LOCUS       40924_21010        5399 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Mammalian expression vector pGal4-p65f520-550, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5399)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5399)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 5399)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5399)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927, 
            9052, BELGIUM"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5399
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    complement(766..900)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    complement(933..1723)
                     /label=3UTR
                     /note="3UTR"
     CDS             complement(1724..1819)
                     /codon_start=1
                     /note="unnamed protein product; p65f"
                     /protein_id="SJL87665.1"
                     /translation="PGLPNGLLSGDEDFSSIADMDFSALLSQISS"
     misc_feature    complement(1820..1828)
                     /label=link
                     /note="link"
     CDS             complement(1829..2269)
                     /label=GAL4 DNA binding domain
                     /note="DNA binding domain of the GAL4 transcriptional
                     activator"
     promoter        complement(2411..2672)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     promoter        2997..3326
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      complement(3572..4160)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4334..5191)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(5192..5296)
                     /label=AmpR promoter
ORIGIN
        1 ttcctttgcc taatttaaat gaggacttaa cctgtggaaa tattttgatg tgggaagctg
       61 ttactgttaa aactgaggtt attggggtaa ctgctatgtt aaacttgcat tcagggacac
      121 aaaaaactca tgaaaatggt gctggaaaac ccattcaagg gtcaaatttt catttttttg
      181 ctgttggtgg ggaacctttg gagctgcagg gtgtgttagc aaactacagg accaaatatc
      241 ctgctcaaac tgtaacccca aaaaatgcta cagttgacag tcagcagatg aacactgacc
      301 acaaggctgt tttggataag gataatgctt atccagtgga gtgctgggtt cctgatccaa
      361 gtaaaaatga aaacactaga tattttggaa cctacacagg tggggaaaat gtgcctcctg
      421 ttttgcacat tactaacaca gcaaccacag tgcttcttga tgagcagggt gttgggccct
      481 tgtgcaaagc tgacagcttg tatgtttctg ctgttgacat ttgtgggctg tttaccaaca
      541 cttctggaac acagcagtgg aagggacttc ccagatattt taaaattacc cttagaaagc
      601 ggtctgtgaa aaacccctac ccaatttcct ttttgttaag tgacctaatt aacaggagga
      661 cacagagggt ggatgggcag cctatgattg gaatgtcctc tcaagtagag gaggttaggg
      721 tttatgagga cacagaggag cttcctgggg atcgctcgat cgagcgatcc agacatgata
      781 agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt
      841 tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt
      901 aattccgcca cggatcaagc ttgatatcga attccttttt tttttttttt cagcttggca
      961 acagctttat tagttcagag tagaaagagc aagagtccaa gtgctttgat tgttcactaa
     1021 aaactatgcc tcctgactgg agagctgcca gcctgctctc ccccactctt aacaacttac
     1081 cctactatta aggcacttga gaagagggga gagcaaggaa gtccagacca aaccccttct
     1141 ggatcctgga gagagccagt gctgttgcac tgtttccttc agccatggtt gagcaaggaa
     1201 agagccggca gagacctctg tagggcagga aggcagcccc tcaaacgctg gtgttaggca
     1261 cagggacaat gccagtgcca tacaggggct ggtatctggg gcgttatttt gattaagctg
     1321 taatgaatcc atgatggaag acacttgata aggctttgtg ggctcaaagg ccttacctcc
     1381 agcctgcttc tgtctctagg agagtaccag aagctggagg atggggatgg gggaccccac
     1441 agttccctac agagaaggga gctgaccatc aggacagggg aaaagtttga gtttccccag
     1501 ctcccccctt tccagagaag ttaatgcttc tgcttaagca cctccaaaaa gagagagaga
     1561 tacagatact gacaataaaa gaataaaata tggctccccc ctccaaggaa gacatccaca
     1621 aagttggggg cagttggaac acaccccacc agaatccgta agtgcttttg gagggcttca
     1681 atcccctgca accagtgctc tggggagggc aggcgtcacc cccttaggag ctgatctgac
     1741 tcagcagggc tgagaagtcc atgtccgcaa tggaggagaa gtcttcatct cctgaaagga
     1801 ggccattggg gagccccggg aattccggcg atacagtcaa ctgtctttga cctttgttac
     1861 tactctcttc cgatgatgat gtcgcactta ttctatgctg tctcaatgtt agaggcatat
     1921 cagtctccac tgaagccaat ctatctgtga cggcatcttt attcacatta tcttgtacaa
     1981 ataatcctgt taacaatgct tttatatcct gtaaagaatc cattttcaaa atcatgtcaa
     2041 ggtcttctcg aggaaaaatc agtagaaata gctgttccag tctttctagc cttgattcca
     2101 cttctgtcag atgtgcccta gtcagcggag accttttggt tttgggagag tagcgacact
     2161 cccagttgtt cttcagacac ttggcgcact tcggtttttc tttggagcac ttgagctttt
     2221 taagtcggca aatatcgcat gcttgttcga tagaagacag tagcttcaga tctggctgct
     2281 gggagtccat cagtgaatat caactctggc agaggagccg ctcgcccgcc accgtccgca
     2341 gttcccgccg cagccgagat aaacaactta gcttgtgaac gcagaaggag caggaggtac
     2401 ccagcttgga ggtgcacacc aatgtggtga atggtcaaat ggcgtttatt gtatcgagct
     2461 aggcacttaa atacaatatc tctgcaatgc ggaattcagt ggttcgtcca atccatgtca
     2521 gacctgtctg ttgccttcct aataaggcac gatcgtacca ccttacttcc accaatcggc
     2581 atgcacggtg ctttttctct ccttgtaagg catgttgcta actcatcgtt accatgttgc
     2641 aagactacaa gtgtattgca taagactaca tttccccctc cctatgcaaa agcgaaacta
     2701 ctatatcctg aggggactcc taaccgcgta caaccgaagc cccgcttttc gcctaaacac
     2761 accctagtcc cctcagatac gcgtatatct ggcccgtaca tcgcgaagca gcgcaaaacg
     2821 cctaacccta agcagattct tcatgcaatt gtcggtcaag ccttgccttg ttgtagctta
     2881 aattttgctc gcgcactact cagcgacctc caacacacaa gcagggagca gatactggct
     2941 taactatgcg gcatcagagc agattgtact gagagtgcac catatggctg tggaatgtgt
     3001 gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
     3061 atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta
     3121 tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc
     3181 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta
     3241 tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct
     3301 tttttggagg cctaggcttt tgcaaaaagc tccatatgcg gtgtgaaata ccgcacagat
     3361 gcgtaaggag aaaataccgc atcaggcgct cttccgcttc ctcgctcact gactcgctgc
     3421 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     3481 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     3541 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     3601 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     3661 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     3721 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     3781 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     3841 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     3901 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     3961 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat
     4021 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     4081 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     4141 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     4201 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     4261 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     4321 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     4381 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     4441 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     4501 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     4561 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     4621 gtttgcgcaa cgttgttgcc attgctgcag gcatcgtggt gtcacgctcg tcgtttggta
     4681 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     4741 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     4801 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     4861 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     4921 gaccgagttg ctcttgcccg gcgtcaacac gggataatac cgcgccacat agcagaactt
     4981 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     5041 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     5101 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     5161 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     5221 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     5281 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta
     5341 ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt cttcaagaa
//
