LOCUS       40924_22622        9680 bp DNA     circular SYN 13-MAY-2021
DEFINITION  Expresses nuclear tdTomato from auxin-dependent DR5v2 (TGTCGG) 
            promoter and nuclear 3xGFP from the DR5 (TGTCTC) promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9680)
  AUTHORS   Liao CY, Smet W, Brunoud G, Yoshida S, Vernoux T, Weijers D
  TITLE     Reporters for sensitive and quantitative measurement of auxin 
            response.
  JOURNAL   Nat Methods. 2015 Mar;12(3):207-10. doi: 10.1038/nmeth.3279. Epub 
            2015 Feb 2.
  PUBMED    25643149
REFERENCE   2  (bases 1 to 9680)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 9680)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.3279"; journalName: "Nat Methods"; date: "2015-03"; 
            volume: "12"; issue: "3"; pages: "207-10"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9680
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        157..202
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     misc_feature    213..268
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     CDS             315..335
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             336..1763
                     /codon_start=1
                     /label=tdTomato
                     /note="tandem dimeric (pseudo-monomeric) derivative of
                     DsRed (Shaner et al., 2004)"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     promoter        2192..2237
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     misc_feature    2248..2303
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     CDS             2325..2345
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2346..3062
                     /codon_start=1
                     /label=EGFP
                     /note="enhanced GFP"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             3069..3788
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             3069..3785
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             3809..4516
                     /codon_start=1
                     /label=EGFP
                     /note="enhanced GFP"
                     /translation="MSSKGEELFTGVVPILVELDGDGNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITHGMDE"
     promoter        complement(4869..4887)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4908..4924)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(4932..4948)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4956..4986)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(5001..5022)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     primer_bind     complement(5139..5156)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    5261..5285
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      complement(5376..5964)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6138..6950)
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     rep_origin      7241..7676
                     /label=pSa ori
                     /note="origin of replication from bacterial plasmid pSa"
     misc_feature    7803..7825
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA
                     (truncated)"
     polyA_signal    7833..8009
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(8408..8968)
                     /codon_start=1
                     /label=DHFR
                     /note="mouse dihydrofolate reductase"
                     /translation="MVRPLNCIVAVSQNMGIGKNGDRPWPPLRNEFKYFQRMTTTSSVE
                     GKQNLVIMGRKTWFSIPEKNRPLKDRINIVLSRELKEPPRGAHFLAKSLDDALRLIEQP
                     ELASKVDMVWIVGGSSVYQEAMNQPGHLRLFVTRIMQEFESDTFFPEIDLGKYKLLPEY
                     PGVLSEVQEEKGIKYKFEVYEKKD"
     promoter        complement(9001..9346)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     9564..9580
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        9590..9608
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ccgacaaaag gccgacaaaa ggccgacaaa aggccgacaa aaggccgaca aaaggccgac
       61 aaaaggccga caaaaggccg acaaaaggcc gacaaaaggc cgacaaaagg gggcaggcct
      121 cgataagctt gatatcgaat taattcctgc agccccgcaa gacccttcct ctatataagg
      181 aagttcattt catttggaga ggtattttta caacaattac caacaacaac aaacaacaaa
      241 caacattaca attactattt acaattacaa ttacagggga tccgaaatcc aactccatac
      301 tgaagcttat ggctccaaag aagaagagaa aggtcatggt gagcaagggc gaggaggtca
      361 tcaaagagtt catgcgcttc aaggtgcgca tggagggctc catgaacggc cacgagttcg
      421 agatcgaggg cgagggcgag ggccgcccct acgagggcac ccagaccgcc aagctgaagg
      481 tgaccaaggg cggccccctg cccttcgcct gggacatcct gtccccccag ttcatgtacg
      541 gctccaaggc gtacgtgaag caccccgccg acatccccga ttacaagaag ctgtccttcc
      601 ccgagggctt caagtgggag cgcgtgatga acttcgagga cggcggtctg gtgaccgtga
      661 cccaggactc ctccctgcag gacggcacgc tgatctacaa ggtgaagatg cgcggcacca
      721 acttcccccc cgacggcccc gtaatgcaga agaagaccat gggctgggag gcctccaccg
      781 agcgcctgta cccccgcgac ggcgtgctga agggcgagat ccaccaggcc ctgaagctga
      841 aggacggcgg ccactacctg gtggagttca agaccatcta catggccaag aagcccgtgc
      901 aactgcccgg ctactactac gtggacacca agctggacat cacctcccac aacgaggact
      961 acaccatcgt ggaacagtac gagcgctccg agggccgcca ccacctgttc ctggggcatg
     1021 gcaccggcag caccggcagc ggcagctccg gcaccgcctc ctccgaggac aacaacatgg
     1081 ccgtcatcaa agagttcatg cgcttcaagg tgcgcatgga gggctccatg aacggccacg
     1141 agttcgagat cgagggcgag ggcgagggcc gcccctacga gggcacccag accgccaagc
     1201 tgaaggtgac caagggcggc cccctgccct tcgcctggga catcctgtcc ccccagttca
     1261 tgtacggctc caaggcgtac gtgaagcacc ccgccgacat ccccgattac aagaagctgt
     1321 ccttccccga gggcttcaag tgggagcgcg tgatgaactt cgaggacggc ggtctggtga
     1381 ccgtgaccca ggactcctcc ctgcaggacg gcacgctgat ctacaaggtg aagatgcgcg
     1441 gcaccaactt cccccccgac ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct
     1501 ccaccgagcg cctgtacccc cgcgacggcg tgctgaaggg cgagatccac caggccctga
     1561 agctgaagga cggcggccac tacctggtgg agttcaagac catctacatg gccaagaagc
     1621 ccgtgcaact gcccggctac tactacgtgg acaccaagct ggacatcacc tcccacaacg
     1681 aggactacac catcgtggaa cagtacgagc gctccgaggg ccgccaccac ctgttcctgt
     1741 acggcatgga cgagctgtac aagtagctcg agagatccga tccccgatcg ttcaaacatt
     1801 tggcaataaa gtttcttaag attgaatcct gttgccggtc ttgcgatgat tatcatataa
     1861 tttctgttga attacgttaa gcatgtaata attaacatgt aatgcatgac gttatttatg
     1921 agatgggttt ttatgattag agtcccgcaa ttatacattt aatacgcgat agaaaacaaa
     1981 atatagcgcg caaactagga taaattatcg cgcgcggtgt catctatgtg aattcgagac
     2041 aaaagggaga caaaagggag acaaaaggga gacaaaaggg agacaaaagg gagacaaaag
     2101 ggagacaaaa gggagacaaa agggagacaa aagggagaca aaagggggca ggcctcgata
     2161 agcttgatat cgaattaatt cctgcagccc cgcaagaccc ttcctctata taaggaagtt
     2221 catttcattt ggagaggtat ttttacaaca attaccaaca acaacaaaca acaaacaaca
     2281 ttacaattac tatttacaat tacaattaca ggggatccat ggctccaaag aagaagagaa
     2341 aggtcatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc
     2401 tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca
     2461 cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc
     2521 ccaccctcgt gaccaccctg acctacggcg tgcagtgctt cagccgctac cccgaccaca
     2581 tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca
     2641 tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca
     2701 ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg
     2761 ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcatggcc gacaagcaga
     2821 agaacggcat caaggtgaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc
     2881 tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca
     2941 accactacct gagcacccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca
     3001 tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca
     3061 agagatccat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg
     3121 agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg
     3181 ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct
     3241 ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc taccccgacc
     3301 acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca
     3361 ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg
     3421 acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc
     3481 tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc
     3541 agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc
     3601 agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg
     3661 acaaccacta cctgagcacc cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc
     3721 acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt
     3781 acaagtaaag atccaaggag atataaccat gagtagtaaa ggagaagaac ttttcactgg
     3841 agttgtccca attcttgttg aattagatgg tgatggtaat gggcacaaat tttctgtcag
     3901 tggagagggt gaaggtgatg caacatacgg aaaacttacc cttaaattta tttgcactac
     3961 tggaaaacta cctgttccat ggccaacact tgtcactacg ttaacttatg gagtacaatg
     4021 cttttcaaga tacccagatc atatgaagcg gcacgacttc ttcaagagcg ccatgcctga
     4081 gggatacgtg caggagagga ccatcttctt caaggacgac gggaactaca agacacgtgc
     4141 tgaagtcaag tttgagggag acaccctcgt caacaggatc gagcttaagg gaatcgattt
     4201 caaggaggac ggaaacatcc tcggccacaa gttggaatac aactacaact cccacaacgt
     4261 atacatcatg gcagacaaac aaaagaatgg aatcaaagtc aacttcaaaa ttagacacaa
     4321 cattgaagat ggaagcgttc aactagcaga ccattatcaa caaaatactc caattggcga
     4381 tggccctgtc cttttaccag acaaccatta cctgtccaca caatctgccc tttcgaaaga
     4441 tcccaacgaa aagagagacc acatggtcct tcttgagttt gtaacagctg ctgggattac
     4501 acatggcatg gatgaataca aatcttacgt tactgttcca aacagaacta gaacttacat
     4561 caagtaatct agatccgatc cccgatcgtt caaacatttg gcaataaagt ttcttaagat
     4621 tgaatcctgt tgccggtctt gcgatgatta tcatataatt tctgttgaat tacgttaagc
     4681 atgtaataat taacatgtaa tgcatgacgt tatttatgag atgggttttt atgattagag
     4741 tcccgcaatt atacatttaa tacgcgatag aaaacaaaat atagcgcgca aactaggata
     4801 aattatcgcg cgcggtgtca tctatgtcta gagcggccgc caccgcggtg gagctccagc
     4861 ttttgttccc tttagtgagg gttaattgcg cgcttggcgt aatcatggtc atagctgttt
     4921 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
     4981 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
     5041 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
     5101 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
     5161 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     5221 acagaatcag gggataacgc aggaaagaac atgaaggcct tgacaggata tattggcggg
     5281 taaactaagt cgctgtatgt gtttgtttga gatctcatgt gagcaaaagg ccagcaaaag
     5341 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     5401 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     5461 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     5521 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     5581 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     5641 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     5701 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     5761 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
     5821 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaagaagagt tggtagctct
     5881 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     5941 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     6001 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     6061 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgtgtaa
     6121 cattggtcta gtgattagaa aaactcatcg agcatcaaat gaaactgcaa tttattcata
     6181 tcaggattat caataccata tttttgaaaa agccgtttct gtaatgaagg agaaaactca
     6241 ccgaggcagt tccataggat ggcaagatcc tggtatcggt ctgcgattcc gactcgtcca
     6301 acatcaatac aacctattaa tttcccctcg tcaaaaataa ggttatcaag tgagaaatca
     6361 ccatgagtga cgactgaatc cggtgagaat ggcaaaagtt tatgcatttc tttccagact
     6421 tgttcaacag gccagccatt acgctcgtca tcaaaatcac tcgcatcaac caaaccgtta
     6481 ttcattcgtg attgcgcctg agcgagacga aatacgcgat cgctgttaaa aggacaatta
     6541 caaacaggaa tcgaatgcaa ccggcgcagg aacactgcca gcgcatcaac aatattttca
     6601 cctgaatcag gatattcttc taatacctgg aatgctgttt tccctgggat cgcagtggtg
     6661 agtaaccatg catcatcagg agtacggata aaatgcttga tggtcggaag aggcataaat
     6721 tccgtcagcc agtttagtct gaccatctca tctgtaacat cattggcaac gctacctttg
     6781 ccatgtttca gaaacaactc tggcgcatcg ggcttcccat acaatcggta gattgtcgca
     6841 cctgattgcc cgacattatc gcgagcccat ttatacccat ataaatcagc atccatgttg
     6901 gaatttaatc gcggccttga gcaagacgtt tcccgttgaa tatggctcat aacacccctt
     6961 gtattactgt ttatgtaagc agacagtttt attgttcatg atgatatatt tttatcttgt
     7021 gcaatgtaac atcagagatt ttgagacaca acgtggcttt gttgaataaa tcgaactttt
     7081 gctgagttga aggatcagat cacgcatctt cccgacaacg cagaccgttc cgtggcaaag
     7141 caaaagttca aaatcaccaa ctggtccacc tacaacaaag ctctcatcaa ccgtggctcc
     7201 ctcactttct ggctggatga tggggcgatt caggcgatcc ccatccaaca gcccgccgtc
     7261 gagcgggctt ttttatcccc ggaagcctgt ggatagaggg tagttatcca cgtgaaaccg
     7321 ctaatgcccc gcaaagcctt gattcacggg gctttccggc ccgctccaaa aactatccac
     7381 gtgaaatcgc taatcagggt acgtgaaatc gctaatcgga gtacgtgaaa tcgctaataa
     7441 ggtcacgtga aatcgctaat caaaaaggca cgtgagaacg ctaatagccc tttcagatca
     7501 acagcttgca aacacccctc gctccggcaa gtagttacag caagtagtat gttcaattag
     7561 cttttcaatt atgaatatat atatcaatta ttggtcgccc ttggcttgtg gacaatgcgc
     7621 tacgcgcacc ggctccgccc gtggacaacc gcaagcggtt gcccaccgtc gagcgccttt
     7681 gcccacaacc cggcggccgg ccgcaacaga tcgttttata aatttttttt tttgaaaaag
     7741 aaaaagcccg aaaggcggca acctctcggg cttctggatt tccgatcccc ggaattagat
     7801 cttggcagga tatattgtgg tgtaacgtta tcgatctgga ttttagtact ggattttggt
     7861 tttaggaatt agaaatttta ttgatagaag tattttacaa atacaaatac atactaaggg
     7921 tttcttatat gctcaacaca tgagcgaaac cctataagaa ccctaatttc ccttatcggg
     7981 aaactactca cacattattt atggagaaaa tagagagaga tagatttgta gagagagact
     8041 ggtgatttca gcgtaccgaa ttaattcgag ctcggtaccc ctggattttg gttttaggaa
     8101 ttagaaattt tattgataga agtattttac aaatacaaat acatactaag ggtttcttat
     8161 atgctcaaca catgagcgaa accctataag aaccctaatt cccttatctg ggaactactc
     8221 acacattatt atagagagag atagatttgt agagagagac tggtgatttc agcgggggat
     8281 tcagcgccga cgggacgtaa acaaaggacg tcccgcgaag gatctaaagc cagcaaaagt
     8341 cccatggtct tataaaaatg catagcttta ggaggggagc agagaacttg aaagcatctt
     8401 cctgttagtc tttcttctcg tagacttcaa acttatactt gatgcctttt tcctcctgga
     8461 cctcagagag gacgcctggg tattctggga gaagtttata tttccccaaa tcaatttctg
     8521 ggaaaaacgt gtcactttca aattcctgca tgatccttgt cacaaagagt ctgaggtggc
     8581 ctggttgatt catggcttcc tggtaaacag aactgcctcc gactatccaa accatgtcta
     8641 ctttacttgc caattccggt tgttcaataa gtcttaaggc atcatccaaa cttttggcaa
     8701 gaaaatgagc tcctcgtggt ggttctttga gttctctact gagaactata ttaattctgt
     8761 cctttaaagg tcgattcttc tcaggaatgg agaaccaggt tttcctaccc ataatcacca
     8821 gattctgttt accttccact gaagaggttg tggtcattct ttggaagtac ttgaactcgt
     8881 tcctgagcgg aggccagggt cggtctccgt tcttgccaat ccccatattt tgggacacgg
     8941 cgacgatgca gttcaatggt cgaaccatga tggcaaattc taggggtacc cagcttgggc
     9001 tgtcctctcc aaatgaaatg aacttcctta tatagaggaa gggtcttgcg aaggatagtg
     9061 ggattgtgcg tcatccctta cgtcagtgga gatgtcacat caatccactt gctttgaaga
     9121 cgtggttgga acgtcttctt tttccacgat gctcctcgtg ggtgggggtc catctttggg
     9181 accactgtcg gcagaggcat cttgaatgat agcctttcct ttatcgcaat gatggcattt
     9241 gtaggagcca ccttcctttt ctactgtcct ttcgatgaag tgacagatag ctgggcaatg
     9301 gaatccgagg aggtttcccg aaattaccct ttgttgaaaa gtctcaatag ccctttggtc
     9361 ttctgagact gtatctttga catttttgga gtaggggtac gataacatta acgcttacaa
     9421 tttccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc
     9481 gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc
     9541 agggttttcc cagtcacgac gttgtaaaac gacggccagt gagcgcgcgt aatacgactc
     9601 actatagggc gaattgggta ccgggcccgt atggttaatt aagacacagg cgcgcctagt
     9661 tggaatagga tttcgaattc
//
