LOCUS       V005920                 6462 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V005920
VERSION     V005920
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6462)
  AUTHORS   Middleton R, Hofmeister A.
  TITLE     New shuttle vectors for ectopic insertion of genes into Bacillus
            subtilis
  JOURNAL   Plasmid 51 (3), 238-245 (2004)
   PUBMED   15109830
REFERENCE   2  (bases 1 to 6462)
  AUTHORS   Middleton RH, Hofmeister AEM.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2003) Plant and Microbial Biology, University of
            California, Berkeley, 281 Koshland Hall, Berkeley, CA 94704, USA
REFERENCE   3  (bases 1 to 6462)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6462)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plasmid";
            date: "2004"; volume: "51"; issue: "3"; pages: "238-245"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (13-NOV-2003) Plant and Microbial Biology, University of California,
            Berkeley, 281 Koshland Hall, Berkeley, CA 94704, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6462
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(57..1883)
                     /note="glutamate synthase large subunit (gltA) from
                     Bacillus subtilis"
     CDS             complement(2297..2944)
                     /gene="cat"
                     /label="Chloramphenicol acetyltransferase"
                     /note="Chloramphenicol acetyltransferase from
                     Staphylococcus aureus. Accession#: P00485"
     misc_feature    complement(3437..4150)
                     /note="glutamate synthase large subunit (gltA) from
                     Bacillus subtilis"
     promoter        4556..4660
                     /label="AmpR promoter"
     CDS             4661..5518
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      5692..6280
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
ORIGIN
        1 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcaggctgct
       61 gtattctgct ttggcttcgt gttggcctca aaagcgaaca tgatggcttc ttcatctgat
      121 aaacctgcag ctttttgctc ttcgatgctt gcgagcattt gtttatagtt tttcggaatg
      181 actttgacga attttttcac actgtcttcc cattgatcta gcagatcttc tgctttttgg
      241 ctgtttgtat acgcagtatg tctttcaagc attgctttga tttgctggat ttctttctca
      301 tcctctaatg attcaaataa aatcatctca aggttgcatt tgcgtttaaa ccgtttgaca
      361 tcttcggtca gtacataagc gattccgccg gacatacctg ccgcaaagtt tttgcctaca
      421 tcaccgagaa cgacaacgct gccgccagtc atgtattcac agccgtggtc gccgatgcct
      481 tctacaacta cgttcacacc gctgtttcga acggcaaagc gttcaccagc acgcccgtta
      541 atatatgctt ctccgcttgt cgcaccgtaa aaagccacgt tgccgataat gacattgtca
      601 tccgaagcag agttgaatcc ttctgatgac ttgacgatga tttttccgcc agaaagccct
      661 tttccgacgt aatcatttga gtctccgtcc aaataaagcg tcatcccttt aggaacgaaa
      721 gctccaaagc tttggccggc tgatccggta aagtgcagct tgattgtatc ttcaggaaga
      781 ccttcttctc cgtaacgctt tgagatttca ctgccggtta tcgttccggc tacacgattt
      841 gtattattga tttcaatcga aatatcagct tcttttccag attcgatggc ttcttgtacg
      901 gccgggagaa ttgttgtaat atcaagtgat tgatcaattt tatgattttg cggcgattgg
      961 aacgtccgca ccccttcagg ctgataaaga agggtagaca aatcaagctg gcttgctttc
     1021 cagtgctcct ttgcccgttc acttgcatga agtacatcag tgcggccgat catttcatca
     1081 aatgtcttga agcctaacgc agccatgtac tcacgaactt cttcggcaat gaacagcata
     1141 tagttcacaa tatggtcagg atctcccatg aactttttgc gaagctctgg attttgtgtc
     1201 gctacaccga cagggcatgt atccaaatgg caggcacgca tcatgacaca gccgagtaca
     1261 actaacggag ccgttgcgaa accaaattct tcggcgccga gcaaggcagc catcacaacg
     1321 tcgcggcccg tcatgagctt tccatctgtt tctaatacaa cacggtcacg aagtccgttc
     1381 agcattagtg tttgatgtgc ttctgcaagg ccaagctccc acggaagccc tgtatgttta
     1441 atactggttt tcggagaagc gcctgtacct ccgtcatagc cgctgatcac aatgacatct
     1501 gcagtcgctt tggcaacacc tgcagcgatt gttcctacac ctgcttttga caccagcttt
     1561 acgctgattc ttgcgtcacg gttggcattt ttcaaatcgt ggatcagctg ggctaaatcc
     1621 tcaatcgaat aaatgtcatg gtgtggcgga ggtgagatta atccgacacc tggcgttgac
     1681 ccacggacat cggcaaccca tggatatacc ttgttgccag gaagctgccc gccttcaccc
     1741 ggcttagcac cttgagccat tttaatctgc agctcatcag cattgacgag gtaatggctt
     1801 ttgacaccaa accgtccgga tgcaatttgt ttgatcgcac ttcttctatc atcgccgttc
     1861 tcatctggaa caaagcgttt ggggcgcgcc taaacggata tcatcatcgc tcatccatgt
     1921 cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg
     1981 ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg
     2041 gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga
     2101 gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg
     2161 ccggtgatgc cggccacgat gcgtccggcg tagaggatct ggagctgtaa tataaaaacc
     2221 ttcttcaact aacggggcag gttagtgaca ttagaaaacc gactgtaaaa agtacagtcg
     2281 gcattatctc atattataaa agccagtcat taggcctatc tgacaattcc tgaatagagt
     2341 tcataaacaa tcctgcatga taaccatcac aaacagaatg atgtacctgt aaagatagcg
     2401 gtaaatatat tgaattacct ttattaatga attttcctgc tgtaataatg ggtagaaggt
     2461 aattactatt attattgata tttaagttaa acccagtaaa tgaagtccat ggaataatag
     2521 aaagagaaaa agcattttca ggtataggtg ttttgggaaa caatttcccc gaaccattat
     2581 atttctctac atcagaaagg tataaatcat aaaactcttt gaagtcattc tttacaggag
     2641 tccaaatacc agagaatgtt ttagatacac catcaaaaat tgtataaagt ggctctaact
     2701 tatcccaata acctaactct ccgtcgctat tgtaaccagt tctaaaagct gtatttgagt
     2761 ttatcaccct tgtcactaag aaaataaatg cagggtaaaa tttatatcct tcttgtttta
     2821 tgtttcggta taaaacacta atatcaattt ctgtggttat actaaaagtc gtttgttggt
     2881 tcaaataatg attaaatatc tcttttctct tccaattgtc taaatcaatt ttattaaagt
     2941 tcatttgata tgcctcctaa atttttatct aaagtgaatt taggaggctt acttgtctgc
     3001 tttcttcatt agaatcaatc cttttttaaa agtcaatatt actgtaacat aaatatatat
     3061 tttaaaaata tcccacttta tccaattttc gtttgttgaa ctaatgggtg ctttagttga
     3121 agaataaaga ccacattaaa aaatgtggtc ttttgtgttt ttttaaagga tttgagcgta
     3181 gcgaaaaatc cttttctttc ttatcttgat aataagggta actattgccg atgataagct
     3241 gtcaaacatg agaattcgat aagcttctag gatcctgagc gccggtcgct accattacca
     3301 gttggtctgg tgtcaaaaat aataataacc gggcaggcca tgtctgcccg tatttcgcgt
     3361 aaggaaatcc attatgtact atttcgatca gaccagtttt taatttgtgt gtttccatgt
     3421 gtccagtttg gcgcgccaaa tagcggttag gatgcgctct ttcccatgta gggaatgtat
     3481 tggtactgaa gcgtgaatga acaagcgcaa aagcagaaac gaatgcttcg tcttgcagat
     3541 cagaataaaa tgcatcaacc tgttcaggtg ttaaaaggcc tttgtaaaca atggtctggc
     3601 ttgaaagact ggcaaaataa aaatcaagac cttccgttac tccccaattt tcagcctgtt
     3661 tacgaatgac atacaatttt cgttcaaaag ataaattgtc ctttagatca gaacttgcgc
     3721 cgataaatac ctgacggaca aacggacagc ttttttgcgc cactgttccg atttttccga
     3781 catttacagg tacagttctc catccaagaa cgacttggcc ttcttgttca attagtgcat
     3841 tgatttgctt ttcaattttt tttctttcat cttccttctg tgagaaaaag accattccta
     3901 ccccgtaacg ttctttttct ggcagattga tgtttttgca ctcttttcta aagaaagcat
     3961 cagggatttg aaccagtaag ccggctccgt ctcctgtatc cggatcactg ccttgccctc
     4021 ctctatggtc tagctggcaa agcatcttaa gtccttgttt gacaatgtcg tgagtctgct
     4081 tgccctttaa gtgtgcatat aggccgattc cgcatgcatc atgttcaaat tcaggacggt
     4141 agagaccttg agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt
     4201 gcgcagcctg aatggcgaat ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg
     4261 tatttcacac cgcatatggt gcactctcag tacaatctgc tctgatgccg catagttaag
     4321 ccagccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc
     4381 atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc
     4441 gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa
     4501 tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg
     4561 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     4621 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
     4681 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
     4741 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
     4801 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
     4861 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
     4921 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
     4981 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
     5041 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
     5101 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
     5161 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
     5221 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
     5281 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
     5341 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
     5401 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
     5461 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
     5521 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
     5581 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     5641 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     5701 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     5761 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     5821 gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     5881 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     5941 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     6001 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     6061 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     6121 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     6181 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     6241 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     6301 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
     6361 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
     6421 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa ga
//
