LOCUS       62056_11800        6756 bp DNA     circular SYN 28-OCT-2020
DEFINITION  Cloning vector pGluc2-Nter-ERI1-deltaSAP, complete sequence.
ACCESSION   MT966991
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6756)
  AUTHORS   Declercq M, Biquand E, Karim M, Pietrosemoli N, Jacob Y, Demeret C, 
            Barbezange C, van der Werf S.
  TITLE     Influenza A virus co-opts ERI1 exonuclease bound to histone mRNA to 
            promote viral transcription
  JOURNAL   Nucleic Acids Res 48 (18), 10428-10440 (2020)
  PUBMED    32960265
REFERENCE   2  (bases 1 to 6756)
  AUTHORS   Declercq M, Biquand E, Demeret C, Karim M, Pietrosemoli N, Jacob Y, 
            Demeret C, Van der Werf S, Barbezange C.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2020) National Influenza Centre, Sciensano, Rue 
            Juliette Wytsman 14, Brussels 1180, Belgium
REFERENCE   3  (bases 1 to 6756)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res"; date: "2020"; volume: "48"; issue: "18"; pages: 
            "10428-10440"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (03-SEP-2020) National Influenza Centre, Sciensano, Rue Juliette 
            Wytsman 14, Brussels 1180, Belgium"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6756
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        139..517
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        518..729
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     intron          890..1022
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     misc_feature    1045..1060
                     /label=sequencing primer
                     /note="sequencing primer"
     promoter        1067..1085
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    1120..1389
                     /label=Gaussia luciferase domain 2
                     /note="Gaussia luciferase domain 2"
     misc_feature    1278..1298
                     /label=sequencing primer
                     /note="sequencing primer"
     protein_bind    1393..1417
                     /label=attB1
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    1420..2364
                     /note="exoribonuclease 1 with deleted SAP (SAF-box, Acinus
                     and PIAS) domain"
     protein_bind    complement(2366..2390)
                     /label=attB2
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    complement(2442..2563)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      2749..3204
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        3323..3680
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             3731..4522
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    4589..4637
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     promoter        4959..5063
                     /label=AmpR promoter
     CDS             5064..5921
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      6095..6683
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactg
      661 cgatcgcccg ccccgttgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata
      721 agcagagctc gtttagtgaa ccgtcagatc actagaagct ttattgcggt agtttatcac
      781 agttaaattg ctaacgcagt cagtgcttct gacacaacag tctcgaactt aagctgcagt
      841 gactctctta aggtagcctt gcagaagttg gtcgtgaggc actgggcagg taagtatcaa
      901 ggttacaaga caggtttaag gagaccaata gaaactgggc ttgtcgagac agagaagact
      961 cttgcgtttc tgataggcac ctattggtct tactgacatc cactttgcct ttctctccac
     1021 aggtgtccac tcccagttca attacagctc ttaaggctag agtacttaat acgactcact
     1081 ataggctagc ctcgagaatt gcggatcgcc gccaccatgg ggatctccga ggcgatcgtc
     1141 gacattcctg agattcctgg gttcaaggac ttggagccca tggagcagtt catcgcacag
     1201 gtcgatctgt gtgtggactg cacaactggc tgcctcaaag ggcttgccaa cgtgcagtgt
     1261 tctgacctgc tcaagaagtg gctgccgcaa cgctgtgcga cctttgccag caagatccag
     1321 ggccaggtgg acaagatcaa gggggccggt ggtgacggag gagggggctc cggaggaggg
     1381 ggctccccca tcacaagttt gtacaaaaaa gcaggcttca tggaggatcc acagagtaaa
     1441 gagcctgccg gcgaggccgt ggctctcgcg ctgctggagt cgccgcggcc ggagggcggg
     1501 gaggagccgc cgcgtcccag tcccgaggaa actcaacagt gtaaatttga tggccaggag
     1561 acaaaaggat ccaagttcat tacctccagt gcgagtgact tcagtgaccc ggtttacaaa
     1621 gagattgcca ttacgaatgg ctgtaagaag cagaagctga tgctgaaaga gagcaatttt
     1681 gctgacagtt attatgacta catttgtatt attgactttg aagccacttg tgaagaagga
     1741 aacccacctg agtttgtaca tgaaataatt gaatttccgg ttgttttact gaatacgcat
     1801 actttagaaa tagaagacac gtttcagcag tatgtaagac cagagattaa cacacagctg
     1861 tctgatttct gcatcagtct aactggaatt actcaggatc aggtagacag agctgatacc
     1921 ttccctcagg tactaaaaaa agtaattgac tggatgaaat tgaaggaatt aggaacaaag
     1981 tataaatact cacttttaac agatggttct tgggatatga gtaagttctt gaacattcag
     2041 tgtcaactca gcaggctcaa ataccctcct tttgcgaaaa agtggatcaa tattcggaag
     2101 tcatatggaa atttttacaa ggttcctaga agccaaacca aactgacaat aatgcttgaa
     2161 aaattaggaa tggattatga tgggcggcct cactgtggtc ttgatgactc taagaatatc
     2221 gcccgaatag cagttcgaat gcttcaggat gggtgtgaac tccgaatcaa cgagaaaatg
     2281 catgcaggac agctaatgag tgtgtcctct tccttaccaa tagagggcac tccaccacca
     2341 caaatgccac attttagaaa gtaagaccca gctttcttgt acaaagtggt gatgggcggc
     2401 cgcttccctt tagtgagggt taatgcttcg agcagacatg ataagataca ttgatgagtt
     2461 tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc
     2521 tattgcttta tttgtaacca ttataagctg caataaacaa gttaacaaca acaattgcat
     2581 tcattttatg tttcaggttc agggggagat gtgggaggtt ttttaaagca agtaaaacct
     2641 ctacaaatgt ggtaaaatcc gataaggatc gatccgggct ggcgtaatag cgaagaggcc
     2701 cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggac gcgccctgta
     2761 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca
     2821 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct
     2881 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttaga gctttacggc
     2941 acctcgaccg caaaaaactt gatttgggtg atggttcacg tagtgggcca tcgccctgat
     3001 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc
     3061 aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc
     3121 cgatttcggc ctattggtta aaaaatgagc tgatttaaca aatatttaac gcgaatttta
     3181 acaaaatatt aacgtttaca atttcgcctg atgcggtatt ttctccttac gcatctgtgc
     3241 ggtatttcac accgcatacg cggatctgcg cagcaccatg gcctgaaata acctctgaaa
     3301 gaggaacttg gttaggtacc ttctgaggcg gaaagaacca gctgtggaat gtgtgtcagt
     3361 tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     3421 attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
     3481 gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc
     3541 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
     3601 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg
     3661 gaggcctagg cttttgcaaa aagcttgatt cttctgacac aacagtctcg aacttaaggc
     3721 tagagccacc atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga
     3781 gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt
     3841 ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct
     3901 gaatgaactg caggacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg
     3961 cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt
     4021 gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc
     4081 tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc
     4141 gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga
     4201 tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg
     4261 catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat
     4321 ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg
     4381 ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc
     4441 tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta
     4501 tcgccttctt gacgagttct tctgagcggg actctggggt tcgaaatgac cgaccaagcg
     4561 acgcccaacc tgccatcacg atggccgcaa taaaatatct ttattttcat tacatctgtg
     4621 tgttggtttt ttgtgtgaat cgatagcgat aaggatctgc cccggcgccg atccgcgtat
     4681 ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagccc cgacacccgc
     4741 caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag
     4801 ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg
     4861 cgagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg
     4921 tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
     4981 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     5041 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
     5101 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
     5161 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
     5221 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
     5281 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca
     5341 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
     5401 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
     5461 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
     5521 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
     5581 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
     5641 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
     5701 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
     5761 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
     5821 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
     5881 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
     5941 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     6001 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     6061 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     6121 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     6181 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     6241 tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     6301 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     6361 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     6421 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     6481 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     6541 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     6601 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     6661 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     6721 tttgctggcc ttttgctcac atggctcgac agatct
//
