LOCUS       40924_21005        6101 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Mammalian expression vector pGal4-p65f286-550, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6101)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6101)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 6101)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6101)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927, 
            9052, BELGIUM"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6101
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    complement(766..900)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    complement(934..1723)
                     /label=3' UTR of hNF-kappaB p65 subunit
                     /note="3' UTR of hNF-kappaB p65 subunit"
     CDS             complement(1727..2098)
                     /codon_start=1
                     /label=RelA (p65) AD
                     /note="transcriptional activation domain of human RelA,
                     also known as p65 (O'Shea and Perkins, 2008)"
                     /translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS
                     EFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGD
                     EDFSSIADMDFSALLSQISS"
     CDS             complement(2531..2971)
                     /codon_start=1
                     /label=GAL4 DNA binding domain
                     /note="DNA binding domain of the GAL4 transcriptional
                     activator"
                     /translation="LKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     promoter        complement(3113..3374)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     promoter        3699..4028
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      complement(4274..4862)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5036..5893)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(5894..5998)
                     /label=AmpR promoter
ORIGIN
        1 ttcctttgcc taatttaaat gaggacttaa cctgtggaaa tattttgatg tgggaagctg
       61 ttactgttaa aactgaggtt attggggtaa ctgctatgtt aaacttgcat tcagggacac
      121 aaaaaactca tgaaaatggt gctggaaaac ccattcaagg gtcaaatttt catttttttg
      181 ctgttggtgg ggaacctttg gagctgcagg gtgtgttagc aaactacagg accaaatatc
      241 ctgctcaaac tgtaacccca aaaaatgcta cagttgacag tcagcagatg aacactgacc
      301 acaaggctgt tttggataag gataatgctt atccagtgga gtgctgggtt cctgatccaa
      361 gtaaaaatga aaacactaga tattttggaa cctacacagg tggggaaaat gtgcctcctg
      421 ttttgcacat tactaacaca gcaaccacag tgcttcttga tgagcagggt gttgggccct
      481 tgtgcaaagc tgacagcttg tatgtttctg ctgttgacat ttgtgggctg tttaccaaca
      541 cttctggaac acagcagtgg aagggacttc ccagatattt taaaattacc cttagaaagc
      601 ggtctgtgaa aaacccctac ccaatttcct ttttgttaag tgacctaatt aacaggagga
      661 cacagagggt ggatgggcag cctatgattg gaatgtcctc tcaagtagag gaggttaggg
      721 tttatgagga cacagaggag cttcctgggg atcgctcgat cgagcgatcc agacatgata
      781 agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt
      841 tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt
      901 aattccgcca cggatcaagc ttgatatcga attccttttt tttttttttt cagcttggca
      961 acagctttat tagttcagag tagaaagagc aagagtccaa gtgctttgat tgttcactaa
     1021 aaactatgcc tcctgactgg agagctgcca gcctgctctc ccccactctt aacaacttac
     1081 cctactatta aggcacttga gaagagggga gagcaaggaa gtccagacca aaccccttct
     1141 ggatcctgga gagagccagt gctgttgcac tgtttccttc agccatggtt gagcaaggaa
     1201 agagccggca gagacctctg tagggcagga aggcagcccc tcaaacgctg gtgttaggca
     1261 cagggacaat gccagtgcca tacaggggct ggtatctggg gcgttatttt gattaagctg
     1321 taatgaatcc atgatggaag acacttgata aggctttgtg ggctcaaagg ccttacctcc
     1381 agcctgcttc tgtctctagg agagtaccag aagctggagg atggggatgg gggaccccac
     1441 agttccctac agagaaggga gctgaccatc aggacagggg aaaagtttga gtttccccag
     1501 ctcccccctt tccagagaag ttaatgcttc tgcttaagca cctccaaaaa gagagagaga
     1561 tacagatact gacaataaaa gaataaaata tggctccccc ctccaaggaa gacatccaca
     1621 aagttggggg cagttggaac acaccccacc agaatccgta agtgcttttg gagggcttca
     1681 atcccctgca accagtgctc tggggagggc aggcgtcacc cccttaggag ctgatctgac
     1741 tcagcagggc tgagaagtcc atgtccgcaa tggaggagaa gtcttcatct cctgaaagga
     1801 ggccattggg gagccccggg gcccccagtg gagcaggagc tgggtcgggg ggcctctggg
     1861 cccctgtcac taggcgagtt atagcctcag ggtactccat cagcatgggc tcagttgtgt
     1921 ggggggccac aggtatgccc tggttcagca gctgctgaaa ctcggagttg tcgacggatg
     1981 ccaggtctgt gaacacagct gggtctgtgc tgttgccaag caaggccccc aggtcttcat
     2041 catcaaactg cagctgcagc agggcctctg acagcgttcc ttccccagcc tgggtgggct
     2101 tgggggcagg tggggccaca gcctgaggag ggcctggggc taggactggg acaggggctg
     2161 gggcctgggc cagagctgat accatggctg gagcaggggc aggggctgga gcctggggca
     2221 ggacttgggg aggggccggg gccaaggccg aggcctggct gatctgccca gaaggaaaca
     2281 ccatggtggg aaactcatca tagttgatgg tgctcaggga tgacgtaaag ggatagggct
     2341 ggggtgctgg cttggggaca gaagctgagc tgcgggaagg cacagcaatg cgtcgaggtg
     2401 gaggccgggg gtcggtgggt ccgctgaaag gactcttctt catgatgctc ttgaaggtct
     2461 catatgtcct tttacgtttc tcctcaatcc ggtgacgatc gtctgtatct ggcaggtact
     2521 ggaattccgg cgatacagtc aactgtcttt gacctttgtt actactctct tccgatgatg
     2581 atgtcgcact tattctatgc tgtctcaatg ttagaggcat atcagtctcc actgaagcca
     2641 atctatctgt gacggcatct ttattcacat tatcttgtac aaataatcct gttaacaatg
     2701 cttttatatc ctgtaaagaa tccattttca aaatcatgtc aaggtcttct cgaggaaaaa
     2761 tcagtagaaa tagctgttcc agtctttcta gccttgattc cacttctgtc agatgtgccc
     2821 tagtcagcgg agaccttttg gttttgggag agtagcgaca ctcccagttg ttcttcagac
     2881 acttggcgca cttcggtttt tctttggagc acttgagctt tttaagtcgg caaatatcgc
     2941 atgcttgttc gatagaagac agtagcttca gatctggctg ctgggagtcc atcagtgaat
     3001 atcaactctg gcagaggagc cgctcgcccg ccaccgtccg cagttcccgc cgcagccgag
     3061 ataaacaact tagcttgtga acgcagaagg agcaggaggt acccagcttg gaggtgcaca
     3121 ccaatgtggt gaatggtcaa atggcgttta ttgtatcgag ctaggcactt aaatacaata
     3181 tctctgcaat gcggaattca gtggttcgtc caatccatgt cagacctgtc tgttgccttc
     3241 ctaataaggc acgatcgtac caccttactt ccaccaatcg gcatgcacgg tgctttttct
     3301 ctccttgtaa ggcatgttgc taactcatcg ttaccatgtt gcaagactac aagtgtattg
     3361 cataagacta catttccccc tccctatgca aaagcgaaac tactatatcc tgaggggact
     3421 cctaaccgcg tacaaccgaa gccccgcttt tcgcctaaac acaccctagt cccctcagat
     3481 acgcgtatat ctggcccgta catcgcgaag cagcgcaaaa cgcctaaccc taagcagatt
     3541 cttcatgcaa ttgtcggtca agccttgcct tgttgtagct taaattttgc tcgcgcacta
     3601 ctcagcgacc tccaacacac aagcagggag cagatactgg cttaactatg cggcatcaga
     3661 gcagattgta ctgagagtgc accatatggc tgtggaatgt gtgtcagtta gggtgtggaa
     3721 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
     3781 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     3841 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     3901 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     3961 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     4021 tttgcaaaaa gctccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc
     4081 gcatcaggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     4141 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     4201 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     4261 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     4321 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     4381 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     4441 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     4501 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     4561 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     4621 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     4681 tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc
     4741 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     4801 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     4861 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     4921 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     4981 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     5041 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     5101 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     5161 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     5221 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     5281 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     5341 ccattgctgc aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     5401 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     5461 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     5521 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     5581 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     5641 cggcgtcaac acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     5701 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     5761 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     5821 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     5881 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     5941 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     6001 catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct
     6061 ataaaaatag gcgtatcacg aggccctttc gtcttcaaga a
//
