LOCUS       40924_22433        4920 bp DNA     circular SYN 13-JAN-2022
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4920)
  AUTHORS   caoheibi
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 4920)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4920
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             76..630
                     /codon_start=1
                     /label=hGLuc
                     /note="secreted Gaussia luciferase"
                     /translation="MGVKVLFALICIAVAEAKPTENNEDFNIVAVASNFATTDLDADRG
                     KLPGKKLPLEVLKEMEANARKAGCTRGCLICLSHIKCTPKMKKFIPGRCHTYEGDKESA
                     QGGIGEAIVDIPEIPGFKDLEPMEQFIAQVDLCVDCTTGCLKGLANVQCSDLLKKWLPQ
                     RCATFASKIQGQVDKIKGAGGD"
     rep_origin      786..1214
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        1228..1558
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             1625..2416
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    2593..2726
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2763..2779)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2787..2803)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2811..2841)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2856..2877)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3165..3753)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3927..4784)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4785..4889)
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatcttgg aattctgcag atatcctcga gcccaagctt ggtaccgagc
       61 tcggatccag ccaccatggg agtcaaagtt ctgtttgccc tgatctgcat cgctgtggcc
      121 gaggccaagc ccaccgagaa caacgaagac ttcaacatcg tggccgtggc cagcaacttc
      181 gcgaccacgg atctcgatgc tgaccgcggg aagttgcccg gcaagaagct gccgctggag
      241 gtgctcaaag agatggaagc caatgcccgg aaagctggct gcaccagggg ctgtctgatc
      301 tgcctgtccc acatcaagtg cacgcccaag atgaagaagt tcatcccagg acgctgccac
      361 acctacgaag gcgacaaaga gtccgcacag ggcggcatag gcgaggcgat cgtcgacatt
      421 cctgagattc ctgggttcaa ggacttggag cccatggagc agttcatcgc acaggtcgat
      481 ctgtgtgtgg actgcacaac tggctgcctc aaagggcttg ccaacgtgca gtgttctgac
      541 ctgctcaaga agtggctgcc gcaacgctgt gcgacctttg ccagcaagat ccagggccag
      601 gtggacaaga tcaagggggc cggtggtgac taagcggccg ctcgagcatg catctagaaa
      661 taattcttac tgtcatgcca agtaagatgc ttttctgtgc tgcaatagca ggcatgctgg
      721 ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agctggggct ctagggggta
      781 tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
      841 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
      901 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg ggcatccctt tagggttccg
      961 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag
     1021 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
     1081 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga
     1141 tttataaggg attttgggga tttcggccta ttggttaaaa aatgagctga tttaacaaaa
     1201 atttaacgcg aattaattct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc
     1261 tccccaggca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg
     1321 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     1381 aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
     1441 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctctgc
     1501 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa
     1561 gctcccggga gcttgtatat ccattttcgg atctgatcaa gagacaggat gaggatcgtt
     1621 tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct
     1681 attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct
     1741 gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga
     1801 actgcaggac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc
     1861 tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg
     1921 gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc
     1981 aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca
     2041 tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga
     2101 cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg cgcgcatgcc
     2161 cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga
     2221 aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca
     2281 ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg
     2341 cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct
     2401 tcttgacgag ttcttctgag cgggactctg gggttcgaaa tgaccgacca agcgacgccc
     2461 aacctgccat cacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga
     2521 atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc
     2581 ttcgcccacc ccaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
     2641 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
     2701 atcaatgtat cttatcatgt ctgtataccg tcgacctcta gctagagctt ggcgtaatca
     2761 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
     2821 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
     2881 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
     2941 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
     3001 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     3061 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     3121 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     3181 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     3241 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     3301 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcaa
     3361 tgctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     3421 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     3481 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     3541 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     3601 agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     3661 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     3721 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     3781 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     3841 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     3901 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     3961 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     4021 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
     4081 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     4141 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     4201 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
     4261 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     4321 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     4381 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     4441 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     4501 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
     4561 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     4621 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     4681 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     4741 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
     4801 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
     4861 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
//
