LOCUS       V013695                 4827 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V013695
VERSION     V013695
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4827)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4827
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    542..564
                     /label="LB T-DNA repeat"
                     /note="left border repeat from nopaline C58 T-DNA
                     (truncated)"
     promoter        638..982
                     /label="CaMV 35S promoter"
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     1016..1032
                     /label="SK primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             1040..2071
                     /gene="npgA"
                     /label="4'-phosphopantetheinyl transferase NpgA"
                     /note="4'-phosphopantetheinyl transferase NpgA from
                     Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS
                     112.46 / NRRL 194 / M139). Accession#: G5EB87"
     terminator      2102..2809
                     /label="OCS terminator"
                     /note="octopine synthase terminator"
     misc_feature    2819..2843
                     /label="RB T-DNA repeat"
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      complement(2934..3522)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(3696..4508)
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
     rep_origin      4799..4827
                     /label="pSa ori"
                     /note="origin of replication from bacterial plasmid pSa"
ORIGIN
        1 tttttatccc cggaagcctg tggatagagg gtagttatcc acgtgaaacc gctaatgccc
       61 cgcaaagcct tgattcacgg ggctttccgg cccgctccaa aaactatcca cgtgaaatcg
      121 ctaatcaggg tacgtgaaat cgctaatcgg agtacgtgaa atcgctaata aggtcacgtg
      181 aaatcgctaa tcaaaaaggc acgtgagaac gctaatagcc ctttcagatc aacagcttgc
      241 aaacacccct cgctccggca agtagttaca gcaagtagta tgttcaatta gcttttcaat
      301 tatgaatata tatatcaatt attggtcgcc cttggcttgt ggacaatgcg ctacgcgcac
      361 cggctccgcc cgtggacaac cgcaagcggt tgcccaccgt cgagcgccag cgcctttgcc
      421 cacaacccgg cggccggccg caacagatcg ttttataaat tttttttttt gaaaaagaaa
      481 aagcccgaaa ggcggcaacc tctcgggctt ctggatttcc gatccccgga attagagatc
      541 ttggcaggat atattgtggt gtaacgttat cagcttggta cgtacccccc tactccaaaa
      601 atgtcaaaga tacagtctca gaagaccaaa gggctattga gacttttcaa caaagggtaa
      661 tttcgggaaa cctcctcgga ttccattgcc cagctatctg tcacttcatc gaaaggacag
      721 tagaaaagga aggtggctcc tacaaatgcc atcattgcga taaaggaaag gctatcattc
      781 aagatgcctc tgccgacagt ggtcccaaag atggaccccc acccacgagg agcatcgtgg
      841 aaaaagaaga cgttccaacc acgtcttcaa agcaagtgga ttgatgtgac atctccactg
      901 acgtaaggga tgacgcacaa tcccactatc cttcgcaaga cccttcctct atataaggaa
      961 gtcatttcat ttggagagga cagcccaagc tgagctccac cgcggtggcg gccgctctag
     1021 aactagtgga tccgccacca tggtgcagga cacttcaagt gctagcacaa gtccaatttt
     1081 aaccagatgg tatatagaca ctcgacctct cacggcaagt accgccgcac ttccgctgct
     1141 ggaaacgctg caaccagctg atcagatctc cgtccaaaaa tattatcatc tcaaggacaa
     1201 gcacatgtca cttgcctcca accttttgaa gtatttattc gttcatcgaa attgccgaat
     1261 accatggagt agtattgtca tatcaagaac gcccgacccg cacagaaggc cttgctatat
     1321 acctccttca ggttcacaag aggactcctt caaagacgga tatacgggaa tcaacgtaga
     1381 attcaacgtt agtcaccaag ccagcatggt ggcaatcgca ggtacggctt tcacacctaa
     1441 tagcggtgga gatagcaaac tcaaaccgga ggtcggaata gatataacat gtgtaaatga
     1501 gaggcagggc aggaatggag aagaaagatc cttggaatcc ctgaggcagt atatagatat
     1561 attcagcgaa gtattcagca ccgcagagat ggctaatata aggcgacttg atggggtttc
     1621 ctcaagttcc cttagtgcag acagactcgt agattatgga taccgattat tctacaccta
     1681 ttgggcctta aaagaggctt atatcaagat gacaggtgag gccttattgg ctccttggtt
     1741 gagagagctg gagttttcca acgtggtcgc tccagccgct gtcgccgaaa gcggggactc
     1801 tgctggagac tttggcgaac cgtacactgg cgtgcgtaca acattgtata agaacctcgt
     1861 agaggacgta cgaattgagg tagctgcttt gggaggagat tatctgttcg caacagccgc
     1921 tagaggtggt ggtataggcg catcctcacg tcctggtggt ggcccagatg gctctggaat
     1981 tagatcccag gacccttgga ggccgttcaa gaaactcgat atagagcgtg acatacagcc
     2041 atgtgctacg ggcgtttgta attgcctgtc ctcaggatag ctcgaggggg ggcccggtac
     2101 cctgctttaa tgagatatgc gagacgccta tgatcgcatg atatttgctt tcaattctgt
     2161 tgtgcacgtt gtaaaaaacc tgagcatgtg tagctcagat ccttaccgcc ggtttcggtt
     2221 cattctaatg aatatatcac ccgttactat cgtattttta tgaataatat tctccgttca
     2281 atttactgat tgtaccctac tacttatatg tacaatatta aaatgaaaac aatatattgt
     2341 gctgaatagg tttatagcga catctatgat agagcgccac aataacaaac aattgcgttt
     2401 tattattaca aatccaattt taaaaaaagc ggcagaaccg gtcaaaccta aaagactgat
     2461 tacataaatc ttattcaaat ttcaaaagtg ccccaggggc tagtatctac gacacaccga
     2521 gcggcgaact aataacgctc actgaaggga actccggttc cccgccggcg cgcatgggtg
     2581 agattccttg aagttgagta ttggccgtcc gctctaccga aagttacggg caccattcaa
     2641 cccggtccag cacggcggcc gggtaaccga cttgctgccc cgagaattat gcagcatttt
     2701 tttggtgtat gtgggcccca aatgaagtgc aggtcaaacc ttgacagtga cgacaaatcg
     2761 ttgggcgggt ccagggcgaa ttttgcgaca acatgtcgag gctcagcagc actagccttg
     2821 acaggatata ttggcgggta aactaagtcg ctgtatgtgt ttgtttgaga tctcatgtga
     2881 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     2941 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     3001 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     3061 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     3121 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     3181 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     3241 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     3301 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     3361 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     3421 agaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     3481 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     3541 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     3601 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     3661 taaagtatat atgtgtaaca ttggtctagt gattagaaaa actcatcgag catcaaatga
     3721 aactgcaatt tattcatatc aggattatca ataccatatt tttgaaaaag ccgtttctgt
     3781 aatgaaggag aaaactcacc gaggcagttc cataggatgg caagatcctg gtatcggtct
     3841 gcgattccga ctcgtccaac atcaatacaa cctattaatt tcccctcgtc aaaaataagg
     3901 ttatcaagtg agaaatcacc atgagtgacg actgaatccg gtgagaatgg caaaagttta
     3961 tgcatttctt tccagacttg ttcaacaggc cagccattac gctcgtcatc aaaatcactc
     4021 gcatcaacca aaccgttatt cattcgtgat tgcgcctgag cgagacgaaa tacgcgatcg
     4081 ctgttaaaag gacaattaca aacaggaatc gaatgcaacc ggcgcaggaa cactgccagc
     4141 gcatcaacaa tattttcacc tgaatcagga tattcttcta atacctggaa tgctgttttc
     4201 cctgggatcg cagtggtgag taaccatgca tcatcaggag tacggataaa atgcttgatg
     4261 gtcggaagag gcataaattc cgtcagccag tttagtctga ccatctcatc tgtaacaaca
     4321 ttggcaacgc tacctttgcc atgtttcaga aacaactctg gcgcatcggg cttcccatac
     4381 aatcggtaga ttgtcgcacc tgattgcccg acattatcgc gagcccattt atacccatat
     4441 aaatcagcat ccatgttgga atttaatcgc ggccttgagc aagacgtttc ccgttgaata
     4501 tggctcataa caccccttgt attactgttt atgtaagcag acagttttat tgttcatgat
     4561 gatatatttt tatcttgtgc aatgtaacat cagagatttt gagacacaac gtggctttgt
     4621 tgaataaatc gaacttttgc tgagttgaag gatcagatca cgcatcttcc cgacaacgca
     4681 gaccgttccg tggcaaagca aaagttcaaa atcaccaact ggtccaccta caacaaagct
     4741 ctcatcaacc gtggctccct cactttctgg ctggatgatg gggcgattca ggcgatcccc
     4801 atccaacagc ccgccgtcga gcgggct
//
