LOCUS       62056_11880        8883 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8883)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..8883
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    542..564
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA
                     (truncated)"
     promoter        638..982
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     1016..1032
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      6158..6865
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     misc_feature    6875..6899
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      complement(6990..7578)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(7752..8564)
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANVVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     rep_origin      8855..8883
                     /label=pSa ori
                     /note="origin of replication from bacterial plasmid pSa"
ORIGIN
        1 tttttatccc cggaagcctg tggatagagg gtagttatcc acgtgaaacc gctaatgccc
       61 cgcaaagcct tgattcacgg ggctttccgg cccgctccaa aaactatcca cgtgaaatcg
      121 ctaatcaggg tacgtgaaat cgctaatcgg agtacgtgaa atcgctaata aggtcacgtg
      181 aaatcgctaa tcaaaaaggc acgtgagaac gctaatagcc ctttcagatc aacagcttgc
      241 aaacacccct cgctccggca agtagttaca gcaagtagta tgttcaatta gcttttcaat
      301 tatgaatata tatatcaatt attggtcgcc cttggcttgt ggacaatgcg ctacgcgcac
      361 cggctccgcc cgtggacaac cgcaagcggt tgcccaccgt cgagcgccag cgcctttgcc
      421 cacaacccgg cggccggccg caacagatcg ttttataaat tttttttttt gaaaaagaaa
      481 aagcccgaaa ggcggcaacc tctcgggctt ctggatttcc gatccccgga attagagatc
      541 ttggcaggat atattgtggt gtaacgttat cagcttggta cgtacccccc tactccaaaa
      601 atgtcaaaga tacagtctca gaagaccaaa gggctattga gacttttcaa caaagggtaa
      661 tttcgggaaa cctcctcgga ttccattgcc cagctatctg tcacttcatc gaaaggacag
      721 tagaaaagga aggtggctcc tacaaatgcc atcattgcga taaaggaaag gctatcattc
      781 aagatgcctc tgccgacagt ggtcccaaag atggaccccc acccacgagg agcatcgtgg
      841 aaaaagaaga cgttccaacc acgtcttcaa agcaagtgga ttgatgtgac atctccactg
      901 acgtaaggga tgacgcacaa tcccactatc cttcgcaaga cccttcctct atataaggaa
      961 gtcatttcat ttggagagga cagcccaagc tgagctccac cgcggtggcg gccgctctag
     1021 aactagtgga tccgccacca tgaatagtag taaaaaccct ccatccactc tgttagatgt
     1081 ttttctggat accgctagga accttgatac agcatccaga aatgtcctgg agtgcggaga
     1141 acacaggtgg agttacagag agttggacac agtgtcatcc gcattggccc aacaccttcg
     1201 atacactgtg ggtcttagcc ccactgtagc cgtgattagc gagaatcacc cctacatact
     1261 tgcattaatg ctcgccgtat ggaaactcgg cggaacgttc gcaccgatag atgttcattc
     1321 cccagccgaa cttgttgctg gaatgttgaa tattgtaagc ccgtcatgtc ttgtcattcc
     1381 tagctctgat gtgacaaatc agacattggc atgcgatctt aatataccgg ttgttgcatt
     1441 ccacccacat caaagtacaa ttcccgaact caacaagaag tatctcacag attctcagat
     1501 cagtcctgac ctgcccttcc ccgaccctaa tcgacctgct ttgtacttat tcacgtcatc
     1561 tgcaacgtct aggtccaatt tgaagtgcgt gccacttaca cacacattca tattgcgaaa
     1621 ctccttaagt aaacgagcat ggtgcaaaag aatgaggcca gagacagatt ttgatggtat
     1681 tagggtcctg gggtgggccc cctggagcca tgtcctcgct catatgcagg acattggccc
     1741 tcttacatta ttgaatgccg ggtgctacgt atttgccacg accccatcta cctacccgac
     1801 ggagctgaag gatgaccgtg atgttattag ttgcgccgca aatgccgtaa tgtacaaagg
     1861 tgttaaatcc ttcgcatgcc tcccattcgt gcttggggga ctcaaggctt tatgtgagtc
     1921 agagcccagt gttaaggcac aactgcaagt ggaggaacga gcccagcttt tgaagtcact
     1981 ccaacacatg aaaatcctgg aatgcggggg ggcaatgctc gaagtgagtg tggcaagctg
     2041 ggctatagag aataggattc ctatatctat tgggattggt atgacagaga caggcggcgc
     2101 tcttttcgct gggccggtcc aagcaattca aaccggcttt agcagtgaag ataaatttat
     2161 cgaggacgca acctacttgc tggtcaagga cgattacgag agccacgcag aggaggacat
     2221 aaacgagggc gagttagtgg taaagtccag aatgttacct aggggttatt tgggctataa
     2281 cgacccctct ttctctgttg acgatgctgg ttgggttact ttcaaaactg gagatcgata
     2341 ttcagtcact cctgacggca aattctcctg gcttggtcga aatactgatt tcattcagat
     2401 gacgagtggt gagactctcg atccccgtcc gattgagtcc ctcctttgtg agtccagcct
     2461 tatatcacga gcctgtgtca tcggggataa gttcctgaac ggaccagcca cagctgtatg
     2521 cgctattata gaactcgaac caacgactgt tgagaaaggc caggctcaca gcagggatat
     2581 tgcccgaatc tttgccccca tcaatcgaga tcttcctccc ccgttacgta tagcatggag
     2641 tcacgtactt gtacttcagc caagcgagaa aataccgatg acgaaaaagg gtacaatctt
     2701 ccgtaagaaa atcgaacagg ttttcggttc cgctttagga ggcagtagcg gcgacaactc
     2761 acaagcaacc actgacgctt cagtagttag gagggatgag ttgagcaaca cggtcaaaca
     2821 tatcataagc agagttttgg gagtctccga cgatgaactc ctttggacac tgagctttgc
     2881 tgagcttggc atgacgtccg ctcttgctac tagaatagca aatgaattga acgaagttct
     2941 ggtaggtgtg aacctcccca tcaatgcatg ctacattcat gtggacctgc cttctttgtc
     3001 aaatgccgtg tatgcaaagt tagcacacct taaactcccg gataggacgc ccgagccaag
     3061 gaaggcccca gttgaaaatc ccggtggaaa ggaaatcgta atagtaggtc aggcttttag
     3121 gcttccgggg agtataaatg atgtcgcttc tttgagggac gcttttttgg ctagacaagc
     3181 atccagtatt attactgaaa taccgccaga tagatgggac cacgcatcat tttatccaaa
     3241 agacatacga tttaataaag cagggctcgt cgacatcgct aactacgacc acagtttttt
     3301 cggattgaca gccacggagg cactgtacct gtcaccaacg atgcgtttgg cacttgaagt
     3361 gtcctttgaa gccttggaga atgctaatat acccgtgtcc cagttaaagg gctctcaaac
     3421 agctgtatat gtcgcaacca ccgacgacgg gtttgagaca ctgctgaatg ctgaggcagg
     3481 atatgatgca tacacacgat tctatggcac tggtagggcc gcctctacgg ccagtggacg
     3541 aattagttat ttactcgacg tacacggccc ttcaattacc gtagacactg cttgttcagg
     3601 cggtgctgtt tgcatagacc aggccataga ctacctccaa tcctctagcg cagctgatac
     3661 cgccataatc tgcgccagca acactcactg ctggccaggt tccttcatgt ttctgagtgc
     3721 tcaaggtatg gtaagttccg gggggcgatg tgcaactttt acgacggatg ccgacggcta
     3781 tgtaccctct gaaggtgctg tggcatttat cctcaaaacc cgagaagcag ctatgagaga
     3841 taaagatact atcttggcaa ctatcaaagc aacgcagatt agtcataatg gtcgttccca
     3901 aggtttagtc gcaccaaacg tcaattcaca agctgattta cacagaagct tattgcagaa
     3961 agctggtttg agtccagccg acattcactt catcgaagct cacgggacag gaacgtcact
     4021 cggtgacctt agtgaaatac aagcaattaa tgatgcctac acgtcatctc agcctcgaac
     4081 agccggaccc ttaatagtaa gcgcaagtaa aaccgtcatt ggtcacacag agcctgctgg
     4141 accgttggta ggcatgctct cagttctcaa ctcctttaaa gagggcgcag ttcccggctt
     4201 agctcacctg acagcagata acctcaatcc ggctttagac tgctcctccg tccctttact
     4261 tatcccctac cagcctgtcc acttggcagc tcctaagcca cacagggctg ctgtgatgag
     4321 ttatggcttc tccggcactc ttggaggtat agttcttgaa gcaccggatg aggaaaggct
     4381 tgaggaagaa ccacccaacg ataagcctat gttatttgtt gttagcgcca aaactcatac
     4441 tgcattgatt gagtaccttg gacgatacct tgaatttctg ctccaggcca atccccaaga
     4501 cttctgcgat atctgttaca ctagctgcgt tggacgtgag cattacaggt accgttttgc
     4561 atgtgtggca aacgatatgg aagatttaat tggccaactt cagaagaggc ttgggtctaa
     4621 ggttccacct aaaccgtcct ataaaagagg agccctcgct tttgctttca gtggacaagg
     4681 gacccagttt cgtggaatgg caacagaatt ggctaaggca tatagtggtt ttcgtaagat
     4741 agtcagtgat cttgctaaaa gagcatcaga actgtccggc catgcaatcg acaggtttct
     4801 gcttgcatat gacattgggg ctgagaatgt tgcaccggat agtgaagctg atcaaatctg
     4861 catattcgtt taccaatgta gtgttctcag atggctccaa acaatgggga ttcgtccgtc
     4921 agccgtgatc ggtcacagcc tcggtgagat tagcgcatcc gtagctgctg gagcattgtc
     4981 cttggactct gcccttgacc tcgtaataag tagagccagg ttactgaggt catctacgaa
     5041 tgccccagct ggcatggcag ctatgtctgc atcccaggat gaagtagtcg aactcatagg
     5101 aaagctcgat ttagataaag ctaatagcct cagcgtgtcc gtaataaacg gcccccagaa
     5161 caccgttgtt tcaggatctt ctgcagcaat agaaagtatc gttgctctcg caaagggacg
     5221 aaaaatcaag gccagcgcac tcaacattaa ccaggcattc catagtcctt acgtcgattc
     5281 tgcagtaccc ggcttacgag cttggagtga gaaacatata tcatcagctc gaccattgca
     5341 aatccctttg tactctaccc tcttgggcgc tcaagtttct gagggtcaaa tgttaaatcc
     5401 cgatcactgg gtagaccatg ctaggaaacc cgttcagttc gcacaagcag ccactataat
     5461 gaaggaatct ttcaccggag taataatcga tattggacct caagttgtag catggtccct
     5521 gctcttaagc aatggtctta cttccgtgac cgcactcgcc gcaaagcgtg gccgttcaca
     5581 gcaagtggcc ttcctctccg ccttagctga tctttatcag gactacggcg tcgtaccaga
     5641 ctttgttgga ctgtacgcac aacaggagga cgcttccaga ctgaaaaaga ccgatatctt
     5701 aacttacccc tttcagaggg ttcgacgtta cccttccttt ataccctcta gaagggcacc
     5761 cacccacgct catgtccaag acgaggaaac tctgtcatca ggatcatcaa ctccaacctt
     5821 agaaaatact gacctggaca gtgggaagga atctctgatg ggaccaacaa gagggctttt
     5881 gcgagtcgat gatcttaggg actcaatagt cagttcagtc aaagatgtcc tggaattgaa
     5941 gagtaacgaa gatctggacc tcagcgagtc ccttaatgct cttggcatgg actctataat
     6001 gttcgctcaa ttacgaaaaa ggatagggga gggcctcggc ctgtccgttc caatggtttt
     6061 tctcagcgat gccttcagta ttggagaaat ggtcagtaac ctcgtggaac aggccgaagc
     6121 ttccgaggac aattgactcg agggggggcc cggtaccctg ctttaatgag atatgcgaga
     6181 cgcctatgat cgcatgatat ttgctttcaa ttctgttgtg cacgttgtaa aaaacctgag
     6241 catgtgtagc tcagatcctt accgccggtt tcggttcatt ctaatgaata tatcacccgt
     6301 tactatcgta tttttatgaa taatattctc cgttcaattt actgattgta ccctactact
     6361 tatatgtaca atattaaaat gaaaacaata tattgtgctg aataggttta tagcgacatc
     6421 tatgatagag cgccacaata acaaacaatt gcgttttatt attacaaatc caattttaaa
     6481 aaaagcggca gaaccggtca aacctaaaag actgattaca taaatcttat tcaaatttca
     6541 aaagtgcccc aggggctagt atctacgaca caccgagcgg cgaactaata acgctcactg
     6601 aagggaactc cggttccccg ccggcgcgca tgggtgagat tccttgaagt tgagtattgg
     6661 ccgtccgctc taccgaaagt tacgggcacc attcaacccg gtccagcacg gcggccgggt
     6721 aaccgacttg ctgccccgag aattatgcag catttttttg gtgtatgtgg gccccaaatg
     6781 aagtgcaggt caaaccttga cagtgacgac aaatcgttgg gcgggtccag ggcgaatttt
     6841 gcgacaacat gtcgaggctc agcagcacta gccttgacag gatatattgg cgggtaaact
     6901 aagtcgctgt atgtgtttgt ttgagatctc atgtgagcaa aaggccagca aaaggccagg
     6961 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     7021 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     7081 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     7141 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     7201 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     7261 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     7321 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     7381 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
     7441 ggtatctgcg ctctgctgaa gccagttacc ttcggaagaa gagttggtag ctcttgatcc
     7501 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     7561 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     7621 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     7681 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatgt gtaacattgg
     7741 tctagtgatt agaaaaactc atcgagcatc aaatgaaact gcaatttatt catatcagga
     7801 ttatcaatac catatttttg aaaaagccgt ttctgtaatg aaggagaaaa ctcaccgagg
     7861 cagttccata ggatggcaag atcctggtat cggtctgcga ttccgactcg tccaacatca
     7921 atacaaccta ttaatttccc ctcgtcaaaa ataaggttat caagtgagaa atcaccatga
     7981 gtgacgactg aatccggtga gaatggcaaa agtttatgca tttctttcca gacttgttca
     8041 acaggccagc cattacgctc gtcatcaaaa tcactcgcat caaccaaacc gttattcatt
     8101 cgtgattgcg cctgagcgag acgaaatacg cgatcgctgt taaaaggaca attacaaaca
     8161 ggaatcgaat gcaaccggcg caggaacact gccagcgcat caacaatatt ttcacctgaa
     8221 tcaggatatt cttctaatac ctggaatgct gttttccctg ggatcgcagt ggtgagtaac
     8281 catgcatcat caggagtacg gataaaatgc ttgatggtcg gaagaggcat aaattccgtc
     8341 agccagttta gtctgaccat ctcatctgta acaacattgg caacgctacc tttgccatgt
     8401 ttcagaaaca actctggcgc atcgggcttc ccatacaatc ggtagattgt cgcacctgat
     8461 tgcccgacat tatcgcgagc ccatttatac ccatataaat cagcatccat gttggaattt
     8521 aatcgcggcc ttgagcaaga cgtttcccgt tgaatatggc tcataacacc ccttgtatta
     8581 ctgtttatgt aagcagacag ttttattgtt catgatgata tatttttatc ttgtgcaatg
     8641 taacatcaga gattttgaga cacaacgtgg ctttgttgaa taaatcgaac ttttgctgag
     8701 ttgaaggatc agatcacgca tcttcccgac aacgcagacc gttccgtggc aaagcaaaag
     8761 ttcaaaatca ccaactggtc cacctacaac aaagctctca tcaaccgtgg ctccctcact
     8821 ttctggctgg atgatggggc gattcaggcg atccccatcc aacagcccgc cgtcgagcgg
     8881 gct
//
