LOCUS       40924_20990        5393 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Mammalian expression vector pGal4-p65f1-285, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5393)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5393)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 5393)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5393)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927, 
            9052, BELGIUM"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5393
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    complement(766..900)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(929..1786)
                     /codon_start=1
                     /note="unnamed protein product; hNF-kappaB p65 subunit
                     (1-286)"
                     /protein_id="SJL87662.1"
                     /translation="MDELFPLIFPAEPAQASGPYVEIIEQPKQRGMRFRYKCEGRSAGS
                     IPGERSTDTTKTHPTIKINGYTGPGTVRISLVTKDPPHRPHPHELVGKDCRDGFYEAEL
                     CPDRCIHSFQNLGIQCVKKRDLEQAISQRIQTNNNPFQVPIEEQRGDYDLNAVRLCFQV
                     TVRDPSGRPLRLPPVLPHPIFDNRAPNTAELKICRVNRNSGSCLGGDEIFLLCDKVQKE
                     DIEVYFTGPGWEARGSFSQADVHRQVAIVFRTPPYADPSLQAPVRVSMQLRRPSDRELS
                     EPMEF"
     misc_feature    complement(1787..1822)
                     /label=linker
                     /note="linker"
     CDS             complement(1823..2263)
                     /label=GAL4 DNA binding domain
                     /note="DNA binding domain of the GAL4 transcriptional
                     activator"
     promoter        complement(2405..2666)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     promoter        2991..3320
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      complement(3566..4154)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4328..5185)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(5186..5290)
                     /label=AmpR promoter
ORIGIN
        1 ttcctttgcc taatttaaat gaggacttaa cctgtggaaa tattttgatg tgggaagctg
       61 ttactgttaa aactgaggtt attggggtaa ctgctatgtt aaacttgcat tcagggacac
      121 aaaaaactca tgaaaatggt gctggaaaac ccattcaagg gtcaaatttt catttttttg
      181 ctgttggtgg ggaacctttg gagctgcagg gtgtgttagc aaactacagg accaaatatc
      241 ctgctcaaac tgtaacccca aaaaatgcta cagttgacag tcagcagatg aacactgacc
      301 acaaggctgt tttggataag gataatgctt atccagtgga gtgctgggtt cctgatccaa
      361 gtaaaaatga aaacactaga tattttggaa cctacacagg tggggaaaat gtgcctcctg
      421 ttttgcacat tactaacaca gcaaccacag tgcttcttga tgagcagggt gttgggccct
      481 tgtgcaaagc tgacagcttg tatgtttctg ctgttgacat ttgtgggctg tttaccaaca
      541 cttctggaac acagcagtgg aagggacttc ccagatattt taaaattacc cttagaaagc
      601 ggtctgtgaa aaacccctac ccaatttcct ttttgttaag tgacctaatt aacaggagga
      661 cacagagggt ggatgggcag cctatgattg gaatgtcctc tcaagtagag gaggttaggg
      721 tttatgagga cacagaggag cttcctgggg atcgctcgat cgagcgatcc agacatgata
      781 agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt
      841 tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt
      901 aattccgcca cggatcaagc ttgatatcga attccatggg ctcactgagc tcccggtcgg
      961 aaggccgccg cagctgcatg gagacacgca caggagcctg caggctgggg tctgcgtagg
     1021 gaggggtccg gaacacaatg gccacttgtc ggtgcacatc agcttgcgaa aaggagcctc
     1081 gggcctccca gcctggtccc gtgaaataca cctcaatgtc ctctttctgc accttgtcac
     1141 acagtaggaa gatctcatcc ccaccgaggc agctgccaga gtttcggttc actcggcaga
     1201 tcttgagctc ggcagtgttg ggggcacgat tgtcaaagat gggatgagga aggacaggcg
     1261 gcaggcggag gggcctgcct gatgggtccc gcactgtcac ctggaagcag agccgcacag
     1321 cattcaggtc gtagtcccca cgctgctctt ctataggaac ttggaagggg ttgttgttgg
     1381 tctggatgcg ctgactgata gcctgctcca ggtcccgctt cttcacacac tggattccca
     1441 ggttctggaa actgtggatg cagcggtccg ggcagagctc agcctcatag aagccatccc
     1501 ggcagtcctt tcctacaagc tcgtgggggt gaggccggtg aggagggtcc ttggtgacca
     1561 gggagatgcg cactgtccct ggtcctgtgt agccattgat cttgatggtg gggtgggtct
     1621 tggtggtatc tgtgctcctc tcgcctggga tgctgcccgc ggagcgcccc tcgcacttgt
     1681 agcggaagcg catgccccgc tgcttgggct gctcaatgat ctccacatag gggccagagg
     1741 cctgggctgg ctctgccggg aagatgaggg ggaacagttc gtccatggcc ggggtcccct
     1801 ggtcgacacc cgggaattcc ggcgatacag tcaactgtct ttgacctttg ttactactct
     1861 cttccgatga tgatgtcgca cttattctat gctgtctcaa tgttagaggc atatcagtct
     1921 ccactgaagc caatctatct gtgacggcat ctttattcac attatcttgt acaaataatc
     1981 ctgttaacaa tgcttttata tcctgtaaag aatccatttt caaaatcatg tcaaggtctt
     2041 ctcgaggaaa aatcagtaga aatagctgtt ccagtctttc tagccttgat tccacttctg
     2101 tcagatgtgc cctagtcagc ggagaccttt tggttttggg agagtagcga cactcccagt
     2161 tgttcttcag acacttggcg cacttcggtt tttctttgga gcacttgagc tttttaagtc
     2221 ggcaaatatc gcatgcttgt tcgatagaag acagtagctt cagatctggc tgctgggagt
     2281 ccatcagtga atatcaactc tggcagagga gccgctcgcc cgccaccgtc cgcagttccc
     2341 gccgcagccg agataaacaa cttagcttgt gaacgcagaa ggagcaggag gtacccagct
     2401 tggaggtgca caccaatgtg gtgaatggtc aaatggcgtt tattgtatcg agctaggcac
     2461 ttaaatacaa tatctctgca atgcggaatt cagtggttcg tccaatccat gtcagacctg
     2521 tctgttgcct tcctaataag gcacgatcgt accaccttac ttccaccaat cggcatgcac
     2581 ggtgcttttt ctctccttgt aaggcatgtt gctaactcat cgttaccatg ttgcaagact
     2641 acaagtgtat tgcataagac tacatttccc cctccctatg caaaagcgaa actactatat
     2701 cctgagggga ctcctaaccg cgtacaaccg aagccccgct tttcgcctaa acacacccta
     2761 gtcccctcag atacgcgtat atctggcccg tacatcgcga agcagcgcaa aacgcctaac
     2821 cctaagcaga ttcttcatgc aattgtcggt caagccttgc cttgttgtag cttaaatttt
     2881 gctcgcgcac tactcagcga cctccaacac acaagcaggg agcagatact ggcttaacta
     2941 tgcggcatca gagcagattg tactgagagt gcaccatatg gctgtggaat gtgtgtcagt
     3001 tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     3061 attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
     3121 gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc
     3181 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
     3241 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg
     3301 gaggcctagg cttttgcaaa aagctccata tgcggtgtga aataccgcac agatgcgtaa
     3361 ggagaaaata ccgcatcagg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     3421 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     3481 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     3541 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     3601 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     3661 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     3721 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     3781 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     3841 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     3901 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     3961 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta
     4021 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     4081 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     4141 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     4201 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     4261 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     4321 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     4381 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     4441 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     4501 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     4561 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     4621 gcaacgttgt tgccattgct gcaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     4681 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     4741 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     4801 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     4861 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     4921 gttgctcttg cccggcgtca acacgggata ataccgcgcc acatagcaga actttaaaag
     4981 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     5041 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     5101 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     5161 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     5221 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     5281 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca
     5341 tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtcttcaa gaa
//
