LOCUS       40924_21030        6986 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Mammalian expression vector pGal4-p65S529A, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6986)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6986)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 6986)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6986)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927, 
            9052, BELGIUM"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6986
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    complement(766..900)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    complement(934..1723)
                     /label=3' UTR of hNF-kappaB p65 subunit
                     /note="3' UTR of hNF-kappaB p65 subunit"
     CDS             complement(1727..2098)
                     /codon_start=1
                     /label=RelA (p65) AD
                     /note="transcriptional activation domain of human RelA,
                     also known as p65 (O'Shea and Perkins, 2008)"
                     /translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS
                     EFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLAGD
                     EDFSSIADMDFSALLSQISS"
     misc_feature    complement(3380..3415)
                     /label=linker
                     /note="linker"
     CDS             complement(3416..3856)
                     /codon_start=1
                     /label=GAL4 DNA binding domain
                     /note="DNA binding domain of the GAL4 transcriptional
                     activator"
                     /translation="LKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     promoter        complement(3998..4259)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     promoter        4584..4913
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      complement(5159..5747)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5921..6778)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(6779..6883)
                     /label=AmpR promoter
ORIGIN
        1 ttcctttgcc taatttaaat gaggacttaa cctgtggaaa tattttgatg tgggaagctg
       61 ttactgttaa aactgaggtt attggggtaa ctgctatgtt aaacttgcat tcagggacac
      121 aaaaaactca tgaaaatggt gctggaaaac ccattcaagg gtcaaatttt catttttttg
      181 ctgttggtgg ggaacctttg gagctgcagg gtgtgttagc aaactacagg accaaatatc
      241 ctgctcaaac tgtaacccca aaaaatgcta cagttgacag tcagcagatg aacactgacc
      301 acaaggctgt tttggataag gataatgctt atccagtgga gtgctgggtt cctgatccaa
      361 gtaaaaatga aaacactaga tattttggaa cctacacagg tggggaaaat gtgcctcctg
      421 ttttgcacat tactaacaca gcaaccacag tgcttcttga tgagcagggt gttgggccct
      481 tgtgcaaagc tgacagcttg tatgtttctg ctgttgacat ttgtgggctg tttaccaaca
      541 cttctggaac acagcagtgg aagggacttc ccagatattt taaaattacc cttagaaagc
      601 ggtctgtgaa aaacccctac ccaatttcct ttttgttaag tgacctaatt aacaggagga
      661 cacagagggt ggatgggcag cctatgattg gaatgtcctc tcaagtagag gaggttaggg
      721 tttatgagga cacagaggag cttcctgggg atcgctcgat cgagcgatcc agacatgata
      781 agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt
      841 tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt
      901 aattccgcca cggatcaagc ttgatatcga attccttttt tttttttttt cagcttggca
      961 acagctttat tagttcagag tagaaagagc aagagtccaa gtgctttgat tgttcactaa
     1021 aaactatgcc tcctgactgg agagctgcca gcctgctctc ccccactctt aacaacttac
     1081 cctactatta aggcacttga gaagagggga gagcaaggaa gtccagacca aaccccttct
     1141 ggatcctgga gagagccagt gctgttgcac tgtttccttc agccatggtt gagcaaggaa
     1201 agagccggca gagacctctg tagggcagga aggcagcccc tcaaacgctg gtgttaggca
     1261 cagggacaat gccagtgcca tacaggggct ggtatctggg gcgttatttt gattaagctg
     1321 taatgaatcc atgatggaag acacttgata aggctttgtg ggctcaaagg ccttacctcc
     1381 agcctgcttc tgtctctagg agagtaccag aagctggagg atggggatgg gggaccccac
     1441 agttccctac agagaaggga gctgaccatc aggacagggg aaaagtttga gtttccccag
     1501 ctcccccctt tccagagaag ttaatgcttc tgcttaagca cctccaaaaa gagagagaga
     1561 tacagatact gacaataaaa gaataaaata tggctccccc ctccaaggaa gacatccaca
     1621 aagttggggg cagttggaac acaccccacc agaatccgta agtgcttttg gagggcttca
     1681 atcccctgca accagtgctc tggggagggc aggcgtcacc cccttaggag ctgatctgac
     1741 tcagcagggc tgagaagtcc atgtccgcaa tggaggagaa gtcttcatct cctgcaagga
     1801 gaccattggg gagccccggg gcccccagtg gagcaggagc tgggtcgggg ggcctctggg
     1861 cccctgtcac taggcgagtt atagcctcag ggtactccat cagcatgggc tcagttgtgt
     1921 ggggggccac aggtatgccc tggttcagca gctgctgaaa ctcggagttg tcgacggatg
     1981 ccaggtctgt gaacacagct gggtctgtgc tgttgccaag caaggccccc aggtcttcat
     2041 catcaaactg cagctgcagc agggcctctg acagcgttcc ttccccagcc tgggtgggct
     2101 tgggggcagg tggggccaca gcctgaggag ggcctggggc taggactggg acaggggctg
     2161 gggcctgggc cagagctgat accatggctg gagcaggggc aggggctgga gcctggggca
     2221 ggacttgggg aggggccggg gccaaggccg aggcctggct gatctgccca gaaggaaaca
     2281 ccatggtggg aaactcatca tagttgatgg tgctcaggga tgacgtaaag ggatagggct
     2341 ggggtgctgg cttggggaca gaagctgagc tgcgggaagg cacagcaatg cgtcgaggtg
     2401 gaggccgggg gtcggtgggt ccgctgaaag gactcttctt catgatgctc ttgaaggtct
     2461 catatgtcct tttacgtttc tcctcaatcc ggtgacgatc gtctgtatct ggcaggtact
     2521 ggaattccat gggctcactg agctcccggt cggaaggccg ccgcagctgc atggagacac
     2581 gcacaggagc ctgcaggctg gggtctgcgt agggaggggt ccggaacaca atggccactt
     2641 gtcggtgcac atcagcttgc gaaaaggagc ctcgggcctc ccagcctggt cccgtgaaat
     2701 acacctcaat gtcctctttc tgcaccttgt cacacagtag gaagatctca tccccaccga
     2761 ggcagctgcc agagtttcgg ttcactcggc agatcttgag ctcggcagtg ttgggggcac
     2821 gattgtcaaa gatgggatga ggaaggacag gcggcaggcg gaggggcctg cctgatgggt
     2881 cccgcactgt cacctggaag cagagccgca cagcattcag gtcgtagtcc ccacgctgct
     2941 cttctatagg aacttggaag gggttgttgt tggtctggat gcgctgactg atagcctgct
     3001 ccaggtcccg cttcttcaca cactggattc ccaggttctg gaaactgtgg atgcagcggt
     3061 ccgggcagag ctcagcctca tagaagccat cccggcagtc ctttcctaca agctcgtggg
     3121 ggtgaggccg gtgaggaggg tccttggtga ccagggagat gcgcactgtc cctggtcctg
     3181 tgtagccatt gatcttgatg gtggggtggg tcttggtggt atctgtgctc ctctcgcctg
     3241 ggatgctgcc cgcggagcgc ccctcgcact tgtagcggaa gcgcatgccc cgctgcttgg
     3301 gctgctcaat gatctccaca taggggccag aggcctgggc tggctctgcc gggaagatga
     3361 gggggaacag ttcgtccatg gccggggtcc cctggtcgac acccgggaat tccggcgata
     3421 cagtcaactg tctttgacct ttgttactac tctcttccga tgatgatgtc gcacttattc
     3481 tatgctgtct caatgttaga ggcatatcag tctccactga agccaatcta tctgtgacgg
     3541 catctttatt cacattatct tgtacaaata atcctgttaa caatgctttt atatcctgta
     3601 aagaatccat tttcaaaatc atgtcaaggt cttctcgagg aaaaatcagt agaaatagct
     3661 gttccagtct ttctagcctt gattccactt ctgtcagatg tgccctagtc agcggagacc
     3721 ttttggtttt gggagagtag cgacactccc agttgttctt cagacacttg gcgcacttcg
     3781 gtttttcttt ggagcacttg agctttttaa gtcggcaaat atcgcatgct tgttcgatag
     3841 aagacagtag cttcagatct ggctgctggg agtccatcag tgaatatcaa ctctggcaga
     3901 ggagccgctc gcccgccacc gtccgcagtt cccgccgcag ccgagataaa caacttagct
     3961 tgtgaacgca gaaggagcag gaggtaccca gcttggaggt gcacaccaat gtggtgaatg
     4021 gtcaaatggc gtttattgta tcgagctagg cacttaaata caatatctct gcaatgcgga
     4081 attcagtggt tcgtccaatc catgtcagac ctgtctgttg ccttcctaat aaggcacgat
     4141 cgtaccacct tacttccacc aatcggcatg cacggtgctt tttctctcct tgtaaggcat
     4201 gttgctaact catcgttacc atgttgcaag actacaagtg tattgcataa gactacattt
     4261 ccccctccct atgcaaaagc gaaactacta tatcctgagg ggactcctaa ccgcgtacaa
     4321 ccgaagcccc gcttttcgcc taaacacacc ctagtcccct cagatacgcg tatatctggc
     4381 ccgtacatcg cgaagcagcg caaaacgcct aaccctaagc agattcttca tgcaattgtc
     4441 ggtcaagcct tgccttgttg tagcttaaat tttgctcgcg cactactcag cgacctccaa
     4501 cacacaagca gggagcagat actggcttaa ctatgcggca tcagagcaga ttgtactgag
     4561 agtgcaccat atggctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc
     4621 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt
     4681 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
     4741 tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc
     4801 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg
     4861 agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc
     4921 atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgctctt
     4981 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     5041 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     5101 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     5161 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     5221 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     5281 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     5341 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     5401 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     5461 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     5521 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     5581 actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct
     5641 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     5701 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     5761 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     5821 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     5881 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     5941 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     6001 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     6061 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     6121 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     6181 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctgcaggca
     6241 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     6301 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     6361 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     6421 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     6481 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaacacggg
     6541 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     6601 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     6661 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     6721 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
     6781 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
     6841 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
     6901 tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta
     6961 tcacgaggcc ctttcgtctt caagaa
//
