LOCUS       40924_23999       11138 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Ti plasmid binary vector pGZ14.1021, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11138)
  AUTHORS   Zhang Y, Guiltinan MJ, Maximova SN.
  TITLE     Characterization of stearoyl-acyl carrier protein-desaturase gene 
            family from chocolate tree, Theobroma cacao L.
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11138)
  AUTHORS   Zhang Y, Guiltinan MJ, Maximova SN.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2014) Plant Sciences, Penn State University, 422 
            Life Sciences Bldg, University Park, PA 16802, USA
REFERENCE   3  (bases 1 to 11138)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 11138)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (05-NOV-2014) Plant Sciences, Penn State University, 422 Life 
            Sciences Bldg, University Park, PA 16802, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11138
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    complement(10..184)
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(210..1400)
                     /codon_start=1
                     /gene="TcSAD1"
                     /product="stearoyl-acyl carrier protein-desaturase 1"
                     /label=TcSAD1
                     /protein_id="AJP67512.1"
                     /translation="MALKLNPITSQSQKLPYFALPPMASLRSPKFFMASTLRSGSKEVE
                     NVKKPFMPPREVHVQVTHSMPPQKIEIFKSLENWAEQNILVHLKPVEKCWQPQDFLPDP
                     ASDGFDEQVKELRERAKEIPDDYFVVLVGDMITEEALPTYQTMLNTLDGVLDETGASLT
                     SWAIWTRAWTAEENRHGDLLNKYLYLSGRVDMRQIEKTIQYLIGSGMDPRTENSPYLGF
                     IYTSFQERATFISHGNTARLAKEHGDFKLAQICGTIASDERRHETAYTKIVEKLFEIDP
                     DGTVLAFADMMRKKISMPAHLMYDGRDDNLFDHFSAVAQRLGVYTAKDYADILEFLVER
                     WKVKELTGLSADGRKAQDYVCGLPPRIRRLEERAQGRAKQAPSIPFSWIFDREVKL"
     gene            complement(210..1400)
                     /gene="TcSAD1"
                     /label=TcSAD1
     promoter        complement(1481..1823)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(2240..2256)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    2459..2483
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             3783..4409
                     /codon_start=1
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             4846..5910
                     /codon_start=1
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     rep_origin      5979..6173
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     misc_feature    6517..6657
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(6843..7431)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(7521..8312)
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    8737..8761
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    complement(8839..9013)
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(9056..10078)
                     /codon_start=1
                     /label=HygR
                     /note="aminoglycoside phosphotransferase from E. coli"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     promoter        complement(10146..10823)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     protein_bind    11014..11035
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        11050..11080
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11088..11104
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11112..11128
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 aattcggggg atctggattt tagtactgga ttttggtttt aggaattaga aattttattg
       61 atagaagtat tttacaaata caaatacata ctaagggttt cttatatgct caacacatga
      121 gcgaaaccct ataggaaccc taattccctt atctgggaac tactcacaca ttattatgga
      181 gaaactcgag cttgcatgcc aattctagac tagagcttca cttctctatc aaaaatccaa
      241 ctgaacggaa tactgggtgc ttgcttggcc ctcccttgag ccctctcctc tagccttctg
      301 attcttggag gaagtccaca cacataatct tgagctttac gcccatccgc tgaaagtcca
      361 gtcagctcct tcaccttcca tctctccacc aggaactcca gaatatcagc gtagtccttg
      421 gcagtgtaaa ccccaagtct ttgtgcaaca gctgagaagt ggtcgaaaag gttgtcatct
      481 cggccatcat acatcaagtg ggcaggcatc gagattttct ttctcatcat gtcagcaaag
      541 gccaggacag taccatcagg atcaatctca aagagctttt ccacgatttt agtataagct
      601 gtctcatggc gcctttcatc cgaggcaatc gtcccacata tttgagccaa cttaaagtcc
      661 ccatgctcct tggcaagtct ggctgtattc ccatgggaga taaaagttgc cctttcttgg
      721 aatgatgtgt agatgaatcc aagataaggg ctgttttctg tgcggggatc cattcctgat
      781 ccaatcaaat actggattgt cttctcaatt tgtctcatgt ccactcttcc agacaagtag
      841 aggtacttat taagtagatc accatgccta ttttcttcag ctgtccatgc cctcgtccaa
      901 attgcccaag aggtgaggct agcacccgtt tcatcaagaa ctccatctaa ggtattaagc
      961 attgtttggt aagttggaag ggcttcctct gtgatcatat caccaaccaa aacaacaaag
     1021 taatcatctg gaatctcctt agccctttcc cttagttctt ttacttgctc atcaaatcca
     1081 tcagaagcag gatctggaag aaaatcctgg ggttgccaac atttctcaac tggtttgagg
     1141 tgaactaaaa tgttctgctc agcccagttc tccaaagatt taaagatctc aatcttttgt
     1201 ggtggcatgg agtgggtgac ctgaacatga acctcccgag gaggcatgaa aggctttttg
     1261 acattctcaa cctctttgga gccagaacga agagtggagg ccatgaaaaa cttgggagat
     1321 ctgaggctgg ccattggtgg aagagcaaaa tacggaagtt tctgagattg agaagtgatg
     1381 ggattcaatt tcagagccat tctagatgta gttgtagaat gtaaaatgta atgttgttgt
     1441 tgtttgttgt tgttgttggt aattgttgta aaaatacgta cctctccgaa tgaaatgaac
     1501 ttccttatat agaggaaggg tcttgcgaag gatagtggga ttgtgcgtca tcccttacgt
     1561 cagtggagat atcacatcaa tccacttgct ttgaagacgt ggttggaacg tcttcttttt
     1621 ccacgatgct cctcgtgggt gggggtccat ctttgggacc actgtcggca gaggcatctt
     1681 gaacgatagc ctttccttta tcgcaatgat ggcatttgta ggtgccacct tccttttcta
     1741 ctgtcctttt gatgaagtga cagatagctg ggcaatggaa tccgaggagg tttcccgata
     1801 ttaccctttg ttgaaaagtc tcaatagccc tttggtcttc tgagactgta tctttgatat
     1861 tcttggagta gacgagagtg tcgtgctcca ccatgttcac atcaatccac ttgctttgaa
     1921 gacgtggttg gaacgtcttc tttttccacg atgctcctcg tgggtggggg tccatctttg
     1981 ggaccactgt cggcagaggc atcttgaacg atagcctttc ctttatcgca atgatggcat
     2041 ttgtaggtgc caccttcctt ttctactgtc cttttgatga agtgacagat agctgggcaa
     2101 tggaatccga ggaggtttcc cgatattacc ctttgttgaa aagtctcaat agccctttgg
     2161 tcttctgaga ctgtatcttt gatattcttg gagtagacga gagtgtcgtg ctccaccatg
     2221 ttggcaagct aagcttggca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg
     2281 gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg
     2341 aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatgctaga
     2401 gcagcttgag cttggatcag attgtcgttt cccgccttca gtttaaacta tcagtgtttg
     2461 acaggatata ttggcgggta aacctaagag aaaagagcgt ttattagaat aacggatatt
     2521 taaaagggcg tgaaaaggtt tatccgttcg tccatttgta tgtgcatgcc aaccacaggg
     2581 ttcccctcgg gatcaaagta ctttgatcca acccctccgc tgctatagtg cagtcggctt
     2641 ctgacgttca gtgcagccgt cttctgaaaa cgacatgtcg cacaagtcct aagttacgcg
     2701 acaggctgcc gccctgccct tttcctggcg ttttcttgtc gcgtgtttta gtcgcataaa
     2761 gtagaatact tgcgactaga accggagaca ttacgccatg aacaagagcg ccgccgctgg
     2821 cctgctgggc tatgcccgcg tcagcaccga cgaccaggac ttgaccaacc aacgggccga
     2881 actgcacgcg gccggctgca ccaagctgtt ttccgagaag atcaccggca ccaggcgcga
     2941 ccgcccggag ctggccagga tgcttgacca cctacgccct ggcgacgttg tgacagtgac
     3001 caggctagac cgcctggccc gcagcacccg cgacctactg gacattgccg agcgcatcca
     3061 ggaggccggc gcgggcctgc gtagcctggc agagccgtgg gccgacacca ccacgccggc
     3121 cggccgcatg gtgttgaccg tgttcgccgg cattgccgag ttcgagcgtt ccctaatcat
     3181 cgaccgcacc cggagcgggc gcgaggccgc caaggcccga ggcgtgaagt ttggcccccg
     3241 ccctaccctc accccggcac agatcgcgca cgcccgcgag ctgatcgacc aggaaggccg
     3301 caccgtgaaa gaggcggctg cactgcttgg cgtgcatcgc tcgaccctgt accgcgcact
     3361 tgagcgcagc gaggaagtga cgcccaccga ggccaggcgg cgcggtgcct tccgtgagga
     3421 cgcattgacc gaggccgacg ccctggcggc cgccgagaat gaacgccaag aggaacaagc
     3481 atgaaaccgc accaggacgg ccaggacgaa ccgtttttca ttaccgaaga gatcgaggcg
     3541 gagatgatcg cggccgggta cgtgttcgag ccgcccgcgc acgtctcaac cgtgcggctg
     3601 catgaaatcc tggccggttt gtctgatgcc aagctggcgg cctggccggc cagcttggcc
     3661 gctgaagaaa ccgagcgccg ccgtctaaaa aggtgatgtg tatttgagta aaacagcttg
     3721 cgtcatgcgg tcgctgcgta tatgatgcga tgagtaaata aacaaatacg caaggggaac
     3781 gcatgaaggt tatcgctgta cttaaccaga aaggcgggtc aggcaagacg accatcgcaa
     3841 cccatctagc ccgcgccctg caactcgccg gggccgatgt tctgttagtc gattccgatc
     3901 cccagggcag tgcccgcgat tgggcggccg tgcgggaaga tcaaccgcta accgttgtcg
     3961 gcatcgaccg cccgacgatt gaccgcgacg tgaaggccat cggccggcgc gacttcgtag
     4021 tgatcgacgg agcgccccag gcggcggact tggctgtgtc cgcgatcaag gcagccgact
     4081 tcgtgctgat tccggtgcag ccaagccctt acgacatatg ggccaccgcc gacctggtgg
     4141 agctggttaa gcagcgcatt gaggtcacgg atggaaggct acaagcggcc tttgtcgtgt
     4201 cgcgggcgat caaaggcacg cgcatcggcg gtgaggttgc cgaggcgctg gccgggtacg
     4261 agctgcccat tcttgagtcc cgtatcacgc agcgcgtgag ctacccaggc actgccgccg
     4321 ccggcacaac cgttcttgaa tcagaacccg agggcgacgc tgcccgcgag gtccaggcgc
     4381 tggccgctga aattaaatca aaactcattt gagttaatga ggtaaagaga aaatgagcaa
     4441 aagcacaaac acgctaagtg ccggccgtcc gagcgcacgc agcagcaagg ctgcaacgtt
     4501 ggccagcctg gcagacacgc cagccatgaa gcgggtcaac tttcagttgc cggcggagga
     4561 tcacaccaag ctgaagatgt acgcggtacg ccaaggcaag accattaccg agctgctatc
     4621 tgaatacatc gcgcagctac cagagtaaat gagcaaatga ataaatgagt agatgaattt
     4681 tagcggctaa aggaggcggc atggaaaatc aagaacaacc aggcaccgac gccgtggaat
     4741 gccccatgtg tggaggaacg ggcggttggc caggcgtaag cggctgggtt gtctgccggc
     4801 cctgcaatgg cactggaacc cccaagcccg aggaatcggc gtgacggtcg caaaccatcc
     4861 ggcccggtac aaatcggcgc ggcgctgggt gatgacctgg tggagaagtt gaaggccgcg
     4921 caggccgccc agcggcaacg catcgaggca gaagcacgcc ccggtgaatc gtggcaagcg
     4981 gccgctgatc gaatccgcaa agaatcccgg caaccgccgg cagccggtgc gccgtcgatt
     5041 aggaagccgc ccaagggcga cgagcaacca gattttttcg ttccgatgct ctatgacgtg
     5101 ggcacccgcg atagtcgcag catcatggac gtggccgttt tccgtctgtc gaagcgtgac
     5161 cgacgagctg gcgaggtgat ccgctacgag cttccagacg ggcacgtaga ggtttccgca
     5221 gggccggccg gcatggccag tgtgtgggat tacgacctgg tactgatggc ggtttcccat
     5281 ctaaccgaat ccatgaaccg ataccgggaa gggaagggag acaagcccgg ccgcgtgttc
     5341 cgtccacacg ttgcggacgt actcaagttc tgccggcgag ccgatggcgg aaagcagaaa
     5401 gacgacctgg tagaaacctg cattcggtta aacaccacgc acgttgccat gcagcgtacg
     5461 aagaaggcca agaacggccg cctggtgacg gtatccgagg gtgaagcctt gattagccgc
     5521 tacaagatcg taaagagcga aaccgggcgg ccggagtaca tcgagatcga gctagctgat
     5581 tggatgtacc gcgagatcac agaaggcaag aacccggacg tgctgacggt tcaccccgat
     5641 tactttttga tcgatcccgg catcggccgt tttctctacc gcctggcacg ccgcgccgca
     5701 ggcaaggcag aagccagatg gttgttcaag acgatctacg aacgcagtgg cagcgccgga
     5761 gagttcaaga agttctgttt caccgtgcgc aagctgatcg ggtcaaatga cctgccggag
     5821 tacgatttga aggaggaggc ggggcaggct ggcccgatcc tagtcatgcg ctaccgcaac
     5881 ctgatcgagg gcgaagcatc cgccggttcc taatgtacgg agcagatgct agggcaaatt
     5941 gccctagcag gggaaaaagg tcgaaaaggt ctctttcctg tggatagcac gtacattggg
     6001 aacccaaagc cgtacattgg gaaccggaac ccgtacattg ggaacccaaa gccgtacatt
     6061 gggaaccggt cacacatgta agtgactgat ataaaagaga aaaaaggcga tttttccgcc
     6121 taaaactctt taaaacttat taaaactctt aaaacccgcc tggcctgtgc ataactgtct
     6181 ggccagcgca cagccgaaga gctgcaaaaa gcgcctaccc ttcggtcgct gcgctcccta
     6241 cgccccgccg cttcgcgtcg gcctatcgcg gccgctggcc gctcaaaaat ggctggccta
     6301 cggccaggca atctaccagg gcgcggacaa gccgcgccgt cgccactcga ccgccggcgc
     6361 ccacatcaag gcaccctgcc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat
     6421 gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg
     6481 tcagggcgcg tcagcgggtg ttggcgggtg tcggggcgca gccatgaccc agtcacgtag
     6541 cgatagcgga gtgtatactg gcttaactat gcggcatcag agcagattgt actgagagtg
     6601 caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgc
     6661 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     6721 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     6781 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     6841 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     6901 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     6961 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     7021 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     7081 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     7141 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     7201 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     7261 cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt
     7321 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     7381 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     7441 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     7501 gtcatgcatt ctaggtacta aaacaattca tccagtaaaa tataatattt tattttctcc
     7561 caatcaggct tgatccccag taagtcaaaa aatagctcga catactgttc ttccccgata
     7621 tcctccctga tcgaccggac gcagaaggca atgtcatacc acttgtccgc cctgccgctt
     7681 ctcccaagat caataaagcc acttactttg ccatctttca caaagatgtt gctgtctccc
     7741 aggtcgccgt gggaaaagac aagttcctct tcgggctttt ccgtctttaa aaaatcatac
     7801 agctcgcgcg gatctttaaa tggagtgtct tcttcccagt tttcgcaatc cacatcggcc
     7861 agatcgttat tcagtaagta atccaattcg gctaagcggc tgtctaagct attcgtatag
     7921 ggacaatccg atatgtcgat ggagtgaaag agcctgatgc actccgcata cagctcgata
     7981 atcttttcag ggctttgttc atcttcatac tcttccgagc aaaggacgcc atcggcctca
     8041 ctcatgagca gattgctcca gccatcatgc cgttcaaagt gcaggacctt tggaacaggc
     8101 agctttcctt ccagccatag catcatgtcc ttttcccgtt ccacatcata ggtggtccct
     8161 ttataccggc tgtccgtcat ttttaaatat aggttttcat tttctcccac cagcttatat
     8221 accttagcag gagacattcc ttccgtatct tttacgcagc ggtatttttc gatcagtttt
     8281 ttcaattccg gtgatattct cattttagcc atttattatt tccttcctct tttctacagt
     8341 atttaaagat accccaagaa gctaattata acaagacgaa ctccaattca ctgttccttg
     8401 cattctaaaa ccttaaatac cagaaaacag ctttttcaaa gttgttttca aagttggcgt
     8461 ataacatagt atcgacggag ccgattttga aaccgcggtg atcacaggca gcaacgctct
     8521 gtcatcgtta caatcaacat gctaccctcc gcgagatcat ccgtgtttca aacccggcag
     8581 cttagttgcc gttcttccga atagcatcgg taacatgagc aaagtctgcc gccttacaac
     8641 ggctctcccg ctgacgccgt cccggactga tgggctgcct gtatcgagtg gtgattttgt
     8701 gccgagctgc cggtcgggga gctgttggct ggctggtggc aggatatatt gtggtgtaaa
     8761 caaattgacg cttagacaac ttaataacac attgcggacg tttttaatgt actgaattaa
     8821 cgccgaatta attcggggga tctggatttt agtactggat tttggtttta ggaattagaa
     8881 attttattga tagaagtatt ttacaaatac aaatacatac taagggtttc ttatatgctc
     8941 aacacatgag cgaaacccta taggaaccct aattccctta tctgggaact actcacacat
     9001 tattatggag aaactcgagc ttgtcgatcg acagatccgg tcggcatcta ctctatttct
     9061 ttgccctcgg acgagtgctg gggcgtcggt ttccactatc ggcgagtact tctacacagc
     9121 catcggtcca gacggccgcg cttctgcggg cgatttgtgt acgcccgaca gtcccggctc
     9181 cggatcggac gattgcgtcg catcgaccct gcgcccaagc tgcatcatcg aaattgccgt
     9241 caaccaagct ctgatagagt tggtcaagac caatgcggag catatacgcc cggagtcgtg
     9301 gcgatcctgc aagctccgga tgcctccgct cgaagtagcg cgtctgctgc tccatacaag
     9361 ccaaccacgg cctccagaag aagatgttgg cgacctcgta ttgggaatcc ccgaacatcg
     9421 cctcgctcca gtcaatgacc gctgttatgc ggccattgtc cgtcaggaca ttgttggagc
     9481 cgaaatccgc gtgcacgagg tgccggactt cggggcagtc ctcggcccaa agcatcagct
     9541 catcgagagc ctgcgcgacg gacgcactga cggtgtcgtc catcacagtt tgccagtgat
     9601 acacatgggg atcagcaatc gcgcatatga aatcacgcca tgtagtgtat tgaccgattc
     9661 cttgcggtcc gaatgggccg aacccgctcg tctggctaag atcggccgca gcgatcgcat
     9721 ccatagcctc cgcgaccggt tgtagaacag cgggcagttc ggtttcaggc aggtcttgca
     9781 acgtgacacc ctgtgcacgg cgggagatgc aataggtcag gctctcgcta aactccccaa
     9841 tgtcaagcac ttccggaatc gggagcgcgg ccgatgcaaa gtgccgataa acataacgat
     9901 ctttgtagaa accatcggcg cagctattta cccgcaggac atatccacgc cctcctacat
     9961 cgaagctgaa agcacgagat tcttcgccct ccgagagctg catcaggtcg gagacgctgt
    10021 cgaacttttc gatcagaaac ttctcgacag acgtcgcggt gagttcaggc tttttcatat
    10081 ctcattgccc cccgggatct gcgaaagctc gagagagata gatttgtaga gagagactgg
    10141 tgatttcagc gtgtcctctc caaatgaaat gaacttcctt atatagagga aggtcttgcg
    10201 aaggatagtg ggattgtgcg tcatccctta cgtcagtgga gatatcacat caatccactt
    10261 gctttgaaga cgtggttgga acgtcttctt tttccacgat gctcctcgtg ggtgggggtc
    10321 catctttggg accactgtcg gcagaggcat cttgaacgat agcctttcct ttatcgcaat
    10381 gatggcattt gtaggtgcca ccttcctttt ctactgtcct tttgatgaag tgacagatag
    10441 ctgggcaatg gaatccgagg aggtttcccg atattaccct ttgttgaaaa gtctcaatag
    10501 ccctttggtc ttctgagact gtatctttga tattcttgga gtagacgaga gtgtcgtgct
    10561 ccaccatgtt atcacatcaa tccacttgct ttgaagacgt ggttggaacg tcttcttttt
    10621 ccacgatgct cctcgtgggt gggggtccat ctttgggacc actgtcggca gaggcatctt
    10681 gaacgatagc ctttccttta tcgcaatgat ggcatttgta ggtgccacct tccttttcta
    10741 ctgtcctttt gatgaagtga cagatagctg ggcaatggaa tccgaggagg tttcccgata
    10801 ttaccctttg ttgaaaagtc tcaatagccc tttggtcttc tgagactgta tctttgatat
    10861 tcttggagta gacgagagtg tcgtgctcca ccatgttggc aagctgctct agccaatacg
    10921 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc
    10981 cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc
    11041 accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata
    11101 acaatttcac acaggaaaca gctatgacca tgattacg
//
