LOCUS       40924_22423        7137 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Integration vector pGlt-Kan, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7137)
  AUTHORS   Middleton R, Hofmeister A.
  TITLE     New shuttle vectors for ectopic insertion of genes into Bacillus 
            subtilis
  JOURNAL   Plasmid 51 (3), 238-245 (2004)
  PUBMED    15109830
REFERENCE   2  (bases 1 to 7137)
  AUTHORS   Middleton RH, Hofmeister AEM.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2003) Plant and Microbial Biology, University of 
            California, Berkeley, 281 Koshland Hall, Berkeley, CA 94704, USA
REFERENCE   3  (bases 1 to 7137)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7137)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plasmid"; 
            date: "2004"; volume: "51"; issue: "3"; pages: "238-245"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (13-NOV-2003) Plant and Microbial Biology, University of California,
            Berkeley, 281 Koshland Hall, Berkeley, CA 94704, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7137
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(57..1883)
                     /note="glutamate synthase large subunit (gltA) from
                     Bacillus subtilis"
     CDS             complement(2347..3138)
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     misc_feature    complement(4112..4825)
                     /note="glutamate synthase large subunit (gltA) from
                     Bacillus subtilis"
     promoter        5231..5335
                     /label=AmpR promoter
     CDS             5336..6193
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      6367..6955
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcaggctgct
       61 gtattctgct ttggcttcgt gttggcctca aaagcgaaca tgatggcttc ttcatctgat
      121 aaacctgcag ctttttgctc ttcgatgctt gcgagcattt gtttatagtt tttcggaatg
      181 actttgacga attttttcac actgtcttcc cattgatcta gcagatcttc tgctttttgg
      241 ctgtttgtat acgcagtatg tctttcaagc attgctttga tttgctggat ttctttctca
      301 tcctctaatg attcaaataa aatcatctca aggttgcatt tgcgtttaaa ccgtttgaca
      361 tcttcggtca gtacataagc gattccgccg gacatacctg ccgcaaagtt tttgcctaca
      421 tcaccgagaa cgacaacgct gccgccagtc atgtattcac agccgtggtc gccgatgcct
      481 tctacaacta cgttcacacc gctgtttcga acggcaaagc gttcaccagc acgcccgtta
      541 atatatgctt ctccgcttgt cgcaccgtaa aaagccacgt tgccgataat gacattgtca
      601 tccgaagcag agttgaatcc ttctgatgac ttgacgatga tttttccgcc agaaagccct
      661 tttccgacgt aatcatttga gtctccgtcc aaataaagcg tcatcccttt aggaacgaaa
      721 gctccaaagc tttggccggc tgatccggta aagtgcagct tgattgtatc ttcaggaaga
      781 ccttcttctc cgtaacgctt tgagatttca ctgccggtta tcgttccggc tacacgattt
      841 gtattattga tttcaatcga aatatcagct tcttttccag attcgatggc ttcttgtacg
      901 gccgggagaa ttgttgtaat atcaagtgat tgatcaattt tatgattttg cggcgattgg
      961 aacgtccgca ccccttcagg ctgataaaga agggtagaca aatcaagctg gcttgctttc
     1021 cagtgctcct ttgcccgttc acttgcatga agtacatcag tgcggccgat catttcatca
     1081 aatgtcttga agcctaacgc agccatgtac tcacgaactt cttcggcaat gaacagcata
     1141 tagttcacaa tatggtcagg atctcccatg aactttttgc gaagctctgg attttgtgtc
     1201 gctacaccga cagggcatgt atccaaatgg caggcacgca tcatgacaca gccgagtaca
     1261 actaacggag ccgttgcgaa accaaattct tcggcgccga gcaaggcagc catcacaacg
     1321 tcgcggcccg tcatgagctt tccatctgtt tctaatacaa cacggtcacg aagtccgttc
     1381 agcattagtg tttgatgtgc ttctgcaagg ccaagctccc acggaagccc tgtatgttta
     1441 atactggttt tcggagaagc gcctgtacct ccgtcatagc cgctgatcac aatgacatct
     1501 gcagtcgctt tggcaacacc tgcagcgatt gttcctacac ctgcttttga caccagcttt
     1561 acgctgattc ttgcgtcacg gttggcattt ttcaaatcgt ggatcagctg ggctaaatcc
     1621 tcaatcgaat aaatgtcatg gtgtggcgga ggtgagatta atccgacacc tggcgttgac
     1681 ccacggacat cggcaaccca tggatatacc ttgttgccag gaagctgccc gccttcaccc
     1741 ggcttagcac cttgagccat tttaatctgc agctcatcag cattgacgag gtaatggctt
     1801 ttgacaccaa accgtccgga tgcaatttgt ttgatcgcac ttcttctatc atcgccgttc
     1861 tcatctggaa caaagcgttt ggggcgcgcc taaacggata tcatcatcgc tcatccatgt
     1921 cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg
     1981 ttgagcaccg ccgccgcaag gaatggtgca tgccgacgtc ctcccttatg cgactcctgc
     2041 attaggaagc agcccagtag taggttgagg ccgaagagca ccgccgccgc aaggaatggt
     2101 gcatgcctgc aggtcctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca
     2161 gttgcgcagc ctgaatggcg aatggcgata caaattcctc gtaggcgctc gggaccccta
     2221 tctagcgaac ttttagaaaa gatataaaac atcagagtat ggacagttgc ggatgtactt
     2281 cagaaaagat tagatgtcta aaaagcttgt agttaaagct ttttagacat ctaaatctag
     2341 gtactaaaac aattcatcca gtaaaatata atattttatt ttctcccaat caggcttgat
     2401 ccccagtaag tcaaaaaata gctcgacata ctgttcttcc ccgatatcct ccctgatcga
     2461 ccggacgcag aaggcaatgt cataccactt gtccgccctg ccgcttctcc caagatcaat
     2521 aaagccactt actttgccat ctttcacaaa gatgttgctg tctcccaggt cgccgtggga
     2581 aaagacaagt tcctcttcgg gcttttccgt ctttaaaaaa tcatacagct cgcgcggatc
     2641 tttaaatgga gtgtcttctt cccagttttc gcaatccaca tcggccagat cgttattcag
     2701 taagtaatcc aattcggcta agcggctgtc taagctattc gtatagggac aatccgatat
     2761 gtcgatggag tgaaagagcc tgatgcactc cgcatacagc tcgataatct tttcagggct
     2821 ttgttcatct tcatactctt ccgagcaaag gacgccatcg gcctcactca tgagcagatt
     2881 gctccagcca tcatgccgtt caaagtgcag gacctttgga acaggcagct ttccttccag
     2941 ccatagcatc atgtcctttt cccgttccac atcataggtg gtccctttat accggctgtc
     3001 cgtcattttt aaatataggt tttcattttc tcccaccagc ttatatacct tagcaggaga
     3061 cattccttcc gtatctttta cgcagcggta tttttcgatc agttttttca attccggtga
     3121 tattctcatt ttagccattt attatttcct tcctcttttc tacagtattt aaagataccc
     3181 caagaagcta attataacaa gacgaactcc aattcactgt tccttgcatt ctaaaacctt
     3241 aaataccaga aaacagcttt ttcaaagttg ttttgaaagt tggcgtataa catagtatcg
     3301 acggagccga ttttgaaacc acaattatga tagaatttac aagctataag gttattgtcc
     3361 tgggtttcaa gcattagtcc atgcaagttt ttatgctttg cccattctat agatatattg
     3421 ataagcgcgc tgcctatgcc ttgccccctg aaatccttac atacggcgat atcttctata
     3481 taaaagatat attatcttat cagtattgtc aatatattca aggcaatctg cctcctcatc
     3541 ctcttcatcc tcttcgtctt ggtagctttt taaatatggc gcttcataga gtaattctgt
     3601 aaaggtccaa ttctcgtttt catacctcgg tataatctta cctatcacct caaatggttc
     3661 gctgggttta tcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc
     3721 gcatatgatc agatcttaag gcctaggtct agagtctttg ttttgacgcc attagcgtac
     3781 gtaacaatcc tcgttaaagg acaaggacct gagcggaagt gtatcgtaca gtagacggag
     3841 tatactagta tagtctatag tccgtggaat tattatattt atctccgacg atattctcat
     3901 cagtgaaatc caggggtacc gagctcgaat tcgataagct tctaggatcc tgagcgccgg
     3961 tcgctaccat taccagttgg tctggtgtca aaaataataa taaccgggca ggccatgtct
     4021 gcccgtattt cgcgtaagga aatccattat gtactatttc gatcagacca gtttttaatt
     4081 tgtgtgtttc catgtgtcca gtttggcgcg ccaaatagcg gttaggatgc gctctttccc
     4141 atgtagggaa tgtattggta ctgaagcgtg aatgaacaag cgcaaaagca gaaacgaatg
     4201 cttcgtcttg cagatcagaa taaaatgcat caacctgttc aggtgttaaa aggcctttgt
     4261 aaacaatggt ctggcttgaa agactggcaa aataaaaatc aagaccttcc gttactcccc
     4321 aattttcagc ctgtttacga atgacataca attttcgttc aaaagataaa ttgtccttta
     4381 gatcagaact tgcgccgata aatacctgac ggacaaacgg acagcttttt tgcgccactg
     4441 ttccgatttt tccgacattt acaggtacag ttctccatcc aagaacgact tggccttctt
     4501 gttcaattag tgcattgatt tgcttttcaa ttttttttct ttcatcttcc ttctgtgaga
     4561 aaaagaccat tcctaccccg taacgttctt tttctggcag attgatgttt ttgcactctt
     4621 ttctaaagaa agcatcaggg atttgaacca gtaagccggc tccgtctcct gtatccggat
     4681 cactgccttg ccctcctcta tggtctagct ggcaaagcat cttaagtcct tgtttgacaa
     4741 tgtcgtgagt ctgcttgccc tttaagtgtg catataggcc gattccgcat gcatcatgtt
     4801 caaattcagg acggtagaga ccttgagctg gcgtaatagc gaagaggccc gcaccgatcg
     4861 cccttcccaa cagttgcgca gcctgaatgg cgaatggcgc ctgatgcggt attttctcct
     4921 tacgcatctg tgcggtattt cacaccgcat atggtgcact ctcagtacaa tctgctctga
     4981 tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc
     5041 ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg
     5101 tcagaggttt tcaccgtcat caccgaaacg cgcgagacga aagggcctcg tgatacgcct
     5161 atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg
     5221 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
     5281 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
     5341 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
     5401 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
     5461 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
     5521 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat
     5581 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
     5641 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
     5701 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
     5761 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
     5821 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt
     5881 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
     5941 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
     6001 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg
     6061 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
     6121 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
     6181 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
     6241 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
     6301 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     6361 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     6421 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     6481 tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca
     6541 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     6601 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     6661 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     6721 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     6781 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     6841 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     6901 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     6961 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     7021 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
     7081 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaaga
//
