LOCUS       V006122                 9175 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V006122
VERSION     V006122
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9175)
  AUTHORS   Dharmasena MN, Stark CEC., Stibitz S, Kopecko DJ.
  TITLE     Acid-resistant, attenuated microbial vector for improved oral
            delivery of multiple targeted antigens
  JOURNAL   Patent: US WO/2015/021147-B 12-FEB-2015; FDA-Center for Biologics
            Evaluation and Research; NIH campus Bldg. 29, 8800 Rockville Pike;
            Laboratory of Enteric and Sexually Transmitted Diseases; Bethesda,
            MD; USA;
REFERENCE   2  (bases 1 to 9175)
  AUTHORS   Dharmasena MN, Stark CEC., Stibitz S, Kopecko DJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAY-2014) Laboratory of Enteric and Sexually
            Transmitted Diseases, FDA-Center for Biologics Evaluation and
            Research, NIH Campus Bldg. 29, 8800 Rockville Pike, Bethesda, MD
            20892, USA
REFERENCE   3  (bases 1 to 9175)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9175)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Patent: US
            WO/2015/021147-B 12-FEB-2015; FDA-Center for Biologics Evaluation
            and Research; NIH campus Bldg. 29, 8800 Rockville Pike; Laboratory
            of Enteric and Sexually Transmitted Diseases; Bethesda, MD; USA;"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (16-MAY-2014) Laboratory of Enteric and Sexually Transmitted
            Diseases, FDA-Center for Biologics Evaluation and Research, NIH
            Campus Bldg. 29, 8800 Rockville Pike, Bethesda, MD 20892, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9175
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      480..1025
                     /label="p15A ori"
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells
                     that contain a second plasmid with the ColE1 origin."
     CDS             1620..2432
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
     CDS             complement(3053..3928)
                     /label="araC"
                     /note="L-arabinose regulatory protein"
     promoter        3955..4239
                     /label="araBAD promoter"
                     /note="promoter of the L-arabinose operon of E. coli; the
                     araC regulatory gene is transcribed in the opposite
                     direction (Guzman et al., 1995)"
     CDS             4267..5664
                     /gene="gadA"
                     /label="Glutamate decarboxylase alpha"
                     /note="Glutamate decarboxylase alpha from Escherichia coli
                     (strain K12). Accession#: P69908"
     CDS             5716..7113
                     /gene="gadB"
                     /label="Glutamate decarboxylase beta"
                     /note="Glutamate decarboxylase beta from Escherichia coli
                     (strain K12). Accession#: P69910"
     CDS             7138..8670
                     /gene="gadC"
                     /label="Glutamate/gamma-aminobutyrate antiporter"
                     /note="Glutamate/gamma-aminobutyrate antiporter from
                     Escherichia coli (strain K12). Accession#: P63235"
     terminator      8906..8992
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      9084..9111
                     /label="rrnB T2 terminator"
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
ORIGIN
        1 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
       61 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
      121 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
      181 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
      241 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
      301 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
      361 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
      421 atcccttaac gtgagttttc gttccactga gcgtcagacc ccttaataag atgatcttct
      481 tgagatcgtt ttggtctgcg cgtaatctct tgctctgaaa acgaaaaaac cgccttgcag
      541 ggcggttttt cgaaggttct ctgagctacc aactctttga accgaggtaa ctggcttgga
      601 ggagcgcagt caccaaaact tgtcctttca gtttagcctt aaccggcgca tgacttcaag
      661 actaactcct ctaaatcaat taccagtggc tgctgccagt ggtgcttttg catgtctttc
      721 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcggact gaacgggggg
      781 ttcgtgcata cagtccagct tggagcgaac tgcctacccg gaactgagtg tcaggcgtgg
      841 aatgagacaa acgcggccat aacagcggaa tgacaccggt aaaccgaaag gcaggaacag
      901 gagagcgcac gagggagccg ccagggggaa acgcctggta tctttatagt cctgtcgggt
      961 ttcgccacca ctgatttgag cgtcagattt cgtgatgctt gtcagggggg cggagcctat
     1021 ggaaaaacgg ctttgccgcg gccctctcac ttccctgtta agtatcttcc tggcatcttc
     1081 caggaaatct ccgccccgtt cgtaagccat ttccgctcgc cgcagtcgaa cgaccgagcg
     1141 tagcgagtca gtgagcgagg aagcggaata tatcctgtat cacatattct gctgacgcac
     1201 cggtgcagcc ttttttctcc tgccacatga agcacttcac tgacaccctc atcagtgcca
     1261 acatagtaag ccagtataca ctccgctagc gctgaggtct gcctcgtgaa gaaggtgttg
     1321 ctgactcata ccaggcctga atcgccccat catccagcca gaaagtgagg gagccacggt
     1381 tgatgagagc tttgttgtag gtggaccagt tggtgatttt gaacttttgc tttgccacgg
     1441 aacggtctgc gttgtcggga agatgcgtga tctgatcctt caactcagca aaagttcgat
     1501 ttattcaaca aagccacgtt gtgtctcaaa atctctgatg ttacattgca caagataaaa
     1561 atatatcatc atgaacaata aaactgtctg cttacataaa cagtaataca aggggtgtta
     1621 tgagccatat tcaacgggaa acgtcttgct cgaggccgcg attaaattcc aacatggatg
     1681 ctgatttata tgggtataaa tgggctcgcg ataatgtcgg gcaatcaggt gcgacaatct
     1741 atcgattgta tgggaagccc gatgcgccag agttgtttct gaaacatggc aaaggtagcg
     1801 ttgccaatga tgttacagat gagatggtca gactaaactg gctgacggaa tttatgcctc
     1861 ttccgaccat caagcatttt atccgtactc ctgatgatgc atggttactc accactgcga
     1921 tccccgggaa aacagcattc caggtattag aagaatatcc tgattcaggt gaaaatattg
     1981 ttgatgcgct ggcagtgttc ctgcgccggt tgcattcgat tcctgtttgt aattgtcctt
     2041 ttaacagcga tcgcgtattt cgtctcgctc aggcgcaatc acgaatgaat aacggtttgg
     2101 ttgatgcgag tgattttgat gacgagcgta atggctggcc tgttgaacaa gtctggaaag
     2161 aaatgcataa gcttttgcca ttctcaccgg attcagtcgt cactcatggt gatttctcac
     2221 ttgataacct tatttttgac gaggggaaat taataggttg tattgatgtt ggacgagtcg
     2281 gaatcgcaga ccgataccag gatcttgcca tcctatggaa ctgcctcggt gagttttctc
     2341 cttcattaca gaaacggctt tttcaaaaat atggtattga taatcctgat atgaataaat
     2401 tgcagtttca tttgatgctc gatgagtttt tctaatcaga attggttaat tggttgtaac
     2461 actggcagag cattacgctg acttgacggg acggcggctt tgttgaataa atcgaacttt
     2521 tgctgagttg aaggatcaga tcacgcatct tcccgacaac gcagaccgtt ccgtggcaaa
     2581 gcaaaagttc aaaatcacca actggtccac ctacaacaaa gctctcatca accgtggctc
     2641 cctcactttc tggctggatg atggggcgat tcaggcctgg tatgagtcag caacaccttc
     2701 ttcacgaggc agacctcagc gctcaaagat gcaggggtaa aagctaaccg catctttacc
     2761 gacaaggcat ccggcagttc aacagatcgg gaagggctgg atttgctgag gatgaaggtg
     2821 gaggaaggtg atgtcattct ggtgaagaag ctcgaccgtc ttggccgcga caccgccgac
     2881 atgatccaac tgataaaaga gtttgatgct cagggtgtag cggttcggtt tattgacgac
     2941 gggatcagta ccgacggtga tatggggcaa atggtggtca ccatcctgtc ggctgtggca
     3001 caggctgaac gccggaggat ctgccgtcaa ttgtctgatt cgttaccaat tatgacaact
     3061 tgacggctac atcattcact ttttcttcac aaccggcacg gaactcgctc gggctggccc
     3121 cggtgcattt tttaaatacc cgcgagaaat agagttgatc gtcaaaacca acattgcgac
     3181 cgacggtggc gataggcatc cgggtggtgc tcaaaagcag cttcgcctgg ctgatacgtt
     3241 ggtcctcgcg ccagcttaag acgctaatcc ctaactgctg gcggaaaaga tgtgacagac
     3301 gcgacggcga caagcaaaca tgctgtgcga cgctggcgat atcaaaattg ctgtctgcca
     3361 ggtgatcgct gatgtactga caagcctcgc gtacccgatt atccatcggt ggatggagcg
     3421 actcgttaat cgcttccatg cgccgcagta acaattgctc aagcagattt atcgccagca
     3481 gctccgaata gcgcccttcc ccttgcccgg cgttaatgat ttgcccaaac aggtcgctga
     3541 aatgcggctg gtgcgcttca tccgggcgaa agaaccccgt attggcaaat attgacggcc
     3601 agttaagcca ttcatgccag taggcgcgcg gacgaaagta aacccactgg tgataccatt
     3661 cgcgagcctc cggatgacga ccgtagtgat gaatctctcc tggcgggaac agcaaaatat
     3721 cacccggtcg gcaaacaaat tctcgtccct gatttttcac caccccctga ccgcgaatgg
     3781 tgagattgag aatataacct ttcattccca gcggtcggtc gataaaaaaa tcgagataac
     3841 cgttggcctc aatcggcgtt aaacccgcca ccagatgggc attaaacgag tatcccggca
     3901 gcaggggatc attttgcgct tcagccatac ttttcatact cccgccattc agagaagaaa
     3961 ccaattgtcc atattgcatc agacattgcc gtcactgcgt cttttactgg ctcttctcgc
     4021 taaccaaacc ggtaaccccg cttattaaaa gcattctgta acaaagcggg accaaagcca
     4081 tgacaaaaac gcgtaacaaa agtgtctata atcacggcag aaaagtccac attgattatt
     4141 tgcacggcgt cacactttgc tatgccatag catttttatc cataagatta gcggatccta
     4201 cctgacgctt tttatcgcaa ctctctactg tttctccata cccgtttttt tggatggagt
     4261 gaaacgatgg accagaagct gttaacggat ttccgctcag aactactcga ttcacgtttt
     4321 ggcgcaaagg ccatttctac tatcgcggag tcaaaacgat ttccgctgca cgaaatgcgc
     4381 gatgatgtcg cattccagat tatcaatgat gaattatatc ttgatggcaa cgctcgtcag
     4441 aacctagcca ctttctgcca gacctgggac gacgaaaacg tccataaatt gatggatttg
     4501 tcgatcaata aaaactggat cgacaaagaa gaatatccgc aatccgcagc catcgacctg
     4561 cgttgcgtaa atatggttgc cgatctgtgg catgcgcctg cgccgaaaaa tggtcaggcc
     4621 gttggcacca acaccattgg ttcttccgag gcctgtatgc tcggcgggat ggcgatgaaa
     4681 tggcgttggc gcaagcgtat ggaagctgca ggcaaaccaa cggataaacc aaacctagtg
     4741 tgcggtccgg tacaaatctg ctggcataaa ttcgcccgct actgggatgt ggagctgcgt
     4801 gagatcccta tgcgccccgg tcagttgttt atggacccga aacgcatgat tgaagcctgt
     4861 gacgaaaaca ccatcggcgt ggtgccgact ttcggcgtga cctacaccgg taactatgag
     4921 ttcccacaac cgctgcacga tgcgctggat aaattccagg ccgacaccgg tatcgacatc
     4981 gacatgcaca tcgacgctgc cagcggtggc ttcctggcac cgttcgtcgc cccggatatc
     5041 gtctgggact tccgcctgcc gcgtgtgaaa tcgatcagtg cttcaggcca taaattcggt
     5101 ctggctccgc tgggctgcgg ctgggttatc tggcgtgacg aagaagcgct gccgcaggaa
     5161 ctggtgttca acgttgacta cctgggtggt caaattggta cttttgccat caacttctcc
     5221 cgcccggcgg gtcaggtaat tgcacagtac tatgaattcc tgcgcctcgg tcgtgaaggc
     5281 tataccaaag tacagaacgc ctcttaccag gttgccgctt atctggcgga tgaaatcgcc
     5341 aaacaggggc cgtatgagtt catctgtacg ggtcgcccgg acgaaggcat cccggcggtt
     5401 tgcttcaaac tgaaagatgg tgaagatccg ggatacaccc tgtacgacct ctctgaacgt
     5461 ctgcgtctgc gcggctggca ggttccggcc ttcactctcg gcggtgaagc caccgacatc
     5521 gtggtgatgc gcattatgtg tcgtcgcggc ttcgaaatgg actttgctga actgttgctg
     5581 gaagactaca aagcctccct gaaatatctc agcgatcacc cgaaactgca gggtattgcc
     5641 cagcagaaca gctttaaaca cacctgacct acttggagtg ttttaatgcg atccaatcat
     5701 tttaaggagt ttaaaatgga taagaagcaa gtaacggatt taaggtcgga actactcgat
     5761 tcacgttttg gtgcgaagtc tatttccact atcgcagaat caaaacgttt tccgctgcac
     5821 gaaatgcgcg acgatgtcgc atttcagatt atcaatgatg aattatatct tgatggcaac
     5881 gctcgtcaga acctggccac tttctgccag acctgggacg acgaaaacgt ccacaagttg
     5941 atggatttat ccattaacaa aaactggatc gacaaagaag aatatccgca atccgcagcc
     6001 atcgacctgc gttgcgtaaa catggttgcc gatctgtggc atgcgcctgc gccgaaaaat
     6061 ggtcaggccg ttggcaccaa caccattggt tcttccgagg cctgtatgct cggcgggatg
     6121 gcgatgaaat ggcgttggcg caagcgtatg gaagctgcag gcaaaccaac ggataaacca
     6181 aacctggtgt gcggtccggt gcaaatctgc tggcataaat tcgcccgcta ctgggatgtg
     6241 gagctgcgtg agatccctat gcgccccggt cagttgttta tggacccgaa acgcatgatt
     6301 gaagcctgcg acgaaaatac catcggcgtg gtgccgactt tcggcgtgac ctacaccggt
     6361 aactatgagt tcccgcaacc gctgcacgat gcactggata aattccaggc cgacaccggt
     6421 atcgacatcg acatgcacat cgacgccgcc agcggtggct ttctggcacc gttcgtcgcc
     6481 ccggatatcg tctgggactt ccgcctgccg cgtgtgaaat cgatcagtgc ttcaggccat
     6541 aaattcggtc tggctccgct gggctgcggc tgggttatct ggcgtgacga agaagcgctg
     6601 ccgcaggaac tggtgttcaa cgttgactac ctcggtggtc agattggtac ttttgccatc
     6661 aacttctccc gcccggcggg tcaggtgatt gcacagtact atgaattcct gcgccttggt
     6721 cgtgaaggct ataccaaagt acagaatgct tcctaccagg tcgctgccta tctggcggat
     6781 gagatcgcca aactggggcc gtatgagttc atctgtaccg gtcgcccgga cgaaggcatc
     6841 ccggcggttt gcttcaaact gaaagatggt gaagatccgg gatacaccct gtacgacctc
     6901 tctgaacgtc tgcgtctgcg cggctggcag gttccggcct tcactctcgg cggtgaagcc
     6961 accgacatcg tggtgatgcg cattatgtgt cgtcgcggct tcgaaatgga ctttgctgaa
     7021 ctgttgctgg aagactacaa agcctccctg aaatatctca gcgatcaccc gaaactgcag
     7081 ggtattgccc agcagaacag ctttaaacat acctgataac cgttttggag tgataatatg
     7141 gctacatcag tacagacagg taaagctaag cagctcacat tactcggatt ctttgccata
     7201 acggcatcga tggtaatggc tgtttatgaa taccctacct tcgcaacatc gggcttttca
     7261 ttagtcttct tcctgctatt aggcgggatt ttatggttta ttcccgtggg actttgtgct
     7321 gcggaaatgg ccaccgtcga cggctgggaa gaaggtggtg tcttcgcctg ggtatcaaat
     7381 actctgggtc cgagatgggg atttgcagcg atctcatttg gctatctgca aatcgccatt
     7441 ggttttattc cgatgctcta tttcgtgtta ggggcactct cctacatcct gaaatggcca
     7501 gcgctgaatg aagaccccat taccaaaact attgcagcac tcatcattct ttgggcgctg
     7561 gcattaacgc agtttggtgg cacgaaatac acggcgcgaa ttgctaaagt tggcttcttc
     7621 gccggtatcc tgttacctgc atttattttg atcgcattag cggctattta cctgcactcc
     7681 ggtgcccccg ttgctatcga aatggattcg aagaccttct tccctgactt ctctaaagtg
     7741 ggcacactgg ttgtatttgt tgccttcatt ttgagttata tgggcgtaga agcttccgca
     7801 actcacgtca atgaaatgag caaccccggg cgcgactatc cactggctat gttactgctg
     7861 atggtggcgg caatctgctt aagctctgtt ggcggtttgt ctattgcgat ggtcattccg
     7921 ggtaatgaaa tcaacctctc cgcaggggta atgcaaacct ttaccgttct gatgtcccat
     7981 gtggcaccgg aaattgagtg gacggttcgc gtgatctccg cactgctgtt gctgggtgtt
     8041 ctggcggaaa tcgcctcctg gattgttggt ccttctcgcg ggatgtatgt cacagcgcag
     8101 aaaaacctgc tgccagcggc attcgctaaa atgaacaaaa atggcgtacc ggtaacgctg
     8161 gtcatttcgc agctggtgat tacttctatc gcgttgatca tcctcaccaa taccggtggc
     8221 ggtaacaaca tgtccttcct gatcgcactg gcgctgacgg tggtgattta tctgtgtgct
     8281 tatttcatgc tgtttattgg ctacattgtg ttggttctta aacatcctga cttaaaacgc
     8341 acatttaata tccctggtgg taaaggggtg aaactggtcg tggcaattgt cggtctgctg
     8401 acttcaatta tggcgtttat tgtttccttc ctgccgccgg ataacatcca gggtgattct
     8461 accgatatgt atgttgaatt actggttgtt agtttcctgg tggtacttgc cctgcccttt
     8521 attctctatg ctgttcatga tcgtaaaggc aaagcgaata ccggcgtcac tctggagcca
     8581 atcaacagtc agaacgcacc aaaaggtcac ttcttcctgc acccgcgtgc acgttcacca
     8641 cactatattg tgatgaatga caagaaacac taatctagag tcgacctgca ggcatgcaag
     8701 cttggctgtt ttggcggatg agagaagatt ttcagcctga tacagattaa atcagaacgc
     8761 agaagcggtc tgataaaaca gaatttgcct ggcggcagta gcgcggtggt cccacctgac
     8821 cccatgccga actcagaagt gaaacgccgt agcgccgatg gtagtgtggg gtctccccat
     8881 gcgagagtag ggaactgcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc
     8941 ctttcgtttt atctgttgtt tgtcggtgaa cgctctcctg agtaggacaa atccgccggg
     9001 agcggatttg aacgttgcga agcaacggcc cggagggtgg cgggcaggac gcccgccata
     9061 aactgccagg catcaaatta agcagaaggc catcctgacg gatggccttt ttgcgtttct
     9121 acaaactctt ttgtttattt ttctaaatac attcaaatat gtatccgctc atgca
//
