241 tacgtggaca ccaagctgga catcacctcc cacaacgagg actacaccat cgtggaacag
     3301 tacgagcgct ccgagggccg ccaccacctg ttcctgtacg gcatggacga gctgtacaag
     3361 tagctcgaga gatccgatcc ccgatcgttc aaacatttgg caataaagtt tcttaagatt
     3421 gaatcctgtt gccggtcttg cgatgattat catataattt ctgttgaatt acgttaagca
     3481 tgtaataatt aacatgtaat gcatgacgtt atttatgaga tgggttttta tgattagagt
     3541 cccgcaatta tacatttaat acgcgataga aaacaaaata tagcgcgcaa actaggataa
     3601 attatcgcgc gcggtgtcat ctatgtgaat tctagttgga atagggttcg ggccataatc
     3661 gtgagtagat atattactca acttttgatt cgctatttgc agtgcacctg tggcgttcat
     3721 cacatctttt gtggcactgt ttgcactggt cattgctatt acaaaggacc ttcctgatgt
     3781 tgaaggagat cgaaagtaag taactgcacg cataaccatt ttctttccgc tctttggctc
     3841 aatccatttg acagtcaaag acaatgttta accagctccg tttgatatat tgtctttatg
     3901 tgtttgttca agcatgttta gttaatcatg cctttgattg atcttgaata ggttccaaat
     3961 atcaaccctg gcaacaaaac ttggagtgag aaacattgca ttcctcggtt ctggacttct
     4021 gctagtaaat tatgcttcag ccatatcact agctttctac atgcctcagg tgaattcatc
     4081 tatttccgtc ttaactattt cggttaatta aagcacgaac accattactg catgtagaag
     4141 cttgataaac tatcgccacc aatttatttt tgttgcgata ttgttacttt cctcagtatg
     4201 cagctttgaa aagaccaacc ctcttatcct ttaacaatga acaggttttt agaggtagct
     4261 tgatgattcc tgcacatgtg atcttggctt caggcttaat tttccaggta aagcattatg
     4321 agatactctt atatctctta catacttttg agataatgca caagaacttc ataactatat
     4381 gctttagttt ctgcatttga cactgccaaa ttcattaatc tctaatatct ttgttgttga
     4441 tctttggtag acatgggtac tagaaaaagc aaactacacc aaggtaaaat acttttgtac
     4501 aaacataaac tcgttatcac ggaacatcaa tggagtgtat atctaacgga gtgtagaaac
     4561 atttgattat tgcaggaagc tatctcagga tattatcggt ttatatggaa tctcttctac
     4621 gcagagtatc tgttattccc cttcctctag ctttcaattt catggtgagg atatgcagtt
     4681 ttctttgtat atcattcttc ttcttctttg tagcttggag tcaaaatcgg ttccttcatg
     4741 tacatacatc aaggatatgt ccttctgaat ttttatatct tgcaataaaa atgcttgtac
     4801 caattgaaac accagctttt tgagttctat gatcactgac ttggttctaa ccaaaaaaaa
     4861 aaaaatgttt aatttacata tctaaaagta ggtttaggga aacctaaaca gtaaaatatt
     4921 tgtatattat tcgaatttca ctcatcataa aaacttaaat tgcaccataa aattttgttt
     4981 tactattaat gatgtaattt gtgtaactta agataaaaat aatattccgt aagttaaccg
     5041 gctaaaacca cgtataaacc agggaacctg ttaaaccggt tctttactgg ataaagaaat
     5101 gaaagcccat gtagacagct ccattagagc ccaaacccta aatttctcat ctatataaaa
     5161 ggagtgacat tagggttttt gttcgtcctc ttaaagcttc tcgttttctc tgccgtctct
     5221 ctcattcgcg cgacgcaaac gatcttcagg tgatcttctt tctccaaatc ctctctcata
     5281 actctgattt cgtacttgtg tatttgagct cacgctctgt ttctctcacc acagccgggt
     5341 acccaattcg ccctatagtg agtcgtatta caattcactg gcccaagttt gtacaaaaaa
     5401 gcaggctatg aaacaaaaaa gctcgaccaa agaaacatca ttcctttcca ataacagggt
     5461 tgaggtagct ccagtggtgg gatggccgcc ggtgagatca tcccggagag aacccaactc
     5521 catactggat ccaacaatgg ctcccaagaa gaagagaaag gtagatcccc gggctgcagg
     5581 aattaattcg atcatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct
     5641 ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg
     5701 cgatgccacc tacggcaagc tgaccctgaa gctgatctgc accaccggca agctgcccgt
     5761 gccctggccc accctcgtga ccaccctggg ctacggcctg cagtgcttcg cccgctaccc
     5821 cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga
     5881 gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga
     5941 gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa
     6001 catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata tcaccgccga
     6061 caagcagaag aacggcatca aggccaactt caagatccgc cacaacatcg aggacggcgg
     6121 cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct
     6181 gcccgacaac cactacctga gctaccagtc cgccctgagc aaagacccca acgagaagcg
     6241 cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga
     6301 gctgtacaag ttagatccaa caatggctcc caagaagaag agaaaggtag atccccgggc
     6361 tgcaggaatt aattcgatca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc
     6421 catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg
     6481 cgagggcgat gccacctacg gcaagctgac cctgaagctg atctgcacca ccggcaagct
     6541 gcccgtgccc tggcccaccc tcgtgaccac cctgggctac ggcctgcagt gcttcgcccg
     6601 ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt
     6661 ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa
     6721 gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga
     6781 cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg tctatatcac
     6841 cgccgacaag cagaagaacg gcatcaaggc caacttcaag atccgccaca acatcgagga
     6901 cggcggcgtg cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt
     6961 gctgctgccc gacaaccact acctgagcta ccagtccgcc ctgagcaaag accccaacga
     7021 gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat
     7081 ggacgagctg tacaagttag atccaggagc aggagcagga gcaggagcca tggtgagcaa
     7141 gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa
     7201 cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac
     7261 cctgaagctg atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac
     7321 cctgggctac ggcctgcagt gcttcgcccg ctaccccgac cacatgaagc agcacgactt
     7381 cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga
     7441 cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat
     7501 cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta
     7561 caactacaac agccacaacg tctatatcac cgccgacaag cagaagaacg gcatcaaggc
     7621 caacttcaag atccgccaca acatcgagga cggcggcgtg cagctcgctg accactacca
     7681 gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcta
     7741 ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt
     7801 cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagtaag atcctctaga
     7861 gtccgcaaaa atcaccagtc tctctctaca aatctatctc tctctatttt tctccagaat
     7921 aatgtgtgag tagttcccag ataagggaat tagggttctt atagggtttc gctcatgtgt
     7981 tgagcatata agaaaccctt agtatgtatt tgtatttgta aaatacttct atcaataaaa
     8041 tttctaattc ctaaaaccaa aatccagtga cctgcaggca tgcaagctta tctagagcgg
     8101 ccgccaccgc ggtggagctc cagcttttgt tccctttagt gagggttaat tgcgcgcttg
     8161 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
     8221 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
     8281 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
     8341 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
     8401 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     8461 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgaag
     8521 gccttgacag gatatattgg cgggtaaact aagtcgctgt atgtgtttgt ttgagatctc
     8581 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     8641 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     8701 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     8761 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     8821 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     8881 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     8941 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     9001 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     9061 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     9121 ttcggaagaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     9181 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     9241 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     9301 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     9361 tcaatctaaa gtatatatgt gtaacattgg tctagtgatt agaaaaactc atcgagcatc
     9421 aaatgaaact gcaatttatt catatcagga ttatcaatac catatttttg aaaaagccgt
     9481 ttctgtaatg aaggagaaaa ctcaccgagg cagttccata ggatggcaag atcctggtat
     9541 cggtctgcga ttccgactcg tccaacatca atacaaccta ttaatttccc ctcgtcaaaa
     9601 ataaggttat caagtgagaa atcaccatga gtgacgactg aatccggtga gaatggcaaa
     9661 agtttatgca tttctttcca gacttgttca acaggccagc cattacgctc gtcatcaaaa
     9721 tcactcgcat caaccaaacc gttattcatt cgtgattgcg cctgagcgag acgaaatacg
     9781 cgatcgctgt taaaaggaca attacaaaca ggaatcgaat gcaaccggcg caggaacact
     9841 gccagcgcat caacaatatt ttcacctgaa tcaggatatt cttctaatac ctggaatgct
     9901 gttttccctg ggatcgcagt ggtgagtaac catgcatcat caggagtacg gataaaatgc
     9961 ttgatggtcg gaagaggcat aaattccgtc agccagttta gtctgaccat ctcatctgta
    10021 acatcattgg caacgctacc tttgccatgt ttcagaaaca actctggcgc atcgggcttc
    10081 ccatacaatc ggtagattgt cgcacctgat tgcccgacat tatcgcgagc ccatttatac
    10141 ccatataaat cagcatccat gttggaattt aatcgcggcc ttgagcaaga cgtttcccgt
    10201 tgaatatggc tcataacacc ccttgtatta ctgtttatgt aagcagacag ttttattgtt
    10261 catgatgata tatttttatc ttgtgcaatg taacatcaga gattttgaga cacaacgtgg
    10321 ctttgttgaa taaatcgaac ttttgctgag ttgaaggatc agatcacgca tcttcccgac
    10381 aacgcagacc gttccgtggc aaagcaaaag ttcaaaatca ccaactggtc cacctacaac
    10441 aaagctctca tcaaccgtgg ctccctcact ttctggctgg atgatggggc gattcaggcg
    10501 atccccatcc aacagcccgc cgtcgagcgg gcttttttat ccccggaagc ctgtggatag
    10561 agggtagtta tccacgtgaa accgctaatg ccccgcaaag ccttgattca cggggctttc
    10621 cggcccgctc caaaaactat ccacgtgaaa tcgctaatca gggtacgtga aatcgctaat
    10681 cggagtacgt gaaatcgcta ataaggtcac gtgaaatcgc taatcaaaaa ggcacgtgag
    10741 aacgctaata gccctttcag atcaacagct tgcaaacacc cctcgctccg gcaagtagtt
    10801 acagcaagta gtatgttcaa ttagcttttc aattatgaat atatatatca attattggtc
    10861 gcccttggct tgtggacaat gcgctacgcg caccggctcc gcccgtggac aaccgcaagc
    10921 ggttgcccac cgtcgagcgc ctttgcccac aacccggcgg ccggccgcaa cagatcgttt
    10981 tataaatttt tttttttgaa aaagaaaaag cccgaaaggc ggcaacctct cgggcttctg
    11041 gatttccgat ccccggaatt agatcttggc aggatatatt gtggtgtaac gttatcgatc
    11101 tggattttag tactggattt tggttttagg aattagaaat tttattgata gaagtatttt
    11161 acaaatacaa atacatacta agggtttctt atatgctcaa cacatgagcg aaaccctata
    11221 agaaccctaa tttcccttat cgggaaacta ctcacacatt atttatggag aaaatagaga
    11281 gagatagatt tgtagagaga gactggtgat ttcagcgtac cgaattaatt cgagctcggt
    11341 acccctggat tttggtttta ggaattagaa attttattga tagaagtatt ttacaaatac
    11401 aaatacatac taagggtttc ttatatgctc aacacatgag cgaaacccta taagaaccct
    11461 aattccctta tctgggaact actcacacat tattatagag agagatagat ttgtagagag
    11521 agactggtga tttcagcggg ggattcagcg ccgacgggac gtaaacaaag gacgtcccgc
    11581 gaaggatcta aagccagcaa aagtcccatg gtcttataaa aatgcatagc tttaggaggg
    11641 gagcagagaa cttgaaagca tcttcctgtt agtctttctt ctcgtagact tcaaacttat
    11701 acttgatgcc tttttcctcc tggacctcag agaggacgcc tgggtattct gggagaagtt
    11761 tatatttccc caaatcaatt tctgggaaaa acgtgtcact ttcaaattcc tgcatgatcc
    11821 ttgtcacaaa gagtctgagg tggcctggtt gattcatggc ttcctggtaa acagaactgc
    11881 ctccgactat ccaaaccatg tctactttac ttgccaattc cggttgttca ataagtctta
    11941 aggcatcatc caaacttttg gcaagaaaat gagctcctcg tggtggttct ttgagttctc
    12001 tactgagaac tatattaatt ctgtccttta aaggtcgatt cttctcagga atggagaacc
    12061 aggttttcct acccataatc accagattct gtttaccttc cactgaagag gttgtggtca
    12121 ttctttggaa gtacttgaac tcgttcctga gcggaggcca gggtcggtct ccgttcttgc
    12181 caatccccat attttgggac acggcgacga tgcagttcaa tggtcgaacc atgatggcaa
    12241 attctagggg tacccagctt gggctgtcct ctccaaatga aatgaacttc cttatataga
    12301 ggaagggtct tgcgaaggat agtgggattg tgcgtcatcc cttacgtcag tggagatgtc
    12361 acatcaatcc acttgctttg aagacgtggt tggaacgtct tctttttcca cgatgctcct
    12421 cgtgggtggg ggtccatctt tgggaccact gtcggcagag gcatcttgaa tgatagcctt
    12481 tcctttatcg caatgatggc atttgtagga gccaccttcc ttttctactg tcctttcgat
    12541 gaagtgacag atagctgggc aatggaatcc gaggaggttt cccgaaatta ccctttgttg
    12601 aaaagtctca atagcccttt ggtcttctga gactgtatct ttgacatttt tggagtaggg
    12661 gtacgataac attaacgctt acaatttcca ttcgccattc aggctgcgca actgttggga
    12721 agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc
    12781 aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc
    12841 cagtgagcgc gcgtaatacg actcactata gggcgaattg ggtaccgggc ccgtatggtt
    12901 aattaagaca caggcgcgcc
//
