LOCUS       V013048                 7262 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V013048
VERSION     V013048
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7262)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..7262
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(10..26)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        500..604
                     /label="AmpR promoter"
     CDS             605..1462
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      1636..2224
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    2512..2533
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2548..2578
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    2586..2602
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2610..2626
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     rep_origin      complement(2664..2799)
                     /direction=LEFT
                     /label="SV40 ori"
                     /note="SV40 origin of replication"
     polyA_signal    complement(2805..2939)
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     LTR             complement(3011..3244)
                     /label="3' LTR (Delta-U3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     misc_feature    complement(3335..3923)
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     CDS             complement(3937..4308)
                     /label="BleoR"
                     /note="antibiotic-binding protein"
     CDS             complement(4315..4368)
                     /label="T2A"
                     /note="2A peptide from Thosea asigna virus capsid protein"
     CDS             complement(4510..5175)
                     /label="CopGFP"
                     /note="green fluorescent protein 2 from Pontellina plumata,
                     also known as ppluGFP2 (Shagin et al., 2004)"
     misc_feature    complement(5343..5459)
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     CDS             complement(5536..5577)
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     CDS             complement(5726..5770)
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     misc_feature    complement(5954..6187)
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     misc_feature    complement(6680..6805)
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     promoter        complement(7030..7257)
                     /label="RSV promoter"
                     /note="Rous sarcoma virus enhancer/promoter"
ORIGIN
        1 cagcttggca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca
       61 acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg
      121 caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatggcgcc tgatgcggta
      181 ttttctcctt acgcatctgt gcggtatttc acaccgcata tggtgcactc tcagtacaat
      241 ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg ctgacgcgcc
      301 ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag
      361 ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgagacgaa agggcctcgt
      421 gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg
      481 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
      541 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
      601 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
      661 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
      721 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
      781 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
      841 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
      901 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
      961 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     1021 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     1081 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     1141 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     1201 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     1261 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     1321 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     1381 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     1441 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     1501 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     1561 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     1621 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     1681 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     1741 gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta
     1801 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     1861 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     1921 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     1981 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     2041 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     2101 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     2161 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     2221 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     2281 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     2341 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     2401 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
     2461 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
     2521 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
     2581 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgaatt
     2641 tgggccgtct ctgcaaaagt ctaggcctcc aaaaaagcct cctcactact tctggaatag
     2701 ctcagaggcc gaggcggcct cggcctctgc ataaataaaa aaaattagtc agccatgggg
     2761 cggagaatgg gcggaactgg gcggagttag gggcgggata gctagagcca gacatgataa
     2821 gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt
     2881 gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagttc
     2941 ctctcactct ctgatattca tttctttgca agttataaat actgaataat aagatgacat
     3001 gaactactac tgctagagat tttccacact gactaaaagg gtctgaggga tctctagtta
     3061 ccagagtcac acaacagacg ggcacacact acttgaagca ctcaaggcaa gctttattga
     3121 ggcttaagca gtgggttccc tagttagcca gagagctccc aggctcagat ctggtctaac
     3181 cagagagacc cagtacaagc aaaaagcaga tcttattttc gttgggagtg aattagccct
     3241 tccagtcccc ccttttcttt taaaaagtgg ctaagatcta cagctgcctt gtaagtcatt
     3301 ggtcttaaag gtaccgagct cgaattaatt ccaggcgggg aggcggccca aagggagatc
     3361 cgactcgtct gagggcgaag gcgaagatgc ggaagaggcc gcagagccgg cagcaggccg
     3421 cgggaaggaa ggtccgctgg attgagggcc gaagggacgt agcagaagga cgtcccgcgc
     3481 agaatccagg tggcaacaca ggcgagcagc catggaaagg acgtcagctt ccccgacaac
     3541 accacggaat tgtcagtgcc caacagccga gcccctgtcc agcagcgggc aaggcaggcg
     3601 gcgatgagtt ccgccgtggc aatagggagg gggaaagcga aagtcccgga aaggagctga
     3661 caggtggtgg caatgcccca accagtgggg gttgcgtcag caaacacagt gcacaccacg
     3721 ccacgttgcc tgacaacggg ccacaactcc tcataaagag acagcaacca ggatttatac
     3781 aaggaggaga aaatgaaagc catacgggaa gcaatagcat gatacaaagg cattaaagca
     3841 gcgtatccac atagcgtaaa aggagcaaca tagttaagaa taccagtcaa tctttcacaa
     3901 attttgtaat ccagaggttg attgtcgagt cgactagtcc tgttcctcag ctacaaaatg
     3961 gacacaattt ccagcagggt ctctgagggc aaattccctt ccccaaggtt gttcaccaat
     4021 ttctgtcatg gctgggccag aggcatccct gaaatttgtg ctgactactt ctgaccattc
     4081 tgcataaagc tcatctaggc ctctgaccca gacccaagca agggtgttgt cagggacaac
     4141 ttggtcctga actgctgaga tgaagagggt gacatcatct ctgacaacac cagcaaaatc
     4201 atcttcaaca aagtctctgg agaatcctaa tctgtcagtc cagaactcta cagcccctgc
     4261 aacatccctt gctgtgagga ctgggactgc agaagtgagt ttggccatac cggaagggcc
     4321 gggattctcc tccacgtcac cgcatgttag aagacttcct ctgccctcac cggtcacatt
     4381 gatcctagca gaagcacagg ctgcagggtg acggtccatc ccgctctcct gggcacaaga
     4441 catgggcagc gtgccatcat cctgcgccgc caccgccggc gggaagccat ggctgatatc
     4501 tcgagatctg gcgaaggcga tgggggtctt gaaggcgtgc tggtactcca cgatgcccag
     4561 ctcggtgttg ctgtgcagct cctccacgcg gcggaaggcg aacatggggc ccccgttctg
     4621 caggatgctg gggtggatgg cgctcttgaa gtgcatgtgg ctgtccacca cgaagctgta
     4681 gtagccgccg tcgcgcaggc tgaaggtgcg ggcgaagctg cccaccagca cgttatcgcc
     4741 catggggtgc aggtgctcca cggtggcgtt gctgcggatg atcttgtcgg tgaagatcac
     4801 gctgtcctcg gggaagccgg tgcccaccac cttgaagtcg ccgatcacgc ggccggcctc
     4861 gtagcggtag ctgaagctca cgtgcagcac gccgccgtcc tcgtacttct cgatgcgggt
     4921 gttggtgtag ccgccgttgt tgatggcgtg caggaagggg ttctcgtagc cgctggggta
     4981 ggtgccgaag tggtagaagc cgtagcccat cacgtggctc agcaggtagg ggctgaaggt
     5041 cagggcgcct ttggtgctct tcatcttgtt ggtcatgcgg ccctgctcgg gggtgccctc
     5101 tccgccgccc accagctcga actccacgcc gttcagggtg ccggtgatgc ggcactcgat
     5161 cttcatggcg ggcatggtgg cggatccaac gaatgtcgag aggctggatc ggtcccggtg
     5221 tcttctatgg aggtcaaaac agcgtggatg gcgtctccag gcgatctgac ggttcactaa
     5281 acgagctctg cttatatagg cctcccaccg tacacgccta actagttcta gaattcatcg
     5341 ataaaatttt gaattttgta atttgttttt gtaattcttt agtttgtatg tctgttgcta
     5401 ttatgtctac tattctttcc cctgcactgt accccccaat cccccctttt cttttaaaag
     5461 ttaaccgata ccgtcgagat ccgttcacta atcgaatgga tctgtctctg tctctctctc
     5521 caccttcttc ttctattcct tcgggcctgt cgggtcccct cggggttggg aggtgggtct
     5581 gaaacgataa tggtgaatat ccctgcctaa ctctattcac tatagaaagt acagcaaaaa
     5641 ctattcttaa acctaccaag cctcctacta tcattatgaa taattttata taccacagcc
     5701 aatttgttat gttaaaccaa ttccacaaac ttgcccattt atctaattcc aataattctt
     5761 gttcattctt ttcttgctgg ttttgcgatt cttcaattaa ggagtgtatt aagcttgtgt
     5821 aattgttaat ttctctgtcc cactccatcc aggtcgtgtg attccaatct gttccagaga
     5881 tttattactc caactagcat tccaaggcac agcagtggtg caaatgagtt ttccagagca
     5941 accccaaatc cccaggagct gttgatcctt taggtatctt tccacagcca ggattcttgc
     6001 ctggagctgc ttgatgcccc agactgtgag ttgcaacaga tgctgttgcg cctcaatagc
     6061 cctcagcaaa ttgttctgct gctgcactat accagacaat aattgtctgg cctgtaccgt
     6121 cagcgtcatt gaggctgcgc ccatagtgct tcctgctgct cccaagaacc caaggaacaa
     6181 agctcctatt cccactgctc ttttttctct ctgcaccact cttctctttg ccttggtggg
     6241 tgctactcct aatggttcaa tttttactac tttatattta tataattcac ttctccaatt
     6301 gtccctcata tctcctcctc caggtctgaa gatcagcggc cgcttgctgt gcggtggtct
     6361 tacttttgtt ttgctcttcc tctatcttgt ctaaagcttc cttggtgtct tttatctcta
     6421 tcctttgatg cacacaatag agggttgcta ctgtattata taatgatcta agttcttctg
     6481 atcctgtctg aagggatggt tgtagctgtc ccagtatttg tctacagcct tctgatgttt
     6541 ctaacaggcc aggattaact gcgaatcgtt ctagctccct gcttgcccat actatatgtt
     6601 ttaatttata ttttttcttt ccccctggcc ttaaccgaat tttttcccat cgcgatctaa
     6661 ttctcccccg cttaatactg acgctctcgc acccatctct ctccttctag cctccgctag
     6721 tcaaaatttt tggcgtactc accagtcgcc gcccctcgcc tcttgccgtg cgcgcttcag
     6781 caagccgagt cctgcgtcga gagagctctg gtttcccttt cgctttcagg tccctgttcg
     6841 ggcgccactg ctagagattt tccacactga ctaaaagggt ctgagggatc tctagttacc
     6901 agagtcacac aacagacggg cacacactac ttgaagcact caaggcaagc tttattgagg
     6961 cttaagcagt gggttcccta gttagccaga gagctcccag gctcagatct ggtctaacca
     7021 gagagacccg tttattgtat cgagctaggc acttaaatac aatatctctg caatgcggca
     7081 attcagtggt tcgtccaatc catgtcagac ccgtctgttg ccttcctaat aaggcacgat
     7141 cgtaccacct tacttccacc aatcggcatg cacggtgctt tttctctcct tgtaaggcat
     7201 gttgctaact catcgttacc atgttgcaag actacaagag tattgcataa gactacacgc
     7261 gt
//
