LOCUS       40924_22617        3304 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Improved compact Agrobacterium binary vector with a 
            kanamycin-resistance gene. Also known as pGreenII 0000.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3304)
  AUTHORS   Hellens RP, Edwards EA, Leyland NR, Bean S, Mullineaux PM.
  TITLE     pGreen: a versatile and flexible binary Ti vector for 
            Agrobacterium-mediated plant transformation.
  JOURNAL   Plant Mol. Biol. 2000;42:819-32.
  PUBMED    10890530
REFERENCE   2  (bases 1 to 3304)
  AUTHORS   pGreen website
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 3304)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Mol. 
            Biol."; date: "2000"; volume: "42"; pages: "819-32"
COMMENT     SGRef: number: 2; type: "Journal Article"
COMMENT     This plasmid is more stable in E. coli than the original pGreen. It 
            must be transformed into an Agrobacterium strain that also carries a
            vector such as pSoup.
FEATURES             Location/Qualifiers
     source          1..3304
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    542..564
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA
                     (truncated)"
     primer_bind     733..749
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        756..774
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    complement(783..890)
                     /label=MCS
                     /note="pBluescript multiple cloning site"
     promoter        complement(903..921)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(942..958)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(966..982)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(990..1021)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1036..1057)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     misc_feature    1296..1320
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      complement(1411..1999)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2173..2985)
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANVVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     rep_origin      3276..3304
                     /label=pSa ori
                     /note="origin of replication from bacterial plasmid pSa"
ORIGIN
        1 tttttatccc cggaagcctg tggatagagg gtagttatcc acgtgaaacc gctaatgccc
       61 cgcaaagcct tgattcacgg ggctttccgg cccgctccaa aaactatcca cgtgaaatcg
      121 ctaatcaggg tacgtgaaat cgctaatcgg agtacgtgaa atcgctaata aggtcacgtg
      181 aaatcgctaa tcaaaaaggc acgtgagaac gctaatagcc ctttcagatc aacagcttgc
      241 aaacacccct cgctccggca agtagttaca gcaagtagta tgttcaatta gcttttcaat
      301 tatgaatata tatatcaatt attggtcgcc cttggcttgt ggacaatgcg ctacgcgcac
      361 cggctccgcc cgtggacaac cgcaagcggt tgcccaccgt cgagcgccag cgcctttgcc
      421 cacaacccgg cggccggccg caacagatcg ttttataaat tttttttttt gaaaaagaaa
      481 aagcccgaaa ggcggcaacc tctcgggctt ctggatttcc gatccccgga attagagatc
      541 ttggcaggat atattgtggt gtaacgttaa cattaacgtt tacaatttcg cgccattcgc
      601 cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc
      661 agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc
      721 agtcacgacg ttgtaaaacg acggccagtg aattgtaata cgactcacta tagggcgaat
      781 tgggtaccgg gccccccctc gaggtcgacg gtatcgataa gcttgatatc gaattcctgc
      841 agcccggggg atccactagt tctagagcgg ccgccaccgc ggtggagctc cagcttttgt
      901 tccctttagt gagggttaat tccgagcttg gcgtaatcat ggtcatagct gtttcctgtg
      961 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaaghca taaagtgtaa
     1021 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
     1081 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
     1141 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     1201 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     1261 atcaggggat aacgcaggaa agaacatgaa ggccttgaca ggatatattg gcgggtaaac
     1321 taagtcgctg tatgtgtttg tttgagatct catgtgagca aaaggccagc aaaaggccag
     1381 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     1441 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     1501 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     1561 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     1621 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     1681 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     1741 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     1801 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     1861 tggtatctgc gctctgctga agccagttac cttcggaaga agagttggta gctcttgatc
     1921 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     1981 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     2041 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     2101 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg tgtaacattg
     2161 gtctagtgat tagaaaaact catcgagcat caaatgaaac tgcaatttat tcatatcagg
     2221 attatcaata ccatattttt gaaaaagccg tttctgtaat gaaggagaaa actcaccgag
     2281 gcagttccat aggatggcaa gatcctggta tcggtctgcg attccgactc gtccaacatc
     2341 aatacaacct attaatttcc cctcgtcaaa aataaggtta tcaagtgaga aatcaccatg
     2401 agtgacgact gaatccggtg agaatggcaa aagtttatgc atttctttcc agacttgttc
     2461 aacaggccag ccattacgct cgtcatcaaa atcactcgca tcaaccaaac cgttattcat
     2521 tcgtgattgc gcctgagcga gacgaaatac gcgatcgctg ttaaaaggac aattacaaac
     2581 aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca tcaacaatat tttcacctga
     2641 atcaggatat tcttctaata cctggaatgc tgttttccct gggatcgcag tggtgagtaa
     2701 ccatgcatca tcaggagtac ggataaaatg cttgatggtc ggaagaggca taaattccgt
     2761 cagccagttt agtctgacca tctcatctgt aacaacattg gcaacgctac ctttgccatg
     2821 tttcagaaac aactctggcg catcgggctt cccatacaat cggtagattg tcgcacctga
     2881 ttgcccgaca ttatcgcgag cccatttata cccatataaa tcagcatcca tgttggaatt
     2941 taatcgcggc cttgagcaag acgtttcccg ttgaatatgg ctcataacac cccttgtatt
     3001 actgtttatg taagcagaca gttttattgt tcatgatgat atatttttat cttgtgcaat
     3061 gtaacatcag agattttgag acacaacgtg gctttgttga ataaatcgaa cttttgctga
     3121 gttgaaggat cagatcacgc atcttcccga caacgcagac cgttccgtgg caaagcaaaa
     3181 gttcaaaatc accaactggt ccacctacaa caaagctctc atcaaccgtg gctccctcac
     3241 tttctggctg gatgatgggg cgattcaggc gatccccatc caacagcccg ccgtcgagcg
     3301 ggct
//
