LOCUS       V015483                 6861 bp    DNA     circular SYN 28-OCT-2020
DEFINITION  Exported.
ACCESSION   V015483
VERSION     V015483
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6861)
  AUTHORS   Declercq M, Biquand E, Karim M, Pietrosemoli N, Jacob Y, Demeret C,
            Barbezange C, van der Werf S.
  TITLE     Influenza A virus co-opts ERI1 exonuclease bound to histone mRNA to
            promote viral transcription
  JOURNAL   Nucleic Acids Res 48 (18), 10428-10440 (2020)
   PUBMED   32960265
REFERENCE   2  (bases 1 to 6861)
  AUTHORS   Declercq M, Biquand E, Demeret C, Karim M, Pietrosemoli N, Jacob Y,
            Demeret C, Van der Werf S, Barbezange C.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2020) National Influenza Centre, Sciensano, Rue
            Juliette Wytsman 14, Brussels 1180, Belgium
REFERENCE   3  (bases 1 to 6861)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic
            Acids Res"; date: "2020"; volume: "48"; issue: "18"; pages:
            "10428-10440"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (03-SEP-2020) National Influenza Centre, Sciensano, Rue Juliette
            Wytsman 14, Brussels 1180, Belgium"
FEATURES             Location/Qualifiers
     source          1..6861
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        139..517
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        518..729
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     intron          890..1022
                     /label="chimeric intron"
                     /note="chimera between introns from human beta-globin and
                     immunoglobulin heavy chain genes"
     misc_feature    1045..1060
                     /label="sequencing primer"
                     /note="sequencing primer"
     promoter        1067..1085
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    1120..1389
                     /label="Gaussia luciferase domain 2"
                     /note="Gaussia luciferase domain 2"
     misc_feature    1278..1298
                     /label="sequencing primer"
                     /note="sequencing primer"
     protein_bind    1393..1417
                     /label="attB1"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1420..2466
                     /gene="ERI1"
                     /label="3'-5' exoribonuclease 1"
                     /note="3'-5' exoribonuclease 1 from Homo sapiens.
                     Accession#: Q8IV48"
     protein_bind    complement(2471..2495)
                     /label="attB2"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    complement(2547..2668)
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     misc_feature    complement(2785..2853)
                     /label="sequencing primer"
                     /note="sequencing primer"
     rep_origin      2854..3309
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        3428..3785
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             3836..4627
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    4694..4742
                     /label="poly(A) signal"
                     /note="synthetic polyadenylation signal"
     promoter        5064..5168
                     /label="AmpR promoter"
     CDS             5169..6026
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      6200..6788
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactg
      661 cgatcgcccg ccccgttgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata
      721 agcagagctc gtttagtgaa ccgtcagatc actagaagct ttattgcggt agtttatcac
      781 agttaaattg ctaacgcagt cagtgcttct gacacaacag tctcgaactt aagctgcagt
      841 gactctctta aggtagcctt gcagaagttg gtcgtgaggc actgggcagg taagtatcaa
      901 ggttacaaga caggtttaag gagaccaata gaaactgggc ttgtcgagac agagaagact
      961 cttgcgtttc tgataggcac ctattggtct tactgacatc cactttgcct ttctctccac
     1021 aggtgtccac tcccagttca attacagctc ttaaggctag agtacttaat acgactcact
     1081 ataggctagc ctcgagaatt gcggatcgcc gccaccatgg ggatctccga ggcgatcgtc
     1141 gacattcctg agattcctgg gttcaaggac ttggagccca tggagcagtt catcgcacag
     1201 gtcgatctgt gtgtggactg cacaactggc tgcctcaaag ggcttgccaa cgtgcagtgt
     1261 tctgacctgc tcaagaagtg gctgccgcaa cgctgtgcga cctttgccag caagatccag
     1321 ggccaggtgg acaagatcaa gggggccggt ggtgacggag gagggggctc cggaggaggg
     1381 ggctccccca tcacaagttt gtacaaaaaa gcaggcttca tggaggatcc acagagtaaa
     1441 gagcctgccg gcgaggccgt ggctctcgcg ctgctggagt cgccgcggcc ggagggcggg
     1501 gaggagccgc cgcgtcccag tcccgaggaa actcaacagt gtaaatttga tggccaggag
     1561 acaaaaggat ccaagttcat tacctccagt gcgagtgact tcagtgaccc ggtttacaaa
     1621 gagattgcca ttacgaatgg ctgtattaat agaatgagta aggaagaact cagagctaag
     1681 ctttcagaat tcaagcttga aactagagga gtaaaggatg ttctaaagaa gagactgaaa
     1741 aactattata agaagcagaa gctgatgctg aaagagagca attttgctga cagttattat
     1801 gactacattt gtattattgc ctttggagcc acttgtgaag aaggaaaccc acctgagttt
     1861 gtacatgaaa taattgaatt tccggttgtt ttactgaata cgcatacttt agaaatagaa
     1921 gacacgtttc agcagtatgt aagaccagag attaacacac agctgtctga tttctgcatc
     1981 agtctaactg gaattactca ggatcaggta gacagagctg ataccttccc tcaggtacta
     2041 aaaaaagtaa ttgactggat gaaattgaag gaattaggaa caaagtataa atactcactt
     2101 ttaacagatg gttcttggga tatgagtaag ttcttgaaca ttcagtgtca actcagcagg
     2161 ctcaaatacc ctccttttgc gaaaaagtgg atcaatattc ggaagtcata tggaaatttt
     2221 tacaaggttc ctagaagcca aaccaaactg acaataatgc ttgaaaaatt aggaatggat
     2281 tatgatgggc ggcctcactg tggtcttgat gactctaaga atatcgcccg aatagcagtt
     2341 cgaatgcttc aggatgggtg tgaactccga atcaacgaga aaatgcatgc aggacagcta
     2401 atgagtgtgt cctcttcctt accaatagag ggcactccac caccacaaat gccacatttt
     2461 agaaagtaag acccagcttt cttgtacaaa gtggtgatgg gcggccgctt ccctttagtg
     2521 agggttaatg cttcgagcag acatgataag atacattgat gagtttggac aaaccacaac
     2581 tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt
     2641 aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca
     2701 ggttcagggg gagatgtggg aggtttttta aagcaagtaa aacctctaca aatgtggtaa
     2761 aatccgataa ggatcgatcc gggctggcgt aatagcgaag aggcccgcac cgatcgccct
     2821 tcccaacagt tgcgcagcct gaatggcgaa tggacgcgcc ctgtagcggc gcattaagcg
     2881 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg
     2941 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
     3001 taaatcgggg gctcccttta gggttccgat ttagagcttt acggcacctc gaccgcaaaa
     3061 aacttgattt gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc
     3121 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac
     3181 tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt
     3241 ggttaaaaaa tgagctgatt taacaaatat ttaacgcgaa ttttaacaaa atattaacgt
     3301 ttacaatttc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc
     3361 atacgcggat ctgcgcagca ccatggcctg aaataacctc tgaaagagga acttggttag
     3421 gtaccttctg aggcggaaag aaccagctgt ggaatgtgtg tcagttaggg tgtggaaagt
     3481 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
     3541 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
     3601 agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt
     3661 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
     3721 cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt
     3781 gcaaaaagct tgattcttct gacacaacag tctcgaactt aaggctagag ccaccatgat
     3841 tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta
     3901 tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca
     3961 ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcagga
     4021 cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga
     4081 cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct
     4141 cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg
     4201 gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga
     4261 gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca
     4321 tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgcgcatgc ccgacggcga
     4381 ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg
     4441 cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc
     4501 gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt
     4561 gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga
     4621 gttcttctga gcgggactct ggggttcgaa atgaccgacc aagcgacgcc caacctgcca
     4681 tcacgatggc cgcaataaaa tatctttatt ttcattacat ctgtgtgttg gttttttgtg
     4741 tgaatcgata gcgataagga tctgccccgg cgccgatccg cgtatggtgc actctcagta
     4801 caatctgctc tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg
     4861 cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg
     4921 ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc
     4981 tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag
     5041 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     5101 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     5161 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
     5221 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
     5281 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
     5341 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
     5401 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
     5461 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
     5521 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
     5581 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
     5641 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
     5701 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
     5761 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
     5821 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
     5881 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
     5941 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
     6001 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     6061 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     6121 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     6181 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     6241 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     6301 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc
     6361 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     6421 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     6481 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     6541 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     6601 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     6661 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     6721 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     6781 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     6841 ctcacatggc tcgacagatc t
//
