LOCUS       40924_21000        5209 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Mammalian expression vector pGal4-p65f286-518, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5209)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5209)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 5209)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5209)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927, 
            9052, BELGIUM"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5209
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    complement(766..900)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(928..1635)
                     /codon_start=1
                     /note="unnamed protein product; p65f"
                     /protein_id="SJL87653.1"
                     /translation="EFQYLPDTDDRHRIEEKRKRTYETFKSIMKKSPFSGPTDPRPPPR
                     RIAVPSRSSASVPKPAPQPYPFTSSLSTINYDEFPTMVFPSGQISQASALAPAPPQVLP
                     QAPAPAPAPAMVSALAQAPAPVPVLAPGPPQAVAPPAPKPTQAGEGTLSEALLQLQFDD
                     EDLGALLGNSTDPAVFTDLASVDNSEFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTG
                     AQRPPDPAPAPLGA"
     CDS             complement(1639..2079)
                     /label=GAL4 DNA binding domain
                     /note="DNA binding domain of the GAL4 transcriptional
                     activator"
     promoter        complement(2221..2482)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     promoter        2807..3136
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      complement(3382..3970)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4144..5001)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(5002..5106)
                     /label=AmpR promoter
ORIGIN
        1 ttcctttgcc taatttaaat gaggacttaa cctgtggaaa tattttgatg tgggaagctg
       61 ttactgttaa aactgaggtt attggggtaa ctgctatgtt aaacttgcat tcagggacac
      121 aaaaaactca tgaaaatggt gctggaaaac ccattcaagg gtcaaatttt catttttttg
      181 ctgttggtgg ggaacctttg gagctgcagg gtgtgttagc aaactacagg accaaatatc
      241 ctgctcaaac tgtaacccca aaaaatgcta cagttgacag tcagcagatg aacactgacc
      301 acaaggctgt tttggataag gataatgctt atccagtgga gtgctgggtt cctgatccaa
      361 gtaaaaatga aaacactaga tattttggaa cctacacagg tggggaaaat gtgcctcctg
      421 ttttgcacat tactaacaca gcaaccacag tgcttcttga tgagcagggt gttgggccct
      481 tgtgcaaagc tgacagcttg tatgtttctg ctgttgacat ttgtgggctg tttaccaaca
      541 cttctggaac acagcagtgg aagggacttc ccagatattt taaaattacc cttagaaagc
      601 ggtctgtgaa aaacccctac ccaatttcct ttttgttaag tgacctaatt aacaggagga
      661 cacagagggt ggatgggcag cctatgattg gaatgtcctc tcaagtagag gaggttaggg
      721 tttatgagga cacagaggag cttcctgggg atcgctcgat cgagcgatcc agacatgata
      781 agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt
      841 tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt
      901 aattccgcca cggatcaagc ttgatggggc ccccagtgga gcaggagctg ggtcgggggg
      961 cctctgggcc cctgtcacta ggcgagttat agcctcaggg tactccatca gcatgggctc
     1021 agttgtgtgg ggggccacag gtatgccctg gttcagcagc tgctgaaact cggagttgtc
     1081 gacggatgcc aggtctgtga acacagctgg gtctgtgctg ttgccaagca aggcccccag
     1141 gtcttcatca tcaaactgca gctgcagcag ggcctctgac agcgttcctt ccccagcctg
     1201 ggtgggcttg ggggcaggtg gggccacagc ctgaggaggg cctggggcta ggactgggac
     1261 aggggctggg gcctgggcca gagctgatac catggctgga gcaggggcag gggctggagc
     1321 ctggggcagg acttggggag gggccggggc caaggccgag gcctggctga tctgcccaga
     1381 aggaaacacc atggtgggaa actcatcata gttgatggtg ctcagggatg acgtaaaggg
     1441 atagggctgg ggtgctggct tggggacaga agctgagctg cgggaaggca cagcaatgcg
     1501 tcgaggtgga ggccgggggt cggtgggtcc gctgaaagga ctcttcttca tgatgctctt
     1561 gaaggtctca tatgtccttt tacgtttctc ctcaatccgg tgacgatcgt ctgtatctgg
     1621 caggtactgg aattccggcg atacagtcaa ctgtctttga cctttgttac tactctcttc
     1681 cgatgatgat gtcgcactta ttctatgctg tctcaatgtt agaggcatat cagtctccac
     1741 tgaagccaat ctatctgtga cggcatcttt attcacatta tcttgtacaa ataatcctgt
     1801 taacaatgct tttatatcct gtaaagaatc cattttcaaa atcatgtcaa ggtcttctcg
     1861 aggaaaaatc agtagaaata gctgttccag tctttctagc cttgattcca cttctgtcag
     1921 atgtgcccta gtcagcggag accttttggt tttgggagag tagcgacact cccagttgtt
     1981 cttcagacac ttggcgcact tcggtttttc tttggagcac ttgagctttt taagtcggca
     2041 aatatcgcat gcttgttcga tagaagacag tagcttcaga tctggctgct gggagtccat
     2101 cagtgaatat caactctggc agaggagccg ctcgcccgcc accgtccgca gttcccgccg
     2161 cagccgagat aaacaactta gcttgtgaac gcagaaggag caggaggtac ccagcttgga
     2221 ggtgcacacc aatgtggtga atggtcaaat ggcgtttatt gtatcgagct aggcacttaa
     2281 atacaatatc tctgcaatgc ggaattcagt ggttcgtcca atccatgtca gacctgtctg
     2341 ttgccttcct aataaggcac gatcgtacca ccttacttcc accaatcggc atgcacggtg
     2401 ctttttctct ccttgtaagg catgttgcta actcatcgtt accatgttgc aagactacaa
     2461 gtgtattgca taagactaca tttccccctc cctatgcaaa agcgaaacta ctatatcctg
     2521 aggggactcc taaccgcgta caaccgaagc cccgcttttc gcctaaacac accctagtcc
     2581 cctcagatac gcgtatatct ggcccgtaca tcgcgaagca gcgcaaaacg cctaacccta
     2641 agcagattct tcatgcaatt gtcggtcaag ccttgccttg ttgtagctta aattttgctc
     2701 gcgcactact cagcgacctc caacacacaa gcagggagca gatactggct taactatgcg
     2761 gcatcagagc agattgtact gagagtgcac catatggctg tggaatgtgt gtcagttagg
     2821 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     2881 gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     2941 gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac
     3001 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
     3061 ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
     3121 cctaggcttt tgcaaaaagc tccatatgcg gtgtgaaata ccgcacagat gcgtaaggag
     3181 aaaataccgc atcaggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     3241 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     3301 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     3361 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     3421 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     3481 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     3541 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     3601 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     3661 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     3721 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     3781 agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg
     3841 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     3901 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     3961 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     4021 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     4081 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     4141 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     4201 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     4261 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     4321 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     4381 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     4441 cgttgttgcc attgctgcag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     4501 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     4561 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     4621 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     4681 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     4741 ctcttgcccg gcgtcaacac gggataatac cgcgccacat agcagaactt taaaagtgct
     4801 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     4861 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     4921 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     4981 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
     5041 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
     5101 tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac
     5161 attaacctat aaaaataggc gtatcacgag gccctttcgt cttcaagaa
//
