LOCUS       V013690                 4656 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V013690
VERSION     V013690
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4656)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4656
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    542..564
                     /label="LB T-DNA repeat"
                     /note="left border repeat from nopaline C58 T-DNA
                     (truncated)"
     promoter        638..982
                     /label="CaMV 35S promoter"
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     1016..1032
                     /label="SK primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             1040..1906
                     /gene="cph"
                     /label="Caffeoylpyruvate hydrolase"
                     /note="Caffeoylpyruvate hydrolase from Neonothopanus nambi.
                     Accession#: A0A3G9JYJ6"
     terminator      1931..2638
                     /label="OCS terminator"
                     /note="octopine synthase terminator"
     misc_feature    2648..2672
                     /label="RB T-DNA repeat"
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      complement(2763..3351)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(3525..4337)
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
     rep_origin      4628..4656
                     /label="pSa ori"
                     /note="origin of replication from bacterial plasmid pSa"
ORIGIN
        1 tttttatccc cggaagcctg tggatagagg gtagttatcc acgtgaaacc gctaatgccc
       61 cgcaaagcct tgattcacgg ggctttccgg cccgctccaa aaactatcca cgtgaaatcg
      121 ctaatcaggg tacgtgaaat cgctaatcgg agtacgtgaa atcgctaata aggtcacgtg
      181 aaatcgctaa tcaaaaaggc acgtgagaac gctaatagcc ctttcagatc aacagcttgc
      241 aaacacccct cgctccggca agtagttaca gcaagtagta tgttcaatta gcttttcaat
      301 tatgaatata tatatcaatt attggtcgcc cttggcttgt ggacaatgcg ctacgcgcac
      361 cggctccgcc cgtggacaac cgcaagcggt tgcccaccgt cgagcgccag cgcctttgcc
      421 cacaacccgg cggccggccg caacagatcg ttttataaat tttttttttt gaaaaagaaa
      481 aagcccgaaa ggcggcaacc tctcgggctt ctggatttcc gatccccgga attagagatc
      541 ttggcaggat atattgtggt gtaacgttat cagcttggta cgtacccccc tactccaaaa
      601 atgtcaaaga tacagtctca gaagaccaaa gggctattga gacttttcaa caaagggtaa
      661 tttcgggaaa cctcctcgga ttccattgcc cagctatctg tcacttcatc gaaaggacag
      721 tagaaaagga aggtggctcc tacaaatgcc atcattgcga taaaggaaag gctatcattc
      781 aagatgcctc tgccgacagt ggtcccaaag atggaccccc acccacgagg agcatcgtgg
      841 aaaaagaaga cgttccaacc acgtcttcaa agcaagtgga ttgatgtgac atctccactg
      901 acgtaaggga tgacgcacaa tcccactatc cttcgcaaga cccttcctct atataaggaa
      961 gtcatttcat ttggagagga cagcccaagc tgagctccac cgcggtggcg gccgctctag
     1021 aactagtgga tccgccacca tggctcctat ctcctccacc tggtccagat taatcaggtt
     1081 cgtagctata gaaacgtcac tcgtgcatat aggcgaaccg attgatgcaa ctatggacgt
     1141 agggcttgct cgtcgagaag ggaaaactat tcaggcctat gagattatcg gatctggcag
     1201 cgccctggac ctttctgcac aggtgagtaa gaacgtgctc acagtacgag aattgttgat
     1261 gccactttct agagaagaga taaagacggt cagatgctta ggccttaact atcctgtcca
     1321 cgcaacagaa gccaatgttg ctgtaccgaa gtttccgaac ctgttctaca aaccagtcac
     1381 aagtctcatc gggcctgatg gtcttatcac gatccctagt gtagtgcaac cgcctaagga
     1441 acatcagtca gattatgaag ctgaacttgt gattgtaatc ggaaaggccg ccaagaacgt
     1501 gtctgaggac gaagccttgg attatgtctt aggctataca gctgctaacg atattagttt
     1561 ccgaaaacac cagctcgcag tctcacagtg gtcattctca aaaggatttg gatctctcct
     1621 tctcactata cgtatggccc agacacatag tggaaacata aataggttct cacgtgatca
     1681 aatctttaat gttaagaaaa caatcagctt tttgtctcaa ggaactaccc tcgaacctgg
     1741 gtctatcatt ttgactggca cccctgatgg ggtcggcttc gtcaggaacc cgcccttata
     1801 cttaaaagat ggggatgaag tgatgacttg gataggttcc ggcattggta cgcttgctaa
     1861 caccgttcaa gaagagaaaa catgctttgc aagcggtggg catgagtagc tcgagggggg
     1921 gcccggtacc ctgctttaat gagatatgcg agacgcctat gatcgcatga tatttgcttt
     1981 caattctgtt gtgcacgttg taaaaaacct gagcatgtgt agctcagatc cttaccgccg
     2041 gtttcggttc attctaatga atatatcacc cgttactatc gtatttttat gaataatatt
     2101 ctccgttcaa tttactgatt gtaccctact acttatatgt acaatattaa aatgaaaaca
     2161 atatattgtg ctgaataggt ttatagcgac atctatgata gagcgccaca ataacaaaca
     2221 attgcgtttt attattacaa atccaatttt aaaaaaagcg gcagaaccgg tcaaacctaa
     2281 aagactgatt acataaatct tattcaaatt tcaaaagtgc cccaggggct agtatctacg
     2341 acacaccgag cggcgaacta ataacgctca ctgaagggaa ctccggttcc ccgccggcgc
     2401 gcatgggtga gattccttga agttgagtat tggccgtccg ctctaccgaa agttacgggc
     2461 accattcaac ccggtccagc acggcggccg ggtaaccgac ttgctgcccc gagaattatg
     2521 cagcattttt ttggtgtatg tgggccccaa atgaagtgca ggtcaaacct tgacagtgac
     2581 gacaaatcgt tgggcgggtc cagggcgaat tttgcgacaa catgtcgagg ctcagcagca
     2641 ctagccttga caggatatat tggcgggtaa actaagtcgc tgtatgtgtt tgtttgagat
     2701 ctcatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     2761 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     2821 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     2881 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     2941 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     3001 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     3061 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     3121 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     3181 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
     3241 accttcggaa gaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     3301 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     3361 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     3421 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     3481 aaatcaatct aaagtatata tgtgtaacat tggtctagtg attagaaaaa ctcatcgagc
     3541 atcaaatgaa actgcaattt attcatatca ggattatcaa taccatattt ttgaaaaagc
     3601 cgtttctgta atgaaggaga aaactcaccg aggcagttcc ataggatggc aagatcctgg
     3661 tatcggtctg cgattccgac tcgtccaaca tcaatacaac ctattaattt cccctcgtca
     3721 aaaataaggt tatcaagtga gaaatcacca tgagtgacga ctgaatccgg tgagaatggc
     3781 aaaagtttat gcatttcttt ccagacttgt tcaacaggcc agccattacg ctcgtcatca
     3841 aaatcactcg catcaaccaa accgttattc attcgtgatt gcgcctgagc gagacgaaat
     3901 acgcgatcgc tgttaaaagg acaattacaa acaggaatcg aatgcaaccg gcgcaggaac
     3961 actgccagcg catcaacaat attttcacct gaatcaggat attcttctaa tacctggaat
     4021 gctgttttcc ctgggatcgc agtggtgagt aaccatgcat catcaggagt acggataaaa
     4081 tgcttgatgg tcggaagagg cataaattcc gtcagccagt ttagtctgac catctcatct
     4141 gtaacaacat tggcaacgct acctttgcca tgtttcagaa acaactctgg cgcatcgggc
     4201 ttcccataca atcggtagat tgtcgcacct gattgcccga cattatcgcg agcccattta
     4261 tacccatata aatcagcatc catgttggaa tttaatcgcg gccttgagca agacgtttcc
     4321 cgttgaatat ggctcataac accccttgta ttactgttta tgtaagcaga cagttttatt
     4381 gttcatgatg atatattttt atcttgtgca atgtaacatc agagattttg agacacaacg
     4441 tggctttgtt gaataaatcg aacttttgct gagttgaagg atcagatcac gcatcttccc
     4501 gacaacgcag accgttccgt ggcaaagcaa aagttcaaaa tcaccaactg gtccacctac
     4561 aacaaagctc tcatcaaccg tggctccctc actttctggc tggatgatgg ggcgattcag
     4621 gcgatcccca tccaacagcc cgccgtcgag cgggct
//
