LOCUS       40924_22612        3329 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Binary cloning vector pGreenII-ArrayMCS, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3329)
  AUTHORS   Shiboleth YM.
  TITLE     pGreenII-ArrayMCS binary cloning vector
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3329)
  AUTHORS   Shiboleth YM.
  TITLE     Direct Submission
  JOURNAL   
REFERENCE   3  (bases 1 to 3329)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3329)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (10-JUN-2011) Department of Molecular, Cellular, "
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3329
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      26..95
                     /label=pSa Ori
                     /note="pSa Ori"
     misc_feature    542..564
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA
                     (truncated)"
     regulatory      579..584
                     /label=Escherichia coli lac promoter RNApol binding site
                     /note="Escherichia coli lac promoter RNApol binding site"
                     /regulatory_class="minus_35_signal"
     primer_bind     733..749
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        756..774
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    788..914
                     /label=multiple cloning site
                     /note="multiple cloning site"
     promoter        complement(928..946)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(967..983)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(991..1007)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1015..1046)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1061..1082)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     misc_feature    1321..1345
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      complement(1436..2024)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2198..3010)
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     rep_origin      3301..3329
                     /label=pSa ori
                     /note="origin of replication from bacterial plasmid pSa"
ORIGIN
        1 tttttatccc cggaagcctg tggatagagg gtagttatcc acgtgaaacc gctaatgccc
       61 cgcaaagcct tgattcacgg ggctttccgg cccgctccaa aaactatcca cgtgaaatcg
      121 ctaatcaggg tacgtgaaat cgctaatcgg agtacgtgaa atcgctaata aggtcacgtg
      181 aaatcgctaa tcaaaaaggc acgtgagaac gctaatagcc ctttcagatc aacagcttgc
      241 aaacacccct cgctccggca agtagttaca gcaagtagta tgttcaatta gcttttcaat
      301 tatgaatata tatatcaatt attggtcgcc cttggcttgt ggacaatgcg ctacgcgcac
      361 cggctccgcc cgtggacaac cgcaagcggt tgcccaccgt cgagcgccag cgcctttgcc
      421 cacaacccgg cggccggccg caacagatcg ttttataaat tttttttttt gaaaaagaaa
      481 aagcccgaaa ggcggcaacc tctcgggctt ctggatttcc gatccccgga attagagatc
      541 ttggcaggat atattgtggt gtaacgttaa cattaacgtt tacaatttcg cgccattcgc
      601 cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc
      661 agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc
      721 agtcacgacg ttgtaaaacg acggccagtg aattgtaata cgactcacta tagggcgaat
      781 tgggtaccgg cgcgccgtcg acggtatcga tggatcctga tgaattctag ggataacagg
      841 gtaatctcga gttaaaagct taccggttag tgcggccgct ggcaaacagc tattatgggt
      901 attatgggtg agctccagct tttgttccct ttagtgaggg ttaattccga gcttggcgta
      961 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
     1021 acgagccgga agncataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
     1081 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
     1141 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
     1201 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     1261 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgaaggcct
     1321 tgacaggata tattggcggg taaactaagt cgctgtatgt gtttgtttga gatctcatgt
     1381 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     1441 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     1501 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     1561 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     1621 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     1681 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     1741 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     1801 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     1861 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     1921 gaagaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     1981 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     2041 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     2101 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     2161 tctaaagtat atatgtgtaa cattggtcta gtgattagaa aaactcatcg agcatcaaat
     2221 gaaactgcaa tttattcata tcaggattat caataccata tttttgaaaa agccgtttct
     2281 gtaatgaagg agaaaactca ccgaggcagt tccataggat ggcaagatcc tggtatcggt
     2341 ctgcgattcc gactcgtcca acatcaatac aacctattaa tttcccctcg tcaaaaataa
     2401 ggttatcaag tgagaaatca ccatgagtga cgactgaatc cggtgagaat ggcaaaagtt
     2461 tatgcatttc tttccagact tgttcaacag gccagccatt acgctcgtca tcaaaatcac
     2521 tcgcatcaac caaaccgtta ttcattcgtg attgcgcctg agcgagacga aatacgcgat
     2581 cgctgttaaa aggacaatta caaacaggaa tcgaatgcaa ccggcgcagg aacactgcca
     2641 gcgcatcaac aatattttca cctgaatcag gatattcttc taatacctgg aatgctgttt
     2701 tccctgggat cgcagtggtg agtaaccatg catcatcagg agtacggata aaatgcttga
     2761 tggtcggaag aggcataaat tccgtcagcc agtttagtct gaccatctca tctgtaacaa
     2821 cattggcaac gctacctttg ccatgtttca gaaacaactc tggcgcatcg ggcttcccat
     2881 acaatcggta gattgtcgca cctgattgcc cgacattatc gcgagcccat ttatacccat
     2941 ataaatcagc atccatgttg gaatttaatc gcggccttga gcaagacgtt tcccgttgaa
     3001 tatggctcat aacacccctt gtattactgt ttatgtaagc agacagtttt attgttcatg
     3061 atgatatatt tttatcttgt gcaatgtaac atcagagatt ttgagacaca acgtggcttt
     3121 gttgaataaa tcgaactttt gctgagttga aggatcagat cacgcatctt cccgacaacg
     3181 cagaccgttc cgtggcaaag caaaagttca aaatcaccaa ctggtccacc tacaacaaag
     3241 ctctcatcaa ccgtggctcc ctcactttct ggctggatga tggggcgatt caggcgatcc
     3301 ccatccaaca gcccgccgtc gagcgggct
//
