LOCUS       V013691                 4590 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V013691
VERSION     V013691
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4590)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4590
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    542..564
                     /label="LB T-DNA repeat"
                     /note="left border repeat from nopaline C58 T-DNA
                     (truncated)"
     promoter        638..982
                     /label="CaMV 35S promoter"
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     1016..1032
                     /label="SK primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             1040..1840
                     /gene="luz"
                     /label="Luciferase"
                     /note="Luciferase from Neonothopanus nambi. Accession#:
                     A0A3G9JYH7"
     terminator      1865..2572
                     /label="OCS terminator"
                     /note="octopine synthase terminator"
     misc_feature    2582..2606
                     /label="RB T-DNA repeat"
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      complement(2697..3285)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(3459..4271)
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
     rep_origin      4562..4590
                     /label="pSa ori"
                     /note="origin of replication from bacterial plasmid pSa"
ORIGIN
        1 tttttatccc cggaagcctg tggatagagg gtagttatcc acgtgaaacc gctaatgccc
       61 cgcaaagcct tgattcacgg ggctttccgg cccgctccaa aaactatcca cgtgaaatcg
      121 ctaatcaggg tacgtgaaat cgctaatcgg agtacgtgaa atcgctaata aggtcacgtg
      181 aaatcgctaa tcaaaaaggc acgtgagaac gctaatagcc ctttcagatc aacagcttgc
      241 aaacacccct cgctccggca agtagttaca gcaagtagta tgttcaatta gcttttcaat
      301 tatgaatata tatatcaatt attggtcgcc cttggcttgt ggacaatgcg ctacgcgcac
      361 cggctccgcc cgtggacaac cgcaagcggt tgcccaccgt cgagcgccag cgcctttgcc
      421 cacaacccgg cggccggccg caacagatcg ttttataaat tttttttttt gaaaaagaaa
      481 aagcccgaaa ggcggcaacc tctcgggctt ctggatttcc gatccccgga attagagatc
      541 ttggcaggat atattgtggt gtaacgttat cagcttggta cgtacccccc tactccaaaa
      601 atgtcaaaga tacagtctca gaagaccaaa gggctattga gacttttcaa caaagggtaa
      661 tttcgggaaa cctcctcgga ttccattgcc cagctatctg tcacttcatc gaaaggacag
      721 tagaaaagga aggtggctcc tacaaatgcc atcattgcga taaaggaaag gctatcattc
      781 aagatgcctc tgccgacagt ggtcccaaag atggaccccc acccacgagg agcatcgtgg
      841 aaaaagaaga cgttccaacc acgtcttcaa agcaagtgga ttgatgtgac atctccactg
      901 acgtaaggga tgacgcacaa tcccactatc cttcgcaaga cccttcctct atataaggaa
      961 gtcatttcat ttggagagga cagcccaagc tgagctccac cgcggtggcg gccgctctag
     1021 aactagtgga tccgccacca tgcgtataaa tatctctctg tctagcttat tcgagagatt
     1081 aagcaaactc agctctcgta gcatagctat tacatgtggt gtcgtcctcg cctctgctat
     1141 tgcttttccc atcatacgac gagattatca gacgttctta gaggttggtc ctagctatgc
     1201 ccctcaaaat tttcgaggct acattatcgt ttgcgtactc agcttgttcc gacaagagca
     1261 aaaagggttg gcaatctacg acagactccc agagaagaga agatggctgg ccgatctccc
     1321 atttcgtgaa ggtactagac ctagtataac atcacacata atacagagac agcgaactca
     1381 acttgtagac caagagtttg ccacacgaga actgatagac aaagttattc ccagggtgca
     1441 agcacgacat acagataaaa catttttgag cacgagcaag tttgaatttc atgctaaagc
     1501 cattttcctg ttgccaagta ttcctataaa cgacccctta aacataccta gtcatgatac
     1561 ggttcgtaga acgaaacgag agattgcaca catgcatgac taccacgact gtactcttca
     1621 cttagctctt gcagctcaag atggaaaaga agtcctgaaa aaaggctggg gccaacgtca
     1681 tccgcttgcc gggccgggtg tacctgggcc gccgacagaa tggactttct tatacgcacc
     1741 ccgtaatgag gaagaagcta gggtagtaga aatgattgtc gaggcaagta tcggatacat
     1801 gactaatgat ccggcaggta aaatcgtcga gaatgccaaa tgactcgagg gggggcccgg
     1861 taccctgctt taatgagata tgcgagacgc ctatgatcgc atgatatttg ctttcaattc
     1921 tgttgtgcac gttgtaaaaa acctgagcat gtgtagctca gatccttacc gccggtttcg
     1981 gttcattcta atgaatatat cacccgttac tatcgtattt ttatgaataa tattctccgt
     2041 tcaatttact gattgtaccc tactacttat atgtacaata ttaaaatgaa aacaatatat
     2101 tgtgctgaat aggtttatag cgacatctat gatagagcgc cacaataaca aacaattgcg
     2161 ttttattatt acaaatccaa ttttaaaaaa agcggcagaa ccggtcaaac ctaaaagact
     2221 gattacataa atcttattca aatttcaaaa gtgccccagg ggctagtatc tacgacacac
     2281 cgagcggcga actaataacg ctcactgaag ggaactccgg ttccccgccg gcgcgcatgg
     2341 gtgagattcc ttgaagttga gtattggccg tccgctctac cgaaagttac gggcaccatt
     2401 caacccggtc cagcacggcg gccgggtaac cgacttgctg ccccgagaat tatgcagcat
     2461 ttttttggtg tatgtgggcc ccaaatgaag tgcaggtcaa accttgacag tgacgacaaa
     2521 tcgttgggcg ggtccagggc gaattttgcg acaacatgtc gaggctcagc agcactagcc
     2581 ttgacaggat atattggcgg gtaaactaag tcgctgtatg tgtttgtttg agatctcatg
     2641 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     2701 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     2761 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     2821 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     2881 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     2941 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     3001 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     3061 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     3121 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     3181 ggaagaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     3241 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     3301 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     3361 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     3421 atctaaagta tatatgtgta acattggtct agtgattaga aaaactcatc gagcatcaaa
     3481 tgaaactgca atttattcat atcaggatta tcaataccat atttttgaaa aagccgtttc
     3541 tgtaatgaag gagaaaactc accgaggcag ttccatagga tggcaagatc ctggtatcgg
     3601 tctgcgattc cgactcgtcc aacatcaata caacctatta atttcccctc gtcaaaaata
     3661 aggttatcaa gtgagaaatc accatgagtg acgactgaat ccggtgagaa tggcaaaagt
     3721 ttatgcattt ctttccagac ttgttcaaca ggccagccat tacgctcgtc atcaaaatca
     3781 ctcgcatcaa ccaaaccgtt attcattcgt gattgcgcct gagcgagacg aaatacgcga
     3841 tcgctgttaa aaggacaatt acaaacagga atcgaatgca accggcgcag gaacactgcc
     3901 agcgcatcaa caatattttc acctgaatca ggatattctt ctaatacctg gaatgctgtt
     3961 ttccctggga tcgcagtggt gagtaaccat gcatcatcag gagtacggat aaaatgcttg
     4021 atggtcggaa gaggcataaa ttccgtcagc cagtttagtc tgaccatctc atctgtaaca
     4081 acattggcaa cgctaccttt gccatgtttc agaaacaact ctggcgcatc gggcttccca
     4141 tacaatcggt agattgtcgc acctgattgc ccgacattat cgcgagccca tttataccca
     4201 tataaatcag catccatgtt ggaatttaat cgcggccttg agcaagacgt ttcccgttga
     4261 atatggctca taacacccct tgtattactg tttatgtaag cagacagttt tattgttcat
     4321 gatgatatat ttttatcttg tgcaatgtaa catcagagat tttgagacac aacgtggctt
     4381 tgttgaataa atcgaacttt tgctgagttg aaggatcaga tcacgcatct tcccgacaac
     4441 gcagaccgtt ccgtggcaaa gcaaaagttc aaaatcacca actggtccac ctacaacaaa
     4501 gctctcatca accgtggctc cctcactttc tggctggatg atggggcgat tcaggcgatc
     4561 cccatccaac agcccgccgt cgagcgggct
//
