LOCUS       62056_11245        5398 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5398)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5398
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        27..330
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        331..534
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     promoter        623..641
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             672..1112
                     /codon_start=1
                     /label=GAL4 DNA binding domain
                     /note="DNA binding domain of the GAL4 transcriptional
                     activator"
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     intron          2083..2148
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             2278..2298
                     /codon_start=1
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /translation="PKKKRKV"
     polyA_signal    complement(2537..2671)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2782..2798)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2806..2822)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2830..2860)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2875..2896)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3184..3772)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3946..4803)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4804..4908)
                     /label=AmpR promoter
     primer_bind     5382..5398
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gagttcgagc ttgcatgcct gcaggtcgtt acataactta cggtaaatgg cccgcctggc
       61 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg
      121 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg
      181 gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa
      241 tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac
      301 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
      361 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
      421 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
      481 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta
      541 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac
      601 cgggaccgat ccagcctccg gataatacga ctcactatag gggttaagct tagacccaag
      661 gtaccgagtc catgaagcta ctgtcttcta tcgaacaagc atgcgatatt tgccgactta
      721 aaaagctcaa gtgctccaaa gaaaaaccga agtgcgccaa gtgtctgaag aacaactggg
      781 agtgtcgcta ctctcccaaa accaaaaggt ctccgctgac tagggcacat ctgacagaag
      841 tggaatcaag gctagaaaga ctggaacagc tatttctact gatttttcct cgagaagacc
      901 ttgacatgat tttgaaaatg gattctttac aggatataaa agcattgtta acaggattat
      961 ttgtacaaga taatgtgaat aaagatgccg tcacagatag attggcttca gtggagactg
     1021 atatgcctct aacattgaga cagcatagaa taagtgcgac atcatcatcg gaagagagta
     1081 gtaacaaagg tcaaagacag ttgactgtat cggacgagga atgggagctc atcaaaactg
     1141 tcaccgaagc ccatgtggcg accaacgccc aaggcagcca ctggaagcaa aaacggaaat
     1201 tcctgccaga agacattgga caagcaccaa tagtcaatgc cccagaaggt ggaaaggttg
     1261 acttggaagc cttcagccat tttacaaaaa tcatcacacc agcaattacc agagtggtgg
     1321 attttgccaa aaagttgcct atgttttgtg agctgccatg tgaagaccag atcatcctcc
     1381 tcaaaggctg ctgcatggag atcatgtccc ttcgcgctgc tgtgcgctat gacccagaaa
     1441 gtgagacttt aaccttgaat ggggaaatgg cagtgacacg gggccagctg aaaaatgggg
     1501 gtcttggggt ggtgtcagac gccatctttg acctgggcat gtctctgtct tctttcaacc
     1561 tggatgacac tgaagtagcc ctccttcagg ccgtcctgct gatgtcttca gatcgcccgg
     1621 ggcttgcctg tgttgagaga atagaaaagt accaagatag tttcctgctg gcctttgaac
     1681 actatatcaa ttaccgaaaa caccacgtga cacacttttg gccaaaactc ctgatgaagg
     1741 tgacagatct gcggatgata ggagcctgcc atgccagccg cttcctgcac atgaaggtgg
     1801 aatgccccac agaactcttc ccccctttgt tcttggaagt gtagtgtaac tgtattcagc
     1861 gatgacgaaa ttcttagcta ttgtaatact gcgatgagtg gcagggcggg gcgtaatttt
     1921 tttaaggcag ttattggtgc ccttaaacgc ctggttgcta cgcctgaata agtgataata
     1981 agcggatgaa tggcagaaat tcgccggatc tttgtgaagg aaccttactt ctgtggtgtg
     2041 acataattgg acaaactacc tacagagatt taaagctcta aggtaaatat aaaattttta
     2101 agtgtataat gtgttaaact actgattcta attgtttgtg tattttagat tccaacctat
     2161 ggaactgatg aatgggagca gtggtggaat gcctttaatg aggaaaacct gttttgctca
     2221 gaagaaatgc catctagtga tgatgaggct actgctgact ctcaacattc tactcctcca
     2281 aaaaagaaga gaaaggtaga agaccccaag gactttcctt cagaattgct aagttttttg
     2341 agtcatgctg tgtttagtaa tagaactctt gcttgctttg ctatttacac cacaaaggaa
     2401 aaagctgcac tgctatacaa gaaaattatg gaaaaatatt ctgtaacctt tataagtagg
     2461 cataacagtt ataatcataa catactgttt tttcttactc cacacaggca tagagtgtct
     2521 gcttagcggc cgcggggatc cagacatgat aagatacatt gatgagtttg gacaaaccac
     2581 aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt
     2641 tgtaaccatt ataagctgca ataaacaagt taacaacaac aattgcattc attttatgtt
     2701 tcaggttcag ggggaggtgt gggaggtttt ttcggatcct ctagagtcga tctgcaggca
     2761 tgctagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
     2821 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
     2881 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     2941 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     3001 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     3061 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     3121 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     3181 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     3241 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     3301 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     3361 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     3421 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     3481 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     3541 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     3601 gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt
     3661 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     3721 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     3781 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     3841 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     3901 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     3961 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     4021 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     4081 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     4141 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     4201 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     4261 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     4321 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     4381 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     4441 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     4501 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     4561 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     4621 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     4681 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     4741 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     4801 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     4861 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     4921 aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag
     4981 gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca
     5041 catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc
     5101 ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact atgcggcatc
     5161 agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag
     5221 gagaaaatac cgcatcaggc gccattcgcc attcaggctg cgcaactgtt gggaagggcg
     5281 atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg
     5341 attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagt
//
