LOCUS       40924_22413        7960 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Reporter vector pGlowRed, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7960)
  AUTHORS   Krol JE, Rogers LM, Krone SM, Top EM.
  TITLE     Dual reporter system for in situ detection of plasmid transfer under
            aerobic and anaerobic conditions
  JOURNAL   Appl. Environ. Microbiol. 76 (13), 4553-4556 (2010)
  PUBMED    20453134
REFERENCE   2  (bases 1 to 7960)
  AUTHORS   Krol JE.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2010) Biological Sciences, University of Idaho, 
            P.O. Box 445031, Moscow, ID 83844, USA
REFERENCE   3  (bases 1 to 7960)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7960)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl. 
            Environ. Microbiol."; date: "2010"; volume: "76"; issue: "13"; 
            pages: "4553-4556"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (31-MAR-2010) Biological Sciences, University of Idaho, P.O. Box 
            445031, Moscow, ID 83844, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7960
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             413..430
                     /label=6xHis
                     /note="6xHis affinity tag"
     terminator      497..544
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(1420..2079)
                     /label=pBBR1 Rep
                     /note="replication protein for the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica"
     rep_origin      complement(2080..2849)
                     /direction=LEFT
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
     promoter        4242..4344
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     CDS             4345..4977
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     CDS             complement(5433..6107)
                     /label=DsRed1
                     /note="wild-type DsRed"
     RBS             6114..6125
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     promoter        complement(6175..6193)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(6211..6227)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(6235..6251)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6259..6289)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(6304..6325)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     CDS             complement(6699..7490)
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     promoter        7874..7892
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    7893..7917
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             7932..7954
                     /label=RBS
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 tatggcgtcg ttccagtcgt tcggcatccc gggccagctg gaagtcatca agaaggcgct
       61 ggatcacgtg cgcgtcggcg tggtcatcac cgatcccgcg ctggaagata acccgatcgt
      121 ctacgtgaac cagggcttcg tgcagatgac cggctacgag accgaggaaa tcctgggcaa
      181 gaacgcgcgc ttcctccagg ggaagcacac cgatccggcg gaagtggaca acatccgcac
      241 cgcgctgcaa aataaagaac cggtcaccgt gcagatccag aactacaaga aggacggcac
      301 gatgttctgg aacgaactga acatcgatcc gatggaaatc gaggataaga cgtatttcgt
      361 cggcatccag aacgacatca ccaagcagaa ggaatatgaa aagctgctcg agcaccacca
      421 ccaccaccac tgagatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc
      481 caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt
      541 tttgctgaaa ggaggaacta tatccggatt ggcgaatggg acgcgccctg tagcggcgca
      601 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
      661 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
      721 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
      781 cccaaaaaac ttgattaggg tgatggttca ccgcccttcc caacagttgc gcagcctgaa
      841 tggcgaatgg aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa
      901 atcagctcat tttttaacca ataggccgac tgcgatgagt ggcagggcgg ggcgtaattt
      961 ttttaaggca gttattggtg cccttaaacg cctggtgcta cgcctgaata agtgataata
     1021 agcggatgaa tggcagaaat tcgaaagcaa attcgacccg gtcgtcggtt cagggcaggg
     1081 tcgttaaata gccgcttatg tctattgctg gtttaccggt ttattgacta ccggaagcag
     1141 tgtgaccgtg tgcttctcaa atgcctgagg ccagtttgct caggctctcc ccgtggaggt
     1201 aataattgac gatatgatca tttattctgc ctcccagagc ctgataaaaa cggtgaatcc
     1261 gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga
     1321 cgcaacgcgg ggaggcagac aaggtatagg gcggcgaggc ggctacagcc gatagtctgg
     1381 aacagcgcac ttacgggttg ctgcgcaacc caagtgctac cggcgcggca gcgtgacccg
     1441 tgtcggcggc tccaacggct cgccatcgtc cagaaaacac ggctcatcgg gcatcggcag
     1501 gcgctgctgc ccgcgccgtt cccattcctc cgtttcggtc aaggctggca ggtctggttc
     1561 catgcccgga atgccgggct ggctgggcgg ctcctcgccg gggccggtcg gtagttgctg
     1621 ctcgcccgga tacagggtcg ggatgcggcg caggtcgcca tgccccaaca gcgattcgtc
     1681 ctggtcgtcg tgatcaacca ccacggcggc actgaacacc gacaggcgca actggtcgcg
     1741 gggctggccc cacgccacgc ggtcattgac cacgtaggcc gacacggtgc cggggccgtt
     1801 gagcttcacg acggagatcc agcgctcggc caccaagtcc ttgactgcgt attggaccgt
     1861 ccgcaaagaa cgtccgatga gcttggaaag tgtcttctgg ctgaccacca cggcgttctg
     1921 gtggcccatc tgcgccacga ggtgatgcag cagcattgcc gccgtgggtt tcctcgcaat
     1981 aagcccggcc cacgcctcat gcgctttgcg ttccgtttgc acccagtgac cgggcttgtt
     2041 cttggcttga atgccgattt ctctggactg cgtggccatg cttatctcca tgcggtaggg
     2101 tgccgcacgg ttgcggcacc atgcgcaatc agctgcaact tttcggcagc gcgacaacaa
     2161 ttatgcgttg cgtaaaagtg gcagtcaatt acagattttc tttaacctac gcaatgagct
     2221 attgcggggg gtgccgcaat gagctgttgc gtacccccct tttttaagtt gttgattttt
     2281 aagtctttcg catttcgccc tatatctagt tctttggtgc ccaaagaagg gcacccctgc
     2341 ggggttcccc cacgccttcg gcgcggctcc ccctccggca aaaagtggcc cctccggggc
     2401 ttgttgatcg actgcgcggc cttcggcctt gcccaaggtg gcgctgcccc cttggaaccc
     2461 ccgcactcgc cgccgtgagg ctcggggggc aggcgggcgg gcttcgcctt cgactgcccc
     2521 cactcgcata ggcttgggtc gttccaggcg cgtcaaggcc aagccgctgc gcggtcgctg
     2581 cgcgagcctt gacccgcctt ccacttggtg tccaaccggc aagcgaagcg cgcaggccgc
     2641 aggccggagg cttttcccca gagaaaatta aaaaaattga tggggcaagg ccgcaggccg
     2701 cgcagttgga gccggtgggt atgtggtcga aggctgggta gccggtgggc aatccctgtg
     2761 gtcaagctcg tgggcaggcg cagcctgtcc atcagcttgt ccagcagggt tgtccacggg
     2821 ccgagcgaag cgagccagcc ggtggccgct cgcggccatc gtccacatat ccacgggctg
     2881 gcaagggagc gcagcgaccg cgcagggcga agcccggaga gcaagcccgt agggcgccgc
     2941 agccgccgta ggcggtcacg actttgcgaa gcaaagtcta gtgagtatac tcaagcattg
     3001 agtggcccgc cggaggcacc gccttgcgct gcccccgtcg agccggttgg acaccaaaag
     3061 ggaggggcag gcatggcggc atacgcgatc atgcgatgca agaagctggc gaaaatgggc
     3121 aacgtggcgg ccagtctcaa gcacgcctac cgcgagcgcg agacgcccaa cgctgacgcc
     3181 agcaggacgc cagagaacga gcactgggcg gccagcagca ccgatgaagc gatgggccga
     3241 ctgcgcgagt tgctgccaga gaagcggcgc aaggacgctg tgttggcggt cgagtacgtc
     3301 atgacggcca gcccggaatg gtggaagtcg gccagccaag aacagcaggc ggcgttcttc
     3361 gagaaggcgc acaagtggct ggcggacaag tacggggcgg atcgcatcgt gacggccagc
     3421 atccaccgtg acgaaaccag cccgcacatg accgcgttcg tggtgccgct gacgcaggac
     3481 ggcaggctgt cggccaagga gttcatcggc aacaaagcgc agatgacccg cgaccagacc
     3541 acgtttgcgg ccgctgtggc cgatctaggg ctgcaacggg gcatcgaggg cagcaaggca
     3601 cgtcacacgc gcattcaggc gttctacgag gccctggagc ggccaccagt gggccacgtc
     3661 accatcagcc cgcaagcggt cgagccacgc gcctatgcac cgcagggatt ggccgaaaag
     3721 ctgggaatct caaagcgcgt tgagacgccg gaagccgtgg ccgaccggct gacaaaagcg
     3781 gttcggcagg ggtatgagcc tgccctacag gccgccgcag gagcgcgtga gatgcgcaag
     3841 aaggccgatc aagcccaaga gacggcccga gaccttcggg agcgcctgaa gcccgttctg
     3901 gacgccctgg ggccgttgaa tcgggatatg caggccaagg ccgccgcgat catcaaggcc
     3961 gtgggcgaaa agctgctgac ggaacagcgg gaagtccagc gccagaaaca ggcccagcgc
     4021 cagcaggaac gcgggcgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca
     4081 ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtcttcgaa
     4141 taaatacctg tgacggaaga tcacttcgca gaataaataa atcctggtgt ccctgttgat
     4201 accgggaagc cctgggccaa cttttggcga aaatgagacg ttgatcggca cgtaagaggt
     4261 tccaactttc accataatga aataagatca ctaccgggcg tattttttga gttatcgaga
     4321 ttttcaggag ctaaggaagc taaaatggag aaaaaaatca ctggatatac caccgttgat
     4381 atatcccaat ggcatcgtaa agaacatttt gaggcatttc agtcagttgc tcaatgtacc
     4441 tataaccaga ccgttcagct ggatattacg gcctttttaa agaccgtaaa gaaaaataag
     4501 cacaagtttt atccggcctt tattcacatt cttgcccgcc tgatgaatgc tcatccggaa
     4561 ttccgtatgg caatgaaaga cggtgagctg gtgatatggg atagtgttca cccttgttac
     4621 accgttttcc atgagcaaac tgaaacgttt tcatcgctct ggagtgaata ccacgacgat
     4681 ttccggcagt ttctacacat atattcgcaa gatgtggcgt gttacggtga aaacctggcc
     4741 tatttcccta aagggtttat tgagaatatg tttttcgtct cagccaatcc ctgggtgagt
     4801 ttcaccagtt ttgatttaaa cgtggccaat atggacaact tcttcgcccc cgttttcacc
     4861 atgggcaaat attatacgca aggcgacaag gtgctgatgc cgctggcgat tcaggttcat
     4921 catgccgttt gtgatggctt ccatgtcggc agaatgctta atgaattaca acagttttta
     4981 tgcatgcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttatagtcat
     5041 gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgaga
     5101 tcccggtgcc taatgagtga gctaacttac attaattgcg ttgcgctcac tgcccgcttt
     5161 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
     5221 cggtttgcgt attgggcgcc agggtggttt ttcttttcac cagtgagacg ggcaacagct
     5281 gattgccctt caccgcctgg ccctgagaga gttgcagcaa gcggtccacg ctggtttgcc
     5341 ccagcaggcg aaaatcctgt ttgatggtgg ttaacggcgg gatataacat gagctgtctt
     5401 cggtatcgtc gtatcccact accgagatgt taaaggaaca gatggtggcg tccctcggtt
     5461 ctttcatact gctcaacgat tgtatagtct tcgttgtggc ttgttatatc cagtttggag
     5521 tcaacatagt agtaccctgg tagctgcaca ggcttctttg ccatgtaaat acttttgaat
     5581 tcaactaggt aatgaccacc gtctttcagc ttcagagcct tatgaatctc tcctttcaac
     5641 acgccatcac gaggatacaa acgctcagtg ctggcttccc agcccattgt cttcttttgc
     5701 ataacaggtc catcggaagg aaagttcacg ccaatgaact tgaccttgta gatgaaacag
     5761 ccatcctgca aactggaatc ctgggttaca gtaacgacgc caccgtcttc aaagttcatg
     5821 accctttccc atttaaatcc ttcaggaaat gacagctttt tatagtctgg tatgtcggca
     5881 gggtgcttga catatacctt gcttccatac tgaaattgtg gtgacaaaat atcccaagca
     5941 aatggcaaag gtcccccctt ggttacctta agctttacgg tattgtggcc ttcgtatggc
     6001 ctcccctctc cttcgccttc tatttcaaac tcgtgcccat tgaccgttcc ttccatgcga
     6061 accttaaacc tcatgaactc cttgataaca ttcttggaag accgcatgct taatttctcc
     6121 tctttaattc ggggatcctc tagagtcgac ctgcaggcat gcaagcttga gtattctata
     6181 gtgtcaccta aatagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
     6241 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
     6301 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     6361 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     6421 tattgggcgc tatccgcacc aacgcgcagc ccggactcgg taatggcgcg cattgcgccc
     6481 agcgccatct gatcgttggc aaccagcatc gcagtgggaa cgatgccctc attcagcatt
     6541 tgcatggttt gttgaaaacc ggacatggca ctccagtcgc cttcccgttc cgctatcggc
     6601 tgaatttgat tgcgagtgag atatttatgc cagccagcca gacgcagacg cgccgagaca
     6661 gaacttaatg aattccgcga accccagagt cccgctcaga agaactcgtc aagaaggcga
     6721 tagaaggcga tgcgctgcga atcgggagcg gcgataccgt aaagcacgag gaagcggtca
     6781 gcccattcgc cgccaagctc ttcagcaata tcacgggtag ccaacgctat gtcctgatag
     6841 cggtccgcca cacccagccg gccacagtcg atgaatccag aaaagcggcc attttccacc
     6901 atgatattcg gcaagcaggc atcgccatgg gtcacgacga gatcctcgcc gtcgggcatc
     6961 cgcgccttga gcctggcgaa cagttcggct ggcgcgagcc cctgatgctc ttcgtccaga
     7021 tcatcctgat cgacaagacc ggcttccatc cgagtacgtg ctcgctcgat gcgatgtttc
     7081 gcttggtggt cgaatgggca ggtagccgga tcaagcgtat gcagccgccg cattgcatca
     7141 gccatgatgg atactttctc ggcaggagca aggtgagatg acaggagatc ctgccccggc
     7201 acttcgccca atagcagcca gtcccttccc gcttcagtga caacgtcgag cacagctgcg
     7261 caaggaacgc ccgtcgtggc cagccacgat agccgcgctg cctcgtcttg gagttcattc
     7321 agggcaccgg acaggtcggt cttgacaaaa agaaccgggc gcccctgcgc tgacagccgg
     7381 aacacggcgg catcagagca gccgattgtc tgttgtgccc agtcatagcc gaatagcctc
     7441 tccacccaag cggccggaga acctgcgtgc aatccatctt gttcaatcat gcgaaacgat
     7501 cctcatcctg tctcttgatc agatcttgat cccctgcgcc atcagatcct tggcggcaag
     7561 aaagccatcc agtttacttt gcagggcttc ccaaccttac cagagggcgc cccagctggc
     7621 aattccggtt cgcttgctgt ccataaaacc gcccagtcta gctatcgcca tgtaagccca
     7681 ctgcaagcta cctgctttct ctttgcgctt gcgttttccc ttgtccagat agcccagtag
     7741 ctgacattca tccggggtca gcaccgtttc tgcggactgg ctttctacgt gttccgcttc
     7801 ctttagcagc ccttgcgccc tgagtgcttg cggcagcgtg aagctagcgg aattgatctc
     7861 gatcccgcga aattaatacg actcactata ggggaattgt gagcggataa caattcccct
     7921 ctagaaataa ttttgtttaa ctttaagaag gagatataca
//
