LOCUS       40924_23989       14976 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pGZ-DSB-CO, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14976)
  AUTHORS   Zapotoczny G, Sekelsky J.
  TITLE     Human Cell Assays for Synthesis-Dependent Strand Annealing and 
            Crossing Over During Double-Strand Break Repair
  JOURNAL   G3 (Bethesda) (2017) In press
  PUBMED    28179392
REFERENCE   2  (bases 1 to 14976)
  AUTHORS   Zapotoczny G, Sekelsky J.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2017) Department of Biology, University of North 
            Carolina, 133 N Medical Dr, Chapel Hill, NC 27599, USA
REFERENCE   3  (bases 1 to 14976)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 14976)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda) (2017) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (10-JAN-2017) Department of Biology, University of North Carolina, 
            133 N Medical Dr, Chapel Hill, NC 27599, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..14976
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      116..496
                     /label=CMV immediate-early enhancer
                     /note="CMV immediate-early enhancer"
                     /regulatory_class="enhancer"
     enhancer        116..495
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        498..773
                     /label=chicken beta-actin promoter
     intron          774..1782
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and
                     rabbit beta-globin"
     CDS             1867..1887
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     CDS             1909..1929
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     regulatory      2143..2152
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    2991..3112
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(3776..4372)
                     /label=PuroR
                     /note="puromycin N-acetyltransferase"
     promoter        complement(4391..4888)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     polyA_signal    5041..5096
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     CDS             complement(5448..6155)
                     /label=mCherry
                     /note="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
     CDS             complement(6505..7221)
                     /label=EGFP
                     /note="enhanced GFP"
     polyA_signal    complement(7422..7503)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    8052..8070
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    complement(8078..8096)
                     /label=tet operator
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        complement(8097..8294)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     enhancer        complement(8296..8675)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     complement(8770..8786)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    8794..8810
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(8818..8848)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(8863..8884)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      8989..9124
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     misc_feature    9128..10847
                     /label=mouse HPRT homology arm
                     /note="mouse HPRT homology arm"
     promoter        complement(10884..10902)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(10909..10925)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      11067..11522
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        11548..11652
                     /label=AmpR promoter
     CDS             11653..12510
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      12684..13272
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    13560..13581
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        13596..13626
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    13634..13650
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     13658..13674
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        13695..13713
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     13750..13766
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    13785..14976
                     /label=mouse HPRT homology arm
                     /note="mouse HPRT homology arm"
ORIGIN
        1 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
       61 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tggtcgacat
      121 tgattattga ctagttatta atagtaatca attacggggt cattagttca tagcccatat
      181 atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac
      241 ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc
      301 cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg
      361 tatcatatgc caagtccgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat
      421 tatgcccagt acatgacctt acgggacttt cctacttggc agtacatcta cgtattagtc
      481 atcgctatta ccatgggtcg aggtgagccc cacgttctgc ttcactctcc ccatctcccc
      541 cccctcccca cccccaattt tgtatttatt tattttttaa ttattttgtg cagcgatggg
      601 ggcggggggg gggggggcgc gcgccaggcg gggcggggcg gggcgagggg cggggcgggg
      661 cgaggcggag aggtgcggcg gcagccaatc agagcggcgc gctccgaaag tttcctttta
      721 tggcgaggcg gcggcggcgg cggccctata aaaagcgaag cgcgcggcgg gcgggagtcg
      781 ctgcgttgcc ttcgccccgt gccccgctcc gcgccgcctc gcgccgcccg ccccggctct
      841 gactgaccgc gttactccca caggtgagcg ggcgggacgg cccttctcct ccgggctgta
      901 attagcgctt ggtttaatga cggctcgttt cttttctgtg gctgcgtgaa agccttaaag
      961 ggctccggga gggccctttg tgcggggggg agcggctcgg ggggtgcgtg cgtgtgtgtg
     1021 tgcgtgggga gcgccgcgtg cggcccgcgc tgcccggcgg ctgtgagcgc tgcgggcgcg
     1081 gcgcggggct ttgtgcgctc cgcgtgtgcg cgaggggagc gcggccgggg gcggtgcccc
     1141 gcggtgcggg ggggctgcga ggggaacaaa ggctgcgtgc ggggtgtgtg cgtggggggg
     1201 tgagcagggg gtgtgggcgc ggcggtcggg ctgtaacccc cccctgcacc cccctccccg
     1261 agttgctgag cacggcccgg cttcgggtgc ggggctccgt gcggggcgtg gcgcggggct
     1321 cgccgtgccg ggcggggggt ggcggcaggt gggggtgccg ggcggggcgg ggccgcctcg
     1381 ggccggggag ggctcggggg aggggcgcgg cggccccgga gcgccggcgg ctgtcgaggc
     1441 gcggcgagcc gcagccattg ccttttatgg taatcgtgcg agagggcgca gggacttcct
     1501 ttgtcccaaa tctggcggag ccgaaatctg ggaggcgccg ccgcaccccc tctagcgggc
     1561 gcgggcgaag cggtgcggcg ccggcaggaa ggaaatgggc ggggagggcc ttcgtgcgtc
     1621 gccgcgccgc cgtccccttc tccatctcca gcctcggggc tgccgcaggg ggacggctgc
     1681 cttcgggggg gacggggcag ggcggggttc ggcttctggc gtgtgaccgg cggctctaga
     1741 gcctctgcta accatgttca tgccttcttc tttttcctac agctcctggg caacgtgctg
     1801 gttattgtgc tgtctcatca ttttggcaaa gaattcagat ccgccgccac tatgggatca
     1861 agatcgccaa aaaagaagag aaaggtgccg aagaagcatg cagcaccacc aaaaaaaaaa
     1921 cgaaaagtag aagacccacg aggcaacacc agcggcgtgc tgagcacccc caaggccaag
     1981 agggccaagc acccccccgg caccgagaag cccaggagca ggagccagag cgagcagccc
     2041 gccacctgcc ccatctgcta cgccgtgatc aggcagagca ggaacctgag gaggcacctg
     2101 gagctgaggc acttcgccaa gcccggcgtg gatccaccgg tcgccaccat ggtgagcaag
     2161 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac
     2221 ggccacaagt tcagcgtgtc cggctaggga taacagggta atacctacgg caagctgacc
     2281 ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc
     2341 ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc
     2401 ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac
     2461 ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc
     2521 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac
     2581 aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg
     2641 aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag
     2701 cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc
     2761 cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc
     2821 gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagtaaag cggccgcgac
     2881 tctagatcat aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc
     2941 cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta
     3001 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     3061 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct taaggcgtaa
     3121 attgtaagcg ttaatctgaa gcctctgatg gaattagaac ttggcaaaac aatactgaga
     3181 atgaagtgta tgtggaacag aggctgctga tctcgttctt caggctatga aactgacaca
     3241 tttggaaacc acagtactta gaaccacaaa gtgggaatca agagaaaaac aatgatccca
     3301 cgagagatct atagatctat agatcatgag tgggaggaat gagctggccc ttaatttggt
     3361 tttgcttgtt taaattatga tatccaacta tgaaacatta tcataaagca atagtaaaga
     3421 gccttcagta aagagcaggc atttatctaa tcccacccca cccccacccc cgtagctcca
     3481 atccttccat tcaaaatgta ggtactctgt tctcaccctt cttaacaaag tatgacagga
     3541 aaaacttcca ttttagtgga catctttatt gtttaataga tcatcaattt cgatccgctc
     3601 ctgggcaccg aactgcgccg cgtgttcagc agggtcggcg tgttcggtgt gtcccccgcg
     3661 gtgggcctcg ggggcgggtg cggggtcggc ggggccgccc cgggtggctt cggtcggagc
     3721 catggggtcg tgcgctcctt tcggtcgggc gctgcgggtc gtggggcggg cgtcaggcac
     3781 cgggcttgcg ggtcatgcac caggtgcgcg gtccttcggg cacctcgacg tcggcggtga
     3841 cggtgaagcc gagccgctcg tagaagggga ggttgcgggg cgcggaggtc tccaggaagg
     3901 cgggcacccc ggcgcgctcg gccgcctcca ctccggggag cacgacggcg ctgcccagac
     3961 ccttgccctg gtggtcgggc gagacgccga cggtggccag gaaccacgcg ggctccttgg
     4021 gccggtgcgg cgccaggagg ccttccatct gttgctgcgc ggccagccgg gaaccgctca
     4081 actcggccat gcgcgggccg atctcggcga acaccgcccc cgcttcgacg ctctccggcg
     4141 tggtccagac cgccaccgcg gcgccgtcgt ccgcgaccca caccttgccg atgtcgagcc
     4201 cgacgcgcgt gaggaagagt tcttgcagct cggtgacccg ctcgatgtgg cggtccgggt
     4261 cgacggtgtg gcgcgtggcg gggtagtcgg cgaacgcggc ggcgagggtg cgtacggccc
     4321 gggggacgtc gtcgcgggtg gcgaggcgca ccgtgggctt gtactcggtc atggtggcgg
     4381 ctggatcggt cgaaaggccc ggagatgagg aagaggagaa cagcgcggca gacgtgcgct
     4441 tttgaagcgt gcagaatgcc gggcctccgg aggaccttcg ggcgcccgcc ccgcccctga
     4501 gcccgcccct gagcccgccc ccggacccac cccttcccag cctctgagcc cagaaagcga
     4561 aggagcaaag ctgctattgg ccgctgcccc aaaggcctac ccgcttccat tgctcagcgg
     4621 tgctgtccat ctgcacgaga ctagtgagac gtgctacttc catttgtcac gtcctgcacg
     4681 acgcgagctg cggggcgggg gggaacttcc tgactagggg aggagtagaa ggtggcgcga
     4741 aggggccacc aaagaacgga gccggttggc gcctaccggt ggatgtggaa tgtgtgaggc
     4801 cagaggccac ttgtgtagcg ccaagtgccc agcggggctg ctaaagcgca tgctccagac
     4861 tgccttggga aaagcgcctc ccctacccgg tagaattcac tcctcaggtg caggctgcct
     4921 atcagaaggt ggtggctggt gtggccaatg ccctggctca caaataccac tgagatcttt
     4981 ttccctctgc caaaaattat ggggacatca tgaagcccct tgagcatctg acttctggct
     5041 aataaaggaa atttattttc attgcaatag tgtgttggaa ttttttgtgt ctctcactcg
     5101 gaaggacata tgggagggca aatcatttaa aacatcagaa tgagtatttg gtttagagtt
     5161 tggcaacata tgccatatgc tggctgccat gaacaaaggt ggctataaag aggtcatcag
     5221 tatatgaaac agccccctgc tgtccattcc ttattccata gaaaagcctt gacttgaggt
     5281 tagatttttt ttatattttg ttttgtgtta tttttttctt taacatccct aaaattttcc
     5341 ttacatgttt tactagccag atttttcctc ctctcctgac tactcccagt catagctgtc
     5401 cctcttctct tatgaagatc cctcgacctg caggccgcac cggtctactt gtacagctcg
     5461 tccatgccgc cggtggagtg gcggccctcg gcgcgttcgt actgttccac gatggtgtag
     5521 tcctcgttgt gggaggtgat gtccaacttg atgttgacgt tgtaggcgcc gggcagctgc
     5581 acgggcttct tggccttgta ggtggtcttg acctcagcgt cgtagtggcc gccgtccttc
     5641 agcttcagcc tctgcttgat ctcgcccttc agggcgccgt cctcggggta catccgctcg
     5701 gaggaggcct cccagcccat ggtcttcttc tgcattacgg ggccgtcgga ggggaagttg
     5761 gtgccgcgca gcttcacctt gtagatgaac tcgccgtcct gcagggagga gtcctgggtc
     5821 acggtcacca cgccgccgtc ctcgaagttc atcacgcgct cccacttgaa gccctcgggg
     5881 aaggacagct tcaagtagtc ggggatgtcg gcggggtgct tcacgtaggc cttggagccg
     5941 tacatgaact gaggggacag gatgtcccag gcgaagggca gggggccacc cttggtcacc
     6001 ttcagcttgg cggtctgggt gccctcgtag gggcggccct cgccctcgcc ctcgatctcg
     6061 aactcgtggc cgttcacgga gccctccatg tgcaccttga agcgcatgaa ctccttgatg
     6121 atggccatgt tatcctcctc gcccttgctc accatggtgg cgcctgcagg acctgtagga
     6181 aaaagaagaa ggcatgaaca tggttagcag aggggcccgg tttggactca gagtatttta
     6241 tcctcatctc aaacagtgta tatcattgta accataaaga gaaaggcagg atgatgacca
     6301 ggatgtagtt gtttctacca ataagaatat ttccacgcca gccagaattt atatgcagaa
     6361 atattctacc ttatcattta attataacaa ttgcattcat tttatgtttc aggttcaggg
     6421 ggaggtgtgg gaggtttttt aaagcaagta aaacctctac aaatgtggta tggctgatta
     6481 tgatctagag tcgcggccgc tttacttgta cagctcgtcc atgccgagag tgatcccggc
     6541 ggcggtcacg aactccagca ggaccatgtg atcgcgcttc tcgttggggt ctttgctcag
     6601 ggcggactgg gtgctcaggt agtggttgtc gggcagcagc acggggccgt cgccgatggg
     6661 ggtgttctgc tggtagtggt cggcgagctg cacgctgccg tcctcgatgt tgtggcggat
     6721 cttgaagttc accttgatgc cgttcttctg cttgtcggcc atgatataga cgttgtggct
     6781 gttgtagttg tactccagct tgtgccccag gatgttgccg tcctccttga agtcgatgcc
     6841 cttcagctcg atgcggttca ccagggtgtc gccctcgaac ttcacctcgg cgcgggtctt
     6901 gtagttgccg tcgtccttga agaagatggt gcgctcctgg acgtagcctt cgggcatggc
     6961 ggacttgaag aagtcgtgct gcttcatgtg gtcggggtag cggctgaagc actgcacgcc
     7021 gtaggtcagg gtggtcacga gggtgggcca gggcacgggc agcttgccgg tggtgcagat
     7081 gaacttcagg gtcagcttgc cgtaggtggc atcgccctcg ccctcgccgg acacgctgaa
     7141 cttgtggccg tttacgtcgc cgtccagctc gaccaggatg ggcaccaccc cggtgaacag
     7201 ctcctcgccc ttgctcacca tggtggcgac cggtggatcc acgccgggct tggcgaagtg
     7261 cctcagctcc aggtgcctcc tcaggttcct gctctgcctg atcacggcgt agcagatggg
     7321 gcaggtggcg ggctgctcgc tctggctcct gctcctgggc ttctcggtgc cgggggggtg
     7381 cttggccctc ttaagcttgg gctgcagacc acaactagaa tgcagtgaaa aaaatgcttt
     7441 atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa
     7501 gttaacaaca acaattgttc tctaaaactg tgctgaagta caatataata taccctgatt
     7561 gccttgaaaa aaaagtgatt agagaaagta cttacaatct gacaaataaa caaaagtgaa
     7621 tttaaaaatt cgttacaaat gcaagctaaa gtttaacgaa aaagttacag aaaatgaaaa
     7681 gaaaataaga ggagacaatg gttgtcaaca gagtagaaag tgaaagaaac aaaattatca
     7741 tgagggtcca tggtgataca agggacatct tcccattcta aacaacaccc tgaaaacttt
     7801 gccccctcca tataacatga attttacaat agcgaaaaag aaagaacaat caagggtccc
     7861 caaactcacc ctgaagttct caggatcgat ccggagcttt ttgcaaaagc ctaggcctcc
     7921 aaaaaagcct cttcactact tctggaatag ctcagaggcc ctagaggatc cccgggtacc
     7981 aagcttaagt ttaaacgcta gagtccggag gctggatcgg tcccggtgtc ttctatggag
     8041 gtcaaaacag ctctctatca ctgataggga gatctgatct ctatcactga tagggatgct
     8101 tatatagacc tcccaccgta cacgcctacc gcccatttgc gtcaatgggg cggagttgtt
     8161 acgacatttt ggaaagtccc gttgattttg gtgccaaaac aaactcccat tgacgtcaat
     8221 ggggtggaga cttggaaatc cccgtgagtc aaaccgctat ccacgcccat tgatgtactg
     8281 ccaaaaccgc atcaccatgg taatagcgat gactaatacg tagatgtact gccaagtagg
     8341 aaagtcccat aaggtcatgt actgggcata atgccaggcg ggccatttac cgtcattgac
     8401 gtcaataggg ggcgtacttg gcatatgata cacttgatgt actgccaagt gggcagttta
     8461 ccgtaaatac tccacccatt gacgtcaatg gaaagtccct attggcgtta ctatgggaac
     8521 atacgtcatt attgacgtca atgggcgggg gtcgttgggc ggtcagccag gcgggccatt
     8581 taccgtaagt tatgtaacgc ggaactccat atatgggcta tgaactaatg accccgtaat
     8641 tgattactat taataactag tcaataatca atgtcaacgc gtatatctgg cccgtacatc
     8701 gcgaagcagc gcaaaacgcc taaccctaag cagattcttc atgcaattta aaagcttggc
     8761 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
     8821 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
     8881 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagcggat
     8941 ccgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta
     9001 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
     9061 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga
     9121 ggcctagagc ttgtttttat ttgtatcttt aaattttatg atagatgtta tagtgtactc
     9181 tcctctcccc tcccctcccc tctcctcctc agcaggatct tgttatgtag ccctaaggga
     9241 tctaatactt actctgtagt ccaggctgtc cttcagctca tgagacacac catcatgctt
     9301 ggcatgtttc ttgttctgaa aggtatctgg ttttaatttt ggttgagctg gtgtgttggt
     9361 gtatatctgt aatctcagca caggcaagta gagacaagga ttatgattac agtccagcct
     9421 gggctattta gagggattct ctttaatctt tcctcatgcc ccaaaatctt acctttggta
     9481 tatgaaaaat agtctccact tctgcaaaat attgctttat gaagtaagaa ttcccttcat
     9541 agagacaagg aatgtgtcct gtaaaagttt aatgtgtaag aagtatttgt tataaaagat
     9601 aaatattcag aatcttcttt ttaattcctg attttatttc tataggactg aaagacttgc
     9661 tcgagatgtc atgaaggaga tgggaggcca tcacattgtg gccctctgtg tgctcaaggg
     9721 gggctataag ttctttgctg acctgctgga ttacattaaa gcactgaata gaaatagtga
     9781 tagatccatt cctatgactg tagattttat cagactgaag agctactgtg taagtataat
     9841 taacttataa ttaaaaaaat agggccattc tagttttatc tatatttttt ttaaacttgt
     9901 gcaaactatg ctacataatc aacttacttg attatgtaat atatatagta atagttggaa
     9961 ttactattat tacatattta tttttctgtg ctggggtaga acccagggaa ctaagtactg
    10021 taataaccga gcttcattca tcctagtcat ttttaatttt tttttttttt tttgtttttt
    10081 cgagacaagg tttctctgtg tagccctggc tgtcctggaa ctcactctgt agaccaggct
    10141 ggcctcgaac tcagaaatct gcctgcctct gcctcccagt gctgagatta atgacgtgca
    10201 ccaccactgc ctggcatttt tttaaagttt taatttgaga ccctttctca ctaacttgct
    10261 cacactatcc tcgaactcat tacgtgggct ggtctagaaa tttcagtcaa cttgcctcag
    10321 cttctcaagt agctgggatt acaggcaggt gctaccactt ctggccatga tcattgttgt
    10381 tgttgctatt attattatta ttatttcact gatacttcat atcacaacac agaatagtaa
    10441 ctgtagttca tgaccaacaa aaaagttgta cagtttctct gttgtcttat tttgatctgg
    10501 attttgattt gtttcatggc actgggcagg ctaatctcaa actgcatggc tcaaatagtc
    10561 cttctgtctt aatctcccat gttctggaag tataggtatc actacaccta gctgtatttt
    10621 catattttaa aaaaaacgcc taagaattgc tattgaatga ggcggagaga tggctcagca
    10681 gtcacaagta cttgtagagg actggggttt ggttactagc acctcaaaga tggcttacaa
    10741 ttatctgtag ctccaattcc agaggatctg tgtggtaaag ctgcatgtat atgggcatgc
    10801 aggtaaaact gctaaacata taaaaatagc tcagtcttaa gagaattggt cgacctcgag
    10861 ggggggcccg gtacccaatt cgccctatag tgagtcgtat tacaattcac tggccgtcgt
    10921 tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca
    10981 tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca
    11041 gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg
    11101 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
    11161 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
    11221 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
    11281 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
    11341 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
    11401 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa
    11461 aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat
    11521 ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
    11581 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
    11641 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
    11701 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
    11761 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
    11821 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
    11881 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct
    11941 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
    12001 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
    12061 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
    12121 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
    12181 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
    12241 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
    12301 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
    12361 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
    12421 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
    12481 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
    12541 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
    12601 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
    12661 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
    12721 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
    12781 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg
    12841 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
    12901 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
    12961 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
    13021 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
    13081 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
    13141 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
    13201 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
    13261 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
    13321 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
    13381 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
    13441 gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
    13501 taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt
    13561 aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt
    13621 atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat
    13681 tacgccaagc tcgaaattaa ccctcactaa agggaacaaa agctggagct ccaccgcggt
    13741 ggcggccgct ctagaactag tggatccgcg gccgctaatt cgattcttct gtaaaattat
    13801 cttattagta agttgtaacc atctcagact tctaagtttg agtttgtaaa ggatcagttt
    13861 tttttggtga cctacacaca cacacacaca cacacacaca cacacacaca caggggggtg
    13921 gattctgaga atttagagaa tttagatggt taataccagt ttttattttt attctgtgtg
    13981 atatttattt attaagattt atttatttat tacatgtagg tacactgtat ctgtcttcag
    14041 acacaccaga agagggcatc agatctcgtt acagatggtt gtgagtcacc atgtggttgc
    14101 tgggatttga actcaggacc tctggaagaa cagtcagtgc tcttaaccgt tgagccatct
    14161 caccagcccc gtgatatttt atttaactta ctagaatata ctgtgttaaa acatatttta
    14221 ggggattggg gagatggccc attctgtaaa gtgtctgcca tgtcatcttc aggacctgcg
    14281 tttgtaaccc cagcatggag aagacagaga cagggagacc cctgggcttc ctggcaagct
    14341 agactagcaa atttggttaa ctaggttcaa tgagagactc agtctcaaaa cataagaagt
    14401 gaatttttta gacccaattt caatctctga actccatacg tatgcacatg tacctgcata
    14461 taaatatata tacacaccca taaacacatg cacacacaaa aaatggttca aaagttctta
    14521 aaatgtataa gagtagaaag tgtagagccg ggagtggtgg cgcacgcctt taatcccagc
    14581 acttgggagc agaggcaggt ggatttctga gttcgaggcc agcctggtct acagagtgag
    14641 ttccaggaca gccagagcta tacagagaaa tcctgtctca aaaaaccaaa aataaagtgt
    14701 atatactaat ctttcatgcc taaaatattt atttttgcca catttttcat acctctttaa
    14761 tatttttctt tttctctttg tgcttcaaag gaaattttgt gtcatgtttt acttttcatt
    14821 cttgactcta ttcagcagta agacgcagca tggctttctt agagaagtat aatctattac
    14881 aaaacggtaa catttaactg ctctacatac cctcttgtag ctaaattcct ctgttaaact
    14941 aagacttatt atctaaacgt aattaaatgt tgatat
//
