LOCUS       40924_21040        4887 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Mammalian expression vector pGal4-VP16, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4887)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4887)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 4887)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4887)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927, 
            9052, BELGIUM"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4887
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    complement(766..900)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(1062..1295)
                     /codon_start=1
                     /label=VP16 AD
                     /note="transcriptional activation domain of herpes simplex
                     virus protein VP16 (Triezenberg et al., 1988; Cousens et 
                     al., 1989)"
                     /translation="APPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGF
                     TPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     misc_feature    complement(1296..1316)
                     /label=linker
                     /note="linker"
     CDS             complement(1317..1757)
                     /codon_start=1
                     /label=GAL4 DNA binding domain
                     /note="DNA binding domain of the GAL4 transcriptional
                     activator"
                     /translation="LKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     promoter        complement(1899..2160)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     promoter        2485..2814
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      complement(3060..3648)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3822..4679)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4680..4784)
                     /label=AmpR promoter
ORIGIN
        1 ttcctttgcc taatttaaat gaggacttaa cctgtggaaa tattttgatg tgggaagctg
       61 ttactgttaa aactgaggtt attggggtaa ctgctatgtt aaacttgcat tcagggacac
      121 aaaaaactca tgaaaatggt gctggaaaac ccattcaagg gtcaaatttt catttttttg
      181 ctgttggtgg ggaacctttg gagctgcagg gtgtgttagc aaactacagg accaaatatc
      241 ctgctcaaac tgtaacccca aaaaatgcta cagttgacag tcagcagatg aacactgacc
      301 acaaggctgt tttggataag gataatgctt atccagtgga gtgctgggtt cctgatccaa
      361 gtaaaaatga aaacactaga tattttggaa cctacacagg tggggaaaat gtgcctcctg
      421 ttttgcacat tactaacaca gcaaccacag tgcttcttga tgagcagggt gttgggccct
      481 tgtgcaaagc tgacagcttg tatgtttctg ctgttgacat ttgtgggctg tttaccaaca
      541 cttctggaac acagcagtgg aagggacttc ccagatattt taaaattacc cttagaaagc
      601 ggtctgtgaa aaacccctac ccaatttcct ttttgttaag tgacctaatt aacaggagga
      661 cacagagggt ggatgggcag cctatgattg gaatgtcctc tcaagtagag gaggttaggg
      721 tttatgagga cacagaggag cttcctgggg atcgctcgat cgagcgatcc agacatgata
      781 agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt
      841 tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt
      901 aattccgcca cggatcaagc ttcagctact taggatccac caaataatca gacgacaacc
      961 gcaggttttt gtaatgtatg tgctcgtgtt tattgtggat acgaaccggg gacgggaggg
     1021 gaaaacccag acgggggatg cgggtccggt cgcgccccct acccaccgta ctcgtcaatt
     1081 ccaagggcat cggtaaacat ctgctcaaac tcgaagtcgg ccatatccag agcgccgtag
     1141 ggggcggagt cgtggggggt aaatcccgga cccggggaat ccccgtcccc caacatgtcc
     1201 agatcgaaat cgtctagcgc gtcggcatgc gccatcgcca cgtcctcgcc gtctaagtgg
     1261 agctcgtccc ccaggctgac atcggtcggg ggggcccaga tccccgggaa ttccggcgat
     1321 acagtcaact gtctttgacc tttgttacta ctctcttccg atgatgatgt cgcacttatt
     1381 ctatgctgtc tcaatgttag aggcatatca gtctccactg aagccaatct atctgtgacg
     1441 gcatctttat tcacattatc ttgtacaaat aatcctgtta acaatgcttt tatatcctgt
     1501 aaagaatcca ttttcaaaat catgtcaagg tcttctcgag gaaaaatcag tagaaatagc
     1561 tgttccagtc tttctagcct tgattccact tctgtcagat gtgccctagt cagcggagac
     1621 cttttggttt tgggagagta gcgacactcc cagttgttct tcagacactt ggcgcacttc
     1681 ggtttttctt tggagcactt gagcttttta agtcggcaaa tatcgcatgc ttgttcgata
     1741 gaagacagta gcttcagatc tggctgctgg gagtccatca gtgaatatca actctggcag
     1801 aggagccgct cgcccgccac cgtccgcagt tcccgccgca gccgagataa acaacttagc
     1861 ttgtgaacgc agaaggagca ggaggtaccc agcttggagg tgcacaccaa tgtggtgaat
     1921 ggtcaaatgg cgtttattgt atcgagctag gcacttaaat acaatatctc tgcaatgcgg
     1981 aattcagtgg ttcgtccaat ccatgtcaga cctgtctgtt gccttcctaa taaggcacga
     2041 tcgtaccacc ttacttccac caatcggcat gcacggtgct ttttctctcc ttgtaaggca
     2101 tgttgctaac tcatcgttac catgttgcaa gactacaagt gtattgcata agactacatt
     2161 tccccctccc tatgcaaaag cgaaactact atatcctgag gggactccta accgcgtaca
     2221 accgaagccc cgcttttcgc ctaaacacac cctagtcccc tcagatacgc gtatatctgg
     2281 cccgtacatc gcgaagcagc gcaaaacgcc taaccctaag cagattcttc atgcaattgt
     2341 cggtcaagcc ttgccttgtt gtagcttaaa ttttgctcgc gcactactca gcgacctcca
     2401 acacacaagc agggagcaga tactggctta actatgcggc atcagagcag attgtactga
     2461 gagtgcacca tatggctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc
     2521 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag
     2581 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
     2641 atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
     2701 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct
     2761 gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc
     2821 catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgctct
     2881 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     2941 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     3001 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     3061 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     3121 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     3181 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     3241 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     3301 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     3361 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     3421 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     3481 aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc
     3541 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     3601 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     3661 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     3721 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     3781 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     3841 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     3901 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     3961 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     4021 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     4081 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctgcaggc
     4141 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     4201 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     4261 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
     4321 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
     4381 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaacacgg
     4441 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
     4501 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
     4561 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
     4621 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
     4681 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     4741 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
     4801 gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt
     4861 atcacgaggc cctttcgtct tcaagaa
//
