LOCUS       V013694                 5055 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V013694
VERSION     V013694
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5055)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5055
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    542..564
                     /label="LB T-DNA repeat"
                     /note="left border repeat from nopaline C58 T-DNA
                     (truncated)"
     promoter        638..982
                     /label="CaMV 35S promoter"
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     1016..1032
                     /label="SK primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             1040..2305
                     /gene="h3h"
                     /label="Hispidin-3-hydroxylase"
                     /note="Hispidin-3-hydroxylase from Neonothopanus nambi.
                     Accession#: A0A3G9K5C8"
     terminator      2330..3037
                     /label="OCS terminator"
                     /note="octopine synthase terminator"
     misc_feature    3047..3071
                     /label="RB T-DNA repeat"
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      complement(3162..3750)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(3924..4736)
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
     rep_origin      5027..5055
                     /label="pSa ori"
                     /note="origin of replication from bacterial plasmid pSa"
ORIGIN
        1 tttttatccc cggaagcctg tggatagagg gtagttatcc acgtgaaacc gctaatgccc
       61 cgcaaagcct tgattcacgg ggctttccgg cccgctccaa aaactatcca cgtgaaatcg
      121 ctaatcaggg tacgtgaaat cgctaatcgg agtacgtgaa atcgctaata aggtcacgtg
      181 aaatcgctaa tcaaaaaggc acgtgagaac gctaatagcc ctttcagatc aacagcttgc
      241 aaacacccct cgctccggca agtagttaca gcaagtagta tgttcaatta gcttttcaat
      301 tatgaatata tatatcaatt attggtcgcc cttggcttgt ggacaatgcg ctacgcgcac
      361 cggctccgcc cgtggacaac cgcaagcggt tgcccaccgt cgagcgccag cgcctttgcc
      421 cacaacccgg cggccggccg caacagatcg ttttataaat tttttttttt gaaaaagaaa
      481 aagcccgaaa ggcggcaacc tctcgggctt ctggatttcc gatccccgga attagagatc
      541 ttggcaggat atattgtggt gtaacgttat cagcttggta cgtacccccc tactccaaaa
      601 atgtcaaaga tacagtctca gaagaccaaa gggctattga gacttttcaa caaagggtaa
      661 tttcgggaaa cctcctcgga ttccattgcc cagctatctg tcacttcatc gaaaggacag
      721 tagaaaagga aggtggctcc tacaaatgcc atcattgcga taaaggaaag gctatcattc
      781 aagatgcctc tgccgacagt ggtcccaaag atggaccccc acccacgagg agcatcgtgg
      841 aaaaagaaga cgttccaacc acgtcttcaa agcaagtgga ttgatgtgac atctccactg
      901 acgtaaggga tgacgcacaa tcccactatc cttcgcaaga cccttcctct atataaggaa
      961 gtcatttcat ttggagagga cagcccaagc tgagctccac cgcggtggcg gccgctctag
     1021 aactagtgga tccgccacca tggcatcctt tgaaaattca ctcagtgtct taatagttgg
     1081 ggctggctta ggtggtttgg ccgctgctat agcactgcga aggcaaggac atgtagtcaa
     1141 gatatacgat agttcttcat tcaaggccga attgggagca ggattagctg taccacccaa
     1201 tactctccgt tccctgcaac aactgggctg taatacagaa aacctgaacg gagtagacaa
     1261 cctctgcttt acggccatgg gatacgacgg gagtgtaggt atgatgaata atatgaccga
     1321 ctaccgtgag gcatacggca cttcctggat aatggtacat cgagtggatc tccacaacga
     1381 gctgatgcga gtcgccttgg atccgggcgg gttagggcct cctgccactc ttcaccttaa
     1441 ccatcgtgtt acgttctgcg atgtggatgc ctgtacagtc acgtttacga acgggacgac
     1501 acagtctgct gacttgattg tcggagctga tggtattagg tctacgattc gaagattcgt
     1561 tctggaggag gacgtgactg ttccagctag cggaattgtc ggctttcgat ggttggtcca
     1621 ggccgatgct ctcgacccat atcccgaact ggattggatt gtcaaaaaac cacccttggg
     1681 agccaggttg atttctactc cacagaaccc gcagagtggc gtaggactgg ccgatagacg
     1741 aacgatcatt atttacgcat gcagaggtgg gaccatggtt aatgtattag ccgtacacga
     1801 cgacgaaaga gatcaaaaca ctgctgattg gtccgttccc gcatcaaagg atgatttatt
     1861 cagagtgttt cacgattacc atccaagatt ccgacgactc ttagagttag cacaggacat
     1921 taatttatgg cagatgaggg tggtcccggt cttgaaaaaa tgggtgaaca agagggtatg
     1981 cttgctgggt gatgccgccc atgcttcact gcccacactt ggacaaggct tcgggatggg
     2041 gttggaggat gcagtcgctt tgggcacgtt gttgcctaag ggaacgaccg catcacagat
     2101 cgagacaagg ctggccgttt acgaacagct gcgaaaggat cgagctgagt ttgtcgccgc
     2161 agagagctat gaggagcaat atgtcccaga gatgagaggg ctgtacctta ggtccaaaga
     2221 attgagagac agagtgatgg gttatgatat taaggtagag tcagagaagg ttttggaaac
     2281 actgttgcga tcatccaact cagcttgact cgaggggggg cccggtaccc tgctttaatg
     2341 agatatgcga gacgcctatg atcgcatgat atttgctttc aattctgttg tgcacgttgt
     2401 aaaaaacctg agcatgtgta gctcagatcc ttaccgccgg tttcggttca ttctaatgaa
     2461 tatatcaccc gttactatcg tatttttatg aataatattc tccgttcaat ttactgattg
     2521 taccctacta cttatatgta caatattaaa atgaaaacaa tatattgtgc tgaataggtt
     2581 tatagcgaca tctatgatag agcgccacaa taacaaacaa ttgcgtttta ttattacaaa
     2641 tccaatttta aaaaaagcgg cagaaccggt caaacctaaa agactgatta cataaatctt
     2701 attcaaattt caaaagtgcc ccaggggcta gtatctacga cacaccgagc ggcgaactaa
     2761 taacgctcac tgaagggaac tccggttccc cgccggcgcg catgggtgag attccttgaa
     2821 gttgagtatt ggccgtccgc tctaccgaaa gttacgggca ccattcaacc cggtccagca
     2881 cggcggccgg gtaaccgact tgctgccccg agaattatgc agcatttttt tggtgtatgt
     2941 gggccccaaa tgaagtgcag gtcaaacctt gacagtgacg acaaatcgtt gggcgggtcc
     3001 agggcgaatt ttgcgacaac atgtcgaggc tcagcagcac tagccttgac aggatatatt
     3061 ggcgggtaaa ctaagtcgct gtatgtgttt gtttgagatc tcatgtgagc aaaaggccag
     3121 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     3181 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     3241 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     3301 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     3361 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     3421 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     3481 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     3541 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     3601 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaag aagagttggt
     3661 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     3721 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     3781 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     3841 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     3901 gtgtaacatt ggtctagtga ttagaaaaac tcatcgagca tcaaatgaaa ctgcaattta
     3961 ttcatatcag gattatcaat accatatttt tgaaaaagcc gtttctgtaa tgaaggagaa
     4021 aactcaccga ggcagttcca taggatggca agatcctggt atcggtctgc gattccgact
     4081 cgtccaacat caatacaacc tattaatttc ccctcgtcaa aaataaggtt atcaagtgag
     4141 aaatcaccat gagtgacgac tgaatccggt gagaatggca aaagtttatg catttctttc
     4201 cagacttgtt caacaggcca gccattacgc tcgtcatcaa aatcactcgc atcaaccaaa
     4261 ccgttattca ttcgtgattg cgcctgagcg agacgaaata cgcgatcgct gttaaaagga
     4321 caattacaaa caggaatcga atgcaaccgg cgcaggaaca ctgccagcgc atcaacaata
     4381 ttttcacctg aatcaggata ttcttctaat acctggaatg ctgttttccc tgggatcgca
     4441 gtggtgagta accatgcatc atcaggagta cggataaaat gcttgatggt cggaagaggc
     4501 ataaattccg tcagccagtt tagtctgacc atctcatctg taacaacatt ggcaacgcta
     4561 cctttgccat gtttcagaaa caactctggc gcatcgggct tcccatacaa tcggtagatt
     4621 gtcgcacctg attgcccgac attatcgcga gcccatttat acccatataa atcagcatcc
     4681 atgttggaat ttaatcgcgg ccttgagcaa gacgtttccc gttgaatatg gctcataaca
     4741 ccccttgtat tactgtttat gtaagcagac agttttattg ttcatgatga tatattttta
     4801 tcttgtgcaa tgtaacatca gagattttga gacacaacgt ggctttgttg aataaatcga
     4861 acttttgctg agttgaagga tcagatcacg catcttcccg acaacgcaga ccgttccgtg
     4921 gcaaagcaaa agttcaaaat caccaactgg tccacctaca acaaagctct catcaaccgt
     4981 ggctccctca ctttctggct ggatgatggg gcgattcagg cgatccccat ccaacagccc
     5041 gccgtcgagc gggct
//
