LOCUS       pGreenII_0579.        5663 bp DNA     circular SYN 06-JAN-2024
DEFINITION  Agrobacterium binary vector with kanamycin- resistance and 
            luciferase genes.
ACCESSION   .
VERSION     .
KEYWORDS    pGreenII 0579
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5663)
  AUTHORS   pGreen website
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 5663)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5663
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      63..498
                     /label=pSa ori
                     /note="origin of replication from bacterial plasmid pSa"
     misc_feature    633..655
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA
                     (truncated)"
     polyA_signal    complement(663..839)
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(958..2607)
                     /label=luciferase
                     /note="firefly luciferase"
     promoter        complement(2618..2963)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     3185..3201
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        3208..3226
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    complement(3235..3341)
                     /label=MCS
                     /note="pBluescript multiple cloning site"
     promoter        complement(3354..3372)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3393..3409)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(3417..3433)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3441..3471)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3486..3507)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     misc_feature    3746..3770
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      complement(3861..4449)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4623..5435)
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
ORIGIN
        1 agctctcatc aaccgtggct ccctcacttt ctggctggat gatggggcga ttcaggcgat
       61 ccccatccaa cagcccgccg tcgagcgggc ttttttatcc ccggaagcct gtggatagag
      121 ggtagttatc cacgtgaaac cgctaatgcc ccgcaaagcc ttgattcacg gggctttccg
      181 gcccgctcca aaaactatcc acgtgaaatc gctaatcagg gtacgtgaaa tcgctaatcg
      241 gagtacgtga aatcgctaat aaggtcacgt gaaatcgcta atcaaaaagg cacgtgagaa
      301 cgctaatagc cctttcagat caacagcttg caaacacccc tcgctccggc aagtagttac
      361 agcaagtagt atgttcaatt agcttttcaa ttatgaatat atatatcaat tattggtcgc
      421 ccttggcttg tggacaatgc gctacgcgca ccggctccgc ccgtggacaa ccgcaagcgg
      481 ttgcccaccg tcgagcgcca gcgcctttgc ccacaacccg gcggccggcc gcaacagatc
      541 gttttataaa tttttttttt tgaaaaagaa aaagcccgaa aggcggcaac ctctcgggct
      601 tctggatttc cgatccccgg aattagagat cttggcagga tatattgtgg tgtaacgtta
      661 tcgatctgga ttttagtact ggattttggt tttaggaatt agaaatttta ttgatagaag
      721 tattttacaa atacaaatac atactaaggg tttcttatat gctcaacaca tgagcgaaac
      781 cctataagaa ccctaatttc ccttatcggg aaactactca cacattattt atggagaaaa
      841 tagagagaga tagatttgta gagagagact ggtgatttca gcgtaccgaa ttaattctct
      901 agctagagta ttacaatagc taagaatttc gtcatcgctg aatacagtta cattttacaa
      961 tttggacttt ccgcccttct tggcctttat gaggatctct ctgatttttc ttgcgtcgag
     1021 ttttccggta agacctttcg gtacttcgtc cacaaacaca actcctccgc gcaacttttt
     1081 ggcggttgtt acttgactgg cgacgtaatc cacgatctct ttttccgtca tcgtctttcc
     1141 gtgctccaaa acaacaacgg cggcgggaag ttcaccggcg tcatcgtcgg gaagacctgc
     1201 cacgcccgcg tcgaagatgt tggggtgttg taacaatatc gactccaatt cagcgggggc
     1261 cacctggtat cctttgtatt taattaaaga cttcaagcgg tcaactatga agaagtgttc
     1321 gtcttcgtcc cagtaagcta tgtctccaga atgtagccat ccatccttgt caatcaaggc
     1381 gttggtcgct tccggattgt ttacataacc ggacataatc ataggtcctc tgacacataa
     1441 ttcgcctctc tgattaacgc ccagcgtttt cccggtatcc agatccacaa ccttcgcttc
     1501 aaaaaatgga acaactttac cgaccgcgcc cggtttatca tccccctcgg gtgtaatcag
     1561 aatagctgat gtagtctcag tgagcccata tccttgtcgt atccctggaa gatggaagcg
     1621 ttttgcaacc gcttccccga cttctttcga aagaggtgcg cccccagaag caatttcgtg
     1681 taaattagat aaatcgtatt tgtcaatcag agtgcttttg gcgaagaatg aaaatagggt
     1741 tggtactagc aacgcacttt gaattttgta atcctgaagg gatcgtaaaa acagctcttc
     1801 ttcaaatcta tacattaaga cgactcgaaa tccacatatc aaatatccga gtgtagtaaa
     1861 cattccaaaa ccgtgatgga atggaacaac acttaaaatc gcagtatccg gaatgatttg
     1921 attgccaaaa ataggatctc tggcatgcga gaatctgacg caggcagttc tatgcggaag
     1981 ggccacaccc ttaggtaacc cagtagatcc agaggagttc attatcagtg caattgtttt
     2041 gtcacgatca aaggactctg gtacaaaatc gtattcatta aaaccgggag gtagatgaga
     2101 tgtgacgaac gtgtacatcg actgaaatcc ctggtaatcc gttttagaat ccatgataat
     2161 aattttctgg attattggta attttttttg cacgttcaaa attttttgca accccttttt
     2221 ggaaacaaac actacggtag gctgcgaaat gttcatactg ttgagcaatt cacgttcatt
     2281 ataaatgtcg ttcgcgggcg caactgcaac tccgataaat aacgcgccca acaccggcat
     2341 aaagaattga agagagtttt cactgcatac gacgattctg tgatttgtat tcagcccata
     2401 tcgtttcata gcttctgcca accgaacgga catttcgaag tattccgcgt acgtgatgtt
     2461 cacctcgata tgtgcatctg taaaagcaat tgttccagga accagggcgt atctcttcat
     2521 agccttatgc agttgctctc cagcggttcc atcctctagc ggatagaatg gcgccgggcc
     2581 tttctttatg tttttggcgt cttccatgag cttgggctgt cctctccaaa tgaaatgaac
     2641 ttccttatat agaggaaggg tcttgcgaag gatagtggga ttgtgcgtca tcccttacgt
     2701 cagtggagat gtcacatcaa tccacttgct ttgaagacgt ggttggaacg tcttcttttt
     2761 ccacgatgct cctcgtgggt gggggtccat ctttgggacc actgtcggca gaggcatctt
     2821 gaatgatagc ctttccttta tcgcaatgat ggcatttgta ggagccacct tccttttcta
     2881 ctgtcctttc gatgaagtga cagatagctg ggcaatggaa tccgaggagg tttcccgaaa
     2941 ttaccctttg ttgaaaagtc tcaatagccc tttggtcttc tgagactgta tctttgacat
     3001 ttttggagta ggggtacgat aacattaacg tttacaattt cgcgccattc gccattcagg
     3061 ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg
     3121 aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga
     3181 cgttgtaaaa cgacggccag tgaattgtaa tacgactcac tatagggcga attgggtacc
     3241 gggccccccc tcgaggtcga cggtatcgat aagcttgata tcgattcctg cagcccgggg
     3301 gatccactag ttctagagcg gccgccaccg cggtggagct ccagcttttg ttccctttag
     3361 tgagggttaa ttccgagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
     3421 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
     3481 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
     3541 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
     3601 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     3661 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     3721 aacgcaggaa agaacatgaa ggccttgaca ggatatattg gcgggtaaac taagtcgctg
     3781 tatgtgtttg tttgagatct catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     3841 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     3901 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     3961 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     4021 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     4081 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     4141 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     4201 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     4261 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
     4321 gctctgctga agccagttac cttcggaaga agagttggta gctcttgatc cggcaaacaa
     4381 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     4441 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     4501 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     4561 aattaaaaat gaagttttaa atcaatctaa agtatatatg tgtaacattg gtctagtgat
     4621 tagaaaaact catcgagcat caaatgaaac tgcaatttat tcatatcagg attatcaata
     4681 ccatattttt gaaaaagccg tttctgtaat gaaggagaaa actcaccgag gcagttccat
     4741 aggatggcaa gatcctggta tcggtctgcg attccgactc gtccaacatc aatacaacct
     4801 attaatttcc cctcgtcaaa aataaggtta tcaagtgaga aatcaccatg agtgacgact
     4861 gaatccggtg agaatggcaa aagtttatgc atttctttcc agacttgttc aacaggccag
     4921 ccattacgct cgtcatcaaa atcactcgca tcaaccaaac cgttattcat tcgtgattgc
     4981 gcctgagcga gacgaaatac gcgatcgctg ttaaaaggac aattacaaac aggaatcgaa
     5041 tgcaaccggc gcaggaacac tgccagcgca tcaacaatat tttcacctga atcaggatat
     5101 tcttctaata cctggaatgc tgttttccct gggatcgcag tggtgagtaa ccatgcatca
     5161 tcaggagtac ggataaaatg cttgatggtc ggaagaggca taaattccgt cagccagttt
     5221 agtctgacca tctcatctgt aacaacattg gcaacgctac ctttgccatg tttcagaaac
     5281 aactctggcg catcgggctt cccatacaat cggtagattg tcgcacctga ttgcccgaca
     5341 ttatcgcgag cccatttata cccatataaa tcagcatcca tgttggaatt taatcgcggc
     5401 cttgagcaag acgtttcccg ttgaatatgg ctcataacac cccttgtatt actgtttatg
     5461 taagcagaca gttttattgt tcatgatgat atatttttat cttgtgcaat gtaacatcag
     5521 agattttgag acacaacgtg gctttgttga ataaatcgaa cttttgctga gttgaaggat
     5581 cagatcacgc atcttcccga caacgcagac cgttccgtgg caaagcaaaa gttcaaaatc
     5641 accaactggt ccacctacaa caa
//
